ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd2n23
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1KEULE_ARATH (Q9C5X3) SNARE-interacting protein KEULE 129 3e-30
2SEC1B_ARATH (Q9SZ77) Protein transport Sec1b (AtSec1b) 103 2e-22
3SEC1A_ARATH (Q9C5P7) Protein transport Sec1a (AtSec1a) 82 8e-16
4ROP_DROME (Q07327) Protein ROP 44 2e-04
5STXB3_MOUSE (Q60770) Syntaxin-binding protein 3 (UNC-18 homolog ... 43 3e-04
6STXB2_MOUSE (Q64324) Syntaxin-binding protein 2 (UNC-18 homolog ... 42 5e-04
7STXB3_HUMAN (O00186) Syntaxin-binding protein 3 (UNC-18 homolog ... 42 5e-04
8STXB2_HUMAN (Q15833) Syntaxin-binding protein 2 (Unc-18 homolog ... 42 7e-04
9UNC18_CAEEL (P34815) Putative acetylcholine regulator unc-18 (Un... 42 0.001
10STXB2_RAT (Q62753) Syntaxin-binding protein 2 (Unc-18 homolog 2)... 41 0.001
11STXB2_CANFA (Q28288) Syntaxin-binding protein 2 (Unc-18 homolog ... 41 0.001
12STXB1_RAT (P61765) Syntaxin-binding protein 1 (Unc-18 homolog) (... 38 0.013
13STXB1_MOUSE (O08599) Syntaxin-binding protein 1 (Unc-18 homolog)... 38 0.013
14STXB1_HUMAN (P61764) Syntaxin-binding protein 1 (Unc-18 homolog)... 38 0.013
15STXB1_BOVIN (P61763) Syntaxin-binding protein 1 (Unc-18 homolog)... 38 0.013
16SEC1_YEAST (P30619) Protein transport protein SEC1 36 0.050
17HIS4_GEOKA (Q5KVC9) 1-(5-phosphoribosyl)-5-[(5-phosphoribosylami... 32 0.95
18POLG_HCVBB (Q68749) Genome polyprotein [Contains: Core protein p... 31 1.2
19HIS4_BACSU (O35006) 1-(5-phosphoribosyl)-5-[(5-phosphoribosylami... 31 1.2
20POLG_HCVJ6 (P26660) Genome polyprotein [Contains: Core protein p... 30 2.1
21POLG_HCVJF (Q99IB8) Genome polyprotein [Contains: Core protein p... 30 3.6
22MUTS2_STAHJ (Q4L5E9) MutS2 protein 29 6.2
23Y3654_ANASP (Q8YR05) Hypothetical RNA methyltransferase alr3654 ... 29 6.2
24POLG_HCVVA (Q9QAX1) Genome polyprotein [Contains: Core protein p... 28 8.1
25YICG_SHIFL (P0AGM4) UPF0126 inner membrane protein yicG 28 8.1
26YICG_ECOLI (P0AGM2) UPF0126 inner membrane protein yicG 28 8.1
27YICG_ECOL6 (P0AGM3) UPF0126 inner membrane protein yicG 28 8.1
28VPS45_YEAST (P38932) Vacuolar protein sorting-associated protein 45 28 8.1

>KEULE_ARATH (Q9C5X3) SNARE-interacting protein KEULE|
          Length = 666

 Score =  129 bits (324), Expect = 3e-30
 Identities = 63/83 (75%), Positives = 77/83 (92%), Gaps = 1/83 (1%)
 Frame = -3

Query: 447 DGYSSDSVLKHASSDFQKHGQRIFVFVIGGAXRSELRXAHKLTSKLKREVILGSSSLDDP 268
           DGYSSDSVL+HASSDF+K GQRIFVF++GGA RSEL+  HKL++KLKREVILGS+SLDDP
Sbjct: 584 DGYSSDSVLRHASSDFRKMGQRIFVFIVGGATRSELKVCHKLSTKLKREVILGSTSLDDP 643

Query: 267 PQFITKLKMMSA-EELTLDDLHI 202
           PQFITKLK+++A ++L+LDDL I
Sbjct: 644 PQFITKLKLLTANDDLSLDDLQI 666



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>SEC1B_ARATH (Q9SZ77) Protein transport Sec1b (AtSec1b)|
          Length = 662

 Score =  103 bits (258), Expect = 2e-22
 Identities = 54/83 (65%), Positives = 65/83 (78%), Gaps = 1/83 (1%)
 Frame = -3

Query: 447 DGYSSDSVLKHASSDFQKHGQRIFVFVIGGAXRSELRXAHKLTSKLKREVILGSSSLDDP 268
           DGY SDSVL  ASS F++ GQRIFVF++GGA RSELR  HKLT KL REVILGSSS  DP
Sbjct: 580 DGYFSDSVLGRASSGFKRKGQRIFVFIVGGATRSELRVCHKLTEKLDREVILGSSSFLDP 639

Query: 267 PQFITKLKMMS-AEELTLDDLHI 202
             F+TK+K ++  EE++LDD+ I
Sbjct: 640 LTFLTKMKQLNEEEEISLDDIVI 662



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>SEC1A_ARATH (Q9C5P7) Protein transport Sec1a (AtSec1a)|
          Length = 611

 Score = 81.6 bits (200), Expect = 8e-16
 Identities = 43/67 (64%), Positives = 51/67 (76%), Gaps = 2/67 (2%)
 Frame = -3

Query: 447 DGYSSDSVLKHASSDFQKHG--QRIFVFVIGGAXRSELRXAHKLTSKLKREVILGSSSLD 274
           DGYS  S+L +   DF   G  +RIFVF+IGGA RSELR  HKLTS L+REV+LGS+S D
Sbjct: 538 DGYSRHSILHN---DFLFFGPFERIFVFIIGGATRSELRVCHKLTSSLRREVVLGSTSFD 594

Query: 273 DPPQFIT 253
           DPPQ+IT
Sbjct: 595 DPPQYIT 601



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>ROP_DROME (Q07327) Protein ROP|
          Length = 597

 Score = 43.5 bits (101), Expect = 2e-04
 Identities = 21/58 (36%), Positives = 39/58 (67%), Gaps = 1/58 (1%)
 Frame = -3

Query: 396 KHGQRIFVFVIGGAXRSELRXAHKLTSKLKR-EVILGSSSLDDPPQFITKLKMMSAEE 226
           K+  R+ VF++GG   SE+R A+++T+ ++  EV++GSS +  P  F++ L  +S E+
Sbjct: 540 KNVPRLIVFIVGGVSMSEMRCAYEVTNAVRNWEVLVGSSHILSPEIFLSDLGSLSKED 597



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>STXB3_MOUSE (Q60770) Syntaxin-binding protein 3 (UNC-18 homolog 3) (UNC-18C)|
           (MUNC-18-3)
          Length = 592

 Score = 43.1 bits (100), Expect = 3e-04
 Identities = 21/56 (37%), Positives = 38/56 (67%), Gaps = 1/56 (1%)
 Frame = -3

Query: 399 QKHGQRIFVFVIGGAXRSELRXAHKLTSKLKR-EVILGSSSLDDPPQFITKLKMMS 235
           +K+G R+ +FVIGG   SE+R A++++   K  EVI+GS+ +  P + +  +KM++
Sbjct: 526 RKNGSRLIIFVIGGITYSEMRCAYEVSQAHKSCEVIIGSTHILTPRKLLDDIKMLN 581



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>STXB2_MOUSE (Q64324) Syntaxin-binding protein 2 (UNC-18 homolog 2) (UNC-18B)|
           (MUSEC1)
          Length = 593

 Score = 42.4 bits (98), Expect = 5e-04
 Identities = 20/53 (37%), Positives = 36/53 (67%), Gaps = 2/53 (3%)
 Frame = -3

Query: 390 GQRIFVFVIGGAXRSELRXAHKLT--SKLKREVILGSSSLDDPPQFITKLKMM 238
           G R+ V+++GG   SE+R A+++T  ++ K EV++GSS +  P +F+  LK +
Sbjct: 531 GPRLIVYIVGGVAMSEMRAAYEVTRATEGKWEVLIGSSHILTPTRFLDDLKTL 583



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>STXB3_HUMAN (O00186) Syntaxin-binding protein 3 (UNC-18 homolog 3) (UNC-18C)|
           (UNC-18-3) (Platelet Sec1 protein) (PSP)
          Length = 592

 Score = 42.4 bits (98), Expect = 5e-04
 Identities = 21/56 (37%), Positives = 38/56 (67%), Gaps = 1/56 (1%)
 Frame = -3

Query: 399 QKHGQRIFVFVIGGAXRSELRXAHKLTSKLKR-EVILGSSSLDDPPQFITKLKMMS 235
           +K+G ++ VFVIGG   SE+R A++++   K  EVI+GS+ +  P + +  +KM++
Sbjct: 525 RKNGSKLIVFVIGGITYSEVRGAYEVSQAHKSCEVIIGSTHVLTPKKLLDDIKMLN 580



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>STXB2_HUMAN (Q15833) Syntaxin-binding protein 2 (Unc-18 homolog 2) (Unc-18B)|
           (Unc18-2)
          Length = 593

 Score = 42.0 bits (97), Expect = 7e-04
 Identities = 21/53 (39%), Positives = 36/53 (67%), Gaps = 2/53 (3%)
 Frame = -3

Query: 390 GQRIFVFVIGGAXRSELRXAHKLT--SKLKREVILGSSSLDDPPQFITKLKMM 238
           G R+ V+V+GG   SE+R A+++T  ++ K EV++GSS +  P +F+  LK +
Sbjct: 531 GPRLIVYVMGGVAMSEMRAAYEVTRATEGKWEVLIGSSHILTPTRFLDDLKAL 583



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>UNC18_CAEEL (P34815) Putative acetylcholine regulator unc-18 (Uncoordinated|
           protein 18)
          Length = 591

 Score = 41.6 bits (96), Expect = 0.001
 Identities = 18/52 (34%), Positives = 34/52 (65%), Gaps = 1/52 (1%)
 Frame = -3

Query: 396 KHGQRIFVFVIGGAXRSELRXAHKLTSKLKR-EVILGSSSLDDPPQFITKLK 244
           + G R+ +++IGG   SE+R  +++T+  K  EV++GS  +  P +F+T L+
Sbjct: 532 RSGPRLIIYIIGGVTFSEMRACYEVTAARKPWEVVIGSDRIITPDKFLTNLR 583



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>STXB2_RAT (Q62753) Syntaxin-binding protein 2 (Unc-18 homolog 2) (Unc-18B)|
           (Unc18-2)
          Length = 594

 Score = 41.2 bits (95), Expect = 0.001
 Identities = 19/53 (35%), Positives = 36/53 (67%), Gaps = 2/53 (3%)
 Frame = -3

Query: 390 GQRIFVFVIGGAXRSELRXAHKLT--SKLKREVILGSSSLDDPPQFITKLKMM 238
           G R+ V+++GG   SE+R A+++T  ++ K EV++GSS +  P +F+  L+ +
Sbjct: 532 GPRLIVYIVGGVAMSEMRAAYEVTRATEGKWEVLIGSSHILTPTRFLDDLRTL 584



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>STXB2_CANFA (Q28288) Syntaxin-binding protein 2 (Unc-18 homolog 2) (Unc-18B)|
           (Unc18-2)
          Length = 593

 Score = 41.2 bits (95), Expect = 0.001
 Identities = 20/53 (37%), Positives = 34/53 (64%), Gaps = 2/53 (3%)
 Frame = -3

Query: 390 GQRIFVFVIGGAXRSELRXAHKLTSKL--KREVILGSSSLDDPPQFITKLKMM 238
           G R+ ++V+GG   SE+R A+++T     K EV++GSS +  P +F+  LK +
Sbjct: 531 GPRLIIYVMGGVAMSEMRAAYEVTRATDGKWEVLIGSSHILTPTRFLDDLKTL 583



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>STXB1_RAT (P61765) Syntaxin-binding protein 1 (Unc-18 homolog) (Unc-18A)|
           (Unc-18-1) (N-Sec1) (rbSec1) (p67)
          Length = 594

 Score = 37.7 bits (86), Expect = 0.013
 Identities = 17/55 (30%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
 Frame = -3

Query: 396 KHGQRIFVFVIGGAXRSELRXAHKLT-SKLKREVILGSSSLDDPPQFITKLKMMS 235
           + G R+ +F++GG   +E+R A+++T +  K EV++GS+ +  P + +  LK ++
Sbjct: 532 RSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLN 586



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>STXB1_MOUSE (O08599) Syntaxin-binding protein 1 (Unc-18 homolog) (Unc-18A)|
           (Unc-18-1)
          Length = 594

 Score = 37.7 bits (86), Expect = 0.013
 Identities = 17/55 (30%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
 Frame = -3

Query: 396 KHGQRIFVFVIGGAXRSELRXAHKLT-SKLKREVILGSSSLDDPPQFITKLKMMS 235
           + G R+ +F++GG   +E+R A+++T +  K EV++GS+ +  P + +  LK ++
Sbjct: 532 RSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLN 586



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>STXB1_HUMAN (P61764) Syntaxin-binding protein 1 (Unc-18 homolog) (Unc-18A)|
           (Unc-18-1) (N-Sec1) (p67)
          Length = 594

 Score = 37.7 bits (86), Expect = 0.013
 Identities = 17/55 (30%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
 Frame = -3

Query: 396 KHGQRIFVFVIGGAXRSELRXAHKLT-SKLKREVILGSSSLDDPPQFITKLKMMS 235
           + G R+ +F++GG   +E+R A+++T +  K EV++GS+ +  P + +  LK ++
Sbjct: 532 RSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLN 586



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>STXB1_BOVIN (P61763) Syntaxin-binding protein 1 (Unc-18 homolog) (Unc-18A)|
           (Unc-18-1) (N-Sec1) (p67)
          Length = 594

 Score = 37.7 bits (86), Expect = 0.013
 Identities = 17/55 (30%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
 Frame = -3

Query: 396 KHGQRIFVFVIGGAXRSELRXAHKLT-SKLKREVILGSSSLDDPPQFITKLKMMS 235
           + G R+ +F++GG   +E+R A+++T +  K EV++GS+ +  P + +  LK ++
Sbjct: 532 RSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLN 586



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>SEC1_YEAST (P30619) Protein transport protein SEC1|
          Length = 724

 Score = 35.8 bits (81), Expect = 0.050
 Identities = 17/60 (28%), Positives = 36/60 (60%), Gaps = 2/60 (3%)
 Frame = -3

Query: 411 SSDFQKH--GQRIFVFVIGGAXRSELRXAHKLTSKLKREVILGSSSLDDPPQFITKLKMM 238
           SS+ +K+   QR F +VIGG    E++ A+  ++   R++ +GS  +  P +F+ +++ +
Sbjct: 580 SSNIKKNIPRQRFFYYVIGGISIPEIKAAYDQSNLKNRDIFIGSDEILTPTKFLDEVERL 639



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>HIS4_GEOKA (Q5KVC9) 1-(5-phosphoribosyl)-5-[(5-|
           phosphoribosylamino)methylideneamino]
           imidazole-4-carboxamide isomerase (EC 5.3.1.16)
           (Phosphoribosylformimino-5-aminoimidazole carboxamide
           ribotide isomerase)
          Length = 245

 Score = 31.6 bits (70), Expect = 0.95
 Identities = 20/48 (41%), Positives = 31/48 (64%), Gaps = 1/48 (2%)
 Frame = -3

Query: 387 QRIFVFV-IGGAXRSELRXAHKLTSKLKREVILGSSSLDDPPQFITKL 247
           +R+ V V +GG  R+E    H L + + R VILGS+++ DPP F+ K+
Sbjct: 74  RRLSVHVQVGGGIRTEEDIVHYLENGVAR-VILGSAAISDPP-FVKKM 119



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>POLG_HCVBB (Q68749) Genome polyprotein [Contains: Core protein p21 (Capsid|
           protein C) (p21); Core protein p19; Envelope
           glycoprotein E1 (gp32) (gp35); Envelope glycoprotein E2
           (NS1) (gp68) (gp70); p7; Protease NS2-3 (EC 3.4.22.-)
           (p23); Serine protease/N
          Length = 3036

 Score = 31.2 bits (69), Expect = 1.2
 Identities = 14/26 (53%), Positives = 17/26 (65%)
 Frame = -2

Query: 193 IMHMYSGASSGILLYFGIFFVIVWFV 116
           I+H  S ASS  LLYF +FFV  W +
Sbjct: 756 ILHAASAASSNGLLYFILFFVAAWCI 781



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>HIS4_BACSU (O35006) 1-(5-phosphoribosyl)-5-[(5-|
           phosphoribosylamino)methylideneamino]
           imidazole-4-carboxamide isomerase (EC 5.3.1.16)
           (Phosphoribosylformimino-5-aminoimidazole carboxamide
           ribotide isomerase)
          Length = 245

 Score = 31.2 bits (69), Expect = 1.2
 Identities = 19/50 (38%), Positives = 31/50 (62%)
 Frame = -3

Query: 366 IGGAXRSELRXAHKLTSKLKREVILGSSSLDDPPQFITKLKMMSAEELTL 217
           IGG  RSE      L++ ++R VILGSS++ +PP F+ K+     E++ +
Sbjct: 82  IGGGIRSENDVYEYLSAGVER-VILGSSAVSNPP-FVKKMLKQYGEKIAI 129



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>POLG_HCVJ6 (P26660) Genome polyprotein [Contains: Core protein p21 (Capsid|
           protein C) (p21); Core protein p19; Envelope
           glycoprotein E1 (gp32) (gp35); Envelope glycoprotein E2
           (NS1) (gp68) (gp70); p7; Protease NS2-3 (EC 3.4.22.-)
           (p23); Serine protease/N
          Length = 3032

 Score = 30.4 bits (67), Expect = 2.1
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = -2

Query: 193 IMHMYSGASSGILLYFGIFFVIVWFV 116
           ++H  S AS    LYF IFFV  W++
Sbjct: 756 VLHAASAASCNGFLYFVIFFVAAWYI 781



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>POLG_HCVJF (Q99IB8) Genome polyprotein [Contains: Core protein p21 (Capsid|
           protein C) (p21); Core protein p19; Envelope
           glycoprotein E1 (gp32) (gp35); Envelope glycoprotein E2
           (NS1) (gp68) (gp70); p7; Protease NS2-3 (EC 3.4.22.-)
           (p23); Serine protease/N
          Length = 3032

 Score = 29.6 bits (65), Expect = 3.6
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = -2

Query: 193 IMHMYSGASSGILLYFGIFFVIVWFV 116
           ++H  S A+   LLYF IFFV  W +
Sbjct: 756 VLHAASAANCHGLLYFAIFFVAAWHI 781



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>MUTS2_STAHJ (Q4L5E9) MutS2 protein|
          Length = 783

 Score = 28.9 bits (63), Expect = 6.2
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +3

Query: 372 KQIYAGHAFGNHSMHASIQNHLNNH 446
           +Q+Y  H  G  ++  ++QNHLN H
Sbjct: 735 EQVYIIHGKGTGALQKAVQNHLNKH 759



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>Y3654_ANASP (Q8YR05) Hypothetical RNA methyltransferase alr3654 (EC 2.1.1.-)|
          Length = 460

 Score = 28.9 bits (63), Expect = 6.2
 Identities = 13/27 (48%), Positives = 18/27 (66%)
 Frame = -1

Query: 134 CDRLVCSTLVASKPHAINLLSCIIFIL 54
           CDR+V  +L+ASKP  I  +SC +  L
Sbjct: 389 CDRIVIESLLASKPARIVYVSCKVATL 415



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>POLG_HCVVA (Q9QAX1) Genome polyprotein [Contains: Core protein p21 (Capsid|
           protein C) (p21); Core protein p19; Envelope
           glycoprotein E1 (gp32) (gp35); Envelope glycoprotein E2
           (NS1) (gp68) (gp70); p7; Protease NS2-3 (EC 3.4.22.-)
           (p23); Serine protease/N
          Length = 3032

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = -2

Query: 193 IMHMYSGASSGILLYFGIFFVIVWFV 116
           I+H  S ASS  +L F IFF+  W++
Sbjct: 756 ILHAASAASSHGMLCFIIFFIAAWYI 781



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>YICG_SHIFL (P0AGM4) UPF0126 inner membrane protein yicG|
          Length = 205

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 9/26 (34%), Positives = 21/26 (80%)
 Frame = +3

Query: 369 QKQIYAGHAFGNHSMHASIQNHLNNH 446
           QK++YAG +F +  ++ ++Q++++NH
Sbjct: 145 QKELYAGVSFASAVLYIALQHYVSNH 170



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>YICG_ECOLI (P0AGM2) UPF0126 inner membrane protein yicG|
          Length = 205

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 9/26 (34%), Positives = 21/26 (80%)
 Frame = +3

Query: 369 QKQIYAGHAFGNHSMHASIQNHLNNH 446
           QK++YAG +F +  ++ ++Q++++NH
Sbjct: 145 QKELYAGVSFASAVLYIALQHYVSNH 170



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>YICG_ECOL6 (P0AGM3) UPF0126 inner membrane protein yicG|
          Length = 205

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 9/26 (34%), Positives = 21/26 (80%)
 Frame = +3

Query: 369 QKQIYAGHAFGNHSMHASIQNHLNNH 446
           QK++YAG +F +  ++ ++Q++++NH
Sbjct: 145 QKELYAGVSFASAVLYIALQHYVSNH 170



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>VPS45_YEAST (P38932) Vacuolar protein sorting-associated protein 45|
          Length = 577

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 14/50 (28%), Positives = 25/50 (50%), Gaps = 2/50 (4%)
 Frame = -3

Query: 387 QRIFVFVIGGAXRSELRXAHKL--TSKLKREVILGSSSLDDPPQFITKLK 244
           Q + +FVIGG    E R  H    T   +  V+LG +S+    +++  ++
Sbjct: 525 QDVILFVIGGVTYEEARLVHDFNGTMNNRMRVVLGGTSILSTKEYMDSIR 574


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,992,499
Number of Sequences: 219361
Number of extensions: 1071588
Number of successful extensions: 2684
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 2647
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2676
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2735358828
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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