ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd2f24
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MA121_DROME (P53624) Mannosyl-oligosaccharide alpha-1,2-mannosid... 89 1e-17
2MA122_DROME (P53625) Mannosyl-oligosaccharide alpha-1,2-mannosid... 89 1e-17
3MA1C1_HUMAN (Q9NR34) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 80 6e-15
4MA1A1_PIG (O02773) Mannosyl-oligosaccharide 1,2-alpha-mannosidas... 77 7e-14
5MA1A2_MOUSE (P39098) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 76 1e-13
6MA1A2_HUMAN (O60476) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 75 1e-13
7MA1A1_MOUSE (P45700) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 74 6e-13
8MA1A1_RABIT (P45701) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 74 6e-13
9MA1A1_HUMAN (P33908) Mannosyl-oligosaccharide 1,2-alpha-mannosid... 71 4e-12
10MA1B1_HUMAN (Q9UKM7) Endoplasmic reticulum mannosyl-oligosacchar... 67 7e-11
11EDEM3_HUMAN (Q9BZQ6) ER degradation-enhancing alpha-mannosidase-... 61 3e-09
12EDEM2_HUMAN (Q9BV94) ER degradation-enhancing alpha-mannosidase-... 60 8e-09
13EDEM1_MOUSE (Q925U4) ER degradation-enhancing alpha-mannosidase-... 55 2e-07
14YH04_YEAST (P38888) Protein YHR204W 55 2e-07
15EDEM1_HUMAN (Q92611) ER degradation-enhancing alpha-mannosidase-... 52 1e-06
16MNS1_CANAL (Q8J0Q0) Mannosyl-oligosaccharide 1,2-alpha-mannosida... 50 7e-06
17MNS1_YEAST (P32906) Endoplasmic reticulum mannosyl-oligosacchari... 49 1e-05
18YKU1_SCHPO (Q9P7C3) Putative mannosyl-oligosaccharide 1,2-alpha-... 49 2e-05
19MAN12_PENCI (P31723) Mannosyl-oligosaccharide alpha-1,2-mannosid... 43 8e-04
20Y1383_METJA (Q58778) Hypothetical protein MJ1383 precursor 30 9.2

>MA121_DROME (P53624) Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform 1|
           (EC 3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 667

 Score = 89.0 bits (219), Expect = 1e-17
 Identities = 49/74 (66%), Positives = 52/74 (70%), Gaps = 2/74 (2%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXVNSGE--KDDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 447
           A EK+ R A GY GLR V   E  KDD  QSFFLAETLKYLYLLFS  SV+  DEWVFNT
Sbjct: 581 ALEKHCRTAHGYCGLRNVYQQEPQKDDVQQSFFLAETLKYLYLLFSDDSVLPLDEWVFNT 640

Query: 446 EAHPLRIVPTHGSN 405
           EAHPL   P  G+N
Sbjct: 641 EAHPL---PIKGAN 651



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>MA122_DROME (P53625) Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform 2|
           (EC 3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 643

 Score = 89.0 bits (219), Expect = 1e-17
 Identities = 49/74 (66%), Positives = 52/74 (70%), Gaps = 2/74 (2%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXVNSGE--KDDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 447
           A EK+ R A GY GLR V   E  KDD  QSFFLAETLKYLYLLFS  SV+  DEWVFNT
Sbjct: 557 ALEKHCRTAHGYCGLRNVYQQEPQKDDVQQSFFLAETLKYLYLLFSDDSVLPLDEWVFNT 616

Query: 446 EAHPLRIVPTHGSN 405
           EAHPL   P  G+N
Sbjct: 617 EAHPL---PIKGAN 627



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>MA1C1_HUMAN (Q9NR34) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IC (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IC)
           (Alpha-1,2-mannosidase IC) (Mannosidase alpha class 1C
           member 1) (HMIC)
          Length = 630

 Score = 80.1 bits (196), Expect = 6e-15
 Identities = 42/80 (52%), Positives = 54/80 (67%), Gaps = 2/80 (2%)
 Frame = -1

Query: 659 DPINNERREKVKXAFEKNSRIASGYVGLRXVNSG--EKDDKMQSFFLAETLKYLYLLFSP 486
           +PI  E   +V  A EK  R  +G+ G++ V S     D+K QSFFLAETLKYLYLLFS 
Sbjct: 538 NPIYREWGWEVVLALEKYCRTEAGFSGIQDVYSSTPNHDNKQQSFFLAETLKYLYLLFSE 597

Query: 485 PSVVSFDEWVFNTEAHPLRI 426
             ++S ++WVFNTEAHPL +
Sbjct: 598 DDLLSLEDWVFNTEAHPLPV 617



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>MA1A1_PIG (O02773) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IA)
           (Alpha-1,2-mannosidase IA) (Mannosidase alpha class 1A
           member 1) (Man(9)-alpha-mannosidase) (Man9-mannosidase)
          Length = 659

 Score = 76.6 bits (187), Expect = 7e-14
 Identities = 37/68 (54%), Positives = 48/68 (70%), Gaps = 2/68 (2%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXV--NSGEKDDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 447
           A EK+ R+  GY GLR V  ++   DD  QSFFLAETLKYLYL+FS   ++  + W+FNT
Sbjct: 580 ALEKHCRVNGGYSGLRDVYVSAQTYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNT 639

Query: 446 EAHPLRIV 423
           EAHPL ++
Sbjct: 640 EAHPLPVL 647



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>MA1A2_MOUSE (P39098) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IB)
           (Alpha-1,2-mannosidase IB) (Mannosidase alpha class 1A
           member 2)
          Length = 641

 Score = 75.9 bits (185), Expect = 1e-13
 Identities = 39/81 (48%), Positives = 52/81 (64%), Gaps = 2/81 (2%)
 Frame = -1

Query: 659 DPINNERREKVKXAFEKNSRIASGYVGLRXVNSGEK--DDKMQSFFLAETLKYLYLLFSP 486
           DP   +   +   A EK+ R++ G+ G++ V +     DD  QSFFLAETLKYLYLLFS 
Sbjct: 547 DPRYRQWGWEAALAIEKSCRVSGGFSGVKDVYAPTPVHDDVQQSFFLAETLKYLYLLFSG 606

Query: 485 PSVVSFDEWVFNTEAHPLRIV 423
             ++  D WVFNTEAHPL ++
Sbjct: 607 DDLLPLDHWVFNTEAHPLPVL 627



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>MA1A2_HUMAN (O60476) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IB)
           (Alpha-1,2-mannosidase IB) (Mannosidase alpha class 1A
           member 2)
          Length = 641

 Score = 75.5 bits (184), Expect = 1e-13
 Identities = 40/81 (49%), Positives = 50/81 (61%), Gaps = 2/81 (2%)
 Frame = -1

Query: 659 DPINNERREKVKXAFEKNSRIASGYVGLRXVNSGEK--DDKMQSFFLAETLKYLYLLFSP 486
           DP   +   +   A EK  R+  G+ G++ V S     DD  QSFFLAETLKYLYLLFS 
Sbjct: 547 DPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSG 606

Query: 485 PSVVSFDEWVFNTEAHPLRIV 423
             ++  D WVFNTEAHPL ++
Sbjct: 607 DDLLPLDHWVFNTEAHPLPVL 627



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>MA1A1_MOUSE (P45700) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IA)
           (Alpha-1,2-mannosidase IA) (Mannosidase alpha class 1A
           member 1) (Man(9)-alpha-mannosidase) (Man9-mannosidase)
          Length = 655

 Score = 73.6 bits (179), Expect = 6e-13
 Identities = 36/68 (52%), Positives = 45/68 (66%), Gaps = 2/68 (2%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXVNSGEK--DDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 447
           A E + R+  GY GLR V    +  DD  QSFFLAETLKYLYL+FS   ++  + W+FNT
Sbjct: 576 ALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNT 635

Query: 446 EAHPLRIV 423
           EAHP  I+
Sbjct: 636 EAHPFPIL 643



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>MA1A1_RABIT (P45701) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IA)
           (Alpha-1,2-mannosidase IA) (Mannosidase alpha class 1A
           member 1) (Man(9)-alpha-mannosidase) (Fragment)
          Length = 469

 Score = 73.6 bits (179), Expect = 6e-13
 Identities = 39/70 (55%), Positives = 49/70 (70%), Gaps = 2/70 (2%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXVN-SGEK-DDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 447
           A E + R+  GY GLR V  + EK D+  QSFFLAETLKYLYL+FS   ++  + W+FNT
Sbjct: 390 ALESHCRVNGGYSGLRDVYFTHEKYDNVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNT 449

Query: 446 EAHPLRIVPT 417
           EAH L I+PT
Sbjct: 450 EAHLLPILPT 459



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>MA1A1_HUMAN (P33908) Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC|
           3.2.1.113) (Processing alpha-1,2-mannosidase IA)
           (Alpha-1,2-mannosidase IA) (Mannosidase alpha class 1A
           member 1) (Man(9)-alpha-mannosidase) (Man9-mannosidase)
          Length = 653

 Score = 70.9 bits (172), Expect = 4e-12
 Identities = 36/69 (52%), Positives = 46/69 (66%), Gaps = 2/69 (2%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXVNSGEK--DDKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNT 447
           A E + R+  GY GLR V    +  DD  QSFFLAETLKYLYL+FS   ++  + W+FN+
Sbjct: 574 ALENHCRVNGGYSGLRDVYLLHESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNS 633

Query: 446 EAHPLRIVP 420
           EAH L I+P
Sbjct: 634 EAHLLPILP 642



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>MA1B1_HUMAN (Q9UKM7) Endoplasmic reticulum mannosyl-oligosaccharide|
           1,2-alpha-mannosidase (EC 3.2.1.113) (ER
           alpha-1,2-mannosidase) (Mannosidase alpha class 1B
           member 1) (Man9GlcNAc2-specific-processing
           alpha-mannosidase)
          Length = 699

 Score = 66.6 bits (161), Expect = 7e-11
 Identities = 37/74 (50%), Positives = 51/74 (68%), Gaps = 5/74 (6%)
 Frame = -1

Query: 632 KVKXAFEKNSRIASG-YVGLRXVNSGEKD---DKMQSFFLAETLKYLYLLFS-PPSVVSF 468
           ++  +F + +R+ SG Y  +  V   +K    DKM+SFFL ETLKYL+LLFS  P+++S 
Sbjct: 622 EILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSL 681

Query: 467 DEWVFNTEAHPLRI 426
           D +VFNTEAHPL I
Sbjct: 682 DAYVFNTEAHPLPI 695



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>EDEM3_HUMAN (Q9BZQ6) ER degradation-enhancing alpha-mannosidase-like 3|
          Length = 889

 Score = 61.2 bits (147), Expect = 3e-09
 Identities = 34/88 (38%), Positives = 51/88 (57%), Gaps = 4/88 (4%)
 Frame = -1

Query: 659 DPINNERREKVKXAFEKNSRIASGYVGLRXVNSGEKDDKMQSFFLAETLKYLYLLFSPPS 480
           DP   E  + +     K +R+  G+  ++ V +G  +D+M SFFLAE  KYLYLLF+   
Sbjct: 376 DPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKE 435

Query: 479 VVSFD--EWVFNTEAH--PLRIVPTHGS 408
            + FD  +++F TEAH  PL +  T+ S
Sbjct: 436 DIIFDIEDYIFTTEAHLLPLWLSTTNQS 463



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>EDEM2_HUMAN (Q9BV94) ER degradation-enhancing alpha-mannosidase-like 2|
           precursor
          Length = 578

 Score = 59.7 bits (143), Expect = 8e-09
 Identities = 34/101 (33%), Positives = 52/101 (51%), Gaps = 19/101 (18%)
 Frame = -1

Query: 659 DPINNERREKVKXAFEKNSRIASGYVGLRXVNSGEKDDKMQSFFLAETLKYLYLLFSPPS 480
           DP   E       + EK S++  G+  ++ +   + D++M+SFFLAET+KYLYLLF P +
Sbjct: 386 DPTLLELGRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTN 445

Query: 479 VV-----SFDE--------------WVFNTEAHPLRIVPTH 414
            +     +FD               ++FNTEAHP+     H
Sbjct: 446 FIHNNGSTFDAVITPYGECILGAGGYIFNTEAHPIDPAALH 486



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>EDEM1_MOUSE (Q925U4) ER degradation-enhancing alpha-mannosidase-like 1|
          Length = 652

 Score = 55.1 bits (131), Expect = 2e-07
 Identities = 28/67 (41%), Positives = 41/67 (61%), Gaps = 2/67 (2%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXVNSGEKDDKMQSFFLAETLKYLYLLFSPPSVV--SFDEWVFNT 447
           + EK +++  GY  L  V    K+D+M+SFFL+ET KYLYLLF   + V  S   ++F T
Sbjct: 515 SLEKYTKVKCGYATLHHVIDKSKEDRMESFFLSETCKYLYLLFDEENPVHKSGTRYMFTT 574

Query: 446 EAHPLRI 426
           E H + +
Sbjct: 575 EGHIISV 581



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>YH04_YEAST (P38888) Protein YHR204W|
          Length = 796

 Score = 55.1 bits (131), Expect = 2e-07
 Identities = 29/68 (42%), Positives = 44/68 (64%), Gaps = 2/68 (2%)
 Frame = -1

Query: 629 VKXAFEKNSRIASGYVGLRXVNSGEKDDKMQSFFLAETLKYLYLLFSPPSVV--SFDEWV 456
           +K  F+ N     G+ G + V +GE  D+M++F L+ETLKYLYLLF   + +  S  + +
Sbjct: 437 LKQRFKSNC----GFAGFQNVITGELQDRMETFVLSETLKYLYLLFDEENELHNSASDVI 492

Query: 455 FNTEAHPL 432
           F+TEAHP+
Sbjct: 493 FSTEAHPM 500



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>EDEM1_HUMAN (Q92611) ER degradation-enhancing alpha-mannosidase-like 1|
          Length = 657

 Score = 52.4 bits (124), Expect = 1e-06
 Identities = 27/63 (42%), Positives = 38/63 (60%), Gaps = 2/63 (3%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXVNSGEKDDKMQSFFLAETLKYLYLLFSPPSVV--SFDEWVFNT 447
           + EK +++  GY  L  V     +D+M+SFFL+ET KYLYLLF   + V  S   ++F T
Sbjct: 520 SLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTT 579

Query: 446 EAH 438
           E H
Sbjct: 580 EGH 582



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>MNS1_CANAL (Q8J0Q0) Mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC|
           3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 565

 Score = 50.1 bits (118), Expect = 7e-06
 Identities = 22/38 (57%), Positives = 28/38 (73%)
 Frame = -1

Query: 548 DKMQSFFLAETLKYLYLLFSPPSVVSFDEWVFNTEAHP 435
           D  +SF+ AETLKYLYLLF   + +   ++VFNTEAHP
Sbjct: 468 DNTESFWWAETLKYLYLLFDDTNKIPLTDYVFNTEAHP 505



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>MNS1_YEAST (P32906) Endoplasmic reticulum mannosyl-oligosaccharide|
           1,2-alpha-mannosidase (EC 3.2.1.113) (ER
           alpha-1,2-mannosidase) (Man(9)-alpha-mannosidase)
          Length = 549

 Score = 49.3 bits (116), Expect = 1e-05
 Identities = 28/59 (47%), Positives = 38/59 (64%), Gaps = 2/59 (3%)
 Frame = -1

Query: 557 EKDDKMQSFFLAETLKYLYLLFSPPSVVSFD--EWVFNTEAHPLRIVPTHGSNGQSIET 387
           +K + M+SF+LAETLKYLY+LF    +  FD  + VFNTEAHP  ++       QS+ T
Sbjct: 491 KKSNNMESFWLAETLKYLYILF----LDEFDLTKVVFNTEAHPFPVLDEEILKSQSLTT 545



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>YKU1_SCHPO (Q9P7C3) Putative mannosyl-oligosaccharide 1,2-alpha-mannosidase|
           (EC 3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 521

 Score = 48.5 bits (114), Expect = 2e-05
 Identities = 23/42 (54%), Positives = 30/42 (71%), Gaps = 1/42 (2%)
 Frame = -1

Query: 557 EKDDKMQSFFLAETLKYLYLLFSPP-SVVSFDEWVFNTEAHP 435
           ++ DK +SF+ AETLKYLYLLF    S++    + FNTEAHP
Sbjct: 467 DQRDKTESFWFAETLKYLYLLFEDDFSILPLTNYTFNTEAHP 508



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>MAN12_PENCI (P31723) Mannosyl-oligosaccharide alpha-1,2-mannosidase precursor|
           (EC 3.2.1.113) (Man(9)-alpha-mannosidase)
          Length = 511

 Score = 43.1 bits (100), Expect = 8e-04
 Identities = 25/73 (34%), Positives = 35/73 (47%), Gaps = 8/73 (10%)
 Frame = -1

Query: 620 AFEKNSRIASGYVGLRXVNS---GEKDDKMQSFFLAETLKYLYLLFSPPSVVSFDE---- 462
           A     R  SG+  +  VN    G K D  +SF  AE +KY YL  S  +     +    
Sbjct: 436 AINSTCRTDSGFAAVSDVNKANGGSKYDNQESFLFAEVMKYSYLAHSEDAAWQVQKGGKN 495

Query: 461 -WVFNTEAHPLRI 426
            +V+NTEAHP+ +
Sbjct: 496 TFVYNTEAHPISV 508



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>Y1383_METJA (Q58778) Hypothetical protein MJ1383 precursor|
          Length = 256

 Score = 29.6 bits (65), Expect = 9.2
 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
 Frame = -1

Query: 662 IDPINNERREKVKXAFEKNSRIAS-GYVGLRXVNSGEKDDKMQSFFLAETLKYLYLLFSP 486
           I P  +E  +K+K     NSR+   G +GL      EK+  ++   LAE L    ++ +P
Sbjct: 75  IPPEIDEALDKIKSYINYNSRVVGIGEIGLEKATKEEKEVFIKQLLLAEELNMPAVVHTP 134


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,807,650
Number of Sequences: 219361
Number of extensions: 1766472
Number of successful extensions: 3578
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 3496
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3566
length of database: 80,573,946
effective HSP length: 109
effective length of database: 56,663,597
effective search space used: 6969622431
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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