ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd2d20
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1C27AA_BACUH (Q9S597) Pesticidal crystal protein cry27Aa (Insecti... 34 0.50
2SEC9_KLULA (Q6CSD1) Protein transport protein SEC9 33 0.85
3DNAA_STRCO (P27902) Chromosomal replication initiator protein dnaA 33 0.85
4RPB1_SCHPO (P36594) DNA-directed RNA polymerase II largest subun... 32 1.4
5YIIZ_SALTY (P43020) Hypothetical protein yiiZ precursor 32 1.9
6POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type... 31 4.2
7S18L1_HUMAN (O75177) SS18-like protein 1 (SYT homolog 1) 31 4.2
8CSKI2_HUMAN (Q8WXE0) Caskin-2 31 4.2
9DPOL_ADE05 (P04495) DNA polymerase (EC 2.7.7.7) 30 5.5
10DPOL_ADE02 (P03261) DNA polymerase (EC 2.7.7.7) 30 5.5
11CABP4_BOVIN (Q8HZJ4) Calcium-binding protein 4 (CaBP4) 30 7.2
12SOX18_HUMAN (P35713) Transcription factor SOX-18 30 7.2
13VP41_NPVOP (O10333) Structural glycoprotein gp41 30 7.2
14SYV_RHOPA (Q6N6N1) Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--t... 30 9.4
15IRS2_HUMAN (Q9Y4H2) Insulin receptor substrate 2 (IRS-2) 30 9.4

>C27AA_BACUH (Q9S597) Pesticidal crystal protein cry27Aa (Insecticidal|
            delta-endotoxin CryXXVIIA(a)) (Crystaline entomocidal
            protoxin) (94 kDa crystal protein)
          Length = 826

 Score = 33.9 bits (76), Expect = 0.50
 Identities = 28/135 (20%), Positives = 54/135 (40%), Gaps = 5/135 (3%)
 Frame = -3

Query: 571  MPNNVPNNASTPDYGMFS-DPYLFEQSEQYYDEEL----APYYSDMNGGPQGLRDRSYSQ 407
            +P  +P N ++  Y  +  +   + Q+   Y++ +     P Y +      G+ D  Y+Q
Sbjct: 680  IPVGIPLNQTSEGYNTYDQNTNSYNQNYNNYNQNMDTTYQPNYDNYKQNSSGMYDNPYNQ 739

Query: 406  FDLPSYSNSQFDDMVMGQWPYSRADNSMPNFGADINAYPYQSRDYQATSANAASRYPSYH 227
                SY+ +  D    G   Y+ + N   N+  + N Y   + +    S      Y +Y+
Sbjct: 740  NPKDSYNQNYTDTYDSG---YNNSQNVGSNYNQEYNTYNQDTENMYNQS------YNNYN 790

Query: 226  PGNGH*ALSEMNNDC 182
              N +      N+DC
Sbjct: 791  SDNNN---YNQNSDC 802



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>SEC9_KLULA (Q6CSD1) Protein transport protein SEC9|
          Length = 556

 Score = 33.1 bits (74), Expect = 0.85
 Identities = 35/142 (24%), Positives = 58/142 (40%), Gaps = 23/142 (16%)
 Frame = -3

Query: 580 QFSMPNNVPNNASTPDYGMFSDPYLFEQSEQYYDEELAPYYS--------------DMNG 443
           + SM NN   +AS    G + DPY  + +  Y + + +PY +                N 
Sbjct: 89  EVSMINNRNASASARHTGNYVDPYSNDNASPYGNSDSSPYGNASASPYDTGYNAPLQQNN 148

Query: 442 GPQGLRDRSYSQFDLPSY-SNSQFDDMVMGQWPYSRADNSMPNFG-------ADINAY-P 290
            P G  + SYS     SY SNS   D      P + + N+    G       A++N+  P
Sbjct: 149 NPYGSMNNSYSN---KSYGSNSYSGDTYASPSPQASSSNNNGATGQRPSNPYANMNSQDP 205

Query: 289 YQSRDYQATSANAASRYPSYHP 224
           Y +  Y  +++ + + Y  + P
Sbjct: 206 YGTNSY--SNSGSVNAYKGFTP 225



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>DNAA_STRCO (P27902) Chromosomal replication initiator protein dnaA|
          Length = 656

 Score = 33.1 bits (74), Expect = 0.85
 Identities = 35/147 (23%), Positives = 51/147 (34%), Gaps = 11/147 (7%)
 Frame = -3

Query: 619 SDRRKVLQPGFVRQFSMPN--NVPNNASTPDYGMFS--------DPYLFEQSEQYYDEEL 470
           +D+    +P + +++  P   + P  A   DYG           DPY     E Y  E  
Sbjct: 144 ADQLPTARPAYPQEYQRPEPGSWPRPAQQDDYGWQQQRLGFPERDPYASPNQEPYGQEPP 203

Query: 469 APYYSDMNGGPQGLRDRSYSQFDLPSYSNSQFD-DMVMGQWPYSRADNSMPNFGADINAY 293
            PY  +     Q  +D      + PSY   + D +   G++   R D   P        Y
Sbjct: 204 PPYSHENRTSYQ--QDYRPQPPERPSYDAQRGDYEQARGEYEQPRGDYDKPR-----GDY 256

Query: 292 PYQSRDYQATSANAASRYPSYHPGNGH 212
             Q  DY           P   PG+GH
Sbjct: 257 DQQRGDYD--QRGPRRDLPEPPPGSGH 281



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>RPB1_SCHPO (P36594) DNA-directed RNA polymerase II largest subunit (EC 2.7.7.6)|
            (RNA polymerase II subunit 1)
          Length = 1752

 Score = 32.3 bits (72), Expect = 1.4
 Identities = 37/111 (33%), Positives = 47/111 (42%), Gaps = 7/111 (6%)
 Frame = -3

Query: 535  DYGMF--SDPYLFEQSEQYYDEELAPYYSDMNGGPQGLRDRSYSQFDLPSYSNSQFDDMV 362
            DYG+   + PY   QS  Y     +P+ S M+ G  GL   SYS    P YS S      
Sbjct: 1521 DYGLLGAASPYKGVQSPGY----TSPFSSAMSPG-YGLTSPSYSPSS-PGYSTSP---AY 1571

Query: 361  MGQWP-YSRADNSM----PNFGADINAYPYQSRDYQATSANAASRYPSYHP 224
            M   P YS    S     P++     +Y   S  Y ATS + +   PSY P
Sbjct: 1572 MPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSP 1622



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>YIIZ_SALTY (P43020) Hypothetical protein yiiZ precursor|
          Length = 327

 Score = 32.0 bits (71), Expect = 1.9
 Identities = 12/20 (60%), Positives = 16/20 (80%)
 Frame = -3

Query: 541 TPDYGMFSDPYLFEQSEQYY 482
           +P+YG+FS PYLF   E+YY
Sbjct: 97  SPEYGVFSLPYLFATVEEYY 116



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>POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type-2|
            retrotransposable element R2DM (Retrovirus-related Pol
            polyprotein from type II retrotransposable element R2DM)
            [Includes: Protease (EC 3.4.23.-); Reverse transcriptase
            (EC 2.7.7.49); End
          Length = 1057

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 24/90 (26%), Positives = 35/90 (38%)
 Frame = +1

Query: 154  GLQHRIYYGCNHYSSLKGLNAHSQGDRMDSGLLRSLRSPGNPWTDRGRH*CLPQSLAWSC 333
            GL+   +YG  H    +     +  + MD   LR    P    T RGRH      L   C
Sbjct: 827  GLREGGHYGPQHGWVSQPTRLLTGKEYMDGIRLRINALPTKSRTTRGRH-----ELERQC 881

Query: 334  RRGCMATGPSPYHQIGCWSMRGDQTVNNSC 423
            R GC A   + +    C+   G +   ++C
Sbjct: 882  RAGCDAPETTNHIMQKCYRSHGRRVARHNC 911



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>S18L1_HUMAN (O75177) SS18-like protein 1 (SYT homolog 1)|
          Length = 396

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 25/97 (25%), Positives = 40/97 (41%), Gaps = 4/97 (4%)
 Frame = -3

Query: 493 EQYYDEELAPYYSDMNGGPQGLRDRSYSQFDLPSYS--NSQFDDMVMG--QWPYSRADNS 326
           +QYY +    Y    +   +   DRS+ +     Y   NSQ+     G  Q    +   S
Sbjct: 269 QQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYS 328

Query: 325 MPNFGADINAYPYQSRDYQATSANAASRYPSYHPGNG 215
              + +   +YP Q + Y  ++  A S+YP Y  G G
Sbjct: 329 QQQYPSQ-QSYPGQQQGY-GSAQGAPSQYPGYQQGQG 363



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>CSKI2_HUMAN (Q8WXE0) Caskin-2|
          Length = 1202

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = -1

Query: 303 SMPTPISPGITRRPQRTQQAAIHPIT 226
           S PTP+ PG +R PQ   +   HP+T
Sbjct: 358 SAPTPLRPGFSRTPQPPAEEPPHPLT 383



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>DPOL_ADE05 (P04495) DNA polymerase (EC 2.7.7.7)|
          Length = 1056

 Score = 30.4 bits (67), Expect = 5.5
 Identities = 18/68 (26%), Positives = 30/68 (44%)
 Frame = -3

Query: 433 GLRDRSYSQFDLPSYSNSQFDDMVMGQWPYSRADNSMPNFGADINAYPYQSRDYQATSAN 254
           GL D S++ F  P+ S++     +  Q  +     +  N G D+ A  ++  DY   S  
Sbjct: 466 GLTDASFNVFQRPTISSNSH--AIFRQIVFRAEQPARSNLGPDLLAPSHELYDYVRASIR 523

Query: 253 AASRYPSY 230
               YP+Y
Sbjct: 524 GGRCYPTY 531



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>DPOL_ADE02 (P03261) DNA polymerase (EC 2.7.7.7)|
          Length = 1056

 Score = 30.4 bits (67), Expect = 5.5
 Identities = 18/68 (26%), Positives = 30/68 (44%)
 Frame = -3

Query: 433 GLRDRSYSQFDLPSYSNSQFDDMVMGQWPYSRADNSMPNFGADINAYPYQSRDYQATSAN 254
           GL D S++ F  P+ S++     +  Q  +     +  N G D+ A  ++  DY   S  
Sbjct: 466 GLTDASFNVFQRPTISSNSH--AIFRQIVFRAEQPARSNLGPDLLAPSHELYDYVRASIR 523

Query: 253 AASRYPSY 230
               YP+Y
Sbjct: 524 GGRCYPTY 531



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>CABP4_BOVIN (Q8HZJ4) Calcium-binding protein 4 (CaBP4)|
          Length = 279

 Score = 30.0 bits (66), Expect = 7.2
 Identities = 14/41 (34%), Positives = 21/41 (51%)
 Frame = -1

Query: 327 PCQTLGQTSMPTPISPGITRRPQRTQQAAIHPITLGMGIEP 205
           P  +  QT M  P +PG ++ P RT++    PI    G+ P
Sbjct: 56  PSSSGEQTPMQGPEAPGSSKNPSRTREGQEGPIPSASGLAP 96



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>SOX18_HUMAN (P35713) Transcription factor SOX-18|
          Length = 384

 Score = 30.0 bits (66), Expect = 7.2
 Identities = 15/48 (31%), Positives = 21/48 (43%)
 Frame = -1

Query: 360 WASGHTAAPTTPCQTLGQTSMPTPISPGITRRPQRTQQAAIHPITLGM 217
           WA GH AA  T     G  ++  P +P     PQR+   +  P   G+
Sbjct: 22  WAPGHGAAADTRGLAAGPAALAAPAAPASPPSPQRSPPRSPEPGRYGL 69



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>VP41_NPVOP (O10333) Structural glycoprotein gp41|
          Length = 367

 Score = 30.0 bits (66), Expect = 7.2
 Identities = 14/37 (37%), Positives = 20/37 (54%)
 Frame = -1

Query: 354 SGHTAAPTTPCQTLGQTSMPTPISPGITRRPQRTQQA 244
           SGH  APT+   T  Q+  PT +S  ++R   R+  A
Sbjct: 14  SGHPFAPTSATLTSSQSGYPTAVSTTLSRADSRSNTA 50



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>SYV_RHOPA (Q6N6N1) Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--tRNA ligase)|
           (ValRS)
          Length = 957

 Score = 29.6 bits (65), Expect = 9.4
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = -2

Query: 365 GDGPVAIQPRRQLHAKLWGRHQCLPLSVQGLPGDLSERSKP 243
           GD  VA+ P  + +  L G+H  LPL  + +P    E S P
Sbjct: 233 GDSAVAVNPEDERYTHLVGKHVILPLVGRRIPIVADEYSDP 273



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>IRS2_HUMAN (Q9Y4H2) Insulin receptor substrate 2 (IRS-2)|
          Length = 1338

 Score = 29.6 bits (65), Expect = 9.4
 Identities = 13/45 (28%), Positives = 23/45 (51%), Gaps = 6/45 (13%)
 Frame = -1

Query: 321  QTLGQTSMPTPISPGITRRPQRTQQAAIHPITL------GMGIEP 205
            +T   T+  TP+SP     P+R   A++  ++L      G+G+ P
Sbjct: 1150 ETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGP 1194


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,845,251
Number of Sequences: 219361
Number of extensions: 2236741
Number of successful extensions: 5908
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 5674
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5903
length of database: 80,573,946
effective HSP length: 109
effective length of database: 56,663,597
effective search space used: 7082949625
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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