ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbasd1j15
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1CHAC_ECOLI (P39163) Cation transport protein chaC 40 0.002
2VG20_BPT4 (P13334) Structural protein of head (Tail tube protein... 33 0.49
3DCE2_HUMAN (Q05329) Glutamate decarboxylase 2 (EC 4.1.1.15) (Glu... 30 2.4
4YEZ3_YEAST (P32656) Hypothetical 26.3 kDa protein in RAD4-CHD1 i... 30 2.4
5DOCK1_MOUSE (Q8BUR4) Dedicator of cytokinesis protein 1 (180 kDa... 30 4.1
6MRC1_SCHPO (Q9P7T4) Mediator of replication checkpoint protein 1... 29 5.4
7DCE2_RAT (Q05683) Glutamate decarboxylase 2 (EC 4.1.1.15) (Gluta... 29 5.4
8DCE2_PIG (P48321) Glutamate decarboxylase 2 (EC 4.1.1.15) (Gluta... 29 5.4
9DCE2_MOUSE (P48320) Glutamate decarboxylase 2 (EC 4.1.1.15) (Glu... 29 5.4
10DCE2_CANFA (Q4PRC2) Glutamate decarboxylase 2 (EC 4.1.1.15) (Glu... 29 5.4
11VATI1_TREPA (O83444) V-type ATP synthase subunit I 1 (EC 3.6.3.1... 29 5.4
12PGK_HELHP (Q7VJB6) Phosphoglycerate kinase (EC 2.7.2.3) 29 7.1
13TRMD_CHLPN (Q9Z964) tRNA (guanine-N(1)-)-methyltransferase (EC 2... 28 9.2
14COAE_TREPA (O83319) Dephospho-CoA kinase (EC 2.7.1.24) (Dephosph... 28 9.2

>CHAC_ECOLI (P39163) Cation transport protein chaC|
          Length = 231

 Score = 40.4 bits (93), Expect = 0.002
 Identities = 21/50 (42%), Positives = 30/50 (60%)
 Frame = -1

Query: 460 MARQIATASGPNGYNRDYLFSMEKALSNISHEDDSIIVLADEVRKVLGRS 311
           +A  IA ASGP G N  YLFS+E+ L  +  +DD +  L   V+K+L  +
Sbjct: 171 IAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLAEN 220



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>VG20_BPT4 (P13334) Structural protein of head (Tail tube protein Gp20)|
          Length = 524

 Score = 32.7 bits (73), Expect = 0.49
 Identities = 13/36 (36%), Positives = 22/36 (61%)
 Frame = +2

Query: 278 WYVGAGNLLLCRPAQHLPHLIRKHNDRVVFMAYIGQ 385
           WYV  GN+   + A+H+ H++    +RVV+ A  G+
Sbjct: 285 WYVDTGNMPARKAAEHMQHVMNTMKNRVVYDASTGK 320



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>DCE2_HUMAN (Q05329) Glutamate decarboxylase 2 (EC 4.1.1.15) (Glutamate|
           decarboxylase, 65 kDa isoform) (GAD-65) (65 kDa glutamic
           acid decarboxylase)
          Length = 585

 Score = 30.4 bits (67), Expect = 2.4
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = -2

Query: 219 FQHSKKSGMAGLAKQMAFSSDHTHS*HSPIERSAVYFGSGT 97
           F   K+ GMA L + +AF+S+H+   H  +++ A   G GT
Sbjct: 259 FPEVKEKGMAALPRLIAFTSEHS---HFSLKKGAAALGIGT 296



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>YEZ3_YEAST (P32656) Hypothetical 26.3 kDa protein in RAD4-CHD1 intergenic|
           region
          Length = 232

 Score = 30.4 bits (67), Expect = 2.4
 Identities = 12/32 (37%), Positives = 23/32 (71%)
 Frame = -1

Query: 469 LEDMARQIATASGPNGYNRDYLFSMEKALSNI 374
           +++ A+ IA + GP+G N +YL  +E+AL+ +
Sbjct: 176 VDETAKVIAVSHGPSGSNYEYLAKLEQALAQM 207



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>DOCK1_MOUSE (Q8BUR4) Dedicator of cytokinesis protein 1 (180 kDa protein|
           downstream of CRK) (DOCK180) (Fragments)
          Length = 1027

 Score = 29.6 bits (65), Expect = 4.1
 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
 Frame = +3

Query: 135 ENVKSEYGQRKMPS-VSLIQPCRSSCYVETATVPSGRAEMCTAGVCDFNGTSEP 293
           E V+ +YG R MPS +   +  R    V + T+PS    +  A V  F+  S P
Sbjct: 768 EKVEKQYGVRTMPSGLDDRRGSRPRSMVRSFTMPSSSRPLSVASVSSFSSDSTP 821



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>MRC1_SCHPO (Q9P7T4) Mediator of replication checkpoint protein 1 (DNA|
            replication checkpoint mediator mrc1)
          Length = 1019

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 19/85 (22%), Positives = 43/85 (50%), Gaps = 5/85 (5%)
 Frame = -1

Query: 469  LEDMARQIATASGPNGYNRDYLFSMEKALSNISHEDDSIIVLADEVRKVLGRSTEKKV-- 296
            +E+   +I ++ GPN  +R YL       ++ ++  + ++ +   VRK++  S  +K+  
Sbjct: 886  MEEELSEIFSSGGPNVVDRVYLKKSSTRHTSDNNSLEEVLPIFPGVRKLVSNSQSEKIGD 945

Query: 295  --TGSDVPLKSH-TPAVHISALPEG 230
                + +  KS+ TP +  +  P+G
Sbjct: 946  LSNDNSMGAKSYKTPIISSTQRPQG 970



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>DCE2_RAT (Q05683) Glutamate decarboxylase 2 (EC 4.1.1.15) (Glutamate|
           decarboxylase, 65 kDa isoform) (GAD-65) (65 kDa glutamic
           acid decarboxylase)
          Length = 585

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 14/41 (34%), Positives = 24/41 (58%)
 Frame = -2

Query: 219 FQHSKKSGMAGLAKQMAFSSDHTHS*HSPIERSAVYFGSGT 97
           F   K+ GMA + + +AF+S+H+   H  +++ A   G GT
Sbjct: 259 FPEVKEKGMAAVPRLIAFTSEHS---HFSLKKGAAALGIGT 296



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>DCE2_PIG (P48321) Glutamate decarboxylase 2 (EC 4.1.1.15) (Glutamate|
           decarboxylase, 65 kDa isoform) (GAD-65) (65 kDa glutamic
           acid decarboxylase)
          Length = 585

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 14/41 (34%), Positives = 24/41 (58%)
 Frame = -2

Query: 219 FQHSKKSGMAGLAKQMAFSSDHTHS*HSPIERSAVYFGSGT 97
           F   K+ GMA + + +AF+S+H+   H  +++ A   G GT
Sbjct: 259 FPEVKEKGMAAVPRLIAFTSEHS---HFSLKKGAAALGIGT 296



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>DCE2_MOUSE (P48320) Glutamate decarboxylase 2 (EC 4.1.1.15) (Glutamate|
           decarboxylase, 65 kDa isoform) (GAD-65) (65 kDa glutamic
           acid decarboxylase)
          Length = 585

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 14/41 (34%), Positives = 24/41 (58%)
 Frame = -2

Query: 219 FQHSKKSGMAGLAKQMAFSSDHTHS*HSPIERSAVYFGSGT 97
           F   K+ GMA + + +AF+S+H+   H  +++ A   G GT
Sbjct: 259 FPEVKEKGMAAVPRLIAFTSEHS---HFSLKKGAAALGIGT 296



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>DCE2_CANFA (Q4PRC2) Glutamate decarboxylase 2 (EC 4.1.1.15) (Glutamate|
           decarboxylase, 65 kDa isoform) (GAD-65) (65 kDa glutamic
           acid decarboxylase)
          Length = 585

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 14/41 (34%), Positives = 24/41 (58%)
 Frame = -2

Query: 219 FQHSKKSGMAGLAKQMAFSSDHTHS*HSPIERSAVYFGSGT 97
           F   K+ GMA + + +AF+S+H+   H  +++ A   G GT
Sbjct: 259 FPEVKEKGMAAVPRLIAFTSEHS---HFSLKKGAAALGIGT 296



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>VATI1_TREPA (O83444) V-type ATP synthase subunit I 1 (EC 3.6.3.14) (V-type|
           ATPase subunit I 1)
          Length = 622

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = +2

Query: 317 AQHLPHLIRKHNDRVVFMAYIGQCLLH*EQVVP 415
           A+ +  ++R H+DRV     I QC+ H E+  P
Sbjct: 83  AEAVEQIVRTHSDRVELAQRIAQCIAHLERCEP 115



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>PGK_HELHP (Q7VJB6) Phosphoglycerate kinase (EC 2.7.2.3)|
          Length = 402

 Score = 28.9 bits (63), Expect = 7.1
 Identities = 23/71 (32%), Positives = 32/71 (45%), Gaps = 9/71 (12%)
 Frame = -1

Query: 403 FSMEKALSNISHEDDSIIVLADEVRKVLGRSTEKK---------VTGSDVPLKSHTPAVH 251
           F M+K+L     EDD    L  E RK+L ++ EKK         V+  D+    H     
Sbjct: 240 FDMQKSLV----EDD----LVSEARKILDKAKEKKVKIYLPVDVVSTDDIKEHRHIKITP 291

Query: 250 ISALPEGTVAV 218
              +PEG +AV
Sbjct: 292 AQDIPEGFMAV 302



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>TRMD_CHLPN (Q9Z964) tRNA (guanine-N(1)-)-methyltransferase (EC 2.1.1.31)|
           (M1G-methyltransferase) (tRNA [GM37] methyltransferase)
          Length = 361

 Score = 28.5 bits (62), Expect = 9.2
 Identities = 17/57 (29%), Positives = 26/57 (45%)
 Frame = +2

Query: 221 RDGALGKSRDVHSWSMRF*WYVGAGNLLLCRPAQHLPHLIRKHNDRVVFMAYIGQCL 391
           RD  LGK + V         + G G LL+  P       +RK N +V++++  G  L
Sbjct: 39  RDFGLGKWKQVDDTP-----FSGGGMLLMAEPVTSAIRSVRKENSKVIYLSPQGALL 90



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>COAE_TREPA (O83319) Dephospho-CoA kinase (EC 2.7.1.24) (Dephosphocoenzyme A|
           kinase)
          Length = 211

 Score = 28.5 bits (62), Expect = 9.2
 Identities = 20/49 (40%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
 Frame = -2

Query: 366 KTTRSLCLRMR*GRCWAGRQRRR-LPAPTYH*NRILQLCTSLLFPRAPS 223
           K TR LC  +   R  A R +   L APT H   +LQ C+ +L+  APS
Sbjct: 98  KVTRLLCEDI--ARAQAARPKAIVLNAPTLHKTELLQACSFVLYIWAPS 144


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,233,105
Number of Sequences: 219361
Number of extensions: 1288494
Number of successful extensions: 3085
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 3039
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3085
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3130907202
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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