ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbags9n15 |
|---|---|
| Clone Library Name | barley_pub |
>GSH1_ARATH (P46309) Glutamate--cysteine ligase, chloroplast precursor (EC| 6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS) Length = 522 Score = 143 bits (361), Expect = 4e-34 Identities = 70/90 (77%), Positives = 78/90 (86%) Frame = -2 Query: 624 TKEEREMLRRKVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVR 445 T EREMLR KVPVTGLKTPFRDG ++ +AEDVL+LAK+GLERRGYKE GFL VDEVVR Sbjct: 433 TPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDEVVR 492 Query: 444 TGVTPAEKLLNLYETKWQRSVDPVFEELLY 355 TGVTPAEKLL +Y +W +SVDPVFEELLY Sbjct: 493 TGVTPAEKLLEMYNGEWGQSVDPVFEELLY 522
>GSH1_BRAJU (O23736) Glutamate--cysteine ligase, chloroplast precursor (EC| 6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS) Length = 514 Score = 139 bits (350), Expect = 7e-33 Identities = 68/90 (75%), Positives = 77/90 (85%) Frame = -2 Query: 624 TKEEREMLRRKVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVR 445 T EREMLR KVPVTGLKTPFRDG ++ +AEDVL+LAK+GLERRGYKEVGFL V EVVR Sbjct: 425 TPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEVGFLNAVTEVVR 484 Query: 444 TGVTPAEKLLNLYETKWQRSVDPVFEELLY 355 TGVTPAE LL +Y +W +SVDPVF+ELLY Sbjct: 485 TGVTPAENLLEMYNGEWGQSVDPVFQELLY 514
>GSH1_LYCES (O22493) Glutamate--cysteine ligase, chloroplast precursor (EC| 6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS) Length = 523 Score = 135 bits (340), Expect = 1e-31 Identities = 65/90 (72%), Positives = 76/90 (84%) Frame = -2 Query: 624 TKEEREMLRRKVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVR 445 T EER+MLR KVP +GLKTPFRDG + +A+DV++LAK GLERRG+KE GFL EV EVV+ Sbjct: 434 TAEERDMLRNKVPKSGLKTPFRDGLLMHVAQDVVKLAKEGLERRGFKETGFLNEVAEVVK 493 Query: 444 TGVTPAEKLLNLYETKWQRSVDPVFEELLY 355 TGVTPAEKLL LY KW +SVDP+FEELLY Sbjct: 494 TGVTPAEKLLELYHGKWGQSVDPIFEELLY 523
>GSH1_MEDTR (Q9ZNX6) Glutamate--cysteine ligase, chloroplast precursor (EC| 6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS) Length = 508 Score = 134 bits (336), Expect = 3e-31 Identities = 66/90 (73%), Positives = 77/90 (85%) Frame = -2 Query: 624 TKEEREMLRRKVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVR 445 T EEREMLR KV VTGLKTPFRDG ++ +AE+VL+LAK+GLERRG+KE GFL V EVVR Sbjct: 419 TLEEREMLRNKVTVTGLKTPFRDGLLKHVAEEVLELAKDGLERRGFKESGFLNAVAEVVR 478 Query: 444 TGVTPAEKLLNLYETKWQRSVDPVFEELLY 355 TGVTPAE+LL LY KW++SVD VF+ELLY Sbjct: 479 TGVTPAERLLELYHGKWEQSVDHVFDELLY 508
>NUDH_ZYMMO (Q9RH11) Probable (di)nucleoside polyphosphate hydrolase (EC| 3.6.1.-) Length = 155 Score = 33.9 bits (76), Expect = 0.40 Identities = 17/50 (34%), Positives = 28/50 (56%) Frame = -2 Query: 543 DLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNLYETKW 394 D+ E+ Q+ + GLE + EVG LRE++E TG+ P + + +W Sbjct: 28 DMKEEAWQMPQGGLEAKETPEVGVLRELEE--ETGIPPRMVAIISHTKEW 75
>ACDS_MEGEL (Q06319) Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)| (SCAD) (Butyryl-CoA dehydrogenase) (BCAD) Length = 383 Score = 31.6 bits (70), Expect = 2.0 Identities = 30/117 (25%), Positives = 50/117 (42%), Gaps = 8/117 (6%) Frame = -2 Query: 558 DGYVRDLAEDVLQLAKNGLER--------RGYKEVGFLREVDEVVRTGVTPAEKLLNLYE 403 D + D+ +D L+LA + E+ R +K + +DE++ G+T A +E Sbjct: 2 DFNLTDIQQDFLKLAHDFGEKKLAPTVTERDHKGIYDKELIDELLSLGITGA-----YFE 56 Query: 402 TKWQRSVDPVFEELLY*YTESHITSVDAGQGGQGLQTVSLYKNPVLNSTSHLTRETY 232 K+ S D + L Y + DAG TVSL NP+ + +E + Sbjct: 57 EKYGGSGDDGGDVLSYILAVEELAKYDAGVAITLSATVSLCANPIWQFGTEAQKEKF 113
>TYCC_BREPA (O30409) Tyrocidine synthetase 3 (Tyrocidine synthetase III)| [Includes: ATP-dependent asparagine adenylase (AsnA) (Asparagine activase); ATP-dependent glutamine adenylase (GlnA) (Glutamine activase); ATP-dependent tyrosine adenylase (TyrA) (Ty Length = 6486 Score = 31.2 bits (69), Expect = 2.6 Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 4/64 (6%) Frame = +1 Query: 157 TGERKTASH---KQSVPSSKLFAAARPEVGFPRQVACGIEDGIF-VQGYSLKTLSTLSSV 324 +GER T S Q+ +KL + +G P A GI D F + G+SLK ++ ++ V Sbjct: 4064 SGERTTGSAFVAAQNDTEAKLQQIWQEVLGIP---AIGIHDNFFEIGGHSLKAMNVITQV 4120 Query: 325 HRSY 336 H+++ Sbjct: 4121 HKTF 4124
>CCNB3_CANFA (Q659K0) G2/mitotic-specific cyclin-B3| Length = 1330 Score = 30.8 bits (68), Expect = 3.4 Identities = 13/29 (44%), Positives = 14/29 (48%) Frame = +2 Query: 269 TGFLYRDTV*RPCPPCPASTEVICDSVYQ 355 T FL PCPPC ICD +YQ Sbjct: 1152 TAFLIAAKFEEPCPPCVDDFLYICDDIYQ 1180
>RSSA_CAEEL (P46769) Probable 40S ribosomal protein SA (p40)| Length = 275 Score = 30.4 bits (67), Expect = 4.4 Identities = 19/55 (34%), Positives = 26/55 (47%) Frame = +2 Query: 62 QGKVKKGSVQTGVCSGSLEMREKS*VVKDPPQLEKEKQQATNRVFRAQNYSQPRD 226 +GK+ + QTG E+ +DP + EKE+ A V AQ Y QP D Sbjct: 185 RGKISR---QTGFVLEGKEIMPDLYFYRDPTETEKEETGAHADVAEAQEYQQPTD 236
>TKT1_KLULA (Q12630) Transketolase (EC 2.2.1.1) (TK)| Length = 679 Score = 30.0 bits (66), Expect = 5.8 Identities = 18/54 (33%), Positives = 30/54 (55%) Frame = -1 Query: 226 VSRLRIILSSEHSVCGLLFFFLQLWGIFHNLRFFSHFQGAGTNPCLYRPLFHLP 65 ++R R +LS+ H+ C LL+ L L+G ++ HF+ G+ + P F LP Sbjct: 58 INRDRFVLSNGHA-CALLYSLLHLFGYDMSIEDLKHFRHLGSKTPGH-PEFELP 109
>IKAR_MOUSE (Q03267) DNA-binding protein Ikaros (Lymphoid transcription factor| LyF-1) Length = 517 Score = 30.0 bits (66), Expect = 5.8 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = -1 Query: 304 GSSDCIPVQKSRPQFHKPPDEG 239 GSS+ +PV S Q HKPP +G Sbjct: 332 GSSEVVPVISSMYQLHKPPSDG 353
>VG29_BPMU (Q9T1W5) Protein gp29| Length = 512 Score = 29.6 bits (65), Expect = 7.6 Identities = 14/52 (26%), Positives = 27/52 (51%) Frame = -2 Query: 510 NGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNLYETKWQRSVDPVFEELLY 355 NG ++ + + D++ R GV+P + WQRSVDP+ + +++ Sbjct: 421 NGSQKEAALSAEDIPQEDDIDRMGVSPED---------WQRSVDPLLKPVIF 463
>U520_CAEEL (Q9U2G0) Putative U5 small nuclear ribonucleoprotein 200 kDa helicase| (EC 3.6.1.-) Length = 2145 Score = 29.6 bits (65), Expect = 7.6 Identities = 19/80 (23%), Positives = 31/80 (38%), Gaps = 3/80 (3%) Frame = -2 Query: 534 EDVLQLAKNGLERRGYKEVGFLREVD---EVVRTGVTPAEKLLNLYETKWQRSVDPVFEE 364 E+V+ A NG + E+ LR + E +TP E + W+R ++P Sbjct: 1338 ENVIVCAPNGSGKTAIAELAVLRHFENTPEAKAVYITPMEDMATKVYADWKRRLEPAIGH 1397 Query: 363 LLY*YTESHITSVDAGQGGQ 304 + T + Q GQ Sbjct: 1398 TIVLLTGEQTMDLKLAQRGQ 1417
>SYFB_PSEPK (Q88K22) Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)| (Phenylalanine--tRNA ligase beta chain) (PheRS) Length = 793 Score = 29.3 bits (64), Expect = 9.9 Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 2/68 (2%) Frame = -3 Query: 431 LLRSF*IYTRRSGSAAWTPSSRSCYIDTLNHI*LRWTLDKVDRVFRLYPCTKIPSSIPQA 252 LL + + T +SG WT S S D + +D ++ + RLY +P PQA Sbjct: 429 LLNALELTTTKSGEGQWTVSVPSHRFD------ISLEVDLIEELARLYGYNNLPVRYPQA 482 Query: 251 --T*RGKP 234 +GKP Sbjct: 483 RLAPQGKP 490
>YDCS_ECOLI (P76108) Putative ABC transporter periplasmic-binding protein ydcS| precursor Length = 381 Score = 29.3 bits (64), Expect = 9.9 Identities = 24/75 (32%), Positives = 35/75 (46%), Gaps = 9/75 (12%) Frame = +2 Query: 11 PSSHDXXMDLFRIQAVHQGKVKKGSVQTGVCSGSLEMREKS*VVK---------DPPQLE 163 P+ D +F Q + GK KG VQ G + + + + VK DP QL Sbjct: 157 PTPPDSWQVVFVEQNLPDGKSNKGRVQA--YDGPIYIADAALFVKATQPQLGISDPYQLT 214 Query: 164 KEKQQATNRVFRAQN 208 +E+ QA +V RAQ+ Sbjct: 215 EEQYQAVLKVLRAQH 229 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 95,433,947 Number of Sequences: 219361 Number of extensions: 2105670 Number of successful extensions: 6325 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 6103 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 6323 length of database: 80,573,946 effective HSP length: 107 effective length of database: 57,102,319 effective search space used: 5710231900 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
