ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbags9g12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1ILV5_PEA (O82043) Ketol-acid reductoisomerase, chloroplast precu... 306 4e-83
2ILV5_SPIOL (Q01292) Ketol-acid reductoisomerase, chloroplast pre... 305 1e-82
3ILV5_ARATH (Q05758) Ketol-acid reductoisomerase, chloroplast pre... 304 1e-82
4ILV5_NEUCR (P38674) Ketol-acid reductoisomerase, mitochondrial p... 68 3e-11
5ILV5_SCHPO (P78827) Probable ketol-acid reductoisomerase, mitoch... 62 1e-09
6ILV5_YEAST (P06168) Ketol-acid reductoisomerase, mitochondrial p... 62 2e-09
7NFRB_ECOLI (P0AFA5) Bacteriophage N4 adsorption protein B 32 2.3
8NFRB_ECO57 (P0AFA6) Bacteriophage N4 adsorption protein B 32 2.3
9SYS_COREF (Q8FLY5) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--... 31 3.8
10ICAA_STAAW (Q8NUI7) Biofilm PIA synthesis N-glycosyltransferase ... 30 5.0
11ICAA_STAAU (Q9RQP9) Biofilm PIA synthesis N-glycosyltransferase ... 30 5.0
12ICAA_STAAS (Q6G608) Biofilm PIA synthesis N-glycosyltransferase ... 30 5.0
13ICAA_STAAR (Q6GDD8) Biofilm PIA synthesis N-glycosyltransferase ... 30 5.0
14ICAA_STAAN (Q7A351) Biofilm PIA synthesis N-glycosyltransferase ... 30 5.0
15ICAA_STAAM (Q99QX3) Biofilm PIA synthesis N-glycosyltransferase ... 30 5.0
16ICAA_STAAC (Q5HCN1) Biofilm PIA synthesis N-glycosyltransferase ... 30 5.0
17ILVC_DEHE1 (Q3Z891) Ketol-acid reductoisomerase (EC 1.1.1.86) (A... 30 6.6

>ILV5_PEA (O82043) Ketol-acid reductoisomerase, chloroplast precursor (EC|
           1.1.1.86) (Acetohydroxy-acid reductoisomerase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 581

 Score =  306 bits (784), Expect = 4e-83
 Identities = 150/165 (90%), Positives = 158/165 (95%)
 Frame = -1

Query: 669 AFPMGKIDQTRMWKVGEKVRSTRPDGDLGPLHPFTAGVYVALMMAQIEVLRKKGHSYSEI 490
           AFPMGKIDQTRMWKVGE+VRSTRP GDLGPL+PFTAGV+VA+MMAQIEVLRKKGHSYSEI
Sbjct: 414 AFPMGKIDQTRMWKVGERVRSTRPAGDLGPLYPFTAGVFVAMMMAQIEVLRKKGHSYSEI 473

Query: 489 INESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILTQQAFVTVDKDAP 310
           INESVIESVDSLNPFMHARGV+FMVDNCSTTARLGSRKWAPRFDYILTQQA V VD  AP
Sbjct: 474 INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAP 533

Query: 309 INQDLISNFMSDPVHGAIEVCAELRPTVDISVTADADFVRPELRQ 175
           INQDLISNF+SDPVHGAI+VCAELRPT+DISV A ADFVRPELRQ
Sbjct: 534 INQDLISNFVSDPVHGAIQVCAELRPTLDISVPAAADFVRPELRQ 578



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>ILV5_SPIOL (Q01292) Ketol-acid reductoisomerase, chloroplast precursor (EC|
           1.1.1.86) (Acetohydroxy-acid reductoisomerase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 595

 Score =  305 bits (780), Expect = 1e-82
 Identities = 149/166 (89%), Positives = 158/166 (95%)
 Frame = -1

Query: 669 AFPMGKIDQTRMWKVGEKVRSTRPDGDLGPLHPFTAGVYVALMMAQIEVLRKKGHSYSEI 490
           AFPMGKIDQTRMWKVGEKVRS RP GDLGPL+PFTAGVYVALMMAQIE+LRKKGHSYSEI
Sbjct: 430 AFPMGKIDQTRMWKVGEKVRSVRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEI 489

Query: 489 INESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILTQQAFVTVDKDAP 310
           INESVIE+VDSLNPFMHARGV+FMVDNCSTTARLGSRKWAPRFDYIL+QQA V VD  AP
Sbjct: 490 INESVIEAVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILSQQALVAVDNGAP 549

Query: 309 INQDLISNFMSDPVHGAIEVCAELRPTVDISVTADADFVRPELRQS 172
           INQDLISNF+SDPVH AI VCA+LRP+VDISVTADADFVRPELRQ+
Sbjct: 550 INQDLISNFLSDPVHEAIGVCAQLRPSVDISVTADADFVRPELRQA 595



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>ILV5_ARATH (Q05758) Ketol-acid reductoisomerase, chloroplast precursor (EC|
           1.1.1.86) (Acetohydroxy-acid reductoisomerase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 591

 Score =  304 bits (779), Expect = 1e-82
 Identities = 149/167 (89%), Positives = 157/167 (94%)
 Frame = -1

Query: 669 AFPMGKIDQTRMWKVGEKVRSTRPDGDLGPLHPFTAGVYVALMMAQIEVLRKKGHSYSEI 490
           AFPMG IDQTRMWKVGE+VR +RP GDLGPL+PFTAGVYVALMMAQIE+LRKKGHSYSEI
Sbjct: 424 AFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEI 483

Query: 489 INESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILTQQAFVTVDKDAP 310
           INESVIESVDSLNPFMHARGV+FMVDNCSTTARLGSRKWAPRFDYILTQQA V VD  A 
Sbjct: 484 INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAA 543

Query: 309 INQDLISNFMSDPVHGAIEVCAELRPTVDISVTADADFVRPELRQSA 169
           IN+DLISNF SDPVHGAIEVCA+LRPTVDISV ADADFVRPELRQS+
Sbjct: 544 INRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSS 590



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>ILV5_NEUCR (P38674) Ketol-acid reductoisomerase, mitochondrial precursor (EC|
           1.1.1.86) (Acetohydroxy-acid reductoisomerase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 402

 Score = 67.8 bits (164), Expect = 3e-11
 Identities = 32/62 (51%), Positives = 43/62 (69%)
 Frame = -1

Query: 546 LMMAQIEVLRKKGHSYSEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAP 367
           + +AQ EVLR++GHS SE  NE+V E+  SL P + A G+ +M D CSTTAR G+  W P
Sbjct: 276 MFLAQYEVLRERGHSPSEAFNETVEEATQSLYPLIGAHGMDWMFDACSTTARRGAIDWTP 335

Query: 366 RF 361
           +F
Sbjct: 336 KF 337



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>ILV5_SCHPO (P78827) Probable ketol-acid reductoisomerase, mitochondrial|
           precursor (EC 1.1.1.86) (Acetohydroxy-acid
           reductoisomerase) (Alpha-keto-beta-hydroxylacil
           reductoisomerase)
          Length = 404

 Score = 62.4 bits (150), Expect = 1e-09
 Identities = 30/62 (48%), Positives = 41/62 (66%)
 Frame = -1

Query: 546 LMMAQIEVLRKKGHSYSEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAP 367
           L +AQ +VLR++GHS +E  NE+V E+  SL P +   G+ +M   CSTTAR G+  W P
Sbjct: 277 LFLAQYQVLRERGHSPAEAFNETVEEATQSLYPLIGKYGLDYMFAACSTTARRGAIDWTP 336

Query: 366 RF 361
           RF
Sbjct: 337 RF 338



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>ILV5_YEAST (P06168) Ketol-acid reductoisomerase, mitochondrial precursor (EC|
           1.1.1.86) (Acetohydroxy-acid reductoisomerase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 395

 Score = 61.6 bits (148), Expect = 2e-09
 Identities = 30/62 (48%), Positives = 40/62 (64%)
 Frame = -1

Query: 546 LMMAQIEVLRKKGHSYSEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAP 367
           + +AQ +VLR+ GHS SE  NE+V E+  SL P +   G+ +M D CSTTAR G+  W P
Sbjct: 270 MFLAQYDVLRENGHSPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYP 329

Query: 366 RF 361
            F
Sbjct: 330 IF 331



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>NFRB_ECOLI (P0AFA5) Bacteriophage N4 adsorption protein B|
          Length = 745

 Score = 31.6 bits (70), Expect = 2.3
 Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 3/66 (4%)
 Frame = +1

Query: 469 LDHAL---VDDLRVGVTLLPQHLDLGHHKCNVNSSREGVEWAKVTIWPGRAHLFADLPHA 639
           LD AL   V+ LR+G ++L Q L          + + GV W  +  W   + L A++P +
Sbjct: 505 LDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPAS 564

Query: 640 SLVDFA 657
             + +A
Sbjct: 565 VALHYA 570



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>NFRB_ECO57 (P0AFA6) Bacteriophage N4 adsorption protein B|
          Length = 745

 Score = 31.6 bits (70), Expect = 2.3
 Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 3/66 (4%)
 Frame = +1

Query: 469 LDHAL---VDDLRVGVTLLPQHLDLGHHKCNVNSSREGVEWAKVTIWPGRAHLFADLPHA 639
           LD AL   V+ LR+G ++L Q L          + + GV W  +  W   + L A++P +
Sbjct: 505 LDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPAS 564

Query: 640 SLVDFA 657
             + +A
Sbjct: 565 VALHYA 570



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>SYS_COREF (Q8FLY5) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--tRNA ligase)|
           (SerRS)
          Length = 419

 Score = 30.8 bits (68), Expect = 3.8
 Identities = 20/55 (36%), Positives = 31/55 (56%), Gaps = 4/55 (7%)
 Frame = +1

Query: 211 RHRDVDGGPQLGADLDGAVDGVRHEVA---DEVLVDGRILVHRHKR-LLGQDVVE 363
           R+RD +G PQ+ A L+G +   R  VA   +    DG ++V    R  +G+DV+E
Sbjct: 362 RYRDENGKPQIAATLNGTLATTRWLVAILENNQQADGSVIVPEALRPFVGKDVLE 416



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>ICAA_STAAW (Q8NUI7) Biofilm PIA synthesis N-glycosyltransferase icaA (EC|
           2.4.-.-) (Intercellular adhesion protein A)
          Length = 412

 Score = 30.4 bits (67), Expect = 5.0
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 363 FDYILTQQAFVTVDKDAPINQDLISNFMSDPVHGAI 256
           +DY++   A   VD+DAP    +I NF  DP  GA+
Sbjct: 127 YDYVMCLDADTIVDQDAPYY--MIENFKHDPKLGAV 160



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>ICAA_STAAU (Q9RQP9) Biofilm PIA synthesis N-glycosyltransferase icaA (EC|
           2.4.-.-) (Intercellular adhesion protein A)
          Length = 412

 Score = 30.4 bits (67), Expect = 5.0
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 363 FDYILTQQAFVTVDKDAPINQDLISNFMSDPVHGAI 256
           +DY++   A   VD+DAP    +I NF  DP  GA+
Sbjct: 127 YDYVMCLDADTIVDQDAPYY--MIENFKHDPKLGAV 160



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>ICAA_STAAS (Q6G608) Biofilm PIA synthesis N-glycosyltransferase icaA (EC|
           2.4.-.-) (Intercellular adhesion protein A)
          Length = 412

 Score = 30.4 bits (67), Expect = 5.0
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 363 FDYILTQQAFVTVDKDAPINQDLISNFMSDPVHGAI 256
           +DY++   A   VD+DAP    +I NF  DP  GA+
Sbjct: 127 YDYVMCLDADTIVDQDAPYY--MIENFKHDPKLGAV 160



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>ICAA_STAAR (Q6GDD8) Biofilm PIA synthesis N-glycosyltransferase icaA (EC|
           2.4.-.-) (Intercellular adhesion protein A)
          Length = 412

 Score = 30.4 bits (67), Expect = 5.0
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 363 FDYILTQQAFVTVDKDAPINQDLISNFMSDPVHGAI 256
           +DY++   A   VD+DAP    +I NF  DP  GA+
Sbjct: 127 YDYVMCLDADTIVDQDAPYY--MIENFKHDPKLGAV 160



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>ICAA_STAAN (Q7A351) Biofilm PIA synthesis N-glycosyltransferase icaA (EC|
           2.4.-.-) (Intercellular adhesion protein A)
          Length = 412

 Score = 30.4 bits (67), Expect = 5.0
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 363 FDYILTQQAFVTVDKDAPINQDLISNFMSDPVHGAI 256
           +DY++   A   VD+DAP    +I NF  DP  GA+
Sbjct: 127 YDYVMCLDADTIVDQDAPYY--MIENFKHDPKLGAV 160



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>ICAA_STAAM (Q99QX3) Biofilm PIA synthesis N-glycosyltransferase icaA (EC|
           2.4.-.-) (Intercellular adhesion protein A)
          Length = 412

 Score = 30.4 bits (67), Expect = 5.0
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 363 FDYILTQQAFVTVDKDAPINQDLISNFMSDPVHGAI 256
           +DY++   A   VD+DAP    +I NF  DP  GA+
Sbjct: 127 YDYVMCLDADTIVDQDAPYY--MIENFKHDPKLGAV 160



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>ICAA_STAAC (Q5HCN1) Biofilm PIA synthesis N-glycosyltransferase icaA (EC|
           2.4.-.-) (Intercellular adhesion protein A)
          Length = 412

 Score = 30.4 bits (67), Expect = 5.0
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -1

Query: 363 FDYILTQQAFVTVDKDAPINQDLISNFMSDPVHGAI 256
           +DY++   A   VD+DAP    +I NF  DP  GA+
Sbjct: 127 YDYVMCLDADTIVDQDAPYY--MIENFKHDPKLGAV 160



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>ILVC_DEHE1 (Q3Z891) Ketol-acid reductoisomerase (EC 1.1.1.86)|
           (Acetohydroxy-acid isomeroreductase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 332

 Score = 30.0 bits (66), Expect = 6.6
 Identities = 30/126 (23%), Positives = 55/126 (43%), Gaps = 17/126 (13%)
 Frame = -1

Query: 561 GVYVALMMAQIEVLRKKGHSYSEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGS 382
           G   +L+ A  E L + G+   E+     +  +  +   M+  G+A+M D+ S TA+ G 
Sbjct: 201 GGTTSLVKAGFETLVEAGYQ-PEVAYFECLHELKLIVDLMYQGGIAYMRDSISDTAKYGD 259

Query: 381 RKWAPRFDYILTQQAFVTVDKDAPINQD-------LISNFMSDPVHGAI----------E 253
               PR   ++ ++ + T+ +     QD       ++ N    PV+ ++          E
Sbjct: 260 FTRGPR---VINEETYETMGEILGEIQDGSFAKEWILENQAGRPVYNSLRRMESEHLIEE 316

Query: 252 VCAELR 235
           V AELR
Sbjct: 317 VGAELR 322


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,424,250
Number of Sequences: 219361
Number of extensions: 1953015
Number of successful extensions: 6259
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 5985
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6258
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6484657212
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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