Clone Name | rbags9e11 |
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Clone Library Name | barley_pub |
>CPSF2_ARATH (Q9LKF9) Cleavage and polyadenylation specificity factor, 100 kDa| subunit (CPSF 100 kDa subunit) Length = 739 Score = 229 bits (584), Expect = 6e-60 Identities = 114/163 (69%), Positives = 136/163 (83%), Gaps = 3/163 (1%) Frame = -3 Query: 666 HLKMHCAKNSDLHVYAPQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLGEHEIAWVDAG 487 HLK HC N HVYAPQ+EET+DVTSDLCAYKVQLSEKLMSNVI KKLG+ E+AWVD+ Sbjct: 577 HLKQHCLNNICPHVYAPQIEETVDVTSDLCAYKVQLSEKLMSNVIFKKLGDSEVAWVDSE 636 Query: 486 VGKADEKL-SLVPPSSIPAAHNSVLVGDLKLADFKQFLANKGLQVEFA-GGALRCGEYIT 313 VGK + + SL+P + H VLVGDLK+ADFKQFL++KG+QVEFA GGALRCGEY+T Sbjct: 637 VGKTERDMRSLLPMPGAASPHKPVLVGDLKIADFKQFLSSKGVQVEFAGGGALRCGEYVT 696 Query: 312 VRKIGDSNQK-GSTGSQQIVIEGPLCEDYYKIRELLYSQFFLL 187 +RK+G + QK G++G QQI+IEGPLCEDYYKIR+ LYSQF+LL Sbjct: 697 LRKVGPTGQKGGASGPQQILIEGPLCEDYYKIRDYLYSQFYLL 739
>CPSF2_DROME (Q9V3D6) Probable cleavage and polyadenylation specificity factor,| 100 kDa subunit (CPSF 100 kDa subunit) Length = 756 Score = 100 bits (250), Expect = 3e-21 Identities = 65/179 (36%), Positives = 96/179 (53%), Gaps = 23/179 (12%) Frame = -3 Query: 654 HCAKNSDLHVYAPQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLGEHEIAWVDAGVG-- 481 HC +N V+ PQ E IDVTS++ Y+V+L+E L+S + +K + E+AWVD +G Sbjct: 588 HCEQNVGARVFTPQKGEIIDVTSEIHIYQVRLTEGLVSQLQFQKGKDAEVAWVDGRLGMR 647 Query: 480 -KADE-------------------KLSLVPPSSIPAAHNSVLVGDLKLADFKQFLANKGL 361 KA E L + IP HNSVL+ +LKL+DFKQ L + Sbjct: 648 VKAIEAPMDVTVEQDASVQEGKTLTLETLADDEIP-IHNSVLINELKLSDFKQTLMRNNI 706 Query: 360 QVEFAGGALRCGE-YITVRKIGDSNQKGSTGSQQIVIEGPLCEDYYKIRELLYSQFFLL 187 EF+GG L C + +R++ + ++ +EG L E+YYKIRELLY Q+ ++ Sbjct: 707 NSEFSGGVLWCSNGTLALRRV---------DAGKVAMEGCLSEEYYKIRELLYEQYAIV 756
>CPSF2_XENLA (Q9W799) Cleavage and polyadenylation specificity factor, 100 kDa| subunit (CPSF 100 kDa subunit) Length = 783 Score = 90.5 bits (223), Expect = 4e-18 Identities = 61/208 (29%), Positives = 94/208 (45%), Gaps = 58/208 (27%) Frame = -3 Query: 636 DLHVYAPQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLGEHEIAWVDA----GVGKAD- 472 D+ VY P+L ET+D TS+ Y+V+L + L+S++ K + E+AW+D V K D Sbjct: 585 DIKVYTPKLHETVDATSETHIYQVRLKDSLVSSLKFCKAKDTELAWIDGVLDMRVSKVDT 644 Query: 471 ------------------------------------------------EKLSLVP----- 451 E+ ++P Sbjct: 645 GVILEERELKDEGEDMEMQVDTQVMDASTIAQQKVIKSLFGDDDKEFSEESEIIPTLEPL 704 Query: 450 PSSIPAAHNSVLVGDLKLADFKQFLANKGLQVEFAGGALRCGEYITVRKIGDSNQKGSTG 271 PS+ H SV + + +L+DFKQ L +G+ EF GG L C + VR+ T Sbjct: 705 PSNEVPGHQSVFMNEPRLSDFKQVLLREGIHAEFVGGVLVCNNMVAVRR---------TE 755 Query: 270 SQQIVIEGPLCEDYYKIRELLYSQFFLL 187 + +I +EG LCED++KIRELLY Q+ ++ Sbjct: 756 TGRIGLEGCLCEDFFKIRELLYEQYAIV 783
>CPSF2_MOUSE (O35218) Cleavage and polyadenylation specificity factor, 100 kDa| subunit (CPSF 100 kDa subunit) Length = 782 Score = 90.5 bits (223), Expect = 4e-18 Identities = 65/208 (31%), Positives = 97/208 (46%), Gaps = 58/208 (27%) Frame = -3 Query: 636 DLHVYAPQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLGEHEIAW-----------VDA 490 D+ VY P+L ET+D TS+ Y+V+L + L+S++ K + E+AW VD Sbjct: 585 DIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAWIDGVLDMRVSKVDT 644 Query: 489 GV-----------------------------GKA-------DEK-----------LSLVP 451 GV KA DEK L +P Sbjct: 645 GVILEEGELKDDGEDSEMQVDAPSDSSAMAQQKAMKSLFGEDEKELGEETEIIPTLEPLP 704 Query: 450 PSSIPAAHNSVLVGDLKLADFKQFLANKGLQVEFAGGALRCGEYITVRKIGDSNQKGSTG 271 P +P H SV + + +L+DFKQ L +G+Q EF GG L C + VR+ T Sbjct: 705 PHEVPG-HQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRR---------TE 754 Query: 270 SQQIVIEGPLCEDYYKIRELLYSQFFLL 187 + +I +EG LC+D+Y+IR+LLY Q+ ++ Sbjct: 755 TGRIGLEGCLCQDFYRIRDLLYEQYAIV 782
>CPSF2_HUMAN (Q9P2I0) Cleavage and polyadenylation specificity factor, 100 kDa| subunit (CPSF 100 kDa subunit) Length = 782 Score = 90.5 bits (223), Expect = 4e-18 Identities = 65/208 (31%), Positives = 97/208 (46%), Gaps = 58/208 (27%) Frame = -3 Query: 636 DLHVYAPQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLGEHEIAW-----------VDA 490 D+ VY P+L ET+D TS+ Y+V+L + L+S++ K + E+AW VD Sbjct: 585 DIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAWIDGVLDMRVSKVDT 644 Query: 489 GV-----------------------------GKA-------DEK-----------LSLVP 451 GV KA DEK L +P Sbjct: 645 GVILEEGELKDDGEDSEMQVEAPSDSSVIAQQKAMKSLFGDDEKETGEESEIIPTLEPLP 704 Query: 450 PSSIPAAHNSVLVGDLKLADFKQFLANKGLQVEFAGGALRCGEYITVRKIGDSNQKGSTG 271 P +P H SV + + +L+DFKQ L +G+Q EF GG L C + VR+ T Sbjct: 705 PHEVPG-HQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRR---------TE 754 Query: 270 SQQIVIEGPLCEDYYKIRELLYSQFFLL 187 + +I +EG LC+D+Y+IR+LLY Q+ ++ Sbjct: 755 TGRIGLEGCLCQDFYRIRDLLYEQYAIV 782
>CPSF2_BOVIN (Q10568) Cleavage and polyadenylation specificity factor, 100 kDa| subunit (CPSF 100 kDa subunit) Length = 782 Score = 90.5 bits (223), Expect = 4e-18 Identities = 65/208 (31%), Positives = 97/208 (46%), Gaps = 58/208 (27%) Frame = -3 Query: 636 DLHVYAPQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLGEHEIAW-----------VDA 490 D+ VY P+L ET+D TS+ Y+V+L + L+S++ K + E+AW VD Sbjct: 585 DIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAWIDGVLDMRVSKVDT 644 Query: 489 GV-----------------------------GKA-------DEK-----------LSLVP 451 GV KA DEK L +P Sbjct: 645 GVILEEGELKDDGEDSEMQVDAPSDSSVIAQQKAMKSLFGDDEKETGEESEIIPTLEPLP 704 Query: 450 PSSIPAAHNSVLVGDLKLADFKQFLANKGLQVEFAGGALRCGEYITVRKIGDSNQKGSTG 271 P +P H SV + + +L+DFKQ L +G+Q EF GG L C + VR+ T Sbjct: 705 PHEVPG-HQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRR---------TE 754 Query: 270 SQQIVIEGPLCEDYYKIRELLYSQFFLL 187 + +I +EG LC+D+Y+IR+LLY Q+ ++ Sbjct: 755 TGRIGLEGCLCQDFYRIRDLLYEQYAIV 782
>CPSF2_CAEEL (O17403) Probable cleavage and polyadenylation specificity factor,| 100 kDa subunit (CPSF 100 kDa subunit) Length = 843 Score = 57.0 bits (136), Expect = 5e-08 Identities = 38/100 (38%), Positives = 50/100 (50%), Gaps = 1/100 (1%) Frame = -3 Query: 483 GKADEKLSLVP-PSSIPAAHNSVLVGDLKLADFKQFLANKGLQVEFAGGALRCGEYITVR 307 GK L L P P + H +V V D KL+DFK L +KG + EF G L + Sbjct: 752 GKIRGNLILDPLPKRLIPIHQAVFVNDPKLSDFKNLLTDKGYKAEFLSGTL------LIN 805 Query: 306 KIGDSNQKGSTGSQQIVIEGPLCEDYYKIRELLYSQFFLL 187 S ++ TG Q +EG +DYYK+R L Y QF +L Sbjct: 806 GGNCSIRRNDTGVFQ--MEGAFTKDYYKLRRLFYDQFAVL 843 Score = 32.3 bits (72), Expect = 1.4 Identities = 15/47 (31%), Positives = 30/47 (63%), Gaps = 1/47 (2%) Frame = -3 Query: 621 APQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLGE-HEIAWVDAGV 484 AP+ +D + + Y+V LS+ L++++ K++ E + +AW+DA V Sbjct: 624 APEAGALVDASVESFIYQVALSDALLADIQFKEVSEGNSLAWIDARV 670
>MTLD_KLEPN (Q9XBM6) Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17)| Length = 382 Score = 31.6 bits (70), Expect = 2.4 Identities = 18/71 (25%), Positives = 37/71 (52%) Frame = -3 Query: 579 CAYKVQLSEKLMSNVISKKLGEHEIAWVDAGVGKADEKLSLVPPSSIPAAHNSVLVGDLK 400 C V+ + +L +V + L E + AWV+A +G D + + P S A H+ + ++ Sbjct: 120 CENMVRGTTQLKGHVFNA-LAEEDKAWVEAHIGFVDSAVDRIVPPSASATHDPL---EVT 175 Query: 399 LADFKQFLANK 367 + F +++ +K Sbjct: 176 VETFSEWIVDK 186
>YM81_YEAST (Q04781) RING finger protein YMR247C| Length = 1562 Score = 30.8 bits (68), Expect = 4.1 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = +2 Query: 626 TCKSEFFAQCIFKWFSSFG 682 TCK++F C++KWF S G Sbjct: 1529 TCKNKFHGACLYKWFRSSG 1547
>SCM_DROME (Q9VHA0) Polycomb protein Scm (Sex comb on midleg protein)| Length = 877 Score = 30.4 bits (67), Expect = 5.4 Identities = 21/90 (23%), Positives = 38/90 (42%), Gaps = 8/90 (8%) Frame = +3 Query: 138 HRSFVQHTTMAQRLPSTTRRIVNKGARGSCSSPRTA--------GPQSQSAESPCYPFG* 293 H+S + ++ QR P +V + +SP TA GP +++ PC G Sbjct: 384 HKSRMDSSSSKQRCPRPRYTVVAESEAMVPASPATAHFHPNCKGGPFINNSKLPCMVTGP 443 Query: 294 NHQSCAR*CTHRTVEHPQQTQPVNLYLLGI 383 +Q+ A+ C + TQ ++ L + Sbjct: 444 TYQTLAKLCLQEVLAASTDTQQLSKLLFAL 473
>ZN294_MOUSE (Q6A009) Zinc finger protein 294 (Zfp-294)| Length = 1767 Score = 30.0 bits (66), Expect = 7.1 Identities = 9/17 (52%), Positives = 14/17 (82%) Frame = +2 Query: 626 TCKSEFFAQCIFKWFSS 676 TCK +F + C++KWF+S Sbjct: 1737 TCKKKFHSACLYKWFTS 1753
>ZN294_HUMAN (O94822) Zinc finger protein 294| Length = 1766 Score = 30.0 bits (66), Expect = 7.1 Identities = 9/17 (52%), Positives = 14/17 (82%) Frame = +2 Query: 626 TCKSEFFAQCIFKWFSS 676 TCK +F + C++KWF+S Sbjct: 1736 TCKKKFHSACLYKWFTS 1752
>P5CS_CAEEL (P54889) Probable delta 1-pyrroline-5-carboxylate synthetase (P5CS)| [Includes: Glutamate 5-kinase (EC 2.7.2.11) (Gamma-glutamyl kinase) (GK); Gamma-glutamyl phosphate reductase (GPR) (EC 1.2.1.41) (Glutamate-5-semialdehyde dehydrogenase) (Glut Length = 800 Score = 30.0 bits (66), Expect = 7.1 Identities = 17/55 (30%), Positives = 27/55 (49%) Frame = -3 Query: 681 PKLLNHLKMHCAKNSDLHVYAPQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLG 517 P+LLN LKM K DLH + ++ + KV++SE L ++ +G Sbjct: 412 PQLLNRLKMTPEKIQDLHAGLNTIADSAETLVGRVLKKVKISEGLFLEQVTVPIG 466
>NKRF_HUMAN (O15226) NF-kappa-B-repressing factor (NFKB-repressing factor)| (Transcription factor NRF) (ITBA4 protein) Length = 690 Score = 29.6 bits (65), Expect = 9.2 Identities = 24/88 (27%), Positives = 39/88 (44%), Gaps = 2/88 (2%) Frame = -3 Query: 684 QPKLLNHLKMHCAKN--SDLHVYAPQLEETIDVTSDLCAYKVQLSEKLMSNVISKKLGEH 511 QP ++N+LK ++ S + EE AYK Q+ E + N + +K+G Sbjct: 515 QPTVINNLKKGAVEDVISRNEIQGRSAEE---------AYKQQIKEDNIGNQLLRKMG-- 563 Query: 510 EIAWVDAGVGKADEKLSLVPPSSIPAAH 427 W G+GK+ E + P S+ H Sbjct: 564 ---WTGGGLGKSGE--GIREPISVKEQH 586 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 98,165,222 Number of Sequences: 219361 Number of extensions: 1979222 Number of successful extensions: 5196 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 4987 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5183 length of database: 80,573,946 effective HSP length: 109 effective length of database: 56,663,597 effective search space used: 6969622431 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)