ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl55b02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1PDIA6_ARATH (O22263) Probable protein disulfide-isomerase A6 pre... 34 0.098
2RIB4_MAGGR (Q9UVT8) 6,7-dimethyl-8-ribityllumazine synthase (EC ... 33 0.22
3ERP60_SCHMA (P38658) Probable protein disulfide-isomerase ER-60 ... 32 0.28
4PDI_YEAST (P17967) Protein disulfide-isomerase precursor (EC 5.3... 32 0.48
5PDI2_CAEEL (Q17770) Protein disulfide-isomerase 2 precursor (EC ... 31 0.83
6PDI1_SCHPO (Q10057) Putative protein disulfide-isomerase C1F5.02... 30 1.1
7REXO4_NEUCR (Q7S9B7) RNA exonuclease 4 (EC 3.1.-.-) 30 1.8
8ERP38_NEUCR (Q92249) Protein disulfide-isomerase erp38 precursor... 29 2.4
9BXF_CLOBO (P30996) Botulinum neurotoxin type F precursor (EC 3.4... 29 2.4
10PDIA6_MEDSA (P38661) Probable protein disulfide-isomerase A6 pre... 29 2.4
11TSC1_HUMAN (Q92574) Hamartin (Tuberous sclerosis 1 protein) 28 4.1
12TIGA_ASPNG (Q00216) Protein disulfide-isomerase tigA precursor (... 28 4.1
13DPO3A_MYCGE (Q49405) DNA polymerase III alpha subunit (EC 2.7.7.7) 28 4.1
14PDI1_CAEEL (Q17967) Protein disulfide-isomerase 1 precursor (EC ... 28 5.4
15GCST_PYRHO (O58888) Probable aminomethyltransferase (EC 2.1.2.10... 28 5.4
16MLO8_ARATH (O22757) MLO-like protein 8 (AtMlo8) 28 7.0
17YJ9G_YEAST (P47170) Hypothetical 182.0 kDa protein in NMD5-HOM6 ... 28 7.0
18Y792_SULAC (Q4JAL7) Protein Saci_0792 27 9.1
19YKGB_BACSU (O34499) Hypothetical protein ykgB 27 9.1
20PDI_HORVU (P80284) Protein disulfide-isomerase precursor (EC 5.3... 27 9.1
21RNH_XANCP (Q8PBX8) Ribonuclease H (EC 3.1.26.4) (RNase H) 27 9.1
22POLG_PVYHU (Q02963) Genome polyprotein [Contains: P1 proteinase ... 27 9.1

>PDIA6_ARATH (O22263) Probable protein disulfide-isomerase A6 precursor (EC|
           5.3.4.1) (P5)
          Length = 361

 Score = 33.9 bits (76), Expect = 0.098
 Identities = 15/52 (28%), Positives = 25/52 (48%)
 Frame = -1

Query: 266 DCHHLLKSADKEEFYPTVLLFPAENKSAISYEGGISVGNLIEFLELHASNSR 111
           D H  L        +PT+  FP +NK+   Y+GG  + + + F+   +  SR
Sbjct: 202 DAHKALGEKYGVSGFPTLKFFPKDNKAGHDYDGGRDLDDFVSFINEKSGTSR 253



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>RIB4_MAGGR (Q9UVT8) 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.9) (DMRL|
           synthase) (Lumazine synthase) (Riboflavin synthase beta
           chain)
          Length = 200

 Score = 32.7 bits (73), Expect = 0.22
 Identities = 23/75 (30%), Positives = 37/75 (49%), Gaps = 4/75 (5%)
 Frame = +1

Query: 31  DYSTSRIQILTYEYQTLLNQPLYSNIWRELLACNSKNSIKLPTEIPPSYDIAL----LFS 198
           D S  RI I+   +   + +PL +    +LLAC  K S  +   +P S+++ +    L+S
Sbjct: 12  DGSALRIGIVHARWNETIIEPLLAGTKAKLLACGVKESNIVVQSVPGSWELPIAVQRLYS 71

Query: 199 AGKSNTVG*NSSLSA 243
           A +  T     SLSA
Sbjct: 72  ASQLQTPSSGPSLSA 86



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>ERP60_SCHMA (P38658) Probable protein disulfide-isomerase ER-60 precursor (EC|
           5.3.4.1) (ERP60)
          Length = 484

 Score = 32.3 bits (72), Expect = 0.28
 Identities = 19/56 (33%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
 Frame = -1

Query: 284 MDCTTNDCHHLLKSADKEEFYPTVLLFPAENKSA-ISYEGGISVGNLIEFLELHAS 120
           MD T ND    + S  +   +PT+   P   KS+ +SYEGG    ++I++L   A+
Sbjct: 416 MDATAND----VPSPYQVRGFPTIYFVPKGKKSSPVSYEGGRDTNDIIKYLAREAT 467



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>PDI_YEAST (P17967) Protein disulfide-isomerase precursor (EC 5.3.4.1) (PDI)|
           (Thioredoxin-related glycoprotein 1)
          Length = 522

 Score = 31.6 bits (70), Expect = 0.48
 Identities = 18/55 (32%), Positives = 32/55 (58%), Gaps = 1/55 (1%)
 Frame = -1

Query: 293 IYMMDCTTNDCHHLLKSADKEEFYPTVLLFPAENKS-AISYEGGISVGNLIEFLE 132
           I  +D T ND   ++      E YPT++L+P   KS ++ Y+G  S+ +L +F++
Sbjct: 432 IAKLDHTENDVRGVVI-----EGYPTIVLYPGGKKSESVVYQGSRSLDSLFDFIK 481



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>PDI2_CAEEL (Q17770) Protein disulfide-isomerase 2 precursor (EC 5.3.4.1) (PDI|
           1) (Prolyl 4-hydroxylase beta subunit)
          Length = 493

 Score = 30.8 bits (68), Expect = 0.83
 Identities = 16/54 (29%), Positives = 26/54 (48%)
 Frame = -1

Query: 293 IYMMDCTTNDCHHLLKSADKEEFYPTVLLFPAENKSAISYEGGISVGNLIEFLE 132
           I  MD T N+   +     K + +PT+  FPA +   + Y G  ++    +FLE
Sbjct: 418 IAKMDSTLNEVEDV-----KIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLE 466



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>PDI1_SCHPO (Q10057) Putative protein disulfide-isomerase C1F5.02 precursor (EC|
           5.3.4.1)
          Length = 492

 Score = 30.4 bits (67), Expect = 1.1
 Identities = 20/70 (28%), Positives = 36/70 (51%), Gaps = 1/70 (1%)
 Frame = -1

Query: 326 EENNGDLGLPAIYMMDCTTNDCHHLLKSADKEEFYPTVLLFPAENK-SAISYEGGISVGN 150
           EE + D  +  +  +D T ND    +        +PT++ F A +K + + YEG  ++ +
Sbjct: 400 EEYSDDSNV-VVAKIDATENDISVSISG------FPTIMFFKANDKVNPVRYEGDRTLED 452

Query: 149 LIEFLELHAS 120
           L  F++ HAS
Sbjct: 453 LSAFIDKHAS 462



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>REXO4_NEUCR (Q7S9B7) RNA exonuclease 4 (EC 3.1.-.-)|
          Length = 406

 Score = 29.6 bits (65), Expect = 1.8
 Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 14/45 (31%)
 Frame = +1

Query: 130 NSKNSIKLPTEIPPSY--------------DIALLFSAGKSNTVG 222
           ++KN + LPT++P S               D+AL+  A KSNT+G
Sbjct: 170 SNKNGLPLPTDLPSSLTLSNGLTLDTSTTTDLALILQATKSNTLG 214



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>ERP38_NEUCR (Q92249) Protein disulfide-isomerase erp38 precursor (EC 5.3.4.1)|
           (ERp38)
          Length = 369

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 13/38 (34%), Positives = 20/38 (52%)
 Frame = -1

Query: 224 YPTVLLFPAENKSAISYEGGISVGNLIEFLELHASNSR 111
           +PT+  FP  + +   Y GG S  +L++FL   A   R
Sbjct: 217 FPTIKFFPKGSTTPEDYNGGRSEADLVKFLNEKAGTHR 254



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>BXF_CLOBO (P30996) Botulinum neurotoxin type F precursor (EC 3.4.24.69)|
           (BoNT/F) (Bontoxilysin F) [Contains: Botulinum
           neurotoxin F light chain; Botulinum neurotoxin F heavy
           chain]
          Length = 1274

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 22/59 (37%), Positives = 29/59 (49%), Gaps = 2/59 (3%)
 Frame = +2

Query: 89  NLYIPTYGENCSHATPKIQLNYPQKFLLHTILHFCSP--PEKATP*DKTPPCLLILVND 259
           NL +   G++     PKI  + P K L+  I+ FC    P K T   K PP L I VN+
Sbjct: 396 NLAVNNRGQSIK-LNPKIIDSIPDKGLVEKIVKFCKSVIPRKGT---KAPPRLCIRVNN 450



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>PDIA6_MEDSA (P38661) Probable protein disulfide-isomerase A6 precursor (EC|
           5.3.4.1) (P5)
          Length = 364

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 12/38 (31%), Positives = 20/38 (52%)
 Frame = -1

Query: 224 YPTVLLFPAENKSAISYEGGISVGNLIEFLELHASNSR 111
           +PT+  FP  NK+   Y GG  + + + F+   +  SR
Sbjct: 222 FPTLKFFPKGNKAGEDYGGGRDLDDFVAFINEKSGTSR 259



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>TSC1_HUMAN (Q92574) Hamartin (Tuberous sclerosis 1 protein)|
          Length = 1164

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 21/84 (25%), Positives = 34/84 (40%), Gaps = 10/84 (11%)
 Frame = +1

Query: 1   ENSCEQ*TNIDYSTSRIQILTYEYQT----------LLNQPLYSNIWRELLACNSKNSIK 150
           +NS    T+  YSTSR+ +L    Q           L+ +P  + +W   + C       
Sbjct: 301 QNSYGCATSTPYSTSRLMLLNMPGQLPQTLSSPSTRLITEPPQATLWSPSMVCGMTTPPT 360

Query: 151 LPTEIPPSYDIALLFSAGKSNTVG 222
            P  +PP  D++  +S     T G
Sbjct: 361 SPGNVPP--DLSHPYSKVFGTTAG 382



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>TIGA_ASPNG (Q00216) Protein disulfide-isomerase tigA precursor (EC 5.3.4.1)|
          Length = 359

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -1

Query: 224 YPTVLLFPAENKSAISYEGGISVGNLIEFL 135
           YPT+  FP  +  ++ YEG  S    I+FL
Sbjct: 216 YPTIKFFPKGSTESVPYEGARSEQAFIDFL 245



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>DPO3A_MYCGE (Q49405) DNA polymerase III alpha subunit (EC 2.7.7.7)|
          Length = 874

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 20/59 (33%), Positives = 27/59 (45%), Gaps = 9/59 (15%)
 Frame = +2

Query: 59  LRTSTRHF*TNLYIPTYGENCSHA---------TPKIQLNYPQKFLLHTILHFCSPPEK 208
           L+T+T+ F        YG N SHA         T  ++ NYP  F L  + HF S  +K
Sbjct: 676 LKTTTKIFSYIERFANYGFNLSHALGYALLSYWTAWLKTNYPVYFYLWLLNHFQSSKDK 734



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>PDI1_CAEEL (Q17967) Protein disulfide-isomerase 1 precursor (EC 5.3.4.1) (PDI|
           1) (Prolyl 4-hydroxylase beta 1 subunit)
          Length = 485

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 15/60 (25%), Positives = 31/60 (51%)
 Frame = -1

Query: 293 IYMMDCTTNDCHHLLKSADKEEFYPTVLLFPAENKSAISYEGGISVGNLIEFLELHASNS 114
           I  +D T N+   +     K   +PT+ L+PA + + + Y+G  ++    EF+  +A ++
Sbjct: 418 IAKLDATLNELADV-----KVNSFPTLKLWPAGSSTPVDYDGDRNLEKFEEFVNKYAGSA 472



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>GCST_PYRHO (O58888) Probable aminomethyltransferase (EC 2.1.2.10) (Glycine|
           cleavage system T protein)
          Length = 398

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 8/30 (26%), Positives = 17/30 (56%)
 Frame = -1

Query: 146 IEFLELHASNSRHMLEYKGWFKSVWYSYVR 57
           +   + H  ++R + E+ GW   +WYS ++
Sbjct: 5   VHIFDWHKEHARKIEEFAGWEMPIWYSSIK 34



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>MLO8_ARATH (O22757) MLO-like protein 8 (AtMlo8)|
          Length = 593

 Score = 27.7 bits (60), Expect = 7.0
 Identities = 16/60 (26%), Positives = 29/60 (48%)
 Frame = +1

Query: 37  STSRIQILTYEYQTLLNQPLYSNIWRELLACNSKNSIKLPTEIPPSYDIALLFSAGKSNT 216
           STS ++ L   Y+T  +   Y  +  E    ++ N    P + PPS+++ +     K+NT
Sbjct: 506 STSSVRSLQ-RYKTTPHSMRYEGLDPETSDLDTDNEALTPPKSPPSFELVVKVEPNKTNT 564



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>YJ9G_YEAST (P47170) Hypothetical 182.0 kDa protein in NMD5-HOM6 intergenic|
           region
          Length = 1584

 Score = 27.7 bits (60), Expect = 7.0
 Identities = 13/27 (48%), Positives = 18/27 (66%)
 Frame = +1

Query: 40  TSRIQILTYEYQTLLNQPLYSNIWREL 120
           TS + IL+ + QTLL+ P  S IW +L
Sbjct: 169 TSNVSILSGQLQTLLDLPSRSRIWIKL 195



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>Y792_SULAC (Q4JAL7) Protein Saci_0792|
          Length = 227

 Score = 27.3 bits (59), Expect = 9.1
 Identities = 9/23 (39%), Positives = 15/23 (65%)
 Frame = -3

Query: 267 RLPSFTKISRQGGVLSYGVAFSG 199
           +LPSF  +   G ++ YG+ +SG
Sbjct: 129 KLPSFINVGEDGLIVEYGIMYSG 151



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>YKGB_BACSU (O34499) Hypothetical protein ykgB|
          Length = 349

 Score = 27.3 bits (59), Expect = 9.1
 Identities = 17/61 (27%), Positives = 29/61 (47%)
 Frame = -3

Query: 375 LYEFKFSVCEGSRFADRRE*RRLGPSSYLHDGLHNKRLPSFTKISRQGGVLSYGVAFSGG 196
           +Y F+    E    ++ +   +LG  +Y+    +N  L S  K   QGGV +Y +  + G
Sbjct: 19  IYSFELDT-EKKALSEPKLAAKLGNPTYVATNKNNTILYSIEKADGQGGVAAYQIDKNSG 77

Query: 195 E 193
           E
Sbjct: 78  E 78



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>PDI_HORVU (P80284) Protein disulfide-isomerase precursor (EC 5.3.4.1) (PDI)|
           (Endosperm protein E-1)
          Length = 513

 Score = 27.3 bits (59), Expect = 9.1
 Identities = 21/70 (30%), Positives = 33/70 (47%)
 Frame = -1

Query: 344 AQGLQTEENNGDLGLPAIYMMDCTTNDCHHLLKSADKEEFYPTVLLFPAENKSAISYEGG 165
           A  LQ+EE+        I  MD T ND    +      + YPT L F   +   +SYEGG
Sbjct: 425 AATLQSEED------VVIAKMDATEND----VPGEFDVQGYPT-LYFVTPSGKKVSYEGG 473

Query: 164 ISVGNLIEFL 135
            +   +++++
Sbjct: 474 RTADEIVDYI 483



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>RNH_XANCP (Q8PBX8) Ribonuclease H (EC 3.1.26.4) (RNase H)|
          Length = 150

 Score = 27.3 bits (59), Expect = 9.1
 Identities = 23/88 (26%), Positives = 34/88 (38%)
 Frame = -1

Query: 344 AQGLQTEENNGDLGLPAIYMMDCTTNDCHHLLKSADKEEFYPTVLLFPAENKSAISYEGG 165
           A G     NN    + AI  ++  T  C  LL +  +          P   +      GG
Sbjct: 35  AGGEANSTNNRMELMAAIMALETLTEPCQILLHTDSQYVRQGITEWMPGWVRRGWKTSGG 94

Query: 164 ISVGNLIEFLELHASNSRHMLEYKGWFK 81
             V N   +  LHA+  RH +E++ W K
Sbjct: 95  DPVKNRELWERLHAATQRHSIEWR-WVK 121



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>POLG_PVYHU (Q02963) Genome polyprotein [Contains: P1 proteinase (N-terminal|
           protein); Helper component proteinase (EC 3.4.22.45)
           (HC-pro); Protein P3; 6 kDa protein 1 (6K1); Cytoplasmic
           inclusion protein (EC 3.6.1.-) (CI); 6 kDa protein 2
           (6K2); Viral ge
          Length = 3061

 Score = 27.3 bits (59), Expect = 9.1
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = +1

Query: 34  YSTSRIQILTYEYQTLLNQPLYSNIWRELLA 126
           Y+TS +QI+   Y  LLN       WRE L+
Sbjct: 902 YNTSVVQIMEKNYLNLLNDAWKDLTWREKLS 932


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,568,192
Number of Sequences: 219361
Number of extensions: 1148876
Number of successful extensions: 2951
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 2895
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2950
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 1391514312
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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