ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl53d03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MET2_SCHPO (O60062) Homoserine O-acetyltransferase (EC 2.3.1.31)... 30 1.5
2NU2M_ASTPE (Q33819) NADH-ubiquinone oxidoreductase chain 2 (EC 1... 30 1.9
3SCW11_YEAST (P53189) Probable family 17 glucosidase SCW11 precur... 30 1.9
4MTAL1_KLULA (Q08398) Mating-type protein ALPHA1 (Transcription a... 30 1.9
5TILS_BUCBP (Q89AX3) tRNA(Ile)-lysidine synthase (EC 6.3.4.-) (tR... 30 1.9
6CCNB3_MOUSE (Q810T2) G2/mitotic-specific cyclin-B3 29 2.5
7PSTC2_MYCTU (P0A630) Phosphate transport system permease protein... 29 2.5
8PSTC2_MYCBO (P0A631) Phosphate transport system permease protein... 29 2.5
9SEC1_YEAST (P30619) Protein transport protein SEC1 28 4.2
10PSTC_MYCLE (Q50098) Phosphate transport system permease protein ... 28 5.5
11AFAD_HUMAN (P55196) Afadin (AF-6 protein) 28 5.5
12NAD2_CAEEL (P32739) Sodium-dependent high-affinity dicarboxylate... 28 7.2
13AF17_HUMAN (P55198) Protein AF-17 28 7.2
14IMMB_ECOLI (P22426) Colicin-B immunity protein (Microcin-B immun... 27 9.4
15YKG6_CAEEL (P46556) Hypothetical protein B0285.6 in chromosome III 27 9.4
16TBP_BOMMO (O45211) TATA-box-binding protein (TATA-box factor) (T... 27 9.4

>MET2_SCHPO (O60062) Homoserine O-acetyltransferase (EC 2.3.1.31) (Homoserine|
           O-trans-acetylase)
          Length = 489

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 17/61 (27%), Positives = 27/61 (44%)
 Frame = +1

Query: 145 HWRRHKNIPFDRRRKKKPCSFPGSSKS*AAVAERPKSMASSFPPNNNQHSCNLAKIYSSK 324
           HW  H     +R R+++PC   GSS +  +    P S  SS P      + + + +  S 
Sbjct: 274 HWVVHNE--GNRNRRERPCRSNGSSPTSESALNSPASSVSSLPSLGASQTTDSSSLNQSS 331

Query: 325 L 327
           L
Sbjct: 332 L 332



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>NU2M_ASTPE (Q33819) NADH-ubiquinone oxidoreductase chain 2 (EC 1.6.5.3) (NADH|
           dehydrogenase subunit 2)
          Length = 354

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 8/64 (12%)
 Frame = -1

Query: 221 LLEPGNEQGFFFLLLSNGMFLCLLQCYYFFSVLTQFVTMYCG--------FGVFCRLQYI 66
           L+EP N+   F + L+ G+ L L  C+Y+F  + Q V    G           F  L Y+
Sbjct: 86  LMEPLNQATSFLMTLALGLKLGLFPCHYWFPDVIQGVGFIQGLVLSTWQKIAPFAVLVYV 145

Query: 65  VSAL 54
           V +L
Sbjct: 146 VESL 149



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>SCW11_YEAST (P53189) Probable family 17 glucosidase SCW11 precursor (EC|
           3.2.1.-) (Soluble cell wall protein 11)
          Length = 542

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 17/41 (41%), Positives = 23/41 (56%)
 Frame = +1

Query: 211 GSSKS*AAVAERPKSMASSFPPNNNQHSCNLAKIYSSKLAV 333
           GSS S AA+   PK++A  + P N+  SC  A   SS L +
Sbjct: 269 GSSSSSAAITSSPKAIA--YSPYNDDGSCKSADAVSSDLTL 307



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>MTAL1_KLULA (Q08398) Mating-type protein ALPHA1 (Transcription activator|
           Alpha1p) (MATalpha1 protein)
          Length = 261

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +2

Query: 272 LQTIINTHVTSQKYTAQNWLLLTSQFLC 355
           L TII+ H T  K T +NW L+  ++ C
Sbjct: 129 LSTIISKHWTVDKQTRKNWELIAQEYNC 156



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>TILS_BUCBP (Q89AX3) tRNA(Ile)-lysidine synthase (EC 6.3.4.-)|
           (tRNA(Ile)-lysidine synthetase)
           (tRNA(Ile)-2-lysyl-cytidine synthase)
          Length = 438

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 15/42 (35%), Positives = 24/42 (57%)
 Frame = +3

Query: 3   RLTVISQNNLRFSFRLLQC*HDIL*PTEYSKTTVHCYKLCKN 128
           +L  + +NNL F+FR +   H +   +E  K + HC K+C N
Sbjct: 30  QLLKLKKNNLNFTFRAIHINHQLHPDSE--KWSDHCKKICIN 69



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>CCNB3_MOUSE (Q810T2) G2/mitotic-specific cyclin-B3|
          Length = 1396

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = -2

Query: 127  FLHNL*QCTVVLEYSVGYNISCQHCRSRKLN 35
            ++ NL  C   LEY  GY ++  H   RKLN
Sbjct: 1319 YMRNLSNCVPTLEYFTGYKMAELHILVRKLN 1349



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>PSTC2_MYCTU (P0A630) Phosphate transport system permease protein pstC-2|
          Length = 324

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 16/57 (28%), Positives = 25/57 (43%)
 Frame = -3

Query: 297 T*VLIIVWRKGRSHGFWSLGDGGLTLAGAWE*TRLFFSSSVKWDVFVSPPMLLFLFS 127
           T  ++++ R     G WSL DGG T A         FS  +    ++S    LF+ +
Sbjct: 251 TVAVLVILRSAARPGTWSLFDGGYTFASKIASAASEFSEPLPTGAYISAGFALFVLT 307



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>PSTC2_MYCBO (P0A631) Phosphate transport system permease protein pstC-2|
          Length = 324

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 16/57 (28%), Positives = 25/57 (43%)
 Frame = -3

Query: 297 T*VLIIVWRKGRSHGFWSLGDGGLTLAGAWE*TRLFFSSSVKWDVFVSPPMLLFLFS 127
           T  ++++ R     G WSL DGG T A         FS  +    ++S    LF+ +
Sbjct: 251 TVAVLVILRSAARPGTWSLFDGGYTFASKIASAASEFSEPLPTGAYISAGFALFVLT 307



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>SEC1_YEAST (P30619) Protein transport protein SEC1|
          Length = 724

 Score = 28.5 bits (62), Expect = 4.2
 Identities = 25/97 (25%), Positives = 42/97 (43%)
 Frame = +2

Query: 35  IQFSTPTMLTRYIVTDRILQNHSTLLQIV*ELKRNNNIGGDTKTSHLTEEEKKSLVHSQA 214
           I  +  T+L+   +T + L N+ T + ++    R        +  ++ E  K ++    A
Sbjct: 30  IDKTVETILSYLFLTPQELLNNVTSVDLIDSPTRKGQ--SSIEAIYILEPTKYNINCIDA 87

Query: 215 PARVRPPSPRDQNPWLLPFLQTIINTHVTSQKYTAQN 325
              VRPP  R  +   LP L   I     S++Y AQN
Sbjct: 88  DFMVRPPKYRRCHIRFLPGLTNPIFQFFQSKRYIAQN 124



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>PSTC_MYCLE (Q50098) Phosphate transport system permease protein pstC|
          Length = 319

 Score = 28.1 bits (61), Expect = 5.5
 Identities = 16/57 (28%), Positives = 24/57 (42%)
 Frame = -3

Query: 297 T*VLIIVWRKGRSHGFWSLGDGGLTLAGAWE*TRLFFSSSVKWDVFVSPPMLLFLFS 127
           T  ++I+ R     G WSL DGG T A         FS  +    +++    LF  +
Sbjct: 246 TLAVLIILRSAARSGNWSLFDGGYTFASKIASAASEFSQPLPTGAYIAAGFALFFLT 302



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>AFAD_HUMAN (P55196) Afadin (AF-6 protein)|
          Length = 1816

 Score = 28.1 bits (61), Expect = 5.5
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = +2

Query: 179  EEEKKSLVHSQAPARVRPPSPRDQNP 256
            +E  + L+  +AP   RPP PRD  P
Sbjct: 1652 DEAARRLLEPEAPGLCRPPLPRDYEP 1677



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>NAD2_CAEEL (P32739) Sodium-dependent high-affinity dicarboxylate transporter 2|
           (Na(+)/dicarboxylate cotransporter 2) (NaDC-2) (ceNaDC2)
          Length = 551

 Score = 27.7 bits (60), Expect = 7.2
 Identities = 10/21 (47%), Positives = 15/21 (71%)
 Frame = -3

Query: 177 VKWDVFVSPPMLLFLFSSYTI 115
           ++W VF  PPM ++L +SY I
Sbjct: 241 LQWMVFAIPPMFVYLLASYII 261



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>AF17_HUMAN (P55198) Protein AF-17|
          Length = 1093

 Score = 27.7 bits (60), Expect = 7.2
 Identities = 12/35 (34%), Positives = 17/35 (48%)
 Frame = +2

Query: 152 GDTKTSHLTEEEKKSLVHSQAPARVRPPSPRDQNP 256
           GD K+ H+T     +  H + PA    P P D+ P
Sbjct: 425 GDYKSPHVTGSGASAGTHKRMPALSATPVPADETP 459



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>IMMB_ECOLI (P22426) Colicin-B immunity protein (Microcin-B immunity protein)|
          Length = 175

 Score = 27.3 bits (59), Expect = 9.4
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = -1

Query: 218 LEPGNEQGFFFLLLSNGMFLCLLQCYYFFSVLT 120
           LE     GFF LL  N + L +L C  +F+VLT
Sbjct: 123 LELTTAGGFFKLLSHNVITLFILYCSIYFTVLT 155



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>YKG6_CAEEL (P46556) Hypothetical protein B0285.6 in chromosome III|
          Length = 577

 Score = 27.3 bits (59), Expect = 9.4
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = -3

Query: 183 SSVKWDVFVSPPMLLFLFSSYTI 115
           S + W  F  PPM+ ++FSS+ I
Sbjct: 253 SYLSWMAFAIPPMIFYMFSSWFI 275



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>TBP_BOMMO (O45211) TATA-box-binding protein (TATA-box factor) (TATA-binding|
           factor) (TATA sequence-binding protein) (Transcription
           initiation factor TFIID TBP subunit)
          Length = 307

 Score = 27.3 bits (59), Expect = 9.4
 Identities = 11/34 (32%), Positives = 21/34 (61%)
 Frame = +2

Query: 191 KSLVHSQAPARVRPPSPRDQNPWLLPFLQTIINT 292
           + ++   +PA + P +P   +P +LP LQ I++T
Sbjct: 107 QQMLSQASPAPMTPLTPLSADPGILPQLQNIVST 140


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,860,827
Number of Sequences: 219361
Number of extensions: 1077123
Number of successful extensions: 3171
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 3097
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3171
length of database: 80,573,946
effective HSP length: 95
effective length of database: 59,734,651
effective search space used: 1433631624
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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