ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl42h01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RUBA_CHLRE (Q42694) RuBisCO large subunit-binding protein subuni... 29 2.3
2RPOC1_MAIZE (P16024) DNA-directed RNA polymerase beta' chain (EC... 28 4.0
3RPOC1_WHEAT (Q9XPS8) DNA-directed RNA polymerase beta' chain (EC... 28 6.8
4NUOH_BUCBP (Q89AU0) NADH-quinone oxidoreductase chain H (EC 1.6.... 28 6.8
5NCK2_HUMAN (O43639) Cytoplasmic protein NCK2 (NCK adaptor protei... 27 8.9

>RUBA_CHLRE (Q42694) RuBisCO large subunit-binding protein subunit alpha,|
           chloroplast precursor (60 kDa chaperonin subunit alpha)
           (CPN-60 alpha)
          Length = 580

 Score = 29.3 bits (64), Expect = 2.3
 Identities = 24/90 (26%), Positives = 47/90 (52%), Gaps = 1/90 (1%)
 Frame = -3

Query: 402 EKRANYII-KLREHSPELFKGIDDLRDVIGMNKREINNMMSGIITDSP*PS*WDGGVDGI 226
           +K A Y++ KL+EH+  + KG DD+++V  ++    +N +  +I D+         +D +
Sbjct: 154 DKTAEYLVAKLKEHAKPV-KGRDDIKNVASISAGN-DNAIGEMIADA---------LDKV 202

Query: 225 LSNFCIGRDGRMSSTTVLVVETDRESATGF 136
            SN  +  +   S+ TV+ V+   E   G+
Sbjct: 203 GSNGVLSIETSNSTETVVEVQEGMEIDRGY 232



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>RPOC1_MAIZE (P16024) DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase beta' subunit) (RNA
           polymerase beta' subunit)
          Length = 683

 Score = 28.5 bits (62), Expect = 4.0
 Identities = 19/65 (29%), Positives = 28/65 (43%)
 Frame = +1

Query: 4   LQSRKMFSLHSFIHMNHVHKIHKPPIQTPTKKQVIM***LLLLVKASGTLSICLNNKNRC 183
           L+++    L  F HMN +      PI  PT+  +I     L ++     L IC N  N C
Sbjct: 503 LEAQAEARLLMFSHMNLLSPAIGDPICVPTQDMLIG----LYVLTIGNRLGICANRYNSC 558

Query: 184 TTHPS 198
              P+
Sbjct: 559 GNSPN 563



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>RPOC1_WHEAT (Q9XPS8) DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase beta' subunit) (RNA
           polymerase beta' subunit)
          Length = 683

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 18/66 (27%), Positives = 29/66 (43%)
 Frame = +1

Query: 4   LQSRKMFSLHSFIHMNHVHKIHKPPIQTPTKKQVIM***LLLLVKASGTLSICLNNKNRC 183
           L+++    L  F HMN +      PI  PT+  +I     L ++       IC N  N C
Sbjct: 503 LEAQAEARLLMFSHMNLLSPAIGDPICVPTQDMLIG----LYVLTIGKRRGICANRYNSC 558

Query: 184 TTHPSI 201
             +P++
Sbjct: 559 RNYPNL 564



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>NUOH_BUCBP (Q89AU0) NADH-quinone oxidoreductase chain H (EC 1.6.99.5) (NADH|
           dehydrogenase I, chain H) (NDH-1, chain H)
          Length = 322

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = -1

Query: 110 IITCFFVGVWIGGLCILWTWFI*MNECSENIFLL 9
           +I+  F G W+G L   + WFI    C   IF+L
Sbjct: 251 LISTMFFGGWLGPLFPSYFWFILKTLCFMMIFIL 284



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>NCK2_HUMAN (O43639) Cytoplasmic protein NCK2 (NCK adaptor protein 2) (SH2/SH3|
           adaptor protein NCK-beta) (Nck-2)
          Length = 380

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 12/30 (40%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
 Frame = +1

Query: 262 GAVGDDPRHHIIYL---PFVHPNHIPEIVY 342
           G VG  P+++++ L   P +HP H P+I Y
Sbjct: 242 GQVGLVPKNYVVVLSDGPALHPAHAPQISY 271


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,627,686
Number of Sequences: 219361
Number of extensions: 1172308
Number of successful extensions: 2865
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2793
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2863
length of database: 80,573,946
effective HSP length: 111
effective length of database: 56,224,875
effective search space used: 1349397000
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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