ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl42c08
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AMPN_ECOLI (P04825) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoa... 90 3e-18
2AMPN_CAUCR (P37893) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoa... 77 1e-14
3AMPN_HAEIN (P45274) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoa... 75 1e-13
4AMP1_PLAFQ (O96935) M1-family aminopeptidase (EC 3.4.11.-) (Pfa-M1) 58 9e-09
5KEX1_YEAST (P09620) Carboxypeptidase KEX1 precursor (EC 3.4.16.6... 29 4.6
6RPOM_HUMAN (O00411) DNA-directed RNA polymerase, mitochondrial p... 29 4.6
7HEM1_HALSA (Q9HP72) Glutamyl-tRNA reductase (EC 1.2.1.70) (GluTR) 29 6.0

>AMPN_ECOLI (P04825) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide|
            hydrolase)
          Length = 869

 Score = 89.7 bits (221), Expect = 3e-18
 Identities = 47/97 (48%), Positives = 63/97 (64%), Gaps = 1/97 (1%)
 Frame = -1

Query: 447  FYMRNPNKVYSLIGGFCGS-PVSFHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRW 271
            F M NPN++ SLIG F GS P +FHA+DGSGY FL E++  L+  NPQVASR++    R 
Sbjct: 773  FTMSNPNRIRSLIGAFAGSNPAAFHAEDGSGYLFLVEMLTDLNSRNPQVASRLIEPLIRL 832

Query: 270  RRYDETRQALAKAQLEMIISANGLSENVYGIASKSLA 160
            +RYD  RQ   +A LE +     LS ++Y   +K+LA
Sbjct: 833  KRYDAKRQEKMRAALEQLKGLENLSGDLYEKITKALA 869



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>AMPN_CAUCR (P37893) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide|
            hydrolase)
          Length = 863

 Score = 77.4 bits (189), Expect = 1e-14
 Identities = 39/96 (40%), Positives = 57/96 (59%), Gaps = 1/96 (1%)
 Frame = -1

Query: 447  FYMRNPNKVYSLIGGFCG-SPVSFHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRW 271
            F   NPN++ +L+  F   +P  FH   G+GY FL + +L++D  NP  A+R+V     W
Sbjct: 767  FEPTNPNRLRALVSTFANFNPARFHDPSGAGYAFLADEILKVDAFNPMTAARLVEPLGGW 826

Query: 270  RRYDETRQALAKAQLEMIISANGLSENVYGIASKSL 163
            RRY      L +AQLE I++   LS+NV  +ASK+L
Sbjct: 827  RRYKPELGDLMRAQLERIVAHPNLSKNVLELASKAL 862



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>AMPN_HAEIN (P45274) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide|
            hydrolase)
          Length = 869

 Score = 74.7 bits (182), Expect = 1e-13
 Identities = 37/96 (38%), Positives = 60/96 (62%), Gaps = 1/96 (1%)
 Frame = -1

Query: 447  FYMRNPNKVYSLIGGFCGSPV-SFHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRW 271
            F   NPN++ SL+G F    + +FH   GSGY+FL +V+++L++ NPQVA+R++    R+
Sbjct: 773  FNFNNPNRLRSLVGSFANHNLKAFHNVSGSGYRFLTDVLIRLNESNPQVAARLIEPLIRF 832

Query: 270  RRYDETRQALAKAQLEMIISANGLSENVYGIASKSL 163
             R+D  RQ L K  LE +     LS++++    K+L
Sbjct: 833  SRFDAQRQTLMKRALERLSVVENLSKDLFEKIEKAL 868



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>AMP1_PLAFQ (O96935) M1-family aminopeptidase (EC 3.4.11.-) (Pfa-M1)|
          Length = 1085

 Score = 58.2 bits (139), Expect = 9e-09
 Identities = 26/84 (30%), Positives = 44/84 (52%)
 Frame = -1

Query: 438  RNPNKVYSLIGGFCGSPVSFHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRWRRYD 259
            +NPN + ++   F  +   FH   G GYK + EV+ + DK NP VA+++   F  W + D
Sbjct: 991  KNPNDIRAVYLPFTNNLRRFHDISGKGYKLIAEVITKTDKFNPMVATQLCEPFKLWNKLD 1050

Query: 258  ETRQALAKAQLEMIISANGLSENV 187
              RQ L   ++  ++    +S N+
Sbjct: 1051 TKRQELMLNEMNTMLQEPNISNNL 1074



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>KEX1_YEAST (P09620) Carboxypeptidase KEX1 precursor (EC 3.4.16.6)|
           (Carboxypeptidase D)
          Length = 729

 Score = 29.3 bits (64), Expect = 4.6
 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 4/57 (7%)
 Frame = +3

Query: 48  HAKYSRIYSVKYTFKALVMVKG----STGKIKFVSLHQETKLPDFSRLSHRHFQRVH 206
           H K+S+I    Y  KAL++  G    +T  + ++    E KL D S  + +H    H
Sbjct: 214 HNKFSKIDGDTYDLKALLIGNGWIDPNTQSLSYLPFAMEKKLIDESNPNFKHLTNAH 270



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>RPOM_HUMAN (O00411) DNA-directed RNA polymerase, mitochondrial precursor (EC|
            2.7.7.6) (MtRPOL)
          Length = 1230

 Score = 29.3 bits (64), Expect = 4.6
 Identities = 17/57 (29%), Positives = 25/57 (43%)
 Frame = +3

Query: 24   TIVACYFTHAKYSRIYSVKYTFKALVMVKGSTGKIKFVSLHQETKLPDFSRLSHRHF 194
            T + CY     +  ++   +T  A V V     + +FV LH E  L D SR   + F
Sbjct: 1135 TALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRF 1191



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>HEM1_HALSA (Q9HP72) Glutamyl-tRNA reductase (EC 1.2.1.70) (GluTR)|
          Length = 436

 Score = 28.9 bits (63), Expect = 6.0
 Identities = 21/65 (32%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
 Frame = +2

Query: 191 FSESPLAEIIISNWALARACLVSSYLLHRENADTMRDAT*GLILSSCKTT---SPKNL*P 361
           F+ + +AEI+I+N   ARA  V+  +     A T+ DA   L  +    T   SP+++ P
Sbjct: 196 FASATVAEIVIANRTPARAVRVADMVSVPAEATTLADARGRLDAADVVVTATGSPEHVLP 255

Query: 362 EPSFA 376
             +FA
Sbjct: 256 ASAFA 260


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,044,181
Number of Sequences: 219361
Number of extensions: 1301252
Number of successful extensions: 2962
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 2903
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2960
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2677159704
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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