ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl41e03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1PUB2_SCHPO (Q9UTG2) E3 ubiquitin--protein ligase pub2 (EC 6.3.2.-) 30 1.5
2YCF2_MARPO (P09975) Protein ycf2 30 1.5
3ULA1_SCHPO (Q9UT93) NEDD8-activating enzyme E1 regulatory subuni... 29 2.5
4Y527_MYCPN (P75251) Hypothetical protein MG350.1 homolog (G12_or... 29 2.5
5CYAA_SACKL (P23466) Adenylate cyclase (EC 4.6.1.1) (ATP pyrophos... 28 5.6
6RL6_BORBU (O51446) 50S ribosomal protein L6 28 5.6
7PPCK_RHOPA (Q9ZNH4) Phosphoenolpyruvate carboxykinase [ATP] (EC ... 28 7.3
8CHD9_HUMAN (Q3L8U1) Chromodomain-helicase-DNA-binding protein 9 ... 28 7.3
9UBIQ_LEITA (P49635) Ubiquitin 27 9.5
10ACVS_STRCL (Q01757) N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D... 27 9.5
11MRGRH_RAT (Q7TN38) Mas-related G-protein coupled receptor member H 27 9.5

>PUB2_SCHPO (Q9UTG2) E3 ubiquitin--protein ligase pub2 (EC 6.3.2.-)|
          Length = 671

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 19/65 (29%), Positives = 36/65 (55%), Gaps = 1/65 (1%)
 Frame = -3

Query: 297 YAKVEGLVPERCRNDFPKFRKHTEQKGTTEDNIEKESLKHMILIRRGKLNLQV-EYVSIR 121
           + K+E L P++ +  F +F  + + K + + N+E + +KH  L+    L L V + V++ 
Sbjct: 183 HEKLENLTPKQLKEVFSQFLFNNQSKSSLKINLEYKVIKH--LLEHYPLALSVRQQVAVE 240

Query: 120 K*LLP 106
           K  LP
Sbjct: 241 KGPLP 245



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>YCF2_MARPO (P09975) Protein ycf2|
          Length = 2136

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 13/42 (30%), Positives = 27/42 (64%), Gaps = 1/42 (2%)
 Frame = +2

Query: 128  LTYSTCKFNFPRLIRIICFRLSFSILSSV-VPFCSVCFLNLG 250
            LTY+  K NF R+ +I+ +++  +I+ ++ +   S+  LN+G
Sbjct: 1623 LTYTNNKLNFDRIFKIVIYKVGKTIIQNILIKSSSMNLLNIG 1664



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>ULA1_SCHPO (Q9UT93) NEDD8-activating enzyme E1 regulatory subunit|
           (Ubiquitin-like activation protein 1)
           (Ubiquitin-activating enzyme E1-like 1)
          Length = 500

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 4/61 (6%)
 Frame = -3

Query: 309 SRDQYAKVEGLVPERCRNDFPKFRKHTEQK----GTTEDNIEKESLKHMILIRRGKLNLQ 142
           S  QY K++ +  E+  ND  KF+K+ +Q       + + I    +KH     R  LN++
Sbjct: 322 STQQYVKLQVIYKEKSENDILKFKKYVQQTLKRLNRSVEEITDLEIKH---FSRNCLNIK 378

Query: 141 V 139
           V
Sbjct: 379 V 379



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>Y527_MYCPN (P75251) Hypothetical protein MG350.1 homolog (G12_orf225)|
          Length = 225

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 17/58 (29%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
 Frame = +2

Query: 44  ISQQWYKILTVSFVICKITLGGNSYFLILTYSTCKFNFPRLIRIICF-RLSFSILSSV 214
           +S  W  ++TV+  +C     GN++  ILT +   F       I+ F RL F I + +
Sbjct: 66  VSYSWALVITVALSLCSFIWDGNNWIGILTLTIANF------AIVSFTRLYFHIFAQI 117



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>CYAA_SACKL (P23466) Adenylate cyclase (EC 4.6.1.1) (ATP pyrophosphate-lyase)|
           (Adenylyl cyclase)
          Length = 1839

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 13/39 (33%), Positives = 20/39 (51%)
 Frame = -3

Query: 306 RDQYAKVEGLVPERCRNDFPKFRKHTEQKGTTEDNIEKE 190
           R+++ ++E    E   N  PKF+KH     TT  N E +
Sbjct: 14  REKHPQIEETFEEHVHNAMPKFKKHYALGITTHTNDEDD 52



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>RL6_BORBU (O51446) 50S ribosomal protein L6|
          Length = 180

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 13/37 (35%), Positives = 24/37 (64%)
 Frame = +2

Query: 32  LVQNISQQWYKILTVSFVICKITLGGNSYFLILTYST 142
           +V+ +++ + K LT++ +  ++   GNS FL L YST
Sbjct: 75  MVKGVTEGFSKSLTINGIGYRVEQQGNSLFLSLGYST 111



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>PPCK_RHOPA (Q9ZNH4) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP|
           carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK)
          Length = 537

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 12/36 (33%), Positives = 19/36 (52%)
 Frame = -3

Query: 306 RDQYAKVEGLVPERCRNDFPKFRKHTEQKGTTEDNI 199
           R + A++E  VPE    D P FR   ++ G   +N+
Sbjct: 143 RPERAELENFVPELTLIDLPSFRADPKRHGCRSENV 178



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>CHD9_HUMAN (Q3L8U1) Chromodomain-helicase-DNA-binding protein 9 (EC 3.6.1.-)|
            (ATP-dependent helicase CHD9) (CHD-9) (Chromatin-related
            mesenchymal modulator) (CReMM) (Chromatin remodeling
            factor CHROM1) (Peroxisomal proliferator-activated
            receptor A-inter
          Length = 2897

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 18/62 (29%), Positives = 31/62 (50%)
 Frame = -3

Query: 273  PERCRNDFPKFRKHTEQKGTTEDNIEKESLKHMILIRRGKLNLQVEYVSIRK*LLPPNVI 94
            P+R + D+ KFR        T+D++EK     M + RR      V  +  ++ L+ PN+ 
Sbjct: 1859 PDRGQFDWTKFRAMARLHKKTDDSLEKYLYAFMSMCRR------VCRLPSKEELVDPNIF 1912

Query: 93   LQ 88
            +Q
Sbjct: 1913 IQ 1914



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>UBIQ_LEITA (P49635) Ubiquitin|
          Length = 76

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 18/44 (40%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
 Frame = -3

Query: 285 EGLVPERCRNDFPKFRKHTEQKGTTED-NIEKESLKHMILIRRG 157
           EG+ P++ R  F    K  E+  T  D NI+KES  H++L  RG
Sbjct: 34  EGIPPDQQRLIFAD--KQLEEGRTLSDYNIQKESTLHLVLRLRG 75



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>ACVS_STRCL (Q01757) N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine|
           synthase (EC 6.3.2.26)
           (Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine
           synthetase) (ACV synthetase) (ACVS) (Fragment)
          Length = 805

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 16/43 (37%), Positives = 21/43 (48%)
 Frame = -3

Query: 279 LVPERCRNDFPKFRKHTEQKGTTEDNIEKESLKHMILIRRGKL 151
           +VPE  R D  +F  + E+ G T  N     L+H  L RR  L
Sbjct: 490 VVPEEVRLDPDRFPAYIEEHGVTYLNATGSVLQHFDLRRRTSL 532



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>MRGRH_RAT (Q7TN38) Mas-related G-protein coupled receptor member H|
          Length = 321

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 27/105 (25%), Positives = 42/105 (40%), Gaps = 25/105 (23%)
 Frame = +2

Query: 11  CDQICTQLVQNISQQ---W-------YKILTVSFVICKITLGGNS---YFLILTYSTCKF 151
           C Q CTQ  +N +     W       Y  +++S VIC + L GN    +FLI       F
Sbjct: 9   CPQECTQTTRNETPNETTWSSEHVTKYTYISISLVICSLGLVGNGLLIWFLIFCIKRKPF 68

Query: 152 N-------FPRLIRIICFRL-----SFSILSSVVPFCSVCFLNLG 250
                   F   + ++C  +     +F I  S +   +V F+  G
Sbjct: 69  TIYILHLAFADFMVLLCSSIIQLVNTFHIYDSTLVSYAVLFMIFG 113


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,838,487
Number of Sequences: 219361
Number of extensions: 807965
Number of successful extensions: 1902
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1887
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1902
length of database: 80,573,946
effective HSP length: 91
effective length of database: 60,612,095
effective search space used: 1454690280
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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