ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl38f06
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TBCD_MOUSE (Q8BYA0) Tubulin-specific chaperone D (Tubulin-foldin... 58 8e-09
2TBCD_HUMAN (Q9BTW9) Tubulin-specific chaperone D (Tubulin-foldin... 54 1e-07
3TBCD_BOVIN (Q28205) Tubulin-specific chaperone D (Tubulin-foldin... 44 1e-04
4SEC2_YEAST (P17065) Rab guanine nucleotide exchange factor SEC2 ... 33 0.22
5FANCG_HUMAN (O15287) Fanconi anemia group G protein (Protein FAC... 32 0.83
6IF2B_RABIT (P41035) Eukaryotic translation initiation factor 2 s... 31 1.1
7UBP8_MOUSE (Q80U87) Ubiquitin carboxyl-terminal hydrolase 8 (EC ... 31 1.4
8IF2B_HUMAN (P20042) Eukaryotic translation initiation factor 2 s... 31 1.4
9CP7A1_CRIGR (P46634) Cytochrome P450 7A1 (EC 1.14.13.17) (Choles... 30 2.4
10CP7A1_RAT (P18125) Cytochrome P450 7A1 (EC 1.14.13.17) (Choleste... 29 4.1
11PKSL_BACSU (Q05470) Putative polyketide synthase pksL (PKS) 29 5.4
12RDRP_PEAMV (P29154) RNA-directed RNA polymerase (EC 2.7.7.48) 29 5.4
13ARBB_ERWCH (P26206) 6-phospho-beta-glucosidase (EC 3.2.1.86) 28 7.1
14RIMS1_HUMAN (Q86UR5) Regulating synaptic membrane exocytosis pro... 28 9.2
15BUD3_YEAST (P25558) Bud site selection protein BUD3 28 9.2

>TBCD_MOUSE (Q8BYA0) Tubulin-specific chaperone D (Tubulin-folding cofactor D)|
            (Beta-tubulin cofactor D)
          Length = 1196

 Score = 58.2 bits (139), Expect = 8e-09
 Identities = 27/72 (37%), Positives = 43/72 (59%)
 Frame = -1

Query: 414  KAFSQLLVFLGHRYPKIRKAAADQVYLVLLQNDSLISAEDMDKAQEVLAETCWEGDVEEA 235
            K   QL + LGH +P IRK+ A QVY ++L    L+ AE +D+   VL++T W+ ++   
Sbjct: 1111 KILLQLFLLLGHPFPVIRKSTASQVYEMVLTYSDLVDAEVLDEVMSVLSDTAWDAELPVV 1170

Query: 234  RHKRSELNVMAG 199
            R +R+ L  + G
Sbjct: 1171 REQRNRLCDLLG 1182



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>TBCD_HUMAN (Q9BTW9) Tubulin-specific chaperone D (Tubulin-folding cofactor D)|
            (Beta-tubulin cofactor D) (tfcD) (SSD-1)
          Length = 1192

 Score = 54.3 bits (129), Expect = 1e-07
 Identities = 26/72 (36%), Positives = 42/72 (58%)
 Frame = -1

Query: 414  KAFSQLLVFLGHRYPKIRKAAADQVYLVLLQNDSLISAEDMDKAQEVLAETCWEGDVEEA 235
            +A  QL + L HR+P IRK  A QVY  LL    ++ A+ +D+   VL++T W+ ++   
Sbjct: 1108 QALLQLCLLLCHRFPLIRKTTASQVYETLLTYSDVVGADVLDEVVTVLSDTAWDAELAVV 1167

Query: 234  RHKRSELNVMAG 199
            R +R+ L  + G
Sbjct: 1168 REQRNRLCDLLG 1179



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>TBCD_BOVIN (Q28205) Tubulin-specific chaperone D (Tubulin-folding cofactor D)|
            (Beta-tubulin cofactor D)
          Length = 1199

 Score = 44.3 bits (103), Expect = 1e-04
 Identities = 24/72 (33%), Positives = 39/72 (54%)
 Frame = -1

Query: 414  KAFSQLLVFLGHRYPKIRKAAADQVYLVLLQNDSLISAEDMDKAQEVLAETCWEGDVEEA 235
            K   QL + L H +P IRK  A QVY ++L  D ++    +D+   VL+ T W+ ++   
Sbjct: 1116 KVLLQLFLLLCHPFPVIRKNTASQVYEMVLTYD-VVPTAVLDEVMAVLSSTAWDAELPVV 1174

Query: 234  RHKRSELNVMAG 199
            R +R+ L  + G
Sbjct: 1175 RAQRNRLCDLLG 1186



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>SEC2_YEAST (P17065) Rab guanine nucleotide exchange factor SEC2 (GDP-GTP|
           exchange factor SEC2)
          Length = 759

 Score = 33.5 bits (75), Expect = 0.22
 Identities = 17/47 (36%), Positives = 26/47 (55%)
 Frame = -1

Query: 309 ISAEDMDKAQEVLAETCWEGDVEEARHKRSELNVMAGFGVAASDKSE 169
           I+ ED++K  EV   T  +G V     K   L+ MAG G+ +S K++
Sbjct: 408 ITKEDLEKFSEVTKHTKRDGRVSSQDKKTKRLSFMAGLGINSSTKNK 454



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>FANCG_HUMAN (O15287) Fanconi anemia group G protein (Protein FACG) (DNA-repair|
           protein XRCC9)
          Length = 622

 Score = 31.6 bits (70), Expect = 0.83
 Identities = 21/61 (34%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
 Frame = +3

Query: 51  GRTTAPLLSIH*IQPARNTTCSRQSKQHFEHRELLLPVDSQTCPRR-RRQNQPSHSVPTS 227
           G TTA L S+  +  A N  CS ++ Q     ELLLP      P     Q+Q  H + + 
Sbjct: 294 GDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHILASR 353

Query: 228 C 230
           C
Sbjct: 354 C 354



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>IF2B_RABIT (P41035) Eukaryotic translation initiation factor 2 subunit 2|
           (Eukaryotic translation initiation factor 2 beta
           subunit) (eIF-2-beta)
          Length = 333

 Score = 31.2 bits (69), Expect = 1.1
 Identities = 22/89 (24%), Positives = 44/89 (49%), Gaps = 5/89 (5%)
 Frame = -1

Query: 297 DMDKAQEVLAETCWEGDVEEARHKRSELNVMAGFGVAASDKSENRQGARAPD-----VRN 133
           D+D+A+E + +   E DV+E      +L++M G      +K + ++  + PD      ++
Sbjct: 90  DIDEAEEGVKDLKIENDVQEPAEPEDDLDIMLG------NKKKKKKNVKFPDEDEILEKD 143

Query: 132 AASTDENTSYSSLVEFSGY*AGGPSFSPS 46
            A  DE++     + FS     GP+++ S
Sbjct: 144 EALEDEDSKKDDGISFSN--QTGPAWAGS 170



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>UBP8_MOUSE (Q80U87) Ubiquitin carboxyl-terminal hydrolase 8 (EC 3.1.2.15)|
           (Ubiquitin thioesterase 8)
           (Ubiquitin-specific-processing protease 8)
           (Deubiquitinating enzyme 8) (mUBPy)
          Length = 1080

 Score = 30.8 bits (68), Expect = 1.4
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = +3

Query: 105 TTCSRQSKQHFEHRELLLPVDSQTCPRRRRQNQPSHSVPTS 227
           T   RQSK   E  +  +PV+ + CP    Q +P+   P S
Sbjct: 543 TGTQRQSKSEHEASDAKVPVEGKRCPTSEAQKRPADVSPAS 583



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>IF2B_HUMAN (P20042) Eukaryotic translation initiation factor 2 subunit 2|
           (Eukaryotic translation initiation factor 2 beta
           subunit) (eIF-2-beta)
          Length = 333

 Score = 30.8 bits (68), Expect = 1.4
 Identities = 22/89 (24%), Positives = 43/89 (48%), Gaps = 5/89 (5%)
 Frame = -1

Query: 297 DMDKAQEVLAETCWEGDVEEARHKRSELNVMAGFGVAASDKSENRQGARAPD-----VRN 133
           D+D+A+E + +   E DV+E      +L++M G      +K + ++  + PD      ++
Sbjct: 90  DIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLG------NKKKKKKNVKFPDEDEILEKD 143

Query: 132 AASTDENTSYSSLVEFSGY*AGGPSFSPS 46
            A  DE+      + FS     GP+++ S
Sbjct: 144 EALEDEDNKKDDGISFSN--QTGPAWAGS 170



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>CP7A1_CRIGR (P46634) Cytochrome P450 7A1 (EC 1.14.13.17) (Cholesterol|
           7-alpha-monooxygenase) (CYPVII) (Cholesterol
           7-alpha-hydroxylase)
          Length = 504

 Score = 30.0 bits (66), Expect = 2.4
 Identities = 17/49 (34%), Positives = 27/49 (55%)
 Frame = -1

Query: 414 KAFSQLLVFLGHRYPKIRKAAADQVYLVLLQNDSLISAEDMDKAQEVLA 268
           KA  QL   L H    +R   ++ + L +  ND+L + +DM+KA+  LA
Sbjct: 233 KAREQLAEGLKHENLSVRDQVSELIRLRMFLNDTLSTFDDMEKAKTHLA 281



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>CP7A1_RAT (P18125) Cytochrome P450 7A1 (EC 1.14.13.17) (Cholesterol|
           7-alpha-monooxygenase) (CYPVII) (Cholesterol
           7-alpha-hydroxylase)
          Length = 503

 Score = 29.3 bits (64), Expect = 4.1
 Identities = 17/52 (32%), Positives = 29/52 (55%)
 Frame = -1

Query: 423 TGTKAFSQLLVFLGHRYPKIRKAAADQVYLVLLQNDSLISAEDMDKAQEVLA 268
           T  KA  +L   L H+   +R   ++ + L +  ND+L + +DM+KA+  LA
Sbjct: 230 TAHKAREKLAEGLKHKNLCVRDQVSELIRLRMFLNDTLSTFDDMEKAKTHLA 281



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>PKSL_BACSU (Q05470) Putative polyketide synthase pksL (PKS)|
          Length = 4538

 Score = 28.9 bits (63), Expect = 5.4
 Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 12/47 (25%)
 Frame = -1

Query: 303 AEDMDKAQEVLAETCWEGDVEEA------------RHKRSELNVMAG 199
           AE MD  + +  ETCWE  +E+A            R+KR  + V AG
Sbjct: 514 AETMDPQERLFLETCWE-TIEDAGYTPKTLAKPKGRNKRQHVGVFAG 559



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>RDRP_PEAMV (P29154) RNA-directed RNA polymerase (EC 2.7.7.48)|
          Length = 599

 Score = 28.9 bits (63), Expect = 5.4
 Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
 Frame = -1

Query: 246 VEEARHKRSELNVMAGFGVAAS-DKSENRQGARAPDVRNAASTDEN 112
           V+    K SE  +  G GV+++  +S++R+G   P  RN A T+E+
Sbjct: 13  VDSGETKSSEDPLPKGRGVSSTPSRSKSRKGKACPSFRNDAGTEES 58



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>ARBB_ERWCH (P26206) 6-phospho-beta-glucosidase (EC 3.2.1.86)|
          Length = 465

 Score = 28.5 bits (62), Expect = 7.1
 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
 Frame = -1

Query: 339 YLVLLQNDSLISA-EDMDKAQEVLAETCWEGDVEEARHKRSELNVMAGF 196
           Y +   ND L+   E +D   EVL  TCW G ++     ++E++   GF
Sbjct: 381 YRIRYLNDHLVQVGEAIDDGVEVLGYTCW-GPIDLVSASKAEMSKRYGF 428



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>RIMS1_HUMAN (Q86UR5) Regulating synaptic membrane exocytosis protein 1|
           (Rab3-interacting molecule 1) (RIM 1)
          Length = 1692

 Score = 28.1 bits (61), Expect = 9.2
 Identities = 16/50 (32%), Positives = 23/50 (46%)
 Frame = -1

Query: 246 VEEARHKRSELNVMAGFGVAASDKSENRQGARAPDVRNAASTDENTSYSS 97
           V  ARH+R   +V      A +   E + G RAP    A+  D   +YS+
Sbjct: 381 VSRARHERRHSDVALPRTEAGAALPEGKAGKRAPAAARASPPDSPRAYSA 430



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>BUD3_YEAST (P25558) Bud site selection protein BUD3|
          Length = 1636

 Score = 28.1 bits (61), Expect = 9.2
 Identities = 18/44 (40%), Positives = 24/44 (54%)
 Frame = -1

Query: 189  AASDKSENRQGARAPDVRNAASTDENTSYSSLVEFSGY*AGGPS 58
            A   KS++  G + PDV N A   ENT  SS+V  + Y +  PS
Sbjct: 928  AVEFKSDDLIG-KPPDVGNGAHPQENTRISSVVRDTKYVSYNPS 970


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,433,548
Number of Sequences: 219361
Number of extensions: 1218417
Number of successful extensions: 3817
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 3599
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3816
length of database: 80,573,946
effective HSP length: 101
effective length of database: 58,418,485
effective search space used: 2395157885
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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