ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl38d07
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1EPIPL_MOUSE (Q8R0W0) Epiplakin 30 1.4
2KI18A_MOUSE (Q91WD7) Kinesin family member 18A 30 1.8
3KI18A_HUMAN (Q8NI77) Kinesin family member 18A 30 1.8
4ACOT4_HUMAN (Q8N9L9) Acyl-coenzyme A thioesterase 4 (EC 3.1.2.2)... 29 2.3
5PLEC1_CRIGR (Q9JI55) Plectin-1 (PLTN) (PCN) (300-kDa intermediat... 28 4.0
6NOS3_BOVIN (P29473) Nitric-oxide synthase, endothelial (EC 1.14.... 28 4.0
7DHAS_METTH (O26890) Aspartate-semialdehyde dehydrogenase (EC 1.2... 28 5.2
8DHMH_PARDE (P29894) Methylamine dehydrogenase heavy chain precur... 28 5.2
9SEF1_YEAST (P34228) Suppressor protein SEF1 28 6.8
10DAPF_SYNY3 (P74667) Diaminopimelate epimerase (EC 5.1.1.7) (DAP ... 28 6.8
11SYN_UREPA (Q9PQC6) Asparaginyl-tRNA synthetase (EC 6.1.1.22) (As... 28 6.8
12PUR8_SYNY3 (P74384) Adenylosuccinate lyase (EC 4.3.2.2) (Adenylo... 28 6.8
13ACOT2_HUMAN (P49753) Acyl-coenzyme A thioesterase 2 (EC 3.1.2.2)... 28 6.8

>EPIPL_MOUSE (Q8R0W0) Epiplakin|
          Length = 6548

 Score = 30.0 bits (66), Expect = 1.4
 Identities = 16/48 (33%), Positives = 25/48 (52%)
 Frame = -1

Query: 350  EEQLDRCEEGRMVVAAVRREEDATRYAAMMSKAAGIGREICGDGAKCE 207
            ++ L+  EEGR  V  + R+ED  RY    S  AG+   + G+  + E
Sbjct: 2202 KQTLEDLEEGRTTVDQIERKEDVKRYLKGTSCIAGVLVPVQGEPGRQE 2249



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>KI18A_MOUSE (Q91WD7) Kinesin family member 18A|
          Length = 886

 Score = 29.6 bits (65), Expect = 1.8
 Identities = 14/44 (31%), Positives = 23/44 (52%)
 Frame = +3

Query: 69  TIAHLAAVQHVHCRPSSTNTYHLKVIICHRHRRHFLPDPVQLQH 200
           T   L  +Q + C  S ++   LK+    R RR  +P P+++QH
Sbjct: 637 TFPQLEPIQSISCCTSVSDPNVLKLTPQRRTRRKIIPSPLKVQH 680



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>KI18A_HUMAN (Q8NI77) Kinesin family member 18A|
          Length = 898

 Score = 29.6 bits (65), Expect = 1.8
 Identities = 16/44 (36%), Positives = 22/44 (50%)
 Frame = +3

Query: 69  TIAHLAAVQHVHCRPSSTNTYHLKVIICHRHRRHFLPDPVQLQH 200
           T   L  VQ + C  SS  T  +K+    R RR  +P P++ QH
Sbjct: 637 TFPQLGPVQPIPCCSSSGGTNLVKIPTEKRTRRKLMPSPLKGQH 680



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>ACOT4_HUMAN (Q8N9L9) Acyl-coenzyme A thioesterase 4 (EC 3.1.2.2) (Acyl-CoA|
           thioesterase 4) (Peroxisomal acyl coenzyme A thioester
           hydrolase Ib) (Peroxisomal long-chain acyl-coA
           thioesterase Ib) (PTE-Ib) (PTE-2b)
          Length = 421

 Score = 29.3 bits (64), Expect = 2.3
 Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
 Frame = +3

Query: 144 IICH-RHRRHFLPDPVQLQHERLAFGAVPADLPPDPG 251
           ++C  +H RHFLP  V+ Q  R         LPP PG
Sbjct: 119 LLCQAQHERHFLPPGVRRQSVRAGRVRATLFLPPGPG 155



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>PLEC1_CRIGR (Q9JI55) Plectin-1 (PLTN) (PCN) (300-kDa intermediate|
            filament-associated protein) (IFAP300) (Fragment)
          Length = 4473

 Score = 28.5 bits (62), Expect = 4.0
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = -1

Query: 347  EQLDRCEEGRMVVAAVRREEDATRYAAMMSKAAGI 243
            E+L R E+G   VA + + ED  +Y    S  AG+
Sbjct: 2593 EELQRLEQGHTTVAELSQREDVRQYLQGRSSIAGL 2627



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>NOS3_BOVIN (P29473) Nitric-oxide synthase, endothelial (EC 1.14.13.39)|
           (EC-NOS) (NOS type III) (NOSIII) (Endothelial NOS)
           (eNOS) (Constitutive NOS) (cNOS)
          Length = 1204

 Score = 28.5 bits (62), Expect = 4.0
 Identities = 14/36 (38%), Positives = 20/36 (55%)
 Frame = +3

Query: 33  MKSYTSSSFRARTIAHLAAVQHVHCRPSSTNTYHLK 140
           +  Y SS  R+ + AH   +Q V    +ST TYHL+
Sbjct: 132 INQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLR 167



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>DHAS_METTH (O26890) Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) (ASA|
           dehydrogenase) (ASADH)
          Length = 347

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 14/42 (33%), Positives = 22/42 (52%)
 Frame = -1

Query: 407 GIDEIPEKYIMARWTDGCREEQLDRCEEGRMVVAAVRREEDA 282
           G+   PEK ++ R  +   + ++DR  +G M V   R  EDA
Sbjct: 272 GLHSAPEKPVVVRDEENRPQPRMDRDMDGGMAVTVGRLREDA 313



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>DHMH_PARDE (P29894) Methylamine dehydrogenase heavy chain precursor (EC|
           1.4.99.3) (MADH)
          Length = 417

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 16/46 (34%), Positives = 23/46 (50%), Gaps = 5/46 (10%)
 Frame = -2

Query: 169 WRR*RWQMITF-----RWYVLVELGRQWTC*TAARCAIVLARKEEE 47
           WR   WQ + +     R Y+LV+   +W   TA+R  +VL  K  E
Sbjct: 308 WRPGGWQQVAYHRALDRIYLLVDQRDEWRHKTASRLLVVLDAKTGE 353



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>SEF1_YEAST (P34228) Suppressor protein SEF1|
          Length = 1057

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 13/38 (34%), Positives = 18/38 (47%), Gaps = 4/38 (10%)
 Frame = -3

Query: 129 TCWLSLGDSGHVEQQQDVLLFWPGRRRK----CSSSFI 28
           TCW+    S H+ Q   +L  WP   +K    CS  F+
Sbjct: 413 TCWIRTPRSTHISQALLILCIWPLPNQKVLDDCSYRFV 450



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>DAPF_SYNY3 (P74667) Diaminopimelate epimerase (EC 5.1.1.7) (DAP epimerase)|
          Length = 279

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = -1

Query: 290 EDATRYAAMMSKAAGIGREICGDGAKCEA-FMLELDRIREK 171
           +  T Y   +  + G   E+CG+G +C A F+ +L+ + EK
Sbjct: 55  QGGTDYTMRIFNSDGSEPEMCGNGIRCLAKFLADLEGVEEK 95



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>SYN_UREPA (Q9PQC6) Asparaginyl-tRNA synthetase (EC 6.1.1.22)|
           (Asparagine--tRNA ligase) (AsnRS)
          Length = 454

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 14/39 (35%), Positives = 22/39 (56%)
 Frame = -1

Query: 350 EEQLDRCEEGRMVVAAVRREEDATRYAAMMSKAAGIGRE 234
           E+ L RC+E +M + +++   D  R+   MS   GIG E
Sbjct: 389 EKLLKRCQELKMPIESLQWYLDLRRFGYYMSSGFGIGFE 427



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>PUR8_SYNY3 (P74384) Adenylosuccinate lyase (EC 4.3.2.2) (Adenylosuccinase)|
           (ASL)
          Length = 431

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 14/60 (23%), Positives = 30/60 (50%)
 Frame = -1

Query: 329 EEGRMVVAAVRREEDATRYAAMMSKAAGIGREICGDGAKCEAFMLELDRIREKMATMTMA 150
           E+   ++ A+R +    RY  M+ ++ GI  E    G K   ++ E+ R R+++  +  +
Sbjct: 115 EQLEKLIQAIRYQAQQHRYTVMVGRSHGIHAEPITFGFKLAGWLAEVLRNRDRLVRVAQS 174



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>ACOT2_HUMAN (P49753) Acyl-coenzyme A thioesterase 2 (EC 3.1.2.2) (Acyl-CoA|
           thioesterase 2) (Peroxisomal acyl-coenzyme A thioester
           hydrolase 2a) (Peroxisomal long-chain acyl-coA
           thioesterase 2) (ZAP128) (CTE-Ia)
          Length = 483

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
 Frame = +3

Query: 144 IICH-RHRRHFLPDPVQLQHERLAFGAVPADLPPDPG 251
           ++C  RH R+FLP  V+ +  R+        LPP+PG
Sbjct: 181 LLCQTRHERYFLPPGVRREPVRVGRVRGTLFLPPEPG 217


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,661,329
Number of Sequences: 219361
Number of extensions: 701066
Number of successful extensions: 2033
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 2007
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2033
length of database: 80,573,946
effective HSP length: 112
effective length of database: 56,005,514
effective search space used: 1344132336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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