ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl32e05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SPSE_BACSU (P39625) Spore coat polysaccharide biosynthesis prote... 32 0.80
2FLGE_BORBU (Q44767) Flagellar hook protein flgE 30 4.0
3IF2_LEGPL (Q5WT36) Translation initiation factor IF-2 30 4.0
4PUR4_CAEEL (Q19311) Probable phosphoribosylformylglycinamidine s... 29 6.8
5STON1_HUMAN (Q9Y6Q2) Stonin-1 (Stoned B-like factor) 28 8.9

>SPSE_BACSU (P39625) Spore coat polysaccharide biosynthesis protein spsE|
          Length = 373

 Score = 32.0 bits (71), Expect = 0.80
 Identities = 17/66 (25%), Positives = 28/66 (42%)
 Frame = +2

Query: 44  HCILYLPSQNWQKLGN*VPSQSSAVRLEITLLSMFPTGASCWNEKEEHPVDMPCLAKKTS 223
           HC+   P+          P + S + +   L + FP     +++  EHP + PC A +  
Sbjct: 180 HCVAKYPA----------PPEYSNLSVIPMLAAAFPEAVIGFSDHSEHPTEAPCAAVRLG 229

Query: 224 TKFCEK 241
            K  EK
Sbjct: 230 AKLIEK 235



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>FLGE_BORBU (Q44767) Flagellar hook protein flgE|
          Length = 442

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 2/73 (2%)
 Frame = +3

Query: 183 NIQSICLALQKKPQQSSVRRLI*SLNLDFKLLLAHEYCNRLTRGRRILV*QRSMLSSFGT 362
           +I+ + + +  K    S + +  + NLD +L L  E  N     R   V  +S+  SFG 
Sbjct: 154 DIEDLIIPIGDKEGAKSTKNVTFACNLDKRLPLIQEGANPADIARGTWVVNKSLYDSFGN 213

Query: 363 SSSIKCR--QDLN 395
            S ++ R  +DLN
Sbjct: 214 VSVLELRVVKDLN 226



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>IF2_LEGPL (Q5WT36) Translation initiation factor IF-2|
          Length = 868

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 18/41 (43%), Positives = 21/41 (51%)
 Frame = +2

Query: 125 EITLLSMFPTGASCWNEKEEHPVDMPCLAKKTSTKFCEKIN 247
           EIT++S      S   E EEHPV  P  A   + K  EKIN
Sbjct: 125 EITVVSEVSADTSELKETEEHPVIEPVAALDETVKEEEKIN 165



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>PUR4_CAEEL (Q19311) Probable phosphoribosylformylglycinamidine synthase (EC|
           6.3.5.3) (FGAM synthase) (FGAMS) (Formylglycinamide
           ribotide amidotransferase) (FGARAT) (Formylglycinamide
           ribotide synthetase)
          Length = 1343

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 22/79 (27%), Positives = 33/79 (41%)
 Frame = +2

Query: 59  LPSQNWQKLGN*VPSQSSAVRLEITLLSMFPTGASCWNEKEEHPVDMPCLAKKTSTKFCE 238
           L S NW+KL              +TLLS  P   S W E + HP            +  +
Sbjct: 50  LISSNWEKL--------------VTLLSHSPFETSVWKESQLHP------------EHGK 83

Query: 239 KINLKLKSGLQTPACS*IL 295
            I +  ++ ++T AC+ IL
Sbjct: 84  NIEIGPRTAVKTAACTNIL 102



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>STON1_HUMAN (Q9Y6Q2) Stonin-1 (Stoned B-like factor)|
          Length = 735

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 17/44 (38%), Positives = 22/44 (50%)
 Frame = +1

Query: 91  LSSFSIICSQARNNASFHVPNRRVMLE*KGGTSSRYALPCKKNL 222
           L S S+ C  A  NAS  VP+     + K G S    +P KKN+
Sbjct: 245 LRSLSMHCLCAEENASSFVPHTLFRSQPKSGWSFMLRIPEKKNM 288


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,732,943
Number of Sequences: 219361
Number of extensions: 1251548
Number of successful extensions: 3418
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 3350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3418
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3014947676
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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