ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl32a03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TRM1_ARATH (Q9LFU5) Probable N(2),N(2)-dimethylguanosine tRNA me... 52 7e-07
2TRM2_ARATH (Q9SRU7) Probable N(2),N(2)-dimethylguanosine tRNA me... 49 5e-06
3TRM1_HUMAN (Q9NXH9) N(2),N(2)-dimethylguanosine tRNA methyltrans... 33 0.45
4TRM1_MOUSE (Q3TX08) N(2),N(2)-dimethylguanosine tRNA methyltrans... 31 1.3
5RUSC2_HUMAN (Q8N2Y8) RUN and SH3 domain-containing protein 2 30 2.3
6RNH2_PARUW (Q6MDG7) Ribonuclease HII (EC 3.1.26.4) (RNase HII) 29 5.0
7RNH2_HAEDU (Q7VMF2) Ribonuclease HII (EC 3.1.26.4) (RNase HII) 29 5.0
8IFM3_HUMAN (Q01628) Interferon-induced transmembrane protein 3 (... 29 5.0
9ACF_PONPY (Q5R9H4) APOBEC1 complementation factor (APOBEC1-stimu... 29 5.0
10RAPSN_CHICK (O42393) 43 kDa receptor-associated protein of the s... 29 5.0
11POLG_JAEVN (P14403) Genome polyprotein [Contains: Capsid protein... 29 5.0
12RUSC2_MOUSE (Q80U22) RUN and SH3 domain-containing protein 2 29 5.0
13PCOC1_RAT (O08628) Procollagen C-endopeptidase enhancer 1 precur... 29 5.0
14ACF_HUMAN (Q9NQ94) APOBEC1 complementation factor (APOBEC1-stimu... 29 6.6
15DNAA_NOCFA (Q5Z3Z8) Chromosomal replication initiator protein dnaA 29 6.6
16DNMT1_CHICK (Q92072) DNA (cytosine-5)-methyltransferase 1 (EC 2.... 29 6.6
17CHH4_PENMO (O97386) Crustacean hyperglycemic hormones 4 precurso... 29 6.6
18IFM2_HUMAN (Q01629) Interferon-induced transmembrane protein 2 (... 28 8.6
19HR4_DROME (Q9W539) Hormone receptor 4 (dHR4) 28 8.6
20RAVR1_MOUSE (Q9CW46) Protein raver-1 28 8.6
21RNH2_ANADE (Q2IMM4) Ribonuclease HII (EC 3.1.26.4) (RNase HII) 28 8.6
22TFE2_HUMAN (P15923) Transcription factor E2-alpha (Immunoglobuli... 28 8.6

>TRM1_ARATH (Q9LFU5) Probable N(2),N(2)-dimethylguanosine tRNA|
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase)
          Length = 586

 Score = 52.0 bits (123), Expect = 7e-07
 Identities = 25/45 (55%), Positives = 31/45 (68%), Gaps = 3/45 (6%)
 Frame = -2

Query: 457 QEPQLQASFSQATEGLV---ARKSPRFLPNPEKHWGPKMKAGRPL 332
           +EP  QA FS+    L    A+K  RFLPNPEKHWGPK++AGR +
Sbjct: 482 KEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRTI 526



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>TRM2_ARATH (Q9SRU7) Probable N(2),N(2)-dimethylguanosine tRNA|
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase)
          Length = 599

 Score = 49.3 bits (116), Expect = 5e-06
 Identities = 23/45 (51%), Positives = 30/45 (66%), Gaps = 3/45 (6%)
 Frame = -2

Query: 457 QEPQLQASFSQATEGLV---ARKSPRFLPNPEKHWGPKMKAGRPL 332
           +EP  +  FS+    L    A+K  RFLPNPEKHWGPK++AGR +
Sbjct: 499 KEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQI 543



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>TRM1_HUMAN (Q9NXH9) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
           2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
           (tRNA 2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase)
          Length = 659

 Score = 32.7 bits (73), Expect = 0.45
 Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
 Frame = -2

Query: 454 EPQLQASFS---QATEGLVARKSPRFLPNPEKHWGPKMKA 344
           EP+LQA+F+    A      R   RF  NPE +WGP+ +A
Sbjct: 526 EPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRA 565



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>TRM1_MOUSE (Q3TX08) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
           2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
           (tRNA 2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase)
          Length = 663

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
 Frame = -2

Query: 454 EPQLQASFS---QATEGLVARKSPRFLPNPEKHWGPKMKA 344
           EP+L+A+F+    A      R   RF  NPE +WGP+ +A
Sbjct: 525 EPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRA 564



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>RUSC2_HUMAN (Q8N2Y8) RUN and SH3 domain-containing protein 2|
          Length = 1516

 Score = 30.4 bits (67), Expect = 2.3
 Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 5/50 (10%)
 Frame = +3

Query: 96  WFSQCKSAGNPFRSEKP-KADQALSVIGMDHI----PLPPTSSWASGSTR 230
           W   C SA  P  S++P K DQ +  +    +     LPP  SW SG +R
Sbjct: 817 WSHSCPSAVRPATSQQPQKEDQKILTLTEYRLHGTGSLPPLGSWRSGLSR 866



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>RNH2_PARUW (Q6MDG7) Ribonuclease HII (EC 3.1.26.4) (RNase HII)|
          Length = 222

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 12/44 (27%), Positives = 23/44 (52%)
 Frame = -2

Query: 157 WSAFGFSERKGFPADLHCENHSNLNVCSVNMTIFFAPLRARPEI 26
           W  +GF++ KG+  +LH E       C ++    F P+++  E+
Sbjct: 174 WPGYGFNQHKGYATELHLEALFKQGPCPIHRR-SFDPIKSMLEV 216



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>RNH2_HAEDU (Q7VMF2) Ribonuclease HII (EC 3.1.26.4) (RNase HII)|
          Length = 197

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 14/44 (31%), Positives = 22/44 (50%)
 Frame = -2

Query: 172 ITDNAWSAFGFSERKGFPADLHCENHSNLNVCSVNMTIFFAPLR 41
           I D  +  +GF++ KG+P  LH E  +       +    FAP+R
Sbjct: 150 ILDQQFPEYGFAQHKGYPTKLHIEKLAQFGATPFHRK-SFAPVR 192



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>IFM3_HUMAN (Q01628) Interferon-induced transmembrane protein 3|
           (Interferon-inducible protein 1-8U)
          Length = 133

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 19/46 (41%), Positives = 24/46 (52%)
 Frame = +3

Query: 72  TEQTFKLLWFSQCKSAGNPFRSEKPKADQALSVIGMDHIPLPPTSS 209
           T QTF    FS   S G P   E  K +  ++V+G  H P PPTS+
Sbjct: 4   TVQTF----FSPVNS-GQPPNYEMLKEEHEVAVLGAPHNPAPPTST 44



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>ACF_PONPY (Q5R9H4) APOBEC1 complementation factor (APOBEC1-stimulating|
           protein)
          Length = 587

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 23/89 (25%), Positives = 43/89 (48%), Gaps = 6/89 (6%)
 Frame = +3

Query: 132 RSEKPKADQALSVIGMDHIPLPPTSSWASGSTRWLRFTPWHETDRTGGASVGTAVKGNYS 311
           R +  +A +AL+   +D  P+  T +       ++R+T       TGG   GT ++G Y+
Sbjct: 276 REDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRG-----TGGR--GTMLQGEYT 328

Query: 312 LSMGSIFS------GLPAFIFGPQCFSGL 380
            S+G ++       G P F + PQ ++ +
Sbjct: 329 YSLGQVYDPTTTYLGAPVF-YAPQAYAAI 356



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>RAPSN_CHICK (O42393) 43 kDa receptor-associated protein of the synapse (RAPsyn)|
           (Acetylcholine receptor-associated 43 kDa protein) (43
           kDa postsynaptic protein)
          Length = 411

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = +3

Query: 273 GASVGTAVKGNYSLSMGSIFSGLPAFIFGPQCF 371
           G +V   + G  SLSMG+ F GL  F    +CF
Sbjct: 113 GTTVSLQLNGQVSLSMGNAFLGLSIFQKALECF 145



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>POLG_JAEVN (P14403) Genome polyprotein [Contains: Capsid protein C (Core|
            protein); Envelope protein M (Matrix protein); Major
            envelope protein E; Nonstructural protein 1 (NS1);
            Nonstructural protein 2A (NS2A); Flavivirin protease NS2B
            regulatory subunit;
          Length = 1440

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 27/103 (26%), Positives = 43/103 (41%), Gaps = 13/103 (12%)
 Frame = +3

Query: 45   RGAKKIVIFTEQTFKLLWFSQCKS-------------AGNPFRSEKPKADQALSVIGMDH 185
            R A K +  T++ F++ W +  KS                P   E P   +A + I ++ 
Sbjct: 820  RSAPKRLSMTQEKFEMGWKAWGKSILFAPELANSTFVVDGPETKECPDEHRAWNSIEIED 879

Query: 186  IPLPPTSSWASGSTRWLRFTPWHETDRTGGASVGTAVKGNYSL 314
                 TS+       WL+      TD   GA +GTAVKG+ ++
Sbjct: 880  FGFGITSTRV-----WLKIRE-ESTDECDGAIIGTAVKGHVAV 916



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>RUSC2_MOUSE (Q80U22) RUN and SH3 domain-containing protein 2|
          Length = 1514

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 17/50 (34%), Positives = 22/50 (44%), Gaps = 5/50 (10%)
 Frame = +3

Query: 96  WFSQCKSAGNPFRSEKP-KADQALSVIGMDHI----PLPPTSSWASGSTR 230
           W   C     P  S++P K DQ +  +    +     LPP  SW SG TR
Sbjct: 812 WSHTCPPTVRPATSQQPPKEDQKIPTLAEYRLHGTGSLPPLGSWRSGFTR 861



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>PCOC1_RAT (O08628) Procollagen C-endopeptidase enhancer 1 precursor|
           (Procollagen COOH-terminal proteinase enhancer 1)
           (Procollagen C-proteinase enhancer 1) (PCPE-1) (Type I
           procollagen COOH-terminal proteinase enhancer) (Type 1
           procollagen C-proteinase e
          Length = 468

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
 Frame = -2

Query: 430 SQATEGLVARKSPRFLPNPEKHWGPKMKAGRPLKILPIDK-L*FPFTAVPTLAPPVRSVS 254
           S  + G  A+  P+   +P+   GPK+K     K+ P++K    P T    +AP   S++
Sbjct: 281 SAPSPGEDAQHGPQSRSDPKTGTGPKVKPPSKPKVQPVEKPEGSPATQATPVAPDAPSIT 340

Query: 253 C 251
           C
Sbjct: 341 C 341



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>ACF_HUMAN (Q9NQ94) APOBEC1 complementation factor (APOBEC1-stimulating|
           protein)
          Length = 594

 Score = 28.9 bits (63), Expect = 6.6
 Identities = 23/89 (25%), Positives = 43/89 (48%), Gaps = 6/89 (6%)
 Frame = +3

Query: 132 RSEKPKADQALSVIGMDHIPLPPTSSWASGSTRWLRFTPWHETDRTGGASVGTAVKGNYS 311
           R +  +A +AL+   +D  P+  T +       ++R+T       TGG   GT ++G Y+
Sbjct: 276 REDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRG-----TGGR--GTMLQGEYT 328

Query: 312 LSMGSIFS------GLPAFIFGPQCFSGL 380
            S+G ++       G P F + PQ ++ +
Sbjct: 329 YSLGQVYDPTTTYLGAPVF-YAPQTYAAI 356



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>DNAA_NOCFA (Q5Z3Z8) Chromosomal replication initiator protein dnaA|
          Length = 662

 Score = 28.9 bits (63), Expect = 6.6
 Identities = 26/66 (39%), Positives = 35/66 (53%), Gaps = 4/66 (6%)
 Frame = +1

Query: 238 GSHHGMKP-IARGEQVWARL*KEITVCQWEVFLVVYRPSSL-DPSVFQD*AEILGSSV-- 405
           GS  G  P + R +Q W +L K ITV Q    L V  PSSL   ++ +D  E +  S+  
Sbjct: 22  GSADGSIPAVTRAQQAWLKLVKPITVAQGFALLSV--PSSLAQEAIERDLREPILRSLGR 79

Query: 406 RLNPQL 423
           RL PQ+
Sbjct: 80  RLGPQV 85



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>DNMT1_CHICK (Q92072) DNA (cytosine-5)-methyltransferase 1 (EC 2.1.1.37) (Dnmt1)|
            (DNA methyltransferase GgaI) (DNA MTase GgaI) (MCMT)
            (M.GgaI)
          Length = 1537

 Score = 28.9 bits (63), Expect = 6.6
 Identities = 24/66 (36%), Positives = 30/66 (45%), Gaps = 11/66 (16%)
 Frame = +3

Query: 159  ALSVIGMDHIPLPPTSSWA-----------SGSTRWLRFTPWHETDRTGGASVGTAVKGN 305
            AL    M HIPL P S W              STR LR+T  H   + G +S G A++G 
Sbjct: 1334 ALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTSTRKLRYT--HHEKKNGRSSSG-ALRGV 1390

Query: 306  YSLSMG 323
             S + G
Sbjct: 1391 CSCAEG 1396



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>CHH4_PENMO (O97386) Crustacean hyperglycemic hormones 4 precursor (Pm-SGP-IV)|
           [Contains: CHH precursor-related peptide 4 (CPRP 4);
           Crustacean hyperglycemic hormone 4 (CHH 4)]
          Length = 120

 Score = 28.9 bits (63), Expect = 6.6
 Identities = 21/62 (33%), Positives = 29/62 (46%)
 Frame = +3

Query: 201 TSSWASGSTRWLRFTPWHETDRTGGASVGTAVKGNYSLSMGSIFSGLPAFIFGPQCFSGL 380
           T S  S +   L  +P   + R+  AS  +A  GN+SLS  S+F      I+  Q    L
Sbjct: 6   TLSAVSAALLVLAASPSPASARSLDASPSSAFSGNHSLSKRSLFDPACTGIYDRQLLGKL 65

Query: 381 GR 386
           GR
Sbjct: 66  GR 67



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>IFM2_HUMAN (Q01629) Interferon-induced transmembrane protein 2|
           (Interferon-inducible protein 1-8D)
          Length = 132

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = +3

Query: 114 SAGNPFRSEKPKADQALSVIGMDHIPLPPTSS 209
           ++G P   E  K +Q ++++G  H P PPTS+
Sbjct: 12  NSGQPPNYEMLKEEQEVAMLGGPHNPAPPTST 43



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>HR4_DROME (Q9W539) Hormone receptor 4 (dHR4)|
          Length = 1518

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 16/48 (33%), Positives = 24/48 (50%)
 Frame = +1

Query: 115  QQETPSAQKNQKQTRHYQLLGWITYPFHQPLHGPLVQQGGYGSHHGMK 258
            QQ+  + Q++Q+Q +H Q         H PLH    Q  G+ SHH  +
Sbjct: 1032 QQQAAAQQQHQQQQQHQQHQQHQQQQLHSPLHHHHHQ--GHQSHHAQQ 1077



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>RAVR1_MOUSE (Q9CW46) Protein raver-1|
          Length = 748

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 17/44 (38%), Positives = 22/44 (50%)
 Frame = +1

Query: 127 PSAQKNQKQTRHYQLLGWITYPFHQPLHGPLVQQGGYGSHHGMK 258
           PS+    K+TR +   G    P   PL  P V  GG GS++G K
Sbjct: 523 PSSNLAGKETRGWGGSGRGRRPAEPPLPSPAVPGGGSGSNNGNK 566



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>RNH2_ANADE (Q2IMM4) Ribonuclease HII (EC 3.1.26.4) (RNase HII)|
          Length = 283

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 14/42 (33%), Positives = 21/42 (50%)
 Frame = -2

Query: 166 DNAWSAFGFSERKGFPADLHCENHSNLNVCSVNMTIFFAPLR 41
           D A   +GF+  KG+P   H +    L  C ++    FAP+R
Sbjct: 219 DLAHPGYGFARHKGYPTAEHFDALGRLGACPIHRR-SFAPVR 259



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>TFE2_HUMAN (P15923) Transcription factor E2-alpha (Immunoglobulin|
           enhancer-binding factor E12/E47) (Transcription factor
           3) (TCF-3) (Immunoglobulin transcription factor 1)
           (Transcription factor ITF-1) (Kappa-E2-binding factor)
          Length = 654

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 20/62 (32%), Positives = 24/62 (38%)
 Frame = +3

Query: 174 GMDHIPLPPTSSWASGSTRWLRFTPWHETDRTGGASVGTAVKGNYSLSMGSIFSGLPAFI 353
           G   +PLPP S     S     F   H+ +R G    G  V G   L   S FS  P   
Sbjct: 243 GSSPLPLPPGSGPVGSSGSSSTFGGLHQHERMGYQLHGAEVNG--GLPSASSFSSAPGAT 300

Query: 354 FG 359
           +G
Sbjct: 301 YG 302


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,605,337
Number of Sequences: 219361
Number of extensions: 1613933
Number of successful extensions: 4580
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 4440
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4575
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2909956200
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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