ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl31a12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1DPO3_STRP8 (Q8NZB5) DNA polymerase III polC-type (EC 2.7.7.7) (P... 32 0.47
2RGS6_CAEEL (Q18563) Regulator of G-protein signaling rgs-6 29 3.1
3DPO3_STRPY (P0C0B7) DNA polymerase III polC-type (EC 2.7.7.7) (P... 29 3.1
4DPO3_STRP6 (Q5X9U8) DNA polymerase III polC-type (EC 2.7.7.7) (P... 29 3.1
5DPO3_STRP3 (Q8K5S8) DNA polymerase III polC-type (EC 2.7.7.7) (P... 29 3.1
6DPO3_STRP1 (P0C0B8) DNA polymerase III polC-type (EC 2.7.7.7) (P... 29 3.1
7CDK7_MOUSE (Q03147) Cell division protein kinase 7 (EC 2.7.11.22... 29 3.1
8AP1M_DISOM (P47795) AP-1 complex subunit mu (Clathrin coat assem... 28 4.0
9KPYK_CLOAB (O08309) Pyruvate kinase (EC 2.7.1.40) (PK) 28 4.0
10GLT1_SCHPO (Q9C102) Putative glutamate synthase [NADPH] (EC 1.4.... 28 5.2
11GP109_RAT (Q80Z39) Nicotinic acid receptor (G-protein coupled re... 28 6.8
12GP109_MOUSE (Q9EP66) Nicotinic acid receptor (G-protein coupled ... 28 6.8
13INX10_CAEEL (Q22549) Innexin-10 28 6.8
14G109B_HUMAN (P49019) Nicotinic acid receptor 2 (G-protein couple... 27 8.9
15VL2_HPV31 (P17389) Minor capsid protein L2 27 8.9
16NUKM_BRAOL (P42027) NADH-ubiquinone oxidoreductase 20 kDa subuni... 27 8.9
17RPOA_SACOF (Q6ENT2) DNA-directed RNA polymerase alpha chain (EC ... 27 8.9
18RPOA_SACHY (Q6L370) DNA-directed RNA polymerase alpha chain (EC ... 27 8.9

>DPO3_STRP8 (Q8NZB5) DNA polymerase III polC-type (EC 2.7.7.7) (PolIII)|
          Length = 1465

 Score = 31.6 bits (70), Expect = 0.47
 Identities = 23/70 (32%), Positives = 32/70 (45%), Gaps = 8/70 (11%)
 Frame = +2

Query: 32  IKVTTGMQNQAVDWSSKRLKKNTRNCYQ--------FKYVFSTKQCTPGSTNLIHHN*RF 187
           IKVT  +Q   VD+S   L+   +  ++        FK  FS  + T     LI    RF
Sbjct: 72  IKVTFDIQAAQVDYSDDLLQAYYQEAFEHAPCNSASFKSSFSKLKVTYEDDKLIIAAPRF 131

Query: 188 VQNEHMFHNH 217
           V N+H  +NH
Sbjct: 132 VNNDHFRNNH 141



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>RGS6_CAEEL (Q18563) Regulator of G-protein signaling rgs-6|
          Length = 737

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 17/46 (36%), Positives = 21/46 (45%)
 Frame = +2

Query: 185 FVQNEHMFHNHNQTPPKSTCAHARHGNASFIFGLFIRPQHNEQTLD 322
           F+     F N   TP K  C  ARH   S   G  +RP  ++ TLD
Sbjct: 329 FLPANFSFKNPASTPTKLVCIRARH---SLSTGAVLRPLLHKYTLD 371



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>DPO3_STRPY (P0C0B7) DNA polymerase III polC-type (EC 2.7.7.7) (PolIII)|
          Length = 1465

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 8/70 (11%)
 Frame = +2

Query: 32  IKVTTGMQNQAVDWSSKRLKKNTRNCYQ--------FKYVFSTKQCTPGSTNLIHHN*RF 187
           IKVT  +Q   VD+S   L+   +  ++        FK  FS  + T     LI     F
Sbjct: 72  IKVTFDIQAAQVDYSDDLLQAYYQEAFEHAPCNSASFKSSFSKLKVTYEDDKLIIAAPGF 131

Query: 188 VQNEHMFHNH 217
           V N+H  +NH
Sbjct: 132 VNNDHFRNNH 141



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>DPO3_STRP6 (Q5X9U8) DNA polymerase III polC-type (EC 2.7.7.7) (PolIII)|
          Length = 1465

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 8/70 (11%)
 Frame = +2

Query: 32  IKVTTGMQNQAVDWSSKRLKKNTRNCYQ--------FKYVFSTKQCTPGSTNLIHHN*RF 187
           IKVT  +Q   VD+S   L+   +  ++        FK  FS  + T     LI     F
Sbjct: 72  IKVTFDIQAAQVDYSDDLLQAYYQEAFEHAPCNSASFKSSFSKLKVTYEDDKLIIAAPGF 131

Query: 188 VQNEHMFHNH 217
           V N+H  +NH
Sbjct: 132 VNNDHFRNNH 141



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>DPO3_STRP3 (Q8K5S8) DNA polymerase III polC-type (EC 2.7.7.7) (PolIII)|
          Length = 1465

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 8/70 (11%)
 Frame = +2

Query: 32  IKVTTGMQNQAVDWSSKRLKKNTRNCYQ--------FKYVFSTKQCTPGSTNLIHHN*RF 187
           IKVT  +Q   VD+S   L+   +  ++        FK  FS  + T     LI     F
Sbjct: 72  IKVTFDIQAAQVDYSDDLLQAYYQEAFEHAPCNSASFKSSFSKLKVTYEDDKLIIAAPGF 131

Query: 188 VQNEHMFHNH 217
           V N+H  +NH
Sbjct: 132 VNNDHFRNNH 141



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>DPO3_STRP1 (P0C0B8) DNA polymerase III polC-type (EC 2.7.7.7) (PolIII)|
          Length = 1465

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 8/70 (11%)
 Frame = +2

Query: 32  IKVTTGMQNQAVDWSSKRLKKNTRNCYQ--------FKYVFSTKQCTPGSTNLIHHN*RF 187
           IKVT  +Q   VD+S   L+   +  ++        FK  FS  + T     LI     F
Sbjct: 72  IKVTFDIQAAQVDYSDDLLQAYYQEAFEHAPCNSASFKSSFSKLKVTYEDDKLIIAAPGF 131

Query: 188 VQNEHMFHNH 217
           V N+H  +NH
Sbjct: 132 VNNDHFRNNH 141



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>CDK7_MOUSE (Q03147) Cell division protein kinase 7 (EC 2.7.11.22) (EC|
           2.7.11.23) (CDK-activating kinase) (CAK) (TFIIH basal
           transcription factor complex kinase subunit) (39 kDa
           protein kinase) (P39 Mo15) (Protein-tyrosine kinase
           MPK-7) (CR4 protein kinas
          Length = 346

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 15/36 (41%), Positives = 19/36 (52%), Gaps = 6/36 (16%)
 Frame = +3

Query: 180 EDLFKMNTCFITT------TKLPPSRPAPTPGMEMP 269
           + LF  N C  TT      TK   +RP PTPG ++P
Sbjct: 273 QGLFLFNPCTRTTASQALKTKYFSNRPGPTPGCQLP 308



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>AP1M_DISOM (P47795) AP-1 complex subunit mu (Clathrin coat assembly protein|
           AP47 homolog) (Clathrin coat-associated protein AP47
           homolog) (Golgi adaptor AP-1 47 kDa protein homolog)
           (HA1 47 kDa subunit homolog) (Clathrin assembly protein
           assembly protein
          Length = 418

 Score = 28.5 bits (62), Expect = 4.0
 Identities = 14/40 (35%), Positives = 21/40 (52%)
 Frame = -1

Query: 378 HTLFDLDGGMAVLLVPPWTSRVCSLCCGLMNKPKIKLAFP 259
           H+LF ++GG AV L   W S V    C  + + ++K   P
Sbjct: 3   HSLFLMNGGGAVFLEKHWRSVVSRSVCAYLLEAQLKAGQP 42



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>KPYK_CLOAB (O08309) Pyruvate kinase (EC 2.7.1.40) (PK)|
          Length = 473

 Score = 28.5 bits (62), Expect = 4.0
 Identities = 18/53 (33%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
 Frame = +1

Query: 31  NKGHYRYAKPGSRL-VEQKIKKKYSKLLPVQICF*HKAMYTRLYQFNPPQLKI 186
           N  H  +A+ G R+ + +K++ K +K  PV IC   K    R   FNP +L++
Sbjct: 34  NFSHGDHAEHGGRIALVKKVRAKLNK--PVAICLDTKGPEIRTGDFNPSKLEL 84



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>GLT1_SCHPO (Q9C102) Putative glutamate synthase [NADPH] (EC 1.4.1.13)|
            (NADPH-GOGAT)
          Length = 2111

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 19/51 (37%), Positives = 24/51 (47%), Gaps = 3/51 (5%)
 Frame = -1

Query: 369  FDLDGGMAVLL--VPPWTSRVCSLCCGLMNKPKIKL-AFPCLAWAQVDLGG 226
            FD D   A+ +  V PWT  V   C G M+   I + +   LA A   LGG
Sbjct: 920  FDFDSSQAIPIEQVEPWTEIVRRFCTGAMSYGSISMESHSSLAIAMNRLGG 970



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>GP109_RAT (Q80Z39) Nicotinic acid receptor (G-protein coupled receptor 109)|
           (G-protein coupled receptor HM74) (Protein PUMA-G)
          Length = 360

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = -1

Query: 147 VHCFVLKTYLNW*QFRVFFFNLLLDQSTAWFCIPVVTFIY 28
           + CF LK+   W   R+F FNL +       C+P +T  Y
Sbjct: 48  IFCFHLKS---WKSSRIFLFNLAVADFLLIICLPFLTDNY 84



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>GP109_MOUSE (Q9EP66) Nicotinic acid receptor (G-protein coupled receptor 109)|
           (G-protein coupled receptor HM74) (Protein PUMA-G)
          Length = 360

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = -1

Query: 147 VHCFVLKTYLNW*QFRVFFFNLLLDQSTAWFCIPVVTFIY 28
           + CF LK+   W   R+F FNL +       C+P +T  Y
Sbjct: 48  IFCFHLKS---WKSSRIFLFNLAVADFLLIICLPFLTDNY 84



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>INX10_CAEEL (Q22549) Innexin-10|
          Length = 559

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 15/56 (26%), Positives = 28/56 (50%)
 Frame = +2

Query: 89  KKNTRNCYQFKYVFSTKQCTPGSTNLIHHN*RFVQNEHMFHNHNQTPPKSTCAHAR 256
           K+N +N  Q  Y ++ +  TP      + N   +QN++ + N+ +TP  S    +R
Sbjct: 490 KQNQQNATQPPYCYTNQNPTP------YQNQNQIQNQNQYSNYYRTPSLSRGTDSR 539



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>G109B_HUMAN (P49019) Nicotinic acid receptor 2 (G-protein coupled receptor|
           109B) (G-protein coupled receptor HM74) (G-protein
           coupled receptor HM74B)
          Length = 387

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 14/40 (35%), Positives = 19/40 (47%)
 Frame = -1

Query: 147 VHCFVLKTYLNW*QFRVFFFNLLLDQSTAWFCIPVVTFIY 28
           + CF LK+   W   R+F FNL +       C+P V   Y
Sbjct: 51  IFCFHLKS---WKSSRIFLFNLAVADFLLIICLPFVMDYY 87



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>VL2_HPV31 (P17389) Minor capsid protein L2|
          Length = 466

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 16/60 (26%), Positives = 28/60 (46%)
 Frame = +3

Query: 111 TSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTKLPPSRPAPTPGMEMPALFSVYS 290
           TS ++ L+ S++       ST   +   M+T  ++T     +   P PG+  PA   +YS
Sbjct: 172 TSGHLLLSSSSI-------STHNYEEIPMDTFIVSTNNENITSSTPIPGVRRPARLGLYS 224



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>NUKM_BRAOL (P42027) NADH-ubiquinone oxidoreductase 20 kDa subunit,|
           mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3)
           (Complex I-20KD) (CI-20KD)
          Length = 215

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 20/62 (32%), Positives = 27/62 (43%)
 Frame = +3

Query: 99  LEIATSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTKLPPSRPAPTPGMEMPALF 278
           L +   S+   A S++H +LP  S  T       T    T   PPS  AP PG+   A F
Sbjct: 12  LPLVLQSHRAAAVSHLHTSLPALSPAT-------TPTSYTRPGPPSTSAPPPGLSKTAEF 64

Query: 279 SV 284
            +
Sbjct: 65  VI 66



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>RPOA_SACOF (Q6ENT2) DNA-directed RNA polymerase alpha chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase alpha subunit) (RNA
           polymerase alpha subunit)
          Length = 339

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 14/43 (32%), Positives = 25/43 (58%)
 Frame = +3

Query: 99  LEIATSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTKL 227
           LE+ + +   L ++N+H  L + S T EDL ++N+  +   KL
Sbjct: 277 LELPSRTYNCLKRANIHTLLDLLSKTEEDLMRINSFRMEDGKL 319



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>RPOA_SACHY (Q6L370) DNA-directed RNA polymerase alpha chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase alpha subunit) (RNA
           polymerase alpha subunit)
          Length = 339

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 14/43 (32%), Positives = 25/43 (58%)
 Frame = +3

Query: 99  LEIATSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTKL 227
           LE+ + +   L ++N+H  L + S T EDL ++N+  +   KL
Sbjct: 277 LELPSRTYNCLKRANIHTLLDLLSKTEEDLMRINSFRMEDGKL 319


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,397,540
Number of Sequences: 219361
Number of extensions: 1352299
Number of successful extensions: 3631
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 3507
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3627
length of database: 80,573,946
effective HSP length: 109
effective length of database: 56,663,597
effective search space used: 1359926328
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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