ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl30a02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1YLB5_CAEEL (P46580) Hypothetical protein C34E10.5 in chromosome III 31 2.0
2GLSK_HUMAN (O94925) Glutaminase kidney isoform, mitochondrial pr... 31 2.0
3GLSK_RAT (P13264) Glutaminase kidney isoform, mitochondrial prec... 30 3.4
4TILS_MANSM (Q65UE9) tRNA(Ile)-lysidine synthase (EC 6.3.4.-) (tR... 24 7.5
5IRK14_RAT (O70596) ATP-sensitive inward rectifier potassium chan... 29 7.6
6IRK14_MOUSE (Q8JZN3) ATP-sensitive inward rectifier potassium ch... 29 7.6
7VD05_VARV (P33069) Protein D5 28 9.9

>YLB5_CAEEL (P46580) Hypothetical protein C34E10.5 in chromosome III|
          Length = 734

 Score = 30.8 bits (68), Expect = 2.0
 Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
 Frame = -1

Query: 344 SYVRPLPSCALEIETGMPLVTSSCPILTAFLPLHGH-TPEYKSDHMKCVKHFTQRHLRPN 168
           SYV+P+ S  +       +   S P L+  +P HG   PE   D M  ++ + Q H+R N
Sbjct: 534 SYVKPIMSTHIH----QTIKAQSIPYLSRAIPSHGRGEPELDEDEM-WIQKYPQGHVRNN 588

Query: 167 TD 162
            D
Sbjct: 589 MD 590



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>GLSK_HUMAN (O94925) Glutaminase kidney isoform, mitochondrial precursor (EC|
           3.5.1.2) (GLS) (L-glutamine amidohydrolase)
           (K-glutaminase)
          Length = 669

 Score = 30.8 bits (68), Expect = 2.0
 Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 5/47 (10%)
 Frame = -3

Query: 396 WEKAKKGGGDKLIMLPPQLRSSTPFL-----CAGDRNRHAPGDLVMP 271
           +E AKK  G K+    PQL   +P L     C  D  RH+ GD  +P
Sbjct: 235 YESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVP 281



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>GLSK_RAT (P13264) Glutaminase kidney isoform, mitochondrial precursor (EC|
           3.5.1.2) (GLS) (L-glutamine amidohydrolase)
           (K-glutaminase) [Contains: Glutaminase kidney isoform 68
           kDa chain; Glutaminase kidney isoform 65 kDa chain]
          Length = 674

 Score = 30.0 bits (66), Expect = 3.4
 Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 5/47 (10%)
 Frame = -3

Query: 396 WEKAKKGGGDKLIMLPPQLRSSTPFL-----CAGDRNRHAPGDLVMP 271
           +E AKK  G K+    PQL   +P L     C  D  RH+ GD  +P
Sbjct: 240 YESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSIGDTKVP 286



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>TILS_MANSM (Q65UE9) tRNA(Ile)-lysidine synthase (EC 6.3.4.-)|
           (tRNA(Ile)-lysidine synthetase)
           (tRNA(Ile)-2-lysyl-cytidine synthase)
          Length = 426

 Score = 24.3 bits (51), Expect(2) = 7.5
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = -3

Query: 354 LPPQLRSSTPFLCAGDRNRHAPG 286
           +PP LR+ TP +  GD+ + A G
Sbjct: 397 VPPWLRNRTPLIFYGDQLKSAVG 419



 Score = 23.1 bits (48), Expect(2) = 7.5
 Identities = 8/19 (42%), Positives = 12/19 (63%)
 Frame = -1

Query: 470 REV*KVWQGIGFPPRTRKR 414
           +++ KVWQ +  PP  R R
Sbjct: 386 QDIKKVWQNLNVPPWLRNR 404



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>IRK14_RAT (O70596) ATP-sensitive inward rectifier potassium channel 14|
           (Potassium channel, inwardly rectifying subfamily J
           member 14) (Inward rectifier K(+) channel Kir2.4) (IRK4)
          Length = 434

 Score = 28.9 bits (63), Expect = 7.6
 Identities = 13/24 (54%), Positives = 17/24 (70%)
 Frame = +2

Query: 371 PPPFFAFSQLPTFKSAFLFLEESQ 442
           PPP   FSQ+ +F +AFLF  E+Q
Sbjct: 120 PPPAPCFSQVASFLAAFLFALETQ 143



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>IRK14_MOUSE (Q8JZN3) ATP-sensitive inward rectifier potassium channel 14|
           (Potassium channel, inwardly rectifying subfamily J
           member 14) (Inward rectifier K(+) channel Kir2.4) (IRK4)
          Length = 434

 Score = 28.9 bits (63), Expect = 7.6
 Identities = 13/24 (54%), Positives = 17/24 (70%)
 Frame = +2

Query: 371 PPPFFAFSQLPTFKSAFLFLEESQ 442
           PPP   FSQ+ +F +AFLF  E+Q
Sbjct: 120 PPPAPCFSQVASFLAAFLFALETQ 143



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>VD05_VARV (P33069) Protein D5|
          Length = 785

 Score = 28.5 bits (62), Expect = 9.9
 Identities = 20/73 (27%), Positives = 36/73 (49%), Gaps = 4/73 (5%)
 Frame = +2

Query: 44  NLEENNNKIASCAKLDYYYYCSTLEQLYS*VQ*T*HLHPHQYLVEDDAV*SVSRISYDHS 223
           +L+ENN        +DY   C+  ++         H HPHQ  +E+DA+  + +    HS
Sbjct: 263 DLDENNFTTVPLV-IDYVTPCALCKKRS-------HKHPHQLSLENDAI-RIYKTGNPHS 313

Query: 224 C----IPVCGHEV 250
           C    +P+ G+++
Sbjct: 314 CKVKIVPLDGNKL 326


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,289,465
Number of Sequences: 219361
Number of extensions: 1454100
Number of successful extensions: 3321
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 3256
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3321
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3362826254
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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