ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl29h10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GLSK_HUMAN (O94925) Glutaminase kidney isoform, mitochondrial pr... 32 0.52
2GLSK_RAT (P13264) Glutaminase kidney isoform, mitochondrial prec... 32 0.89
3YLB5_CAEEL (P46580) Hypothetical protein C34E10.5 in chromosome III 31 1.5
4AROE_THETN (Q8RAG2) Shikimate dehydrogenase (EC 1.1.1.25) 29 5.8
5VD05_VARV (P33069) Protein D5 28 7.6

>GLSK_HUMAN (O94925) Glutaminase kidney isoform, mitochondrial precursor (EC|
           3.5.1.2) (GLS) (L-glutamine amidohydrolase)
           (K-glutaminase)
          Length = 669

 Score = 32.3 bits (72), Expect = 0.52
 Identities = 18/47 (38%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
 Frame = -3

Query: 396 WEKAKKEXGDKLIMLPPQLRSSTPFL-----CAGDRNRHAPGDLVMP 271
           +E AKK+ G K+    PQL   +P L     C  D  RH+ GD  +P
Sbjct: 235 YESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVP 281



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>GLSK_RAT (P13264) Glutaminase kidney isoform, mitochondrial precursor (EC|
           3.5.1.2) (GLS) (L-glutamine amidohydrolase)
           (K-glutaminase) [Contains: Glutaminase kidney isoform 68
           kDa chain; Glutaminase kidney isoform 65 kDa chain]
          Length = 674

 Score = 31.6 bits (70), Expect = 0.89
 Identities = 18/47 (38%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
 Frame = -3

Query: 396 WEKAKKEXGDKLIMLPPQLRSSTPFL-----CAGDRNRHAPGDLVMP 271
           +E AKK+ G K+    PQL   +P L     C  D  RH+ GD  +P
Sbjct: 240 YESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSIGDTKVP 286



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>YLB5_CAEEL (P46580) Hypothetical protein C34E10.5 in chromosome III|
          Length = 734

 Score = 30.8 bits (68), Expect = 1.5
 Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
 Frame = -1

Query: 344 SYVRPLPSCALEIETGMPLVTSSCPILTAFLPLHGH-TPEYKSDHMKCVKHFTQRHLRPN 168
           SYV+P+ S  +       +   S P L+  +P HG   PE   D M  ++ + Q H+R N
Sbjct: 534 SYVKPIMSTHIH----QTIKAQSIPYLSRAIPSHGRGEPELDEDEM-WIQKYPQGHVRNN 588

Query: 167 TD 162
            D
Sbjct: 589 MD 590



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>AROE_THETN (Q8RAG2) Shikimate dehydrogenase (EC 1.1.1.25)|
          Length = 280

 Score = 28.9 bits (63), Expect = 5.8
 Identities = 15/55 (27%), Positives = 26/55 (47%)
 Frame = +2

Query: 230 PVCGHEVAKRRLKWGMTRSPGACLFLSPAHRKGVDERNCGGNMINLSPXSFFAFS 394
           PV    VAK    + +  +P   LFL  A + GV   N    ++N +  +F+ ++
Sbjct: 209 PVSEEVVAKANFVYDLIYNPSETLFLKYARKNGVKSANGLSMLVNQASYAFYLWT 263



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>VD05_VARV (P33069) Protein D5|
          Length = 785

 Score = 28.5 bits (62), Expect = 7.6
 Identities = 20/73 (27%), Positives = 36/73 (49%), Gaps = 4/73 (5%)
 Frame = +2

Query: 44  NLEENNNKIASCAKLDYYYYCSTLEQLYS*VQ*T*HLHPHQYLVEDDAV*SVSRISYDHS 223
           +L+ENN        +DY   C+  ++         H HPHQ  +E+DA+  + +    HS
Sbjct: 263 DLDENNFTTVPLV-IDYVTPCALCKKRS-------HKHPHQLSLENDAI-RIYKTGNPHS 313

Query: 224 C----IPVCGHEV 250
           C    +P+ G+++
Sbjct: 314 CKVKIVPLDGNKL 326


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,480,631
Number of Sequences: 219361
Number of extensions: 1186550
Number of successful extensions: 2520
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2520
length of database: 80,573,946
effective HSP length: 101
effective length of database: 58,418,485
effective search space used: 2570413340
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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