ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl28h01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AMPN_ECOLI (P04825) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoa... 74 8e-14
2AMPN_CAUCR (P37893) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoa... 72 3e-13
3AMPN_HAEIN (P45274) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoa... 65 5e-11
4AMP1_PLAFQ (O96935) M1-family aminopeptidase (EC 3.4.11.-) (Pfa-M1) 54 1e-07
5KEX1_YEAST (P09620) Carboxypeptidase KEX1 precursor (EC 3.4.16.6... 30 1.8
6RPOM_HUMAN (O00411) DNA-directed RNA polymerase, mitochondrial p... 29 3.0
7SF3B5_DROME (Q9VHI4) Probable splicing factor 3B subunit 5 (SF3b... 28 5.2
8RPE65_CYNPY (Q8AXN9) Retinal pigment epithelium-specific 65 kDa ... 28 5.2
9TILS_PROMA (Q7V9L9) tRNA(Ile)-lysidine synthase (EC 6.3.4.-) (tR... 28 5.2
10SYY_HELPY (P56417) Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Tyrosin... 28 6.8
11HEM1_HALSA (Q9HP72) Glutamyl-tRNA reductase (EC 1.2.1.70) (GluTR) 27 8.9
12SF3BB_ARATH (P58728) Protein At4g14342 27 8.9
13SF3BA_ARATH (Q9LW64) Protein At3g23325 27 8.9
14FAK1_XENLA (Q91738) Focal adhesion kinase 1 (EC 2.7.10.2) (FADK ... 27 8.9
15E2F5_HUMAN (Q15329) Transcription factor E2F5 (E2F-5) 27 8.9
16OPDA_HAEIN (P44573) Oligopeptidase A (EC 3.4.24.70) 27 8.9
17GLB1_CHLEU (Q08753) Globin LI637 27 8.9
18SYY_HELPJ (Q9ZL69) Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Tyrosin... 27 8.9

>AMPN_ECOLI (P04825) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide|
            hydrolase)
          Length = 869

 Score = 73.9 bits (180), Expect = 8e-14
 Identities = 39/82 (47%), Positives = 53/82 (64%), Gaps = 1/82 (1%)
 Frame = -2

Query: 404  FCGS-PVSFHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRWRRYDETRQALAKAQL 228
            F GS P +FHA+DGSGY FL E++  L+  NPQVASR++    R +RYD  RQ   +A L
Sbjct: 788  FAGSNPAAFHAEDGSGYLFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAAL 847

Query: 227  EMIISANGLSENVYEIASKSLA 162
            E +     LS ++YE  +K+LA
Sbjct: 848  EQLKGLENLSGDLYEKITKALA 869



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>AMPN_CAUCR (P37893) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide|
            hydrolase)
          Length = 863

 Score = 72.0 bits (175), Expect = 3e-13
 Identities = 34/77 (44%), Positives = 48/77 (62%)
 Frame = -2

Query: 395  SPVSFHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRWRRYDETRQALAKAQLEMII 216
            +P  FH   G+GY FL + +L++D  NP  A+R+V     WRRY      L +AQLE I+
Sbjct: 786  NPARFHDPSGAGYAFLADEILKVDAFNPMTAARLVEPLGGWRRYKPELGDLMRAQLERIV 845

Query: 215  SANGLSENVYEIASKSL 165
            +   LS+NV E+ASK+L
Sbjct: 846  AHPNLSKNVLELASKAL 862



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>AMPN_HAEIN (P45274) Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide|
            hydrolase)
          Length = 869

 Score = 64.7 bits (156), Expect = 5e-11
 Identities = 30/74 (40%), Positives = 49/74 (66%)
 Frame = -2

Query: 386  SFHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRWRRYDETRQALAKAQLEMIISAN 207
            +FH   GSGY+FL +V+++L++ NPQVA+R++    R+ R+D  RQ L K  LE +    
Sbjct: 795  AFHNVSGSGYRFLTDVLIRLNESNPQVAARLIEPLIRFSRFDAQRQTLMKRALERLSVVE 854

Query: 206  GLSENVYEIASKSL 165
             LS++++E   K+L
Sbjct: 855  NLSKDLFEKIEKAL 868



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>AMP1_PLAFQ (O96935) M1-family aminopeptidase (EC 3.4.11.-) (Pfa-M1)|
          Length = 1085

 Score = 53.5 bits (127), Expect = 1e-07
 Identities = 23/67 (34%), Positives = 36/67 (53%)
 Frame = -2

Query: 383  FHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRWRRYDETRQALAKAQLEMIISANG 204
            FH   G GYK + EV+ + DK NP VA+++   F  W + D  RQ L   ++  ++    
Sbjct: 1010 FHDISGKGYKLIAEVITKTDKFNPMVATQLCEPFKLWNKLDTKRQELMLNEMNTMLQEPN 1069

Query: 203  LSENVYE 183
            +S N+ E
Sbjct: 1070 ISNNLKE 1076



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>KEX1_YEAST (P09620) Carboxypeptidase KEX1 precursor (EC 3.4.16.6)|
           (Carboxypeptidase D)
          Length = 729

 Score = 29.6 bits (65), Expect = 1.8
 Identities = 19/57 (33%), Positives = 28/57 (49%), Gaps = 9/57 (15%)
 Frame = +2

Query: 50  HAKYSKIYSVKYTFKALVMVKG----STGKIKFVSLHQETKL-----PNFSRLSHKH 193
           H K+SKI    Y  KAL++  G    +T  + ++    E KL     PNF  L++ H
Sbjct: 214 HNKFSKIDGDTYDLKALLIGNGWIDPNTQSLSYLPFAMEKKLIDESNPNFKHLTNAH 270



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>RPOM_HUMAN (O00411) DNA-directed RNA polymerase, mitochondrial precursor (EC|
            2.7.7.6) (MtRPOL)
          Length = 1230

 Score = 28.9 bits (63), Expect = 3.0
 Identities = 17/57 (29%), Positives = 25/57 (43%)
 Frame = +2

Query: 26   TIVACYFTHAKYSKIYSVKYTFKALVMVKGSTGKIKFVSLHQETKLPNFSRLSHKHF 196
            T + CY     +  ++   +T  A V V     + +FV LH E  L + SR   K F
Sbjct: 1135 TALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRF 1191



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>SF3B5_DROME (Q9VHI4) Probable splicing factor 3B subunit 5 (SF3b5)|
           (Pre-mRNA-splicing factor SF3b 10 kDa subunit)
          Length = 85

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 11/15 (73%), Positives = 12/15 (80%)
 Frame = +2

Query: 275 IERMLTPCGMPPEDL 319
           +ERML PCG PPE L
Sbjct: 69  MERMLQPCGPPPEKL 83



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>RPE65_CYNPY (Q8AXN9) Retinal pigment epithelium-specific 65 kDa protein|
          Length = 532

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 17/56 (30%), Positives = 24/56 (42%)
 Frame = -1

Query: 234 PVGDDYFSQWTL*KCL*DSLEKFGSLVS*CKETNLILPVDPFTITSALKVYLTEYI 67
           PVG+DY++                     C ETN I  V+P T+ +  KV L  Y+
Sbjct: 137 PVGEDYYA---------------------CTETNYITKVNPETLETIKKVDLCNYV 171



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>TILS_PROMA (Q7V9L9) tRNA(Ile)-lysidine synthase (EC 6.3.4.-)|
           (tRNA(Ile)-lysidine synthetase)
           (tRNA(Ile)-2-lysyl-cytidine synthase)
          Length = 336

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 16/66 (24%), Positives = 32/66 (48%)
 Frame = -2

Query: 356 KFLGEVVLQLDKINPQVASRMVSAFSRWRRYDETRQALAKAQLEMIISANGLSENVYEIA 177
           K   E++  L+ ++PQ   R+ +   R     + +  LA   L  ++++ GLS +     
Sbjct: 216 KIRAEIIPVLESLHPQSTIRISNLAERLTSLQKDQHQLAHLALGALLTSTGLSRSKMTKL 275

Query: 176 SKSLAA 159
           SK++ A
Sbjct: 276 SKTVRA 281



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>SYY_HELPY (P56417) Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Tyrosine--tRNA|
           ligase) (TyrRS)
          Length = 402

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 23/69 (33%), Positives = 31/69 (44%)
 Frame = -2

Query: 320 INPQVASRMVSAFSRWRRYDETRQALAKAQLEMIISANGLSENVYEIASKSLAA*FPDAK 141
           +   +AS +V+     R YD  +   AK Q   + SAN L E + E +         D  
Sbjct: 294 VKEDLASEIVA-----RYYDNDQAIKAKEQFSKVFSANLLPEILSE-SDFDEGVGILDVL 347

Query: 140 KQI*FCQST 114
           KQI FC ST
Sbjct: 348 KQIGFCPST 356



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>HEM1_HALSA (Q9HP72) Glutamyl-tRNA reductase (EC 1.2.1.70) (GluTR)|
          Length = 436

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 3/65 (4%)
 Frame = +1

Query: 193 FSESPLAEIIISNWALARACLVSSYLLHRENADTMRDAT*GFILSSCKTT---SPKNL*P 363
           F+ + +AEI+I+N   ARA  V+  +     A T+ DA      +    T   SP+++ P
Sbjct: 196 FASATVAEIVIANRTPARAVRVADMVSVPAEATTLADARGRLDAADVVVTATGSPEHVLP 255

Query: 364 EPSFA 378
             +FA
Sbjct: 256 ASAFA 260



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>SF3BB_ARATH (P58728) Protein At4g14342|
          Length = 87

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 9/23 (39%), Positives = 15/23 (65%)
 Frame = +2

Query: 245 ELVSSRHISSIERMLTPCGMPPE 313
           E +     + +++ML PCG+PPE
Sbjct: 61  ESIGRERYNFMQKMLLPCGLPPE 83



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>SF3BA_ARATH (Q9LW64) Protein At3g23325|
          Length = 87

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 9/23 (39%), Positives = 15/23 (65%)
 Frame = +2

Query: 245 ELVSSRHISSIERMLTPCGMPPE 313
           E +     + +++ML PCG+PPE
Sbjct: 61  ESIGRERYNFMQKMLLPCGLPPE 83



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>FAK1_XENLA (Q91738) Focal adhesion kinase 1 (EC 2.7.10.2) (FADK 1) (pp125FAK)|
          Length = 1068

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
 Frame = +2

Query: 131 KFVSLHQETKLPNFS-RLSHKHFQRVHWL 214
           K V  H+   + ++  RLSH H + VHWL
Sbjct: 70  KIVDSHKVKNVASYGLRLSHLHSEEVHWL 98



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>E2F5_HUMAN (Q15329) Transcription factor E2F5 (E2F-5)|
          Length = 346

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 21/85 (24%), Positives = 40/85 (47%)
 Frame = +2

Query: 152 ETKLPNFSRLSHKHFQRVHWLK*SSPTGP*QELVSSRHISSIERMLTPCGMPPEDLFCRA 331
           E  +P   +   K +Q    +   S +GP   L+ ++  SS + ++ P   PP+DL   +
Sbjct: 199 EVPIPEMGQNGQKKYQ----INLKSHSGPIHVLLINKESSSSKPVVFPVP-PPDDLTQPS 253

Query: 332 VKQLHPRTCNQNHLSRENSQVTHKS 406
            + L P T  ++ ++ +N    H S
Sbjct: 254 SQSLTPVTPQKSSMATQNLPEQHVS 278



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>OPDA_HAEIN (P44573) Oligopeptidase A (EC 3.4.24.70)|
          Length = 681

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 16/60 (26%), Positives = 28/60 (46%)
 Frame = -2

Query: 347 GEVVLQLDKINPQVASRMVSAFSRWRRYDETRQALAKAQLEMIISANGLSENVYEIASKS 168
           GE+V +L ++N Q ++ ++ A   W +  E    LA           GL E+  + A +S
Sbjct: 154 GEIVARLSELNSQFSNNVLDATMGWEKLIENEAELA-----------GLPESALQAAQQS 202



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>GLB1_CHLEU (Q08753) Globin LI637|
          Length = 164

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 26/96 (27%), Positives = 40/96 (41%)
 Frame = +2

Query: 32  VACYFTHAKYSKIYSVKYTFKALVMVKGSTGKIKFVSLHQETKLPNFSRLSHKHFQRVHW 211
           V+ YF++       S ++ F A  +   S  K K +    +  +P+   LS  HFQ V  
Sbjct: 72  VSTYFSNTDMKVQRSKQFAFLAYALGGASEWKGKDMRTAHKDLVPH---LSDVHFQAV-- 126

Query: 212 LK*SSPTGP*QELVSSRHISSIERMLTPCGMPPEDL 319
                          +RH+S     LT  G+PPED+
Sbjct: 127 ---------------ARHLSDT---LTELGVPPEDI 144



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>SYY_HELPJ (Q9ZL69) Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Tyrosine--tRNA|
           ligase) (TyrRS)
          Length = 402

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 23/69 (33%), Positives = 31/69 (44%)
 Frame = -2

Query: 320 INPQVASRMVSAFSRWRRYDETRQALAKAQLEMIISANGLSENVYEIASKSLAA*FPDAK 141
           +   +AS +V+     R YD  +   AK Q   + SAN L E + E +         D  
Sbjct: 294 VKEDLASEIVA-----RYYDNDQAFKAKEQFSKVFSANLLPEILLE-SDFDEGVGVLDVL 347

Query: 140 KQI*FCQST 114
           KQI FC ST
Sbjct: 348 KQIGFCPST 356


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,898,852
Number of Sequences: 219361
Number of extensions: 1135234
Number of successful extensions: 2538
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 2495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2538
length of database: 80,573,946
effective HSP length: 111
effective length of database: 56,224,875
effective search space used: 1349397000
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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