ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl28e12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (E... 88 5e-18
2EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (E... 87 8e-18
3PO2F1_CHICK (P15143) POU domain, class 2, transcription factor 1... 30 1.5
4GSPN_PECCC (P31710) General secretion pathway protein N (Pectic ... 29 2.5
5PO2F1_RAT (P31503) POU domain, class 2, transcription factor 1 (... 29 3.3
6PO2F1_PIG (Q29076) POU domain, class 2, transcription factor 1 (... 29 3.3
7SLAF1_CANFA (Q95MM9) Signaling lymphocytic activation molecule p... 28 5.6
8PCX_DROME (P18490) Protein pecanex 28 5.6
9CT174_HUMAN (Q5JPB2) Protein C20orf174 28 7.3
10RPOB_FUSNN (Q8RHI6) DNA-directed RNA polymerase beta chain (EC 2... 28 7.3
11NU5M_ELEMA (Q2I3G4) NADH-ubiquinone oxidoreductase chain 5 (EC 1... 28 7.3
12ABCB7_HUMAN (O75027) ATP-binding cassette sub-family B member 7,... 28 7.3
13LEG2_PIG (Q29373) Galectin-2 (Fragment) 28 7.3
14ABCB7_RAT (Q704E8) ATP-binding cassette sub-family B member 7, m... 27 9.5

>EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
            (Endo-beta-mannosidase) (AtEBM) [Contains:
            Mannosylglycoprotein endo-beta-mannosidase 31 kDa
            subunit; Mannosylglycoprotein endo-beta-mannosidase 28
            kDa subunit; Mannosylglycopro
          Length = 943

 Score = 88.2 bits (217), Expect = 5e-18
 Identities = 45/68 (66%), Positives = 51/68 (75%), Gaps = 2/68 (2%)
 Frame = -2

Query: 354  VAFFLHFSVHTSESSIGENYRDTRILPVHYSDNYFSLTPGEKMTTDISFEAPEGSK--PR 181
            VAFFL FSVH +E+      +DTRILPVHYSDNYFSL PGE M+  ISF AP G K  PR
Sbjct: 872  VAFFLRFSVHNAETE----KQDTRILPVHYSDNYFSLVPGESMSFKISFAAPTGMKKSPR 927

Query: 180  VILRGWNY 157
            V+L+GWNY
Sbjct: 928  VMLQGWNY 935



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>EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
            (Endo-beta-mannosidase) [Contains: Mannosylglycoprotein
            endo-beta-mannosidase 31 kDa subunit;
            Mannosylglycoprotein endo-beta-mannosidase 28 kDa
            subunit; Mannosylglycoprotein end
          Length = 952

 Score = 87.4 bits (215), Expect = 8e-18
 Identities = 44/70 (62%), Positives = 45/70 (64%)
 Frame = -2

Query: 354  VAFFLHFSVHTSESSIGENYRDTRILPVHYSDNYFSLTPGEKMTTDISFEAPEGSKPRVI 175
            VAFFLHFSVH  +    E   D RILPVHYSDNYFSL PGE     ISFE P G  PRV 
Sbjct: 880  VAFFLHFSVHAVKKDENE-IEDLRILPVHYSDNYFSLVPGETTNISISFEVPHGVTPRVS 938

Query: 174  LRGWNYHLNH 145
            LRGWN    H
Sbjct: 939  LRGWNCSEEH 948



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>PO2F1_CHICK (P15143) POU domain, class 2, transcription factor 1|
           (Octamer-binding transcription factor 1) (Oct-1) (OTF-1)
           (NF-A1)
          Length = 739

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 20/58 (34%), Positives = 27/58 (46%)
 Frame = -3

Query: 299 ITGTLESFRYTTQTTIFRSPPGRR*QLTSPSRHPKDPSPGSF*EAGTII*TMLSQCES 126
           +TGT E+    T T I  +PP     +TSPS  P   +  S  EA +   T  +Q  S
Sbjct: 490 VTGTTETTSNNTATVISTAPPASS-AVTSPSLSPSPSASASISEASSASETSTTQTTS 546



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>GSPN_PECCC (P31710) General secretion pathway protein N (Pectic enzymes|
           secretion protein OUTN)
          Length = 248

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 15/46 (32%), Positives = 24/46 (52%)
 Frame = -2

Query: 300 NYRDTRILPVHYSDNYFSLTPGEKMTTDISFEAPEGSKPRVILRGW 163
           ++R   +  VH++  +    P      DI+F+ PEG   R I+RGW
Sbjct: 57  SWRTLTLDDVHWNITFSDFMPA----LDIAFKNPEGIAGRGIIRGW 98



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>PO2F1_RAT (P31503) POU domain, class 2, transcription factor 1|
           (Octamer-binding transcription factor 1) (Oct-1) (OTF-1)
           (NF-A1) (Fragment)
          Length = 632

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 20/58 (34%), Positives = 27/58 (46%)
 Frame = -3

Query: 299 ITGTLESFRYTTQTTIFRSPPGRR*QLTSPSRHPKDPSPGSF*EAGTII*TMLSQCES 126
           +TGT +S    T T I  +PP     +TSPS  P   +  S  EA +   T  +Q  S
Sbjct: 383 LTGTTDSTSNNTATVISTAPPASS-AVTSPSLSPSPSASASTSEASSASETSTTQTTS 439



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>PO2F1_PIG (Q29076) POU domain, class 2, transcription factor 1|
           (Octamer-binding transcription factor 1) (Oct-1) (OTF-1)
           (NF-A1)
          Length = 745

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 19/58 (32%), Positives = 27/58 (46%)
 Frame = -3

Query: 299 ITGTLESFRYTTQTTIFRSPPGRR*QLTSPSRHPKDPSPGSF*EAGTII*TMLSQCES 126
           +TGT ++    T T I  +PP     +TSPS  P   + GS  E  +   T  +Q  S
Sbjct: 496 VTGTTDTTSNNTATVISTAPPASS-AVTSPSLSPSPSASGSTSEVSSASETSTTQTTS 552



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>SLAF1_CANFA (Q95MM9) Signaling lymphocytic activation molecule precursor (CD150|
           antigen homolog)
          Length = 342

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
 Frame = -2

Query: 243 TPGEKMTTDI-SFEAPEGSKPRVILRGWNYHLNH 145
           +PG  +   I S + PEG  PR +  G+ +HL +
Sbjct: 69  SPGNSIKKKIVSLDLPEGGSPRYLENGYKFHLEN 102



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>PCX_DROME (P18490) Protein pecanex|
          Length = 3433

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = +1

Query: 28  LLVYKIAKPFMSALYWGCTYFTWILF 105
           L +Y +  PF++ LY+  T+ TW L+
Sbjct: 38  LWLYLLCSPFVAYLYFPSTWLTWCLY 63



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>CT174_HUMAN (Q5JPB2) Protein C20orf174|
          Length = 1082

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 16/39 (41%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
 Frame = +1

Query: 181  PGLGSFGCLEGDVSCHLL-PGGERKIVV*VVYRKDSSVP 294
            P  G +GC E  V C  L   G ++ V  ++ RKDS VP
Sbjct: 996  PSRGQYGCGEMTVPCPSLGSDGRKRQVSGLITRKDSVVP 1034



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>RPOB_FUSNN (Q8RHI6) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP|
           beta subunit) (Transcriptase beta chain) (RNA polymerase
           beta subunit)
          Length = 1184

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 16/67 (23%), Positives = 35/67 (52%)
 Frame = -1

Query: 271 TLLRQLFFAHPRGEDDN*HLLRGTRRIQAQGHSERLELSSEPCCHNVSHAIELSTGIRSM 92
           +L+RQ+FF   R + +     +  +R++     E++ L+ E     + + IEL+ G +++
Sbjct: 339 SLIRQMFFNPQRYDLEPVGRYKMNKRLKLDVPEEQISLTKEDVLGTIKYVIELNNGEQNV 398

Query: 91  *SKYIPN 71
            +  I N
Sbjct: 399 HTDDIDN 405



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>NU5M_ELEMA (Q2I3G4) NADH-ubiquinone oxidoreductase chain 5 (EC 1.6.5.3) (NADH|
           dehydrogenase subunit 5)
          Length = 606

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 22/54 (40%), Positives = 30/54 (55%), Gaps = 5/54 (9%)
 Frame = +2

Query: 53  PSCLHFIGDVLTLHGSYSSTQLNSMTHIVTAWFR**FQPLRM-----TLGLDPS 199
           P  L F+   +TL G   +T+LN+MTH +T  F+   QP RM     TLG  P+
Sbjct: 486 PPHLKFMALAVTLLGFTVATELNNMTHNLT--FK---QPSRMHTFSTTLGYYPT 534



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>ABCB7_HUMAN (O75027) ATP-binding cassette sub-family B member 7, mitochondrial|
           precursor (ATP-binding cassette transporter 7) (ABC
           transporter 7 protein)
          Length = 752

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 11/33 (33%), Positives = 19/33 (57%)
 Frame = -2

Query: 273 VHYSDNYFSLTPGEKMTTDISFEAPEGSKPRVI 175
           V + + +F    G+K+ + ISFE P G K  ++
Sbjct: 472 VAFDNVHFEYIEGQKVLSGISFEVPAGKKVAIV 504



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>LEG2_PIG (Q29373) Galectin-2 (Fragment)|
          Length = 123

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 14/46 (30%), Positives = 21/46 (45%)
 Frame = -2

Query: 342 LHFSVHTSESSIGENYRDTRILPVHYSDNYFSLTPGEKMTTDISFE 205
           LHF     ES+I  N RD         D++    PG ++   ++FE
Sbjct: 44  LHFXPRFGESTIVCNXRDGNXWGKEQRDSHMXFXPGSEVKLIVTFE 89



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>ABCB7_RAT (Q704E8) ATP-binding cassette sub-family B member 7, mitochondrial|
           precursor (ATP-binding cassette transporter 7) (ABC
           transporter 7 protein)
          Length = 752

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 10/33 (30%), Positives = 19/33 (57%)
 Frame = -2

Query: 273 VHYSDNYFSLTPGEKMTTDISFEAPEGSKPRVI 175
           V + + +F    G+K+ + +SFE P G K  ++
Sbjct: 472 VAFDNVHFEYIEGQKVLSGVSFEVPAGKKVAIV 504


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,727,813
Number of Sequences: 219361
Number of extensions: 1125397
Number of successful extensions: 2511
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 2380
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2508
length of database: 80,573,946
effective HSP length: 93
effective length of database: 60,173,373
effective search space used: 1444160952
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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