ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl25c10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1IF3A_SCHPO (O74760) Probable eukaryotic translation initiation f... 34 0.076
2I5P2_HUMAN (P32019) Type II inositol-1,4,5-trisphosphate 5-phosp... 31 0.64
3ROBO3_MOUSE (Q9Z2I4) Roundabout homolog 3 precursor (Retinoblast... 30 1.4
4CAC1H_MOUSE (O88427) Voltage-dependent T-type calcium channel al... 29 3.2
5PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type... 28 4.2
6MSH6_SCHPO (O74502) DNA mismatch repair protein msh6 28 4.2
7ASNS_TRIVS (O24661) Asparagine synthetase [glutamine-hydrolyzing... 28 4.2
8YPE2_RHORU (P23139) Hypothetical 25.8 kDa protein in petC 3'region 28 5.4
9SYH_MYCPA (P60917) Histidyl-tRNA synthetase (EC 6.1.1.21) (Histi... 28 5.4
10OXAA_DEIRA (Q9RSH5) Membrane protein oxaA 28 5.4
11PPDK_RHIME (Q59754) Pyruvate, phosphate dikinase (EC 2.7.9.1) (P... 28 7.1
12GLUB7_ORYSA (Q52PJ1) Glutelin type-B 7 precursor [Contains: Glut... 28 7.1
13GLUB2_ORYSA (Q02897) Glutelin type-B 2 precursor [Contains: Glut... 28 7.1
14LACB2_HORSE (P07380) Beta-lactoglobulin-2 precursor (Beta-LG-2) ... 27 9.3
15ATX3_CAEBR (Q60XN1) Ataxin-3 homolog (EC 3.4.22.-) (Machado-Jose... 27 9.3
16ISPE_SILPO (Q5LX98) 4-diphosphocytidyl-2-C-methyl-D-erythritol k... 27 9.3
17CLPB1_SYNEL (Q8DJ40) Chaperone clpB 1 27 9.3
18SMBP2_HUMAN (P38935) DNA-binding protein SMUBP-2 (EC 3.6.1.-) (A... 27 9.3
19ENAH_HUMAN (Q8N8S7) Protein enabled homolog 27 9.3

>IF3A_SCHPO (O74760) Probable eukaryotic translation initiation factor 3 110 kDa|
            subunit (eIF3 p110) (Translation initiation factor eIF3,
            p110 subunit) (eIF3a)
          Length = 932

 Score = 34.3 bits (77), Expect = 0.076
 Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
 Frame = +3

Query: 186  RTAKISPSRQEEHGAFQARKLQRLQPVEEELDEKFAEVLDP---GRQRSLNAAGAPPSGW 356
            R A+ +  R++      A KL+R + +++E DE+ +  L      R+ ++ A+   P  W
Sbjct: 825  RAAREAKEREQRE---MAEKLERQRRIQQERDEEISRKLAEKAAARRANIGASSPSPGAW 881

Query: 357  RAGGA 371
            R GGA
Sbjct: 882  RRGGA 886



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>I5P2_HUMAN (P32019) Type II inositol-1,4,5-trisphosphate 5-phosphatase|
           precursor (EC 3.1.3.36) (Phosphoinositide 5-phosphatase)
           (5PTase) (75 kDa inositol polyphosphate-5-phosphatase)
          Length = 993

 Score = 31.2 bits (69), Expect = 0.64
 Identities = 16/33 (48%), Positives = 21/33 (63%)
 Frame = -1

Query: 148 LPLVFGSPVT*ICLLRP*IFEWRLCTISQLCLM 50
           LP  FGSP+  +C +R  I +  L TIS+L LM
Sbjct: 780 LPSCFGSPIHTLCYMREPILDLPLETISELTLM 812



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>ROBO3_MOUSE (Q9Z2I4) Roundabout homolog 3 precursor (Retinoblastoma-inhibiting|
            gene 1) (Rig-1)
          Length = 1366

 Score = 30.0 bits (66), Expect = 1.4
 Identities = 16/55 (29%), Positives = 20/55 (36%)
 Frame = +2

Query: 179  WKEDGKNLTQQAGRARRISGQEASAPPASGRGVG*KICRGPRSRTPAFIKCSWST 343
            W E+    +   G      G       + GRG G + CR P S       C WST
Sbjct: 1300 WGEESGAGSASRGPTSSQRGPHPDGKESQGRGRGLEACRSPNSPQLPLDSCIWST 1354



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>CAC1H_MOUSE (O88427) Voltage-dependent T-type calcium channel alpha-1H subunit|
           (Voltage-gated calcium channel alpha subunit Cav3.2)
          Length = 2365

 Score = 28.9 bits (63), Expect = 3.2
 Identities = 12/20 (60%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
 Frame = -1

Query: 364 PARQP-DGGAPAAFNERWRP 308
           P +QP +GG P   NERWRP
Sbjct: 742 PVQQPHEGGTPGHGNERWRP 761



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>PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type-1|
           retrotransposable element R2 (Retrovirus-related Pol
           polyprotein from type I retrotransposable element R2)
           [Includes: Reverse transcriptase (EC 2.7.7.49);
           Endonuclease] (Fragment)
          Length = 606

 Score = 28.5 bits (62), Expect = 4.2
 Identities = 15/39 (38%), Positives = 22/39 (56%)
 Frame = -2

Query: 267 PLAGGAEASWPEMRRALPACWVRFLPSSFQAWELHTQLK 151
           PL  GA   WP  R  +P C +  +P++ +A  +HT LK
Sbjct: 543 PLLLGARGIWP--RANVPTCNILSIPTTLRASCVHTCLK 579



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>MSH6_SCHPO (O74502) DNA mismatch repair protein msh6|
          Length = 1254

 Score = 28.5 bits (62), Expect = 4.2
 Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 20/86 (23%)
 Frame = +3

Query: 159 VYEAPRLGRRTAKISPS--------RQEEHGAF--QARKLQRLQPVEEELDEKFAEVLDP 308
           +YE+ RLG + A  SPS        +  ++G    + ++ QR++  ++E +E++  +LD 
Sbjct: 287 LYESYRLGSQIASPSPSVSGSASPTKSNKNGVLNREEKRRQRMEAFKKENNERYEWLLDV 346

Query: 309 GRQRSLNAAG----------APPSGW 356
            R    N  G           PPS W
Sbjct: 347 -RDADQNRVGDPNYDPRTLYIPPSAW 371



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>ASNS_TRIVS (O24661) Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)|
           (Glutamine-dependent asparagine synthetase)
          Length = 585

 Score = 28.5 bits (62), Expect = 4.2
 Identities = 14/38 (36%), Positives = 18/38 (47%)
 Frame = +1

Query: 244 SFSASSQWKRSWMKNLQRS*IQDASVH*MQLEHHRPAG 357
           S + +  W  SW KNL  S      VH +  E+H P G
Sbjct: 523 STATAVAWDASWSKNLDPSGRAATGVHDLAYENHVPIG 560



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>YPE2_RHORU (P23139) Hypothetical 25.8 kDa protein in petC 3'region|
          Length = 238

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 12/18 (66%), Positives = 15/18 (83%)
 Frame = +3

Query: 291 AEVLDPGRQRSLNAAGAP 344
           A VLDPGR++SL+A G P
Sbjct: 168 AAVLDPGRKQSLSAMGLP 185



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>SYH_MYCPA (P60917) Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA|
           ligase) (HisRS)
          Length = 420

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 19/54 (35%), Positives = 23/54 (42%)
 Frame = +3

Query: 141 RGRPSIVYEAPRLGRRTAKISPSRQEEHGAFQARKLQRLQPVEEELDEKFAEVL 302
           RG    +  A R G R A +   R  + G    + LQ    V  ELD   AEVL
Sbjct: 362 RGLKGAMRAADRSGARIALVLGDRDIDAGTVGVKDLQTGSQVSVELDSVVAEVL 415



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>OXAA_DEIRA (Q9RSH5) Membrane protein oxaA|
          Length = 428

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 10/28 (35%), Positives = 20/28 (71%)
 Frame = +3

Query: 243 KLQRLQPVEEELDEKFAEVLDPGRQRSL 326
           ++Q +QP+++E+ EK+    DP  QR++
Sbjct: 241 RMQLVQPLQKEIQEKYKGKTDPESQRAM 268



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>PPDK_RHIME (Q59754) Pyruvate, phosphate dikinase (EC 2.7.9.1) (Pyruvate,|
           orthophosphate dikinase)
          Length = 898

 Score = 27.7 bits (60), Expect = 7.1
 Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 4/69 (5%)
 Frame = +3

Query: 174 RLGRRTAK----ISPSRQEEHGAFQARKLQRLQPVEEELDEKFAEVLDPGRQRSLNAAGA 341
           R G+RTAK    I+    EE    +   + R+ P    LD+     +DP  +R +  +G 
Sbjct: 352 RSGKRTAKSALKIAVDMAEEGLISKEEAVARIDPAS--LDQLLHPTIDPHARRDIIGSGL 409

Query: 342 PPSGWRAGG 368
           P S   A G
Sbjct: 410 PASPGAATG 418



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>GLUB7_ORYSA (Q52PJ1) Glutelin type-B 7 precursor [Contains: Glutelin type-B 7|
           acidic chain; Glutelin type-B 7 basic chain]
          Length = 495

 Score = 27.7 bits (60), Expect = 7.1
 Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
 Frame = +3

Query: 156 IVYEAPRLGRRTAK-ISPSRQEEHGAFQARKLQRLQP 263
           +V  A R+ R  A+ I  +R EEHGAF  R  Q+  P
Sbjct: 447 VVANAYRISREQARSIKNNRGEEHGAFTPRFQQQYYP 483



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>GLUB2_ORYSA (Q02897) Glutelin type-B 2 precursor [Contains: Glutelin type-B 2|
           acidic chain; Glutelin type-B 2 basic chain]
          Length = 495

 Score = 27.7 bits (60), Expect = 7.1
 Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
 Frame = +3

Query: 156 IVYEAPRLGRRTAK-ISPSRQEEHGAFQARKLQRLQP 263
           +V  A R+ R  A+ I  +R EEHGAF  R  Q+  P
Sbjct: 447 VVANAYRISREQARSIKNNRGEEHGAFTPRFQQQYYP 483



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>LACB2_HORSE (P07380) Beta-lactoglobulin-2 precursor (Beta-LG-2)|
           (Beta-lactoglobulin II) (BLGII) (Minor monomeric
           beta-lactoglobulin)
          Length = 181

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 204 PSRQEEHGAFQARKLQRLQPVEEELDEKFAEVLDP 308
           P    EHG    + L R Q V+EE+ EKF+  L P
Sbjct: 128 PLPSAEHGMV-CQYLARTQKVDEEVMEKFSRALQP 161



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>ATX3_CAEBR (Q60XN1) Ataxin-3 homolog (EC 3.4.22.-) (Machado-Joseph|
           disease-like protein)
          Length = 319

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
 Frame = +3

Query: 117 QVTGLPNTRGRPSIVYEAPRLGRRTAKISPSRQEEHGAFQAR-KLQRLQPVEEE 275
           Q   +P+T   PS + E P LG  T+  +PS +      Q R + + L+ +EEE
Sbjct: 257 QDPNIPSTSAAPSELTETPILGPSTSSETPSGRIPSAEQQRRDRAKFLEKLEEE 310



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>ISPE_SILPO (Q5LX98) 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC|
           2.7.1.148) (CMK)
           (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol
           kinase)
          Length = 279

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 19/53 (35%), Positives = 25/53 (47%), Gaps = 3/53 (5%)
 Frame = -2

Query: 270 LPLA---GGAEASWPEMRRALPACWVRFLPSSFQAWELHTQLKVCPWYLEARL 121
           LP+A   GG  A      RAL   W    PS+ QA  L   + VC + + AR+
Sbjct: 90  LPVASGIGGGSADAAATLRALAQLWGLAAPSAEQALTLGADVPVCLFPVPARM 142



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>CLPB1_SYNEL (Q8DJ40) Chaperone clpB 1|
          Length = 871

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 2/56 (3%)
 Frame = +3

Query: 174 RLGRRTAKISPSRQEEHGAFQARK--LQRLQPVEEELDEKFAEVLDPGRQRSLNAA 335
           +L R  A +   +   +  +QA K  + RLQ ++EE+++   E+    R   LN A
Sbjct: 444 KLERELADLKEEQSRLNAQWQAEKEVIDRLQSIKEEIEKVNIEIQQAERNYDLNRA 499



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>SMBP2_HUMAN (P38935) DNA-binding protein SMUBP-2 (EC 3.6.1.-) (ATP-dependent|
           helicase IGHMBP2) (Immunoglobulin mu-binding protein 2)
           (SMUBP-2) (Glial factor 1) (GF-1)
          Length = 993

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 14/35 (40%), Positives = 18/35 (51%)
 Frame = +2

Query: 167 SSQAWKEDGKNLTQQAGRARRISGQEASAPPASGR 271
           S  A K  G   + + G  R+  GQEA+AP   GR
Sbjct: 660 SHAATKPQGPATSTRTGSQRQEGGQEAAAPARQGR 694



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>ENAH_HUMAN (Q8N8S7) Protein enabled homolog|
          Length = 591

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 15/55 (27%), Positives = 28/55 (50%)
 Frame = +3

Query: 186 RTAKISPSRQEEHGAFQARKLQRLQPVEEELDEKFAEVLDPGRQRSLNAAGAPPS 350
           R  ++   RQE     +  +L+R +   E  ++   E L+  R+R +++A AP S
Sbjct: 218 RQERLDRERQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPAS 272


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,842,125
Number of Sequences: 219361
Number of extensions: 1348699
Number of successful extensions: 3850
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 3749
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3850
length of database: 80,573,946
effective HSP length: 99
effective length of database: 58,857,207
effective search space used: 1412572968
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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