ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl25c06
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TOP1_ARATH (P30181) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 75 9e-14
2TOP1_DAUCA (P93119) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 68 9e-12
3TOP1_DROME (P30189) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 54 2e-07
4TOP1_HUMAN (P11387) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 52 5e-07
5TOP1_RAT (Q9WUL0) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoiso... 52 5e-07
6TOP1_MOUSE (Q04750) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 52 5e-07
7TOP1_CRIGR (Q07050) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 52 5e-07
8TOP1_CERAE (Q7YR26) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 52 5e-07
9TOP1_SCHPO (P07799) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 50 2e-06
10TOP1_YEAST (P04786) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 49 5e-06
11TOP1M_HUMAN (Q969P6) DNA topoisomerase I, mitochondrial precurso... 49 7e-06
12TOP1_XENLA (P41512) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 40 0.003
13TOP1_CANAL (Q00313) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoi... 40 0.003
14TAF7_YEAST (Q05021) Transcription initiation factor TFIID subuni... 35 0.11
15PP1G_XENLA (P36874) Serine/threonine-protein phosphatase PP1-gam... 29 5.8
16PP1G_RAT (P63088) Serine/threonine-protein phosphatase PP1-gamma... 29 5.8
17PP1G_MOUSE (P63087) Serine/threonine-protein phosphatase PP1-gam... 29 5.8
18PP1G_HUMAN (P36873) Serine/threonine-protein phosphatase PP1-gam... 29 5.8
19PP1G_CANFA (Q8MJ46) Serine/threonine-protein phosphatase PP1-gam... 29 5.8
20PP1G_BOVIN (P61287) Serine/threonine-protein phosphatase PP1-gam... 29 5.8
21DNLJ_THEFI (Q9ZHI0) DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleo... 29 5.8
22CASP2_HUMAN (P42575) Caspase-2 precursor (EC 3.4.22.-) (CASP-2) ... 28 7.6
23IFRH_ARATH (P52577) Isoflavone reductase homolog P3 (EC 1.3.1.-) 28 7.6

>TOP1_ARATH (P30181) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 916

 Score = 74.7 bits (182), Expect = 9e-14
 Identities = 31/39 (79%), Positives = 36/39 (92%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDVEPDFRF 321
           PRITVAWCK HEVPIEKIF+K++L KF WAMDVEP++RF
Sbjct: 875 PRITVAWCKRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 913



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>TOP1_DAUCA (P93119) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 790

 Score = 68.2 bits (165), Expect = 9e-12
 Identities = 30/39 (76%), Positives = 34/39 (87%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDVEPDFRF 321
           PRITVAWCK H+VPIEKIF+K++LAKF WAMDV   FRF
Sbjct: 752 PRITVAWCKRHDVPIEKIFNKSLLAKFAWAMDVVLIFRF 790



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>TOP1_DROME (P30189) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 972

 Score = 53.5 bits (127), Expect = 2e-07
 Identities = 24/40 (60%), Positives = 32/40 (80%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437  PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDV-EPDFRF 321
            PRI+VAWCK H+VPIEKIF+KT   KF WA+ + + ++RF
Sbjct: 933  PRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 972



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>TOP1_HUMAN (P11387) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 765

 Score = 52.4 bits (124), Expect = 5e-07
 Identities = 24/40 (60%), Positives = 30/40 (75%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDV-EPDFRF 321
           PRITVAWCK   VPIEKI++KT   KF WA+D+ + D+ F
Sbjct: 726 PRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF 765



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>TOP1_RAT (Q9WUL0) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 767

 Score = 52.4 bits (124), Expect = 5e-07
 Identities = 24/40 (60%), Positives = 30/40 (75%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDV-EPDFRF 321
           PRITVAWCK   VPIEKI++KT   KF WA+D+ + D+ F
Sbjct: 728 PRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF 767



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>TOP1_MOUSE (Q04750) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 767

 Score = 52.4 bits (124), Expect = 5e-07
 Identities = 24/40 (60%), Positives = 30/40 (75%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDV-EPDFRF 321
           PRITVAWCK   VPIEKI++KT   KF WA+D+ + D+ F
Sbjct: 728 PRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF 767



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>TOP1_CRIGR (Q07050) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 767

 Score = 52.4 bits (124), Expect = 5e-07
 Identities = 24/40 (60%), Positives = 30/40 (75%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDV-EPDFRF 321
           PRITVAWCK   VPIEKI++KT   KF WA+D+ + D+ F
Sbjct: 728 PRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF 767



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>TOP1_CERAE (Q7YR26) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 767

 Score = 52.4 bits (124), Expect = 5e-07
 Identities = 24/40 (60%), Positives = 30/40 (75%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDV-EPDFRF 321
           PRITVAWCK   VPIEKI++KT   KF WA+D+ + D+ F
Sbjct: 728 PRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF 767



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>TOP1_SCHPO (P07799) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 814

 Score = 50.4 bits (119), Expect = 2e-06
 Identities = 21/39 (53%), Positives = 29/39 (74%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDVEPDFRF 321
           PR+T ++ K  +VPIEK+FSKTI  KF WA D  PD+++
Sbjct: 776 PRLTYSFSKREDVPIEKLFSKTIRDKFNWAADTPPDWKW 814



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>TOP1_YEAST (P04786) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
           (Maintenance of killer protein 1)
          Length = 769

 Score = 48.9 bits (115), Expect = 5e-06
 Identities = 21/40 (52%), Positives = 33/40 (82%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMD-VEPDFRF 321
           PR++V +CK ++VPIEKIF+KT+  KF WA++ V+ ++RF
Sbjct: 730 PRLSVVFCKKYDVPIEKIFTKTLREKFKWAIESVDENWRF 769



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>TOP1M_HUMAN (Q969P6) DNA topoisomerase I, mitochondrial precursor (EC 5.99.1.2)|
           (TOP1mt)
          Length = 601

 Score = 48.5 bits (114), Expect = 7e-06
 Identities = 21/40 (52%), Positives = 28/40 (70%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMDVE-PDFRF 321
           PRI++AWCK   VP+EKI+SKT   +F WA+ +   DF F
Sbjct: 562 PRISIAWCKRFRVPVEKIYSKTQRERFAWALAMAGEDFEF 601



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>TOP1_XENLA (P41512) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 829

 Score = 40.0 bits (92), Expect = 0.003
 Identities = 16/22 (72%), Positives = 20/22 (90%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKT 372
           PRI+VAWCK + VPIEKI++KT
Sbjct: 782 PRISVAWCKKYGVPIEKIYNKT 803



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>TOP1_CANAL (Q00313) DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)|
          Length = 778

 Score = 39.7 bits (91), Expect = 0.003
 Identities = 18/40 (45%), Positives = 29/40 (72%), Gaps = 1/40 (2%)
 Frame = -1

Query: 437 PRITVAWCKTHEVPIEKIFSKTILAKFGWAMD-VEPDFRF 321
           PR+ V + K  +VPIEK+F+KT+  KF WA++  + ++RF
Sbjct: 739 PRLIVMFSKKFDVPIEKLFTKTLREKFIWAIESADENWRF 778



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>TAF7_YEAST (Q05021) Transcription initiation factor TFIID subunit 7|
           (TBP-associated factor 7) (TBP-associated factor 67 kDa)
           (TAFII-67)
          Length = 590

 Score = 34.7 bits (78), Expect = 0.11
 Identities = 33/121 (27%), Positives = 57/121 (47%)
 Frame = +3

Query: 75  RPKCKHEFRQSDHSKYGREDG*KKSLQRECRVLPSSRSRETSKNKHRALPLLLVAVRCPG 254
           +PK K   ++   S  G+E   K SL+ +  +    ++ E  K  H+A  L L  +R PG
Sbjct: 42  KPKLKINLKKKKESADGKEK--KNSLKLKLNL---KKNEEPVKKIHKAPKLRLKPIRIPG 96

Query: 255 EGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLCKNRLAENLLNRDFMGFAPCYRNS 434
           E  ++E++      LI++ ++L   I  DI    +  KN L       D+ G +  ++N 
Sbjct: 97  EAYDSEASDIEDDPLIESGVIL--RILPDIQL--EFVKNSLESG----DYSGISIKWKNE 148

Query: 435 R 437
           R
Sbjct: 149 R 149



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>PP1G_XENLA (P36874) Serine/threonine-protein phosphatase PP1-gamma catalytic|
           subunit (EC 3.1.3.16) (PP-1G)
          Length = 323

 Score = 28.9 bits (63), Expect = 5.8
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = +3

Query: 225 LLLVAVRCPGEGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLC 365
           LL V    PG+ ++ + N  RG  L    I L   I L++  P K+C
Sbjct: 16  LLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKIC 62



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>PP1G_RAT (P63088) Serine/threonine-protein phosphatase PP1-gamma catalytic|
           subunit (EC 3.1.3.16) (PP-1G) (Protein phosphatase 1C
           catalytic subunit)
          Length = 323

 Score = 28.9 bits (63), Expect = 5.8
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = +3

Query: 225 LLLVAVRCPGEGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLC 365
           LL V    PG+ ++ + N  RG  L    I L   I L++  P K+C
Sbjct: 16  LLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKIC 62



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>PP1G_MOUSE (P63087) Serine/threonine-protein phosphatase PP1-gamma catalytic|
           subunit (EC 3.1.3.16) (PP-1G) (Protein phosphatase 1C
           catalytic subunit)
          Length = 323

 Score = 28.9 bits (63), Expect = 5.8
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = +3

Query: 225 LLLVAVRCPGEGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLC 365
           LL V    PG+ ++ + N  RG  L    I L   I L++  P K+C
Sbjct: 16  LLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKIC 62



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>PP1G_HUMAN (P36873) Serine/threonine-protein phosphatase PP1-gamma catalytic|
           subunit (EC 3.1.3.16) (PP-1G) (Protein phosphatase 1C
           catalytic subunit)
          Length = 323

 Score = 28.9 bits (63), Expect = 5.8
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = +3

Query: 225 LLLVAVRCPGEGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLC 365
           LL V    PG+ ++ + N  RG  L    I L   I L++  P K+C
Sbjct: 16  LLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKIC 62



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>PP1G_CANFA (Q8MJ46) Serine/threonine-protein phosphatase PP1-gamma catalytic|
           subunit (EC 3.1.3.16) (PP-1G) (Protein phosphatase 1C
           catalytic subunit)
          Length = 323

 Score = 28.9 bits (63), Expect = 5.8
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = +3

Query: 225 LLLVAVRCPGEGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLC 365
           LL V    PG+ ++ + N  RG  L    I L   I L++  P K+C
Sbjct: 16  LLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKIC 62



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>PP1G_BOVIN (P61287) Serine/threonine-protein phosphatase PP1-gamma catalytic|
           subunit (EC 3.1.3.16) (PP-1G) (Protein phosphatase 1C
           catalytic subunit)
          Length = 323

 Score = 28.9 bits (63), Expect = 5.8
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = +3

Query: 225 LLLVAVRCPGEGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLC 365
           LL V    PG+ ++ + N  RG  L    I L   I L++  P K+C
Sbjct: 16  LLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKIC 62



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>DNLJ_THEFI (Q9ZHI0) DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase|
           [NAD+]) (Tfi DNA ligase)
          Length = 670

 Score = 28.9 bits (63), Expect = 5.8
 Identities = 20/72 (27%), Positives = 31/72 (43%)
 Frame = +3

Query: 189 RETSKNKHRALPLLLVAVRCPGEGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLCK 368
           R+  ++KHR L  LL A+  PG G     NL R    +   +    E  L++    +L  
Sbjct: 496 RQIEESKHRGLERLLYALGLPGVGEVLARNLARRFGTMDRLLEASLEELLEVEEVGELTA 555

Query: 369 NRLAENLLNRDF 404
             + E L +  F
Sbjct: 556 RAILETLKDPAF 567



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>CASP2_HUMAN (P42575) Caspase-2 precursor (EC 3.4.22.-) (CASP-2) (ICH-1|
           protease) (ICH-1L/1S) [Contains: Caspase-2 subunit p18;
           Caspase-2 subunit p13; Caspase-2 subunit p12]
          Length = 452

 Score = 28.5 bits (62), Expect = 7.6
 Identities = 19/55 (34%), Positives = 25/55 (45%)
 Frame = +3

Query: 255 EGLEAESNLPRGHWLIQNPILLESEIRLDIHCPSKLCKNRLAENLLNRDFMGFAP 419
           +G  A  N  RG W I+    + SE   D+H    L K     N L +D  G+AP
Sbjct: 372 KGTAAMRNTKRGSWYIEALAQVFSERACDMHVADMLVK----VNALIKDREGYAP 422



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>IFRH_ARATH (P52577) Isoflavone reductase homolog P3 (EC 1.3.1.-)|
          Length = 310

 Score = 28.5 bits (62), Expect = 7.6
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = +1

Query: 46  PSVLNKLKFVGPNANTSLGNRTIASTEEKMGK 141
           P  LNK+ ++ P+ NT   N  +   E+K+GK
Sbjct: 208 PRTLNKILYIKPSNNTLSMNEIVTLWEKKIGK 239


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,344,517
Number of Sequences: 219361
Number of extensions: 1363246
Number of successful extensions: 3199
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 3159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3198
length of database: 80,573,946
effective HSP length: 101
effective length of database: 58,418,485
effective search space used: 2570413340
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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