Clone Name | rbastl21a08 |
---|---|
Clone Library Name | barley_pub |
>dbj|AP008218.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 12, complete sequence Length = 27566993 Score = 216 bits (109), Expect = 4e-53 Identities = 220/257 (85%) Strand = Plus / Minus Query: 159 gcttcaccaaacccgtaggagctgaagtgcttcactctgaacttccactcgcccttggcg 218 ||||||||||| || ||||||||||| ||||| || ||||| ||||||||||||||||| Sbjct: 3343074 gcttcaccaaagccataggagctgaaatgcttgaccctgaatttccactcgcccttggca 3343015 Query: 219 ccatcaaacgagataaactccactccctgttcctcagccttcttcaccagcatctccttg 278 |||| || ||||| ||||| | ||||| |||||||||||||||||||| ||||||||| Sbjct: 3343014 gcatcgaatgagatgaactcagcaccctgctcctcagccttcttcaccagtatctccttg 3342955 Query: 279 aacttgtcaaacctcggaccttgcgtgtactgctcgtccgtcttcccattcatgcacttg 338 ||||| | | ||||||||| | ||||||||||| |||||||| |||||||||||| Sbjct: 3342954 tacttgcccaccctcggaccctctgtgtactgctcccccgtcttcttgttcatgcacttg 3342895 Query: 339 atgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactgggggcttcttg 398 || || || |||||||||| |||||||| ||||| ||||| ||||| |||||||||||| Sbjct: 3342894 atattgagaagggtcaccacggcagctttattcagaccctcaccaacagggggcttcttg 3342835 Query: 399 ctatcgtccttgtacac 415 ||||||||||||||||| Sbjct: 3342834 ctatcgtccttgtacac 3342818 Score = 208 bits (105), Expect = 1e-50 Identities = 219/257 (85%) Strand = Plus / Minus Query: 159 gcttcaccaaacccgtaggagctgaagtgcttcactctgaacttccactcgcccttggcg 218 ||||||||||| || ||||||||||| ||||| || ||||| ||||||||||| ||| || Sbjct: 3351809 gcttcaccaaagccataggagctgaaatgcttgaccctgaatttccactcgccattgacg 3351750 Query: 219 ccatcaaacgagataaactccactccctgttcctcagccttcttcaccagcatctccttg 278 |||| || ||||| ||||| | ||||| |||||||| ||||||||||||||||||||| Sbjct: 3351749 gcatcgaaagagatgaactcagcaccctgctcctcagctttcttcaccagcatctccttg 3351690 Query: 279 aacttgtcaaacctcggaccttgcgtgtactgctcgtccgtcttcccattcatgcacttg 338 ||||||| | ||||||||| | |||||||| || |||||||| |||||||||||| Sbjct: 3351689 tacttgtccaccctcggaccctctgtgtactgatcccccgtcttcttgttcatgcacttg 3351630 Query: 339 atgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactgggggcttcttg 398 || || || |||||||||| |||||||| ||||| ||||| ||||| |||||||||||| Sbjct: 3351629 atattgagaagggtcaccacggcagctttattcagaccctcaccaacagggggcttcttg 3351570 Query: 399 ctatcgtccttgtacac 415 ||||||||||||||||| Sbjct: 3351569 ctatcgtccttgtacac 3351553
>gb|DP000011.1| Oryza sativa (japonica cultivar-group) chromosome 12, complete sequence Length = 27492551 Score = 216 bits (109), Expect = 4e-53 Identities = 220/257 (85%) Strand = Plus / Minus Query: 159 gcttcaccaaacccgtaggagctgaagtgcttcactctgaacttccactcgcccttggcg 218 ||||||||||| || ||||||||||| ||||| || ||||| ||||||||||||||||| Sbjct: 3343019 gcttcaccaaagccataggagctgaaatgcttgaccctgaatttccactcgcccttggca 3342960 Query: 219 ccatcaaacgagataaactccactccctgttcctcagccttcttcaccagcatctccttg 278 |||| || ||||| ||||| | ||||| |||||||||||||||||||| ||||||||| Sbjct: 3342959 gcatcgaatgagatgaactcagcaccctgctcctcagccttcttcaccagtatctccttg 3342900 Query: 279 aacttgtcaaacctcggaccttgcgtgtactgctcgtccgtcttcccattcatgcacttg 338 ||||| | | ||||||||| | ||||||||||| |||||||| |||||||||||| Sbjct: 3342899 tacttgcccaccctcggaccctctgtgtactgctcccccgtcttcttgttcatgcacttg 3342840 Query: 339 atgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactgggggcttcttg 398 || || || |||||||||| |||||||| ||||| ||||| ||||| |||||||||||| Sbjct: 3342839 atattgagaagggtcaccacggcagctttattcagaccctcaccaacagggggcttcttg 3342780 Query: 399 ctatcgtccttgtacac 415 ||||||||||||||||| Sbjct: 3342779 ctatcgtccttgtacac 3342763 Score = 208 bits (105), Expect = 1e-50 Identities = 219/257 (85%) Strand = Plus / Minus Query: 159 gcttcaccaaacccgtaggagctgaagtgcttcactctgaacttccactcgcccttggcg 218 ||||||||||| || ||||||||||| ||||| || ||||| ||||||||||| ||| || Sbjct: 3351754 gcttcaccaaagccataggagctgaaatgcttgaccctgaatttccactcgccattgacg 3351695 Query: 219 ccatcaaacgagataaactccactccctgttcctcagccttcttcaccagcatctccttg 278 |||| || ||||| ||||| | ||||| |||||||| ||||||||||||||||||||| Sbjct: 3351694 gcatcgaaagagatgaactcagcaccctgctcctcagctttcttcaccagcatctccttg 3351635 Query: 279 aacttgtcaaacctcggaccttgcgtgtactgctcgtccgtcttcccattcatgcacttg 338 ||||||| | ||||||||| | |||||||| || |||||||| |||||||||||| Sbjct: 3351634 tacttgtccaccctcggaccctctgtgtactgatcccccgtcttcttgttcatgcacttg 3351575 Query: 339 atgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactgggggcttcttg 398 || || || |||||||||| |||||||| ||||| ||||| ||||| |||||||||||| Sbjct: 3351574 atattgagaagggtcaccacggcagctttattcagaccctcaccaacagggggcttcttg 3351515 Query: 399 ctatcgtccttgtacac 415 ||||||||||||||||| Sbjct: 3351514 ctatcgtccttgtacac 3351498
>emb|AL844874.1|CNS08CAX Oryza sativa chromosome 12, . BAC OSJNBa0041K23 of library OSJNBa from chromosome 12 of cultivar Nipponbare of ssp. japonica of Oryza sativa (rice), complete sequence Length = 137936 Score = 216 bits (109), Expect = 4e-53 Identities = 220/257 (85%) Strand = Plus / Minus Query: 159 gcttcaccaaacccgtaggagctgaagtgcttcactctgaacttccactcgcccttggcg 218 ||||||||||| || ||||||||||| ||||| || ||||| ||||||||||||||||| Sbjct: 22127 gcttcaccaaagccataggagctgaaatgcttgaccctgaatttccactcgcccttggca 22068 Query: 219 ccatcaaacgagataaactccactccctgttcctcagccttcttcaccagcatctccttg 278 |||| || ||||| ||||| | ||||| |||||||||||||||||||| ||||||||| Sbjct: 22067 gcatcgaatgagatgaactcagcaccctgctcctcagccttcttcaccagtatctccttg 22008 Query: 279 aacttgtcaaacctcggaccttgcgtgtactgctcgtccgtcttcccattcatgcacttg 338 ||||| | | ||||||||| | ||||||||||| |||||||| |||||||||||| Sbjct: 22007 tacttgcccaccctcggaccctctgtgtactgctcccccgtcttcttgttcatgcacttg 21948 Query: 339 atgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactgggggcttcttg 398 || || || |||||||||| |||||||| ||||| ||||| ||||| |||||||||||| Sbjct: 21947 atattgagaagggtcaccacggcagctttattcagaccctcaccaacagggggcttcttg 21888 Query: 399 ctatcgtccttgtacac 415 ||||||||||||||||| Sbjct: 21887 ctatcgtccttgtacac 21871 Score = 208 bits (105), Expect = 1e-50 Identities = 219/257 (85%) Strand = Plus / Minus Query: 159 gcttcaccaaacccgtaggagctgaagtgcttcactctgaacttccactcgcccttggcg 218 ||||||||||| || ||||||||||| ||||| || ||||| ||||||||||| ||| || Sbjct: 30862 gcttcaccaaagccataggagctgaaatgcttgaccctgaatttccactcgccattgacg 30803 Query: 219 ccatcaaacgagataaactccactccctgttcctcagccttcttcaccagcatctccttg 278 |||| || ||||| ||||| | ||||| |||||||| ||||||||||||||||||||| Sbjct: 30802 gcatcgaaagagatgaactcagcaccctgctcctcagctttcttcaccagcatctccttg 30743 Query: 279 aacttgtcaaacctcggaccttgcgtgtactgctcgtccgtcttcccattcatgcacttg 338 ||||||| | ||||||||| | |||||||| || |||||||| |||||||||||| Sbjct: 30742 tacttgtccaccctcggaccctctgtgtactgatcccccgtcttcttgttcatgcacttg 30683 Query: 339 atgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactgggggcttcttg 398 || || || |||||||||| |||||||| ||||| ||||| ||||| |||||||||||| Sbjct: 30682 atattgagaagggtcaccacggcagctttattcagaccctcaccaacagggggcttcttg 30623 Query: 399 ctatcgtccttgtacac 415 ||||||||||||||||| Sbjct: 30622 ctatcgtccttgtacac 30606
>gb|BT018715.1| Zea mays clone EL01N0517E03.d mRNA sequence Length = 1871 Score = 97.6 bits (49), Expect = 3e-17 Identities = 76/85 (89%) Strand = Plus / Minus Query: 332 gcacttgatgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactggggg 391 ||||||||||||||| || |||||| ||||| |||||||| |||||||| || ||||| Sbjct: 1410 gcacttgatgttcagtagagtcacctctgcagccttgttcagcccctcgcccacaggggg 1351 Query: 392 cttcttgctatcgtccttgtacaca 416 ||||||||||||||||||||||||| Sbjct: 1350 cttcttgctatcgtccttgtacaca 1326 Score = 87.7 bits (44), Expect = 3e-14 Identities = 99/116 (85%), Gaps = 1/116 (0%) Strand = Plus / Minus Query: 161 ttcaccaaa-cccgtaggagctgaagtgcttcactctgaacttccactcgcccttggcgc 219 ||||||||| |||||||| ||||||||||||||| |||||||||||||| |||||| | Sbjct: 1579 ttcaccaaagcccgtaggcgctgaagtgcttcaccctgaacttccactctcccttgacag 1520 Query: 220 catcaaacgagataaactccactccctgttcctcagccttcttcaccagcatctcc 275 | || || || | |||||| | ||||| ||||| ||||||||||||||||||||| Sbjct: 1519 cgtcgaaggacacaaactctgcgccctgctcctccgccttcttcaccagcatctcc 1464
>gb|BT018481.1| Zea mays clone EL01N0424C07.d mRNA sequence Length = 1900 Score = 91.7 bits (46), Expect = 2e-15 Identities = 88/102 (86%) Strand = Plus / Minus Query: 174 taggagctgaagtgcttcactctgaacttccactcgcccttggcgccatcaaacgagata 233 |||| |||||| |||||||| |||||||||||||| |||||||| ||||||| || | | Sbjct: 1730 taggcgctgaaatgcttcaccctgaacttccactctcccttggcagcatcaaaggacaca 1671 Query: 234 aactccactccctgttcctcagccttcttcaccagcatctcc 275 |||||| | ||||| ||||| |||||||||| |||||||||| Sbjct: 1670 aactccgcgccctgctcctccgccttcttcatcagcatctcc 1629 Score = 65.9 bits (33), Expect = 1e-07 Identities = 69/81 (85%) Strand = Plus / Minus Query: 332 gcacttgatgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactggggg 391 ||||||||||||||| || |||||| ||||| |||||||| ||||| || || ||||| Sbjct: 1572 gcacttgatgttcagtagagtcacctccgcagccttgttcagcccctcacccacaggggg 1513 Query: 392 cttcttgctatcgtccttgta 412 |||||| || ||||||||||| Sbjct: 1512 cttcttcctgtcgtccttgta 1492
>gb|AY110896.1| Zea mays CL23032_-2 mRNA sequence Length = 625 Score = 65.9 bits (33), Expect = 1e-07 Identities = 48/53 (90%) Strand = Plus / Plus Query: 174 taggagctgaagtgcttcactctgaacttccactcgcccttggcgccatcaaa 226 |||| ||||||||||||||| |||||||||||||| |||||||| ||||||| Sbjct: 184 taggcgctgaagtgcttcaccctgaacttccactctcccttggcagcatcaaa 236 Score = 44.1 bits (22), Expect = 0.36 Identities = 65/81 (80%) Strand = Plus / Plus Query: 332 gcacttgatgttcaggagggtcaccaaagcagctttgttcaggccctcgccaactggggg 391 ||||||||||||||| || |||||| ||||| |||||||| ||||| || || Sbjct: 342 gcacttgatgttcagtagagtcacctccgcagccttgttcagcccctcacccacannnnn 401 Query: 392 cttcttgctatcgtccttgta 412 ||||||||| ||||||||||| Sbjct: 402 cttcttgctgtcgtccttgta 422
>gb|AY389582.1| Hyacinthus orientalis nucleoporin mRNA, partial cds Length = 732 Score = 54.0 bits (27), Expect = 4e-04 Identities = 30/31 (96%) Strand = Plus / Minus Query: 178 agctgaagtgcttcactctgaacttccactc 208 |||||||||||||||| |||||||||||||| Sbjct: 418 agctgaagtgcttcaccctgaacttccactc 388
>gb|AC095044.3| Homo sapiens BAC clone RP11-132B16 from 7, complete sequence Length = 134184 Score = 44.1 bits (22), Expect = 0.36 Identities = 22/22 (100%) Strand = Plus / Minus Query: 244 cctgttcctcagccttcttcac 265 |||||||||||||||||||||| Sbjct: 5906 cctgttcctcagccttcttcac 5885
>gb|AC142295.1| Pan troglodytes BAC clone RP43-80F20 from 7, complete sequence Length = 165071 Score = 44.1 bits (22), Expect = 0.36 Identities = 22/22 (100%) Strand = Plus / Minus Query: 244 cctgttcctcagccttcttcac 265 |||||||||||||||||||||| Sbjct: 140564 cctgttcctcagccttcttcac 140543
>ref|XM_639988.1| Dictyostelium discoideum hypothetical protein (DDB0168011), partial mRNA Length = 4611 Score = 42.1 bits (21), Expect = 1.4 Identities = 27/29 (93%) Strand = Plus / Plus Query: 86 atcatccaaaagaggcagcaattacaaca 114 ||||||||||||| |||| |||||||||| Sbjct: 494 atcatccaaaagatgcagaaattacaaca 522
>ref|XM_639242.1| Dictyostelium discoideum hypothetical protein (DDB0217388), partial mRNA Length = 4611 Score = 42.1 bits (21), Expect = 1.4 Identities = 27/29 (93%) Strand = Plus / Plus Query: 86 atcatccaaaagaggcagcaattacaaca 114 ||||||||||||| |||| |||||||||| Sbjct: 494 atcatccaaaagatgcagaaattacaaca 522
>gb|AC155231.2| Mus musculus BAC clone RP24-336I23 from chromosome 12, complete sequence Length = 175296 Score = 42.1 bits (21), Expect = 1.4 Identities = 24/25 (96%) Strand = Plus / Plus Query: 175 aggagctgaagtgcttcactctgaa 199 |||||||| |||||||||||||||| Sbjct: 135270 aggagctgtagtgcttcactctgaa 135294
>gb|AC116984.2| Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence Length = 541399 Score = 42.1 bits (21), Expect = 1.4 Identities = 27/29 (93%) Strand = Plus / Minus Query: 86 atcatccaaaagaggcagcaattacaaca 114 ||||||||||||| |||| |||||||||| Sbjct: 178831 atcatccaaaagatgcagaaattacaaca 178803
>emb|CT009613.14| Mouse DNA sequence from clone RP23-55L9 on chromosome 12, complete sequence Length = 214861 Score = 42.1 bits (21), Expect = 1.4 Identities = 24/25 (96%) Strand = Plus / Minus Query: 175 aggagctgaagtgcttcactctgaa 199 |||||||| |||||||||||||||| Sbjct: 206265 aggagctgtagtgcttcactctgaa 206241
>gb|AC101722.14| Mus musculus chromosome 5, clone RP23-310L24, complete sequence Length = 172370 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Plus Query: 245 ctgttcctcagccttcttca 264 |||||||||||||||||||| Sbjct: 121775 ctgttcctcagccttcttca 121794
>gb|CP000352.1| Ralstonia metallidurans CH34, complete genome Length = 3928089 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Minus Query: 271 tctccttgaacttgtcaaac 290 |||||||||||||||||||| Sbjct: 509945 tctccttgaacttgtcaaac 509926
>gb|AC110034.8| Mus musculus chromosome 3, clone RP23-237L12, complete sequence Length = 188908 Score = 40.1 bits (20), Expect = 5.6 Identities = 29/32 (90%) Strand = Plus / Minus Query: 235 actccactccctgttcctcagccttcttcacc 266 |||||| | |||| |||||||||||||||||| Sbjct: 134888 actccagtacctgctcctcagccttcttcacc 134857
>gb|AE004177.1| Vibrio cholerae O1 biovar eltor str. N16961 chromosome I, section 85 of 251 of the complete chromosome Length = 21916 Score = 40.1 bits (20), Expect = 5.6 Identities = 23/24 (95%) Strand = Plus / Plus Query: 4 agaccaaaagcatcacaaccaata 27 |||||||||||||| ||||||||| Sbjct: 15310 agaccaaaagcatcccaaccaata 15333
>gb|AC161535.5| Mus musculus chromosome 5, clone RP23-26J13, complete sequence Length = 209259 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Minus Query: 245 ctgttcctcagccttcttca 264 |||||||||||||||||||| Sbjct: 202087 ctgttcctcagccttcttca 202068
>emb|AL590542.4| Human DNA sequence from clone RP11-135F12 on chromosome 6, complete sequence Length = 43345 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Minus Query: 268 gcatctccttgaacttgtca 287 |||||||||||||||||||| Sbjct: 1794 gcatctccttgaacttgtca 1775
>emb|BX842242.1| Homo sapiens chromosome 10 clone RP11-436O19, partial sequence Length = 111002 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Plus Query: 6 accaaaagcatcacaaccaa 25 |||||||||||||||||||| Sbjct: 101028 accaaaagcatcacaaccaa 101047
>emb|BX510311.6| Zebrafish DNA sequence from clone CH211-235F16, complete sequence Length = 198245 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Minus Query: 389 gggcttcttgctatcgtcct 408 |||||||||||||||||||| Sbjct: 27829 gggcttcttgctatcgtcct 27810
>emb|BX647627.1|HSM807773 Homo sapiens mRNA; cDNA DKFZp686N04110 (from clone DKFZp686N04110) Length = 8618 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Minus Query: 6 accaaaagcatcacaaccaa 25 |||||||||||||||||||| Sbjct: 1640 accaaaagcatcacaaccaa 1621
>gb|AC182810.1| Gasterosteus aculeatus clone VMRC28-119K03, complete sequence Length = 131913 Score = 40.1 bits (20), Expect = 5.6 Identities = 23/24 (95%) Strand = Plus / Minus Query: 392 cttcttgctatcgtccttgtacac 415 ||||||||| |||||||||||||| Sbjct: 20028 cttcttgctgtcgtccttgtacac 20005
>gb|AC156552.10| Mus musculus chromosome 3, clone RP24-271K21, complete sequence Length = 168716 Score = 40.1 bits (20), Expect = 5.6 Identities = 29/32 (90%) Strand = Plus / Plus Query: 235 actccactccctgttcctcagccttcttcacc 266 |||||| | |||| |||||||||||||||||| Sbjct: 168205 actccagtacctgctcctcagccttcttcacc 168236
>gb|AC002359.1|AC002359 Homo sapiens Xp22 Cosmid U239B3 (from Lawrence Livermore X library) complete sequence Length = 16699 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Plus Query: 138 tcagccaggtggtcggcttc 157 |||||||||||||||||||| Sbjct: 8656 tcagccaggtggtcggcttc 8675
>dbj|AP002980.2| Homo sapiens genomic DNA, chromosome 8q23, clone: KB1058D3 Length = 166683 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Plus Query: 326 attcatgcacttgatgttca 345 |||||||||||||||||||| Sbjct: 104715 attcatgcacttgatgttca 104734
>emb|CR456628.8| Zebrafish DNA sequence from clone CH211-157H7 in linkage group 1, complete sequence Length = 168101 Score = 40.1 bits (20), Expect = 5.6 Identities = 20/20 (100%) Strand = Plus / Plus Query: 389 gggcttcttgctatcgtcct 408 |||||||||||||||||||| Sbjct: 96954 gggcttcttgctatcgtcct 96973
>gb|L28808.1|RABMSD Oryctolagus cuniculus manganese superoxide dismutase mRNA, partial cds Length = 606 Score = 40.1 bits (20), Expect = 5.6 Identities = 23/24 (95%) Strand = Plus / Minus Query: 266 cagcatctccttgaacttgtcaaa 289 |||| ||||||||||||||||||| Sbjct: 348 cagcctctccttgaacttgtcaaa 325 Database: nt Posted date: May 29, 2006 11:10 AM Number of letters in database: 3,984,495,279 Number of sequences in database: 917,343 Database: /shigen/export/home/twatanab/db/nt/nt.01 Posted date: May 29, 2006 11:16 AM Number of letters in database: 3,988,174,986 Number of sequences in database: 835,257 Database: /shigen/export/home/twatanab/db/nt/nt.02 Posted date: May 29, 2006 11:21 AM Number of letters in database: 3,991,246,324 Number of sequences in database: 771,481 Database: /shigen/export/home/twatanab/db/nt/nt.03 Posted date: May 29, 2006 11:27 AM Number of letters in database: 3,990,718,311 Number of sequences in database: 977,174 Database: /shigen/export/home/twatanab/db/nt/nt.04 Posted date: May 29, 2006 11:29 AM Number of letters in database: 1,278,410,368 Number of sequences in database: 400,813 Lambda K H 1.37 0.711 1.31 Gapped Lambda K H 1.37 0.711 1.31 Matrix: blastn matrix:1 -3 Gap Penalties: Existence: 5, Extension: 2 Number of Hits to DB: 3,362,841 Number of Sequences: 3902068 Number of extensions: 3362841 Number of successful extensions: 63419 Number of sequences better than 10.0: 29 Number of HSP's better than 10.0 without gapping: 29 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 63325 Number of HSP's gapped (non-prelim): 94 length of query: 416 length of database: 17,233,045,268 effective HSP length: 22 effective length of query: 394 effective length of database: 17,147,199,772 effective search space: 6755996710168 effective search space used: 6755996710168 T: 0 A: 0 X1: 6 (11.9 bits) X2: 15 (29.7 bits) S1: 12 (24.3 bits) S2: 20 (40.1 bits)