ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl18h12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1CERK1_HUMAN (Q8TCT0) Ceramide kinase (EC 2.7.1.138) (Acylsphingo... 28 5.6
2NAS38_CAEEL (Q20942) Zinc metalloproteinase nas-38 precursor (EC... 28 7.4
3PCX1_HUMAN (Q96RV3) Pecanex-like protein 1 (Pecanex homolog) 28 7.4
4PGH1_RAT (Q63921) Prostaglandin G/H synthase 1 precursor (EC 1.1... 27 9.6
5T2K1_KLEPN (P25237) Type II restriction enzyme KpnI (EC 3.1.21.4... 27 9.6

>CERK1_HUMAN (Q8TCT0) Ceramide kinase (EC 2.7.1.138) (Acylsphingosine kinase)|
           (hCERK) (Lipid kinase 4) (LK4)
          Length = 537

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 18/72 (25%), Positives = 26/72 (36%), Gaps = 14/72 (19%)
 Frame = +1

Query: 64  MMGTSTETDIEGAGHNELAMKQ--------------KSSQVNRWSFYPKNDHCGLKEKQL 201
           ++G S   D+    HN   ++               K S+  RW    + D  GLK    
Sbjct: 262 VVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLKTFLS 321

Query: 202 QVCSSGTTALLP 237
             C  GT + LP
Sbjct: 322 HHCYEGTVSFLP 333



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>NAS38_CAEEL (Q20942) Zinc metalloproteinase nas-38 precursor (EC 3.4.24.21)|
           (Nematode astacin 38)
          Length = 745

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 26/84 (30%), Positives = 38/84 (45%), Gaps = 2/84 (2%)
 Frame = +2

Query: 29  QRANTTTVQSST*WGQA-QRRT*KVRGTMSSQ*SKRALRSTAGVSTLRMTTVALRRNSSR 205
           Q+   TTVQS+T +  A  RRT K +       +      T+  +T   TTV+    S+ 
Sbjct: 513 QKPEPTTVQSTTTYTTAIPRRTAKKQFFTRKPITIPLTPLTSSSTTTESTTVSSTTQSTT 572

Query: 206 YAPQEPQHYC-QTCSDPTSPTLVL 274
           + P EP     +T     SPT+ L
Sbjct: 573 WLPTEPSFATGETEITTASPTITL 596



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>PCX1_HUMAN (Q96RV3) Pecanex-like protein 1 (Pecanex homolog)|
          Length = 2341

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = +2

Query: 143 STAGVSTLRMTTVALRRNSSRYAPQEPQHYCQTCSDPTSPTLVL*SPAAQQ 295
           + +G +      V+ RR  S +  Q  +      S+PT PTL++ SP + Q
Sbjct: 767 AVSGAAQASEEAVSFRRERSTFRRQAVRRRHNAGSNPTPPTLLIGSPLSLQ 817



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>PGH1_RAT (Q63921) Prostaglandin G/H synthase 1 precursor (EC 1.14.99.1)|
           (Cyclooxygenase-1) (COX-1) (Prostaglandin-endoperoxide
           synthase 1) (Prostaglandin H2 synthase 1) (PGH synthase
           1) (PGHS-1) (PHS 1)
          Length = 602

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 13/24 (54%), Positives = 14/24 (58%)
 Frame = -1

Query: 239 SGNNAVVPEEHTWSCFSLRPQWSF 168
           SG N  +PE  TW   SLRP  SF
Sbjct: 67  SGPNCTIPEIWTWLRSSLRPSPSF 90



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>T2K1_KLEPN (P25237) Type II restriction enzyme KpnI (EC 3.1.21.4)|
           (Endonuclease KpnI) (R.KpnI)
          Length = 218

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 12/33 (36%), Positives = 15/33 (45%)
 Frame = +1

Query: 175 HCGLKEKQLQVCSSGTTALLPDMF*SYITDPGS 273
           HCGL E  +     GT  L PD    +  DP +
Sbjct: 127 HCGLVEGDIDPIGGGTVKLTPDHMTPHSIDPAT 159


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,386,119
Number of Sequences: 219361
Number of extensions: 880296
Number of successful extensions: 2431
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2370
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2430
length of database: 80,573,946
effective HSP length: 89
effective length of database: 61,050,817
effective search space used: 1465219608
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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