ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl18f02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TOP2_CANAL (P87078) DNA topoisomerase 2 (EC 5.99.1.3) (DNA topoi... 33 0.22
2LGRD_BREPA (Q70LM4) Linear gramicidin synthetase subunit D [Incl... 29 2.4
3PRIA_CHLTR (O84783) Primosomal protein N' (EC 3.6.1.-) (ATP-depe... 28 5.4

>TOP2_CANAL (P87078) DNA topoisomerase 2 (EC 5.99.1.3) (DNA topoisomerase II)|
          Length = 1461

 Score = 32.7 bits (73), Expect = 0.22
 Identities = 26/98 (26%), Positives = 41/98 (41%), Gaps = 1/98 (1%)
 Frame = -1

Query: 327  DRMRNNFVFFFIHSA*ETGSISNTE*AKKMWRRVM-TSNKQQGGREEAAVEEN*VQRDHT 151
            D     F  F +    E  S+++    K   RR   T+ K Q   ++  VE         
Sbjct: 1227 DEFLAEFDKFLLRDEQERESLASNGKKKSTKRRAKATATKDQPNNKKVKVEPK------- 1279

Query: 150  ERKNARLHTLIDYDLDNEVDACALPCPKEKQNALSFYS 37
            E+K+     ++  +  NE  A +   PKEK + LSF+S
Sbjct: 1280 EKKSTSAKPIVKKEASNEPQASSSSKPKEKDDILSFFS 1317



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>LGRD_BREPA (Q70LM4) Linear gramicidin synthetase subunit D [Includes:|
            ATP-dependent tryptophan adenylase (TrpA) (Tryptophan
            activase); ATP-dependent D-leucine adenylase (D-LeuA)
            (D-Leucine activase); Leucine racemase [ATP-hydrolyzing]
            (EC 5.1.1.-); ATP-d
          Length = 5085

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 27/100 (27%), Positives = 43/100 (43%), Gaps = 7/100 (7%)
 Frame = +2

Query: 68   FGHGRAHASTSLSRS*SINVCSRAFFRSVWSLWT*FSSTAASSLPPCCLLLVMTRLHIFF 247
            F H RA+  T+  R+    +   AF  SVW +W   +  A   LP   + LV  +L  + 
Sbjct: 1690 FWHQRAYDVTATDRA--SQIAGTAFDASVWEIWPYVTKGATLYLPEEEIRLVPEKLRDWL 1747

Query: 248  AYSVFEID----PVSH---ALWMKKNTKLFLMRSAGRRIH 346
              S   +     P++    AL    +T L  M + G ++H
Sbjct: 1748 VASNITVSFLPTPLTESMLALEWPGDTALRYMLTGGDKLH 1787



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>PRIA_CHLTR (O84783) Primosomal protein N' (EC 3.6.1.-) (ATP-dependent helicase|
           priA) (Replication factor Y)
          Length = 753

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = -1

Query: 159 DHTERKNARLHTLIDYDLDNEVDACAL-PCPKEKQNALSFYSLL 31
           D+T ++  R+HTLI  +LD++     + PC   K   L  Y  L
Sbjct: 671 DYTLKETQRVHTLIKQNLDSQASLMEISPCGHFKVKDLFHYQFL 714


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,371,162
Number of Sequences: 219361
Number of extensions: 937580
Number of successful extensions: 2272
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 2244
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2272
length of database: 80,573,946
effective HSP length: 100
effective length of database: 58,637,846
effective search space used: 1407308304
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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