ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl17h04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1YCFI_YEAST (P39109) Metal resistance protein YCF1 (Yeast cadmium... 31 0.66
2VPS34_SCHPO (P50520) Phosphatidylinositol 3-kinase vps34 (EC 2.7... 29 2.5
3DDX54_HUMAN (Q8TDD1) ATP-dependent RNA helicase DDX54 (EC 3.6.1.... 29 3.3
4GPRK2_DROME (P32866) G protein-coupled receptor kinase 2 (EC 2.7... 28 4.3
5FTSY_NEIMA (P57010) Cell division protein ftsY homolog 28 5.6
6FTSY_NEIMC (O30391) Cell division protein ftsY homolog 28 7.3
7FTSY_NEIMB (P57011) Cell division protein ftsY homolog 28 7.3
8BUB1B_HUMAN (O60566) Mitotic checkpoint serine/threonine-protein... 28 7.3
9RB3GP_BRARE (Q6NUV0) Rab3 GTPase-activating protein catalytic su... 28 7.3
10NU5M_PICCA (Q37024) NADH-ubiquinone oxidoreductase chain 5 (EC 1... 27 9.5
11FTSY_NEIGO (P14929) Cell division protein ftsY homolog 27 9.5
12MYSA_DROME (P05661) Myosin heavy chain, muscle 27 9.5

>YCFI_YEAST (P39109) Metal resistance protein YCF1 (Yeast cadmium factor 1)|
          Length = 1515

 Score = 31.2 bits (69), Expect = 0.66
 Identities = 24/80 (30%), Positives = 38/80 (47%), Gaps = 1/80 (1%)
 Frame = +3

Query: 6   YVASSLL-LHYIHSFIYNNTQNVMYSTTSHDAKCSTSCHKIYSSVSCLFGWL*IVWSQIH 182
           Y+ S+L  L Y  S + +N  + + ST       S    K+      L  WL ++WS   
Sbjct: 376 YIKSALTALIYQKSLVLSNEASGLSSTGDIVNLMSVDVQKLQD----LTQWLNLIWS--- 428

Query: 183 KA*KAPYQQLVCLESVHKLL 242
                P+Q ++CL S++KLL
Sbjct: 429 ----GPFQIIICLYSLYKLL 444



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>VPS34_SCHPO (P50520) Phosphatidylinositol 3-kinase vps34 (EC 2.7.1.137)|
           (PtdIns-3-kinase vps34) (PI3-kinase vps34) (PI3K vps34)
           (Vacuolar sorting protein 34)
          Length = 801

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 6/51 (11%)
 Frame = -1

Query: 191 SCLVYLRPDNL*PAKETRNRGI------NFVAACATFCVM*CCRVHYILGV 57
           S L YLR +N      + N GI      N+V +CA +CV     + Y+LGV
Sbjct: 610 SVLAYLRENNPDDGLNSANYGIDPVAMDNYVRSCAGYCV-----ITYLLGV 655



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>DDX54_HUMAN (Q8TDD1) ATP-dependent RNA helicase DDX54 (EC 3.6.1.-) (DEAD box|
           protein 54) (ATP-dependent RNA helicase DP97)
          Length = 881

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 8/49 (16%)
 Frame = +3

Query: 15  SSLLLHYIHSFIYNNTQNVMYSTTSHDAKCST--------SCHKIYSSV 137
           +++LLH +H+ +    Q V++  T H A+  T        SC  IYS++
Sbjct: 324 AAVLLHLLHNVVRPQDQTVVFVATKHHAEYLTELLTTQRVSCAHIYSAL 372



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>GPRK2_DROME (P32866) G protein-coupled receptor kinase 2 (EC 2.7.11.16)|
          Length = 714

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 20/77 (25%), Positives = 36/77 (46%), Gaps = 15/77 (19%)
 Frame = -3

Query: 333 GKEAVIRSEGVGFKRRGQVEEGSGG--------------LQRRPGEVCAQIQDRL-AAGR 199
           G E+V   EG     +G   EG GG              L     ++ AQ++++L + G+
Sbjct: 203 GDESVKCQEGHDDAEKGGGGEGGGGGKCVHVGGYPDELVLDVLNDDLIAQVRNKLNSGGK 262

Query: 198 ELFMPCVSETRQFIASQ 148
           ++F  CV+  + F+A +
Sbjct: 263 DIFAQCVNAVKAFLAGE 279



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>FTSY_NEIMA (P57010) Cell division protein ftsY homolog|
          Length = 421

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 16/67 (23%), Positives = 28/67 (41%)
 Frame = -3

Query: 327 EAVIRSEGVGFKRRGQVEEGSGGLQRRPGEVCAQIQDRLAAGRELFMPCVSETRQFIASQ 148
           E +  +E    K   QVE     +    GE   Q+Q+ +A   E  +   +  +Q +   
Sbjct: 74  EMLSEAEEAAEKAAEQVEAAKEAIAETVGEAVGQVQEAVATTEEHKLGWAARLKQGLTKS 133

Query: 147 RDKKPRN 127
           RDK  ++
Sbjct: 134 RDKMAKS 140



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>FTSY_NEIMC (O30391) Cell division protein ftsY homolog|
          Length = 421

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 16/67 (23%), Positives = 28/67 (41%)
 Frame = -3

Query: 327 EAVIRSEGVGFKRRGQVEEGSGGLQRRPGEVCAQIQDRLAAGRELFMPCVSETRQFIASQ 148
           E +  +E    K   QVE     +    GE   Q+Q+ +A   E  +   +  +Q +   
Sbjct: 74  EMLSEAEEAAEKAAEQVEAAKEAVAETVGEAVGQVQEAVATTEEHKLGWAARLKQGLTKS 133

Query: 147 RDKKPRN 127
           RDK  ++
Sbjct: 134 RDKMAKS 140



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>FTSY_NEIMB (P57011) Cell division protein ftsY homolog|
          Length = 421

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 16/67 (23%), Positives = 28/67 (41%)
 Frame = -3

Query: 327 EAVIRSEGVGFKRRGQVEEGSGGLQRRPGEVCAQIQDRLAAGRELFMPCVSETRQFIASQ 148
           E +  +E    K   QVE     +    GE   Q+Q+ +A   E  +   +  +Q +   
Sbjct: 74  EMLSEAEEAAEKAAEQVEAAKEAVAETVGEAVGQVQEAVATTEEHKLGWAARLKQGLTKS 133

Query: 147 RDKKPRN 127
           RDK  ++
Sbjct: 134 RDKMAKS 140



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>BUB1B_HUMAN (O60566) Mitotic checkpoint serine/threonine-protein kinase BUB1|
           beta (EC 2.7.11.1) (hBUBR1) (MAD3/BUB1-related protein
           kinase) (Mitotic checkpoint kinase MAD3L) (SSK1)
          Length = 1050

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 15/65 (23%), Positives = 32/65 (49%)
 Frame = -3

Query: 294 KRRGQVEEGSGGLQRRPGEVCAQIQDRLAAGRELFMPCVSETRQFIASQRDKKPRNKFCG 115
           ++R ++++    ++++  E+    Q+R    +E  MP    T+  IAS+  K P      
Sbjct: 437 EKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSS 496

Query: 114 SLCYI 100
           S+C +
Sbjct: 497 SVCQV 501



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>RB3GP_BRARE (Q6NUV0) Rab3 GTPase-activating protein catalytic subunit|
          Length = 969

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 16/63 (25%), Positives = 29/63 (46%)
 Frame = -3

Query: 312 SEGVGFKRRGQVEEGSGGLQRRPGEVCAQIQDRLAAGRELFMPCVSETRQFIASQRDKKP 133
           S+G   + RG   EG+G     P E   +  D  +   + F  CVS+T +    + + + 
Sbjct: 540 SDGARDRSRG-APEGAGPEGAGPAEAAGKSWDSWSDSEDEFFECVSDTEEMKEDKEEAEN 598

Query: 132 RNK 124
           R++
Sbjct: 599 RSR 601



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>NU5M_PICCA (Q37024) NADH-ubiquinone oxidoreductase chain 5 (EC 1.6.5.3)|
          Length = 644

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = +2

Query: 35  YSFIHLQQHPKCNVLYNIT*RKM*HKLPQNLFLGFLSLWLAINCLV 172
           YSFI++ Q  K   +YN       + LP N    F   W+AI C++
Sbjct: 431 YSFINIPQLNK--YIYNSLSLNNTYSLPFNFKFNFDKSWMAIICMI 474



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>FTSY_NEIGO (P14929) Cell division protein ftsY homolog|
          Length = 416

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 16/68 (23%), Positives = 28/68 (41%)
 Frame = -3

Query: 330 KEAVIRSEGVGFKRRGQVEEGSGGLQRRPGEVCAQIQDRLAAGRELFMPCVSETRQFIAS 151
           KE V        +   +VE     +    GE   Q+Q+ +A   E  +   +  +Q +A 
Sbjct: 69  KETVAEMPSEAGEAAERVESAKEAVAETVGEAVGQVQEAVATTEEHKLGWAARLKQGLAK 128

Query: 150 QRDKKPRN 127
            RDK  ++
Sbjct: 129 SRDKMAKS 136



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>MYSA_DROME (P05661) Myosin heavy chain, muscle|
          Length = 1962

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 18/74 (24%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
 Frame = -3

Query: 324 AVIRSEGVGFKRRGQVEEGSGGLQRRPGEVCAQIQ-DRLAAGRELFMPCVSETRQFIASQ 148
           AV+   G+ FK+RG+ E+     +   G V      D     + L  P +    +F+   
Sbjct: 354 AVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQG 413

Query: 147 RDKKPRNKFCGSLC 106
           R+ +      G+LC
Sbjct: 414 RNVQQVTNSIGALC 427


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,790,435
Number of Sequences: 219361
Number of extensions: 886562
Number of successful extensions: 2049
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2023
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2049
length of database: 80,573,946
effective HSP length: 93
effective length of database: 60,173,373
effective search space used: 1444160952
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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