ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl16c01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MA2B1_HUMAN (O00754) Lysosomal alpha-mannosidase precursor (EC 3... 63 3e-10
2MA2B1_MACFA (Q60HE9) Lysosomal alpha-mannosidase precursor (EC 3... 63 4e-10
3MA2B1_FELCA (O46432) Lysosomal alpha-mannosidase precursor (EC 3... 62 5e-10
4MA2B1_CAVPO (Q8VHC8) Lysosomal alpha-mannosidase precursor (EC 3... 62 9e-10
5MA2B1_BOVIN (Q29451) Lysosomal alpha-mannosidase precursor (EC 3... 62 9e-10
6MA2B1_MOUSE (O09159) Lysosomal alpha-mannosidase precursor (EC 3... 57 2e-08
7MA2B2_MOUSE (O54782) Epididymis-specific alpha-mannosidase precu... 46 4e-05
8MA2B2_PONPY (Q5RDJ3) Epididymis-specific alpha-mannosidase precu... 40 0.003
9MA2B2_HUMAN (Q9Y2E5) Epididymis-specific alpha-mannosidase precu... 40 0.004
10COMD1_HUMAN (Q8N668) COMM domain-containing protein 1 (Protein M... 31 1.3
11MA2B2_PIG (Q28949) Epididymis-specific alpha-mannosidase precurs... 30 2.3
12COMD1_CANFA (Q8WMD0) COMM domain-containing protein 1 (Protein M... 30 2.3
13VPS1_YEAST (P21576) Vacuolar sorting protein 1 30 3.0
14IDI_PHOPR (Q6LUX5) Isopentenyl-diphosphate delta-isomerase (EC 5... 30 3.9
15YKYB_BACSU (P42430) Hypothetical protein ykyB 30 3.9
16COMD1_MOUSE (Q8K4M5) COMM domain-containing protein 1 (Protein M... 28 8.7

>MA2B1_HUMAN (O00754) Lysosomal alpha-mannosidase precursor (EC 3.2.1.24)|
            (Mannosidase, alpha B) (Lysosomal acid alpha-mannosidase)
            (Laman) (Mannosidase alpha class 2B member 1) [Contains:
            Lysosomal alpha-mannosidase A peptide; Lysosomal
            alpha-mannosidase
          Length = 1010

 Score = 63.2 bits (152), Expect = 3e-10
 Identities = 43/114 (37%), Positives = 54/114 (47%), Gaps = 2/114 (1%)
 Frame = -2

Query: 433  LPDNVAIITLQNLDDGTTLLRLGHLFQAAEDPQYSVMAKV--ELKKLFGKRTIKELTETN 260
            LP +V ++TL +      LLRL H F   ED   ++ A V   L+ LF   TI  L ET 
Sbjct: 895  LPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETT 954

Query: 259  LSANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLVKL 98
            L ANQ + A  +LKW           P    P N     + L PMEIRTFL  +
Sbjct: 955  LVANQLREAASRLKWTTNTGPTPHQTPYQLDPAN-----ITLEPMEIRTFLASV 1003



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>MA2B1_MACFA (Q60HE9) Lysosomal alpha-mannosidase precursor (EC 3.2.1.24)|
            (Mannosidase, alpha B) (Lysosomal acid alpha-mannosidase)
            (Laman) (Mannosidase alpha class 2B member 1)
          Length = 1012

 Score = 62.8 bits (151), Expect = 4e-10
 Identities = 43/114 (37%), Positives = 54/114 (47%), Gaps = 2/114 (1%)
 Frame = -2

Query: 433  LPDNVAIITLQNLDDGTTLLRLGHLFQAAEDPQYSVMAKV--ELKKLFGKRTIKELTETN 260
            LP +V ++TL +      LLRL H F   ED   ++ A V   L+ LF   TI  L ET 
Sbjct: 897  LPPSVHLLTLASWGPEMLLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETT 956

Query: 259  LSANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLVKL 98
            L ANQ + A  +LKW           P    P N     + L PMEIRTFL  +
Sbjct: 957  LVANQLREAASRLKWTTNTGPTPHQTPYQLDPAN-----ITLEPMEIRTFLASV 1005



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>MA2B1_FELCA (O46432) Lysosomal alpha-mannosidase precursor (EC 3.2.1.24)|
            (Mannosidase, alpha B) (Lysosomal acid alpha-mannosidase)
            (Laman) (Mannosidase alpha class 2B member 1)
          Length = 1007

 Score = 62.4 bits (150), Expect = 5e-10
 Identities = 43/113 (38%), Positives = 56/113 (49%), Gaps = 1/113 (0%)
 Frame = -2

Query: 433  LPDNVAIITLQNLDDGTTLLRLGHLFQAAEDP-QYSVMAKVELKKLFGKRTIKELTETNL 257
            LP +V ++TL   D  T LLRL H F   ED    S    ++L  LF   TI  L ET L
Sbjct: 894  LPPSVHLLTLARWDQKTLLLRLEHQFAVGEDSGNLSSPVTLDLTDLFSAFTITYLQETTL 953

Query: 256  SANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLVKL 98
             ANQ +++  +LKW     T ++      SP       + L PMEIRTFL  +
Sbjct: 954  VANQLRASASRLKW-----TPNTGPTPLPSPSRLDPATITLQPMEIRTFLASV 1001



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>MA2B1_CAVPO (Q8VHC8) Lysosomal alpha-mannosidase precursor (EC 3.2.1.24)|
            (Mannosidase, alpha B) (Lysosomal acid alpha-mannosidase)
            (Laman) (Mannosidase alpha class 2B member 1)
          Length = 1007

 Score = 61.6 bits (148), Expect = 9e-10
 Identities = 42/114 (36%), Positives = 58/114 (50%), Gaps = 2/114 (1%)
 Frame = -2

Query: 433  LPDNVAIITLQNLDDGTTLLRLGHLFQAAEDPQ--YSVMAKVELKKLFGKRTIKELTETN 260
            LP +V ++TL      T LLRL H F   ED     S+   ++L+ LF   TI  L ET 
Sbjct: 893  LPPSVRLLTLARWGPDTLLLRLEHQFALGEDSSRNLSLPVTLDLQDLFSTFTITRLQETT 952

Query: 259  LSANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLVKL 98
            L+ANQ +++  +LKW    D  S  A     P +     + L PMEIRTF+  +
Sbjct: 953  LAANQLRASASRLKWTTEIDPISRPAVPRLDPSS-----ITLQPMEIRTFVASV 1001



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>MA2B1_BOVIN (Q29451) Lysosomal alpha-mannosidase precursor (EC 3.2.1.24)|
            (Mannosidase, alpha B) (Lysosomal acid alpha-mannosidase)
            (Laman) (Mannosidase alpha class 2B member 1) [Contains:
            Lysosomal alpha-mannosidase A peptide; Lysosomal
            alpha-mannosidase
          Length = 999

 Score = 61.6 bits (148), Expect = 9e-10
 Identities = 43/114 (37%), Positives = 58/114 (50%), Gaps = 2/114 (1%)
 Frame = -2

Query: 433  LPDNVAIITLQNLDDGTTLLRLGHLFQAAEDPQYSVMAKV--ELKKLFGKRTIKELTETN 260
            LP +V ++TL      T LLRL H F   ED   ++ + V  +L  LF   TI  L ET 
Sbjct: 885  LPPSVRLLTLARWGPETLLLRLEHQFAVGEDSGRNLSSPVTLDLTNLFSAFTITNLRETT 944

Query: 259  LSANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLVKL 98
            L+ANQ  +   +L+W    DT  +  P    PV+     + L PMEIRTFL  +
Sbjct: 945  LAANQLLAYASRLQWTT--DTGPTPHPSPSRPVS---ATITLQPMEIRTFLASV 993



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>MA2B1_MOUSE (O09159) Lysosomal alpha-mannosidase precursor (EC 3.2.1.24)|
            (Mannosidase, alpha B) (Lysosomal acid alpha-mannosidase)
            (Laman) (Mannosidase alpha class 2B member 1)
          Length = 1013

 Score = 57.4 bits (137), Expect = 2e-08
 Identities = 43/114 (37%), Positives = 53/114 (46%), Gaps = 2/114 (1%)
 Frame = -2

Query: 433  LPDNVAIITLQNLDDGTTLLRLGHLFQAAEDPQYSVMAKVEL--KKLFGKRTIKELTETN 260
            LP  V ++TL        LLRL H F   ED   ++ + V L  + LF   TI  L ET 
Sbjct: 897  LPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETT 956

Query: 259  LSANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLVKL 98
            L+ANQ  S   +LKW       S   P    P +     V L PMEIRTFL  +
Sbjct: 957  LAANQPLSRASRLKWMTNTGPTSFPEPSKLDPTS-----VTLKPMEIRTFLASV 1005



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>MA2B2_MOUSE (O54782) Epididymis-specific alpha-mannosidase precursor (EC|
            3.2.1.24) (Mannosidase alpha class 2B member 2)
          Length = 1018

 Score = 46.2 bits (108), Expect = 4e-05
 Identities = 31/94 (32%), Positives = 45/94 (47%), Gaps = 1/94 (1%)
 Frame = -2

Query: 379  LLRLGHLFQAAEDPQYSVMAKVELK-KLFGKRTIKELTETNLSANQKKSAMRKLKWRVVG 203
            LLRL HL++A EDP  S  A V+LK  L G  ++  + E +L+       +++  W    
Sbjct: 924  LLRLRHLYEAGEDPVLSRPATVDLKVVLRGLGSVVAVEERSLTGTWDVQMLQRWHWSTKT 983

Query: 202  DTESSHAPVTGSPVNDKVLVVELGPMEIRTFLVK 101
            D    H      P    ++ V   P EIRTF +K
Sbjct: 984  DHLKGHPTSPPRPPGGSIITVY--PKEIRTFFIK 1015



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>MA2B2_PONPY (Q5RDJ3) Epididymis-specific alpha-mannosidase precursor (EC|
            3.2.1.24) (Mannosidase alpha class 2B member 2)
          Length = 1009

 Score = 40.0 bits (92), Expect = 0.003
 Identities = 30/98 (30%), Positives = 44/98 (44%), Gaps = 6/98 (6%)
 Frame = -2

Query: 379  LLRLGHLFQAAEDPQYSVMAKVELKK-LFGKRTIKELTETNLSANQKKSAMRKLKWRV-- 209
            LLRL HL++  EDP  S    V L+  L    ++  + E +L+     S + +  WR   
Sbjct: 914  LLRLHHLYEVGEDPVLSQPVTVNLEAVLQALGSVVAVEERSLTGTWDVSMLHRWSWRTGS 973

Query: 208  ---VGDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLV 104
                GDT S   P  G        ++ + P EIRTF +
Sbjct: 974  GRRRGDTTSPSRPPGGP-------IITVHPKEIRTFFI 1004



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>MA2B2_HUMAN (Q9Y2E5) Epididymis-specific alpha-mannosidase precursor (EC|
            3.2.1.24) (Mannosidase alpha class 2B member 2)
          Length = 1009

 Score = 39.7 bits (91), Expect = 0.004
 Identities = 30/98 (30%), Positives = 44/98 (44%), Gaps = 6/98 (6%)
 Frame = -2

Query: 379  LLRLGHLFQAAEDPQYSVMAKVELKK-LFGKRTIKELTETNLSANQKKSAMRKLKWRV-- 209
            LLRL HL++  EDP  S    V L+  L    ++  + E +L+     S + +  WR   
Sbjct: 914  LLRLYHLYEVGEDPVLSQPVTVNLQAVLQALGSVVAVEERSLTGTWDLSMLHRWSWRTGP 973

Query: 208  ---VGDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLV 104
                GDT S   P  G        ++ + P EIRTF +
Sbjct: 974  GRHRGDTTSPSRPPGGP-------IITVHPKEIRTFFI 1004



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>COMD1_HUMAN (Q8N668) COMM domain-containing protein 1 (Protein Murr1)|
          Length = 190

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 17/56 (30%), Positives = 32/56 (57%)
 Frame = -2

Query: 298 FGKRTIKELTETNLSANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELG 131
           F K    ++ E+ ++ ++  S +R L WRV G ++S H+    +PV   ++ +ELG
Sbjct: 97  FWKSHKTKIRESLMNQSRWNSGLRGLSWRVDGKSQSRHSAQIHTPV--AIIELELG 150



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>MA2B2_PIG (Q28949) Epididymis-specific alpha-mannosidase precursor (EC|
            3.2.1.24) (Mannosidase alpha class 2B member 2) (AMAN)
          Length = 995

 Score = 30.4 bits (67), Expect = 2.3
 Identities = 25/99 (25%), Positives = 43/99 (43%), Gaps = 7/99 (7%)
 Frame = -2

Query: 379  LLRLGHLFQAAEDPQYSVMAKVELKK-LFGKRTIKELTETNLSANQKKSAMRKLKWRVV- 206
            LLRL HL++A E    S    + L+  L G  ++  + E +L+     +++ +  W+   
Sbjct: 899  LLRLHHLYEAGEHQALSRPVTLNLQSVLRGLGSVVAVEERSLTGTWDVNSLHRWSWKTED 958

Query: 205  -----GDTESSHAPVTGSPVNDKVLVVELGPMEIRTFLV 104
                 G +     P+ G  V        + P EIRTF +
Sbjct: 959  GHHHRGSSRRPLPPLRGPNVT-------IHPKEIRTFFI 990



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>COMD1_CANFA (Q8WMD0) COMM domain-containing protein 1 (Protein Murr1)|
          Length = 188

 Score = 30.4 bits (67), Expect = 2.3
 Identities = 16/56 (28%), Positives = 32/56 (57%)
 Frame = -2

Query: 298 FGKRTIKELTETNLSANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELG 131
           F K    ++ E+ ++ ++  S +R L WRV G ++S H+    +PV   ++ +E+G
Sbjct: 96  FWKNHKTKIRESLMNQSRWDSGLRGLSWRVDGKSQSRHSAQIHTPV--AIMELEIG 149



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>VPS1_YEAST (P21576) Vacuolar sorting protein 1|
          Length = 704

 Score = 30.0 bits (66), Expect = 3.0
 Identities = 14/33 (42%), Positives = 23/33 (69%)
 Frame = -2

Query: 319 KVELKKLFGKRTIKELTETNLSANQKKSAMRKL 221
           KV L+KL+GK+ I+ELT+ N    Q++   +K+
Sbjct: 658 KVLLEKLYGKQDIEELTKENDITIQRRKECKKM 690



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>IDI_PHOPR (Q6LUX5) Isopentenyl-diphosphate delta-isomerase (EC 5.3.3.2) (IPP|
           isomerase) (Isopentenyl pyrophosphate isomerase)
           (IPP:DMAPP isomerase)
          Length = 180

 Score = 29.6 bits (65), Expect = 3.9
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = +1

Query: 13  AFAHRNERHNNLLEHAWNYITISLLLQLTVSP 108
           +F +R E  N L+EH W+++ I+   +L + P
Sbjct: 104 SFVYRAELDNQLIEHEWDHVLIANGTELAIIP 135



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>YKYB_BACSU (P42430) Hypothetical protein ykyB|
          Length = 154

 Score = 29.6 bits (65), Expect = 3.9
 Identities = 13/24 (54%), Positives = 15/24 (62%)
 Frame = +1

Query: 124 PWVPARQQGLYH*PGYR*LGRDYF 195
           P VP RQQ  YH P ++ LG  YF
Sbjct: 131 PLVPNRQQPAYHKPVFKKLGESYF 154



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>COMD1_MOUSE (Q8K4M5) COMM domain-containing protein 1 (Protein Murr1)|
          Length = 188

 Score = 28.5 bits (62), Expect = 8.7
 Identities = 17/60 (28%), Positives = 32/60 (53%)
 Frame = -2

Query: 310 LKKLFGKRTIKELTETNLSANQKKSAMRKLKWRVVGDTESSHAPVTGSPVNDKVLVVELG 131
           + K +    IK + E+ +  ++  + +R L WRV G ++S H+    SPV   ++ +E G
Sbjct: 93  ISKFWKSHKIK-IRESLMKQSRWDNGLRGLSWRVDGKSQSRHSTQIHSPV--AIIELEFG 149


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,315,985
Number of Sequences: 219361
Number of extensions: 1197159
Number of successful extensions: 3256
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 3123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3243
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2968155324
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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