ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl16a08
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RK4_ODOSI (P49546) Chloroplast 50S ribosomal protein L4 30 1.4
2CS120_WHEAT (P46525) Cold-shock protein CS120 29 3.2
3ENGC_PSEPK (Q88DC4) Probable GTPase engC (EC 3.6.1.-) 28 4.2
4TSH1_MOUSE (Q5DTH5) Teashirt homolog 1 (Serologically defined co... 28 5.5
5RRMJ2_DROME (Q9VDD9) Putative ribosomal RNA methyltransferase CG... 28 7.2
6GUND_CELFI (P50400) Endoglucanase D precursor (EC 3.2.1.4) (Endo... 27 9.3
7COG2_YEAST (P53271) Conserved oligomeric Golgi complex component... 27 9.3
8RPOA_PRRSB (Q8B912) Replicase polyprotein 1ab (ORF1ab polyprotei... 27 9.3
9RPOA_PRRSL (Q04561) Replicase polyprotein 1ab (ORF1ab polyprotei... 27 9.3

>RK4_ODOSI (P49546) Chloroplast 50S ribosomal protein L4|
          Length = 215

 Score = 30.0 bits (66), Expect = 1.4
 Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
 Frame = +1

Query: 142 NSADTTSPTATTGLRSIAHSIQKSGSYL-HKVKNGHQDHQESGQISLTTATQ 294
           NS D    T +   +   + ++KSG+YL HK    HQ  Q  G IS  T ++
Sbjct: 10  NSVDINGKTLSDEYKLELNVLEKSGNYLIHKDILRHQSSQRQGTISTKTRSE 61



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>CS120_WHEAT (P46525) Cold-shock protein CS120|
          Length = 391

 Score = 28.9 bits (63), Expect = 3.2
 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
 Frame = +1

Query: 166 TATTGLRSIAHSIQKSG---SYLHKVKNGHQDHQESG 267
           T  TGL   AH+ +K G   +   K+  GHQDHQ++G
Sbjct: 61  TTGTGLHG-AHAGEKKGVMENIKDKLPGGHQDHQQTG 96



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>ENGC_PSEPK (Q88DC4) Probable GTPase engC (EC 3.6.1.-)|
          Length = 343

 Score = 28.5 bits (62), Expect = 4.2
 Identities = 24/68 (35%), Positives = 33/68 (48%), Gaps = 15/68 (22%)
 Frame = -3

Query: 334 ADLVN*ESLG*PH--------IGSPWLEKSVHFPDG-------LDAHFSLYGGMNQTSGW 200
           ADL+N E+    H        +G P LE S H  DG       LD H S++ G +   G 
Sbjct: 165 ADLINDENGPGLHALLEVYRELGYPLLEVSAHHGDGMQRLQQQLDGHISVFVGQSGV-GK 223

Query: 199 SELLNAVL 176
           S L+N++L
Sbjct: 224 SSLVNSLL 231



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>TSH1_MOUSE (Q5DTH5) Teashirt homolog 1 (Serologically defined colon cancer|
           antigen 3 homolog)
          Length = 1084

 Score = 28.1 bits (61), Expect = 5.5
 Identities = 15/57 (26%), Positives = 28/57 (49%)
 Frame = +1

Query: 139 SNSADTTSPTATTGLRSIAHSIQKSGSYLHKVKNGHQDHQESGQISLTTATQYAVSP 309
           +++A T   TATT + S + S   S +  +   +G+  HQ +   +L   + Y + P
Sbjct: 162 TSTASTACTTATTAITSCSTSTSHSSTTSNSSSSGYDWHQAALAKTLQQTSSYGLLP 218



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>RRMJ2_DROME (Q9VDD9) Putative ribosomal RNA methyltransferase CG7009 (EC|
           2.1.1.-) (rRNA (uridine-2'-O-)-methyltransferase)
          Length = 320

 Score = 27.7 bits (60), Expect = 7.2
 Identities = 15/43 (34%), Positives = 19/43 (44%)
 Frame = -2

Query: 284 VVREICPLS*WS*CPFFTLWRYEPDFWME*AIERSPVVAVGLV 156
           V RE C    +  C   T W  +P+ W+    E  PVV V  V
Sbjct: 201 VAREFCLPDGYKPCNLTTEWHDQPESWVGRKKESPPVVQVPFV 243



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>GUND_CELFI (P50400) Endoglucanase D precursor (EC 3.2.1.4)|
           (Endo-1,4-beta-glucanase) (Cellulase)
          Length = 747

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 7/59 (11%)
 Frame = +1

Query: 151 DTTSPTATTGLRS---IAHSIQKSGSYLHKVKN----GHQDHQESGQISLTTATQYAVS 306
           DTT+PTA TGLR+    A ++  + S           G++ ++ +  +  TTAT Y V+
Sbjct: 450 DTTAPTAPTGLRAGTPTASTVPLTWSASTDTGGSGVAGYEVYRGTTLVGTTTATSYTVT 508



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>COG2_YEAST (P53271) Conserved oligomeric Golgi complex component 2 (SEC35|
           protein)
          Length = 275

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
 Frame = +1

Query: 175 TGLRSIAHSIQKSGS-YLHKVKNGHQDHQESGQISLTTATQ 294
           T  R I  S   S S Y+H ++N +QD  +  QISL   T+
Sbjct: 212 TASRRILESCADSNSPYIHHLRNDYQDLLQEFQISLKILTE 252



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>RPOA_PRRSB (Q8B912) Replicase polyprotein 1ab (ORF1ab polyprotein) [Includes:|
            Replicase polyprotein 1a (ORF1a)] [Contains: Nsp1-alpha
            papain-like cysteine proteinase (EC 3.4.22.-)
            (PCP1-alpha); Nsp1-beta papain-like cysteine proteinase
            (EC 3.4.22.-) (PCP
          Length = 3963

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 14/36 (38%), Positives = 18/36 (50%)
 Frame = -2

Query: 245  CPFFTLWRYEPDFWME*AIERSPVVAVGLVVSAELL 138
            C FF LWR     W       +P+VAVG  +  E+L
Sbjct: 2023 CVFFLLWRMMGHAW-------TPLVAVGFFILNEIL 2051



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>RPOA_PRRSL (Q04561) Replicase polyprotein 1ab (ORF1ab polyprotein) [Includes:|
            Replicase polyprotein 1a (ORF1a)] [Contains: Nsp1-alpha
            papain-like cysteine proteinase (EC 3.4.22.-)
            (PCP1-alpha); Nsp1-beta papain-like cysteine proteinase
            (EC 3.4.22.-) (PCP
          Length = 3859

 Score = 27.3 bits (59), Expect = 9.3
 Identities = 14/36 (38%), Positives = 18/36 (50%)
 Frame = -2

Query: 245  CPFFTLWRYEPDFWME*AIERSPVVAVGLVVSAELL 138
            C FF LWR     W       +P+VAVG  +  E+L
Sbjct: 1906 CVFFLLWRMMGHAW-------TPIVAVGFFLLNEIL 1934


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,645,812
Number of Sequences: 219361
Number of extensions: 965275
Number of successful extensions: 2289
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 2236
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2289
length of database: 80,573,946
effective HSP length: 97
effective length of database: 59,295,929
effective search space used: 1423102296
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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