ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl14c03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1VD10_SFVKA (P32097) Protein D10 32 0.36
2TPP2_RAT (Q64560) Tripeptidyl-peptidase 2 (EC 3.4.14.10) (Tripep... 29 2.3
3TPP2_MOUSE (Q64514) Tripeptidyl-peptidase 2 (EC 3.4.14.10) (Trip... 29 2.3
4TPP2_HUMAN (P29144) Tripeptidyl-peptidase 2 (EC 3.4.14.10) (Trip... 29 2.3
5CDK7_MOUSE (Q03147) Cell division protein kinase 7 (EC 2.7.11.22... 29 3.1
6RGS6_CAEEL (Q18563) Regulator of G-protein signaling rgs-6 29 3.1
7AP1M_DISOM (P47795) AP-1 complex subunit mu (Clathrin coat assem... 28 4.0
8GLT1_SCHPO (Q9C102) Putative glutamate synthase [NADPH] (EC 1.4.... 28 5.2
9CVP4_PIMHY (Q8T0W2) Pacifastin-like protease inhibitor cvp4 prec... 28 5.2
10HUWE1_HUMAN (Q7Z6Z7) HECT, UBA and WWE domain-containing protein... 28 5.2
11INX10_CAEEL (Q22549) Innexin-10 28 6.8
12VL2_HPV31 (P17389) Minor capsid protein L2 27 8.9
13NUKM_BRAOL (P42027) NADH-ubiquinone oxidoreductase 20 kDa subuni... 27 8.9
14RPOA_SACOF (Q6ENT2) DNA-directed RNA polymerase alpha chain (EC ... 27 8.9
15RPOA_SACHY (Q6L370) DNA-directed RNA polymerase alpha chain (EC ... 27 8.9

>VD10_SFVKA (P32097) Protein D10|
          Length = 260

 Score = 32.0 bits (71), Expect = 0.36
 Identities = 20/68 (29%), Positives = 30/68 (44%)
 Frame = +2

Query: 56  TGMQNQAVDWSSKRLKKILEIATSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTK 235
           TG  NQ + WS+K     L ++T  N F+A S  H  L         +F+    F+T T+
Sbjct: 37  TGFSNQRLRWSNKT-SICLILSTLDNKFIACSRKHSFLYSEIVRCRSVFRKKRLFLTYTR 95

Query: 236 LPPSRPAP 259
               +  P
Sbjct: 96  FLKKKERP 103



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>TPP2_RAT (Q64560) Tripeptidyl-peptidase 2 (EC 3.4.14.10)|
            (Tripeptidyl-peptidase II) (TPP-II) (Tripeptidyl
            aminopeptidase)
          Length = 1248

 Score = 29.3 bits (64), Expect = 2.3
 Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +1

Query: 37   LVNKGHYRYAKPGSRLVEQK-IKKNTRNCYQFKYVFSTKQCTPGSTN 174
            LVNK + R  K  ++LVE+K  K+N +NC Q   +     C   + N
Sbjct: 1189 LVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTEN 1235



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>TPP2_MOUSE (Q64514) Tripeptidyl-peptidase 2 (EC 3.4.14.10)|
            (Tripeptidyl-peptidase II) (TPP-II) (Tripeptidyl
            aminopeptidase)
          Length = 1261

 Score = 29.3 bits (64), Expect = 2.3
 Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +1

Query: 37   LVNKGHYRYAKPGSRLVEQK-IKKNTRNCYQFKYVFSTKQCTPGSTN 174
            LVNK + R  K  ++LVE+K  K+N +NC Q   +     C   + N
Sbjct: 1202 LVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTEN 1248



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>TPP2_HUMAN (P29144) Tripeptidyl-peptidase 2 (EC 3.4.14.10)|
            (Tripeptidyl-peptidase II) (TPP-II) (Tripeptidyl
            aminopeptidase)
          Length = 1248

 Score = 29.3 bits (64), Expect = 2.3
 Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +1

Query: 37   LVNKGHYRYAKPGSRLVEQK-IKKNTRNCYQFKYVFSTKQCTPGSTN 174
            LVNK + R  K  ++LVE+K  K+N +NC Q   +     C   + N
Sbjct: 1189 LVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTEN 1235



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>CDK7_MOUSE (Q03147) Cell division protein kinase 7 (EC 2.7.11.22) (EC|
           2.7.11.23) (CDK-activating kinase) (CAK) (TFIIH basal
           transcription factor complex kinase subunit) (39 kDa
           protein kinase) (P39 Mo15) (Protein-tyrosine kinase
           MPK-7) (CR4 protein kinas
          Length = 346

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 15/36 (41%), Positives = 19/36 (52%), Gaps = 6/36 (16%)
 Frame = +2

Query: 191 EDLFKMNTCFITT------TKLPPSRPAPTPGMEMP 280
           + LF  N C  TT      TK   +RP PTPG ++P
Sbjct: 273 QGLFLFNPCTRTTASQALKTKYFSNRPGPTPGCQLP 308



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>RGS6_CAEEL (Q18563) Regulator of G-protein signaling rgs-6|
          Length = 737

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 17/46 (36%), Positives = 21/46 (45%)
 Frame = +1

Query: 196 FVQNEHMFHNHNQTPPKSTCAHARHGNASFIFGLFIRPQHNEQTLD 333
           F+     F N   TP K  C  ARH   S   G  +RP  ++ TLD
Sbjct: 329 FLPANFSFKNPASTPTKLVCIRARH---SLSTGAVLRPLLHKYTLD 371



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>AP1M_DISOM (P47795) AP-1 complex subunit mu (Clathrin coat assembly protein|
           AP47 homolog) (Clathrin coat-associated protein AP47
           homolog) (Golgi adaptor AP-1 47 kDa protein homolog)
           (HA1 47 kDa subunit homolog) (Clathrin assembly protein
           assembly protein
          Length = 418

 Score = 28.5 bits (62), Expect = 4.0
 Identities = 14/40 (35%), Positives = 21/40 (52%)
 Frame = -2

Query: 389 HTLFDLDGGMAVLLVPPWTSRVCSLCCGLMNKPKIKLAFP 270
           H+LF ++GG AV L   W S V    C  + + ++K   P
Sbjct: 3   HSLFLMNGGGAVFLEKHWRSVVSRSVCAYLLEAQLKAGQP 42



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>GLT1_SCHPO (Q9C102) Putative glutamate synthase [NADPH] (EC 1.4.1.13)|
            (NADPH-GOGAT)
          Length = 2111

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 19/51 (37%), Positives = 24/51 (47%), Gaps = 3/51 (5%)
 Frame = -2

Query: 380  FDLDGGMAVLL--VPPWTSRVCSLCCGLMNKPKIKL-AFPCLAWAQVDLGG 237
            FD D   A+ +  V PWT  V   C G M+   I + +   LA A   LGG
Sbjct: 920  FDFDSSQAIPIEQVEPWTEIVRRFCTGAMSYGSISMESHSSLAIAMNRLGG 970



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>CVP4_PIMHY (Q8T0W2) Pacifastin-like protease inhibitor cvp4 precursor|
           (Cysteine-rich venom protein 4)
          Length = 203

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 15/42 (35%), Positives = 21/42 (50%), Gaps = 3/42 (7%)
 Frame = +1

Query: 49  GHYRYAKPGSRLVEQKIKKNTRNC---YQFKYVFSTKQCTPG 165
           G  ++ KPG  + E++ KKN   C     FKY  +  QC  G
Sbjct: 65  GTRKFPKPGKWISEKENKKNDEPCTPGENFKYYCNDCQCLDG 106



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>HUWE1_HUMAN (Q7Z6Z7) HECT, UBA and WWE domain-containing protein 1 (EC 6.3.2.-)|
            (E3 ubiquitin protein ligase URE-B1) (Mcl-1 ubiquitin
            ligase E3) (Mule) (ARF-binding protein 1) (ARF-BP1)
          Length = 4374

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 22/65 (33%), Positives = 28/65 (43%), Gaps = 1/65 (1%)
 Frame = +2

Query: 95   RLKKILEIATSSNMFL-AQSNVHQALPI*STTTEDLFKMNTCFITTTKLPPSRPAPTPGM 271
            RL  ++ IA   N    AQ+N        +T T       T   +TT  PP+  APTP  
Sbjct: 3455 RLLSLISIALPENKVSEAQANSGSGASSTTTATSTTSTTTTTAASTTPTPPT--APTPVT 3512

Query: 272  EMPAL 286
              PAL
Sbjct: 3513 SAPAL 3517



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>INX10_CAEEL (Q22549) Innexin-10|
          Length = 559

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 15/56 (26%), Positives = 28/56 (50%)
 Frame = +1

Query: 100 KKNTRNCYQFKYVFSTKQCTPGSTNLIHHN*RFVQNEHMFHNHNQTPPKSTCAHAR 267
           K+N +N  Q  Y ++ +  TP      + N   +QN++ + N+ +TP  S    +R
Sbjct: 490 KQNQQNATQPPYCYTNQNPTP------YQNQNQIQNQNQYSNYYRTPSLSRGTDSR 539



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>VL2_HPV31 (P17389) Minor capsid protein L2|
          Length = 466

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 16/60 (26%), Positives = 28/60 (46%)
 Frame = +2

Query: 122 TSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTKLPPSRPAPTPGMEMPALFSVYS 301
           TS ++ L+ S++       ST   +   M+T  ++T     +   P PG+  PA   +YS
Sbjct: 172 TSGHLLLSSSSI-------STHNYEEIPMDTFIVSTNNENITSSTPIPGVRRPARLGLYS 224



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>NUKM_BRAOL (P42027) NADH-ubiquinone oxidoreductase 20 kDa subunit,|
           mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3)
           (Complex I-20KD) (CI-20KD)
          Length = 215

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 20/62 (32%), Positives = 27/62 (43%)
 Frame = +2

Query: 110 LEIATSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTKLPPSRPAPTPGMEMPALF 289
           L +   S+   A S++H +LP  S  T       T    T   PPS  AP PG+   A F
Sbjct: 12  LPLVLQSHRAAAVSHLHTSLPALSPAT-------TPTSYTRPGPPSTSAPPPGLSKTAEF 64

Query: 290 SV 295
            +
Sbjct: 65  VI 66



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>RPOA_SACOF (Q6ENT2) DNA-directed RNA polymerase alpha chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase alpha subunit) (RNA
           polymerase alpha subunit)
          Length = 339

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 14/43 (32%), Positives = 25/43 (58%)
 Frame = +2

Query: 110 LEIATSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTKL 238
           LE+ + +   L ++N+H  L + S T EDL ++N+  +   KL
Sbjct: 277 LELPSRTYNCLKRANIHTLLDLLSKTEEDLMRINSFRMEDGKL 319



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>RPOA_SACHY (Q6L370) DNA-directed RNA polymerase alpha chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase alpha subunit) (RNA
           polymerase alpha subunit)
          Length = 339

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 14/43 (32%), Positives = 25/43 (58%)
 Frame = +2

Query: 110 LEIATSSNMFLAQSNVHQALPI*STTTEDLFKMNTCFITTTKL 238
           LE+ + +   L ++N+H  L + S T EDL ++N+  +   KL
Sbjct: 277 LELPSRTYNCLKRANIHTLLDLLSKTEEDLMRINSFRMEDGKL 319


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,884,602
Number of Sequences: 219361
Number of extensions: 1355855
Number of successful extensions: 3609
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 3487
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3602
length of database: 80,573,946
effective HSP length: 110
effective length of database: 56,444,236
effective search space used: 1354661664
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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