Clone Information

BLAST Search Result

Clone Name	rbastl12f05
Clone Library Name	barley_pub

No.	Definition	Score (bits)	E Value
1	GAG_EIAVY (P69732) Gag polyprotein [Contains: Matrix protein p15...	30	1.5
2	GAG_EIAVC (P69731) Gag polyprotein [Contains: Matrix protein p15...	30	1.5
3	GAG_EIAV9 (P69730) Gag polyprotein [Contains: Matrix protein p15...	30	1.5
4	GAG_SIVV1 (P27972) Gag polyprotein [Contains: Core protein p17; ...	29	3.3
5	POL_HV2D2 (P15833) Gag-Pol polyprotein (Pr160Gag-Pol) [Contains:...	29	3.3
6	DHX15_MOUSE (O35286) Putative pre-mRNA-splicing factor ATP-depen...	29	3.3
7	DHX15_HUMAN (O43143) Putative pre-mRNA-splicing factor ATP-depen...	29	3.3
8	AIR2_YEAST (Q12476) Protein AIR2 (Arginine methyltransferase-int...	29	3.3
9	GAG_MMTVC (P11284) Gag polyprotein [Contains: Protein p10; Phosp...	28	4.3
10	POL_HV2G1 (P18042) Gag-Pol polyprotein (Pr160Gag-Pol) [Contains:...	28	5.7
11	GAG_SIVVT (P05892) Gag polyprotein [Contains: Core protein p17; ...	28	5.7
12	DEVR_MYXXA (Q07765) Fruiting body developmental protein R	28	5.7
13	GAG_MMTVB (P10258) Gag polyprotein [Contains: Protein p10; Phosp...	28	5.7
14	EXG_KLULA (Q12628) Glucan 1,3-beta-glucosidase precursor (EC 3.2...	28	7.4
15	GAG_SIVS4 (P12496) Gag polyprotein [Contains: Core protein p17; ...	28	7.4
16	NU2M_MYXGL (O21078) NADH-ubiquinone oxidoreductase chain 2 (EC 1...	28	7.4
17	YEW7_YEAST (P40083) Hypothetical 16.6 kDa protein in GDI1-COX15 ...	27	9.7
18	MAB3_CAEEL (O18214) Protein male abnormal 3	27	9.7
19	POLG_HCVED (O39929) Genome polyprotein [Contains: Core protein p...	27	9.7
20	AIR1_YEAST (P40507) Protein AIR1 (Arginine methyltransferase-int...	27	9.7

>GAG_EIAVY (P69732) Gag polyprotein [Contains: Matrix protein p15; Capsid|
           protein p26 (CA) (Major core protein p26); Nucleocapsid
           protein p11; p9]
          Length = 486

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
 Frame = -1

Query: 325 PANGGANK--KQRKEPRCGKCSLTGHIRTQCGRGWESFFR*RGVYHF*KECRDI 170
           P  GGA K    +    C  C   GH+ +QC R  +  F+ +   HF K+CR +
Sbjct: 366 PFKGGALKGGPLKAAQTCYNCGKPGHLSSQC-RAPKVCFKCKQPGHFSKQCRSV 418

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>GAG_EIAVC (P69731) Gag polyprotein [Contains: Matrix protein p15; Capsid|
           protein p26 (CA) (Major core protein p26); Nucleocapsid
           protein p11; p9]
          Length = 486

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
 Frame = -1

Query: 325 PANGGANK--KQRKEPRCGKCSLTGHIRTQCGRGWESFFR*RGVYHF*KECRDI 170
           P  GGA K    +    C  C   GH+ +QC R  +  F+ +   HF K+CR +
Sbjct: 366 PFKGGALKGGPLKAAQTCYNCGKPGHLSSQC-RAPKVCFKCKQPGHFSKQCRSV 418

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>GAG_EIAV9 (P69730) Gag polyprotein [Contains: Matrix protein p15; Capsid|
           protein p26 (CA) (Major core protein p26); Nucleocapsid
           protein p11; p9]
          Length = 486

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
 Frame = -1

Query: 325 PANGGANK--KQRKEPRCGKCSLTGHIRTQCGRGWESFFR*RGVYHF*KECRDI 170
           P  GGA K    +    C  C   GH+ +QC R  +  F+ +   HF K+CR +
Sbjct: 366 PFKGGALKGGPLKAAQTCYNCGKPGHLSSQC-RAPKVCFKCKQPGHFSKQCRSV 418

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>GAG_SIVV1 (P27972) Gag polyprotein [Contains: Core protein p17; Core protein|
           p24; Core protein p15]
          Length = 520

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 10/26 (38%), Positives = 14/26 (53%)
 Frame = -1

Query: 316 GGANKKQRKEPRCGKCSLTGHIRTQC 239
           GG   + R  P+C  C   GH++ QC
Sbjct: 388 GGGRGRPRPPPKCYNCGKFGHMQRQC 413

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>POL_HV2D2 (P15833) Gag-Pol polyprotein (Pr160Gag-Pol) [Contains: Matrix|
           protein p17 (MA); Capsid protein p24 (CA); p2 spacer
           peptide; Nucleocapsid protein* (NC*); Transframe peptide
           (TF) (p6 pol); Protease (EC 3.4.23.47) (Retropepsin)
           (PR); Reverse trans
          Length = 1464

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 15/39 (38%), Positives = 19/39 (48%), Gaps = 3/39 (7%)
 Frame = -1

Query: 313 GANKKQRKEPR---CGKCSLTGHIRTQCGRGWESFFR*R 206
           G   +Q + PR   C KC  TGHI ++C      F R R
Sbjct: 396 GHTARQCRAPRRQGCWKCGKTGHIMSKCPERQAGFLRVR 434

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>DHX15_MOUSE (O35286) Putative pre-mRNA-splicing factor ATP-dependent RNA|
           helicase DHX15 (EC 3.6.1.-) (DEAH box protein 15)
          Length = 795

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 13/33 (39%), Positives = 20/33 (60%)
 Frame = -2

Query: 240 AAVDGNHSFDDEVCIIFKKNAETLSQCHDNAVN 142
           A +DG+H     V   FK+N E++  C+DN +N
Sbjct: 618 AHIDGDHLTLLNVYHAFKQNHESVQWCYDNFIN 650

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>DHX15_HUMAN (O43143) Putative pre-mRNA-splicing factor ATP-dependent RNA|
           helicase DHX15 (EC 3.6.1.-) (DEAH box protein 15)
           (ATP-dependent RNA helicase #46)
          Length = 795

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 13/33 (39%), Positives = 20/33 (60%)
 Frame = -2

Query: 240 AAVDGNHSFDDEVCIIFKKNAETLSQCHDNAVN 142
           A +DG+H     V   FK+N E++  C+DN +N
Sbjct: 618 AHIDGDHLTLLNVYHAFKQNHESVQWCYDNFIN 650

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>AIR2_YEAST (Q12476) Protein AIR2 (Arginine methyltransferase-interacting RING|
           finger protein 2)
          Length = 344

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 10/23 (43%), Positives = 13/23 (56%)
 Frame = -1

Query: 292 KEPRCGKCSLTGHIRTQCGRGWE 224
           K  +C KC   GH R+QC   W+
Sbjct: 97  KAIQCSKCDEVGHYRSQCPHKWK 119

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>GAG_MMTVC (P11284) Gag polyprotein [Contains: Protein p10; Phosphorylated|
           protein pp21; Protein p3; Protein p8; Protein n; Major
           core protein p27; Nucleic acid-binding protein p14]
          Length = 590

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 12/31 (38%), Positives = 14/31 (45%)
 Frame = -1

Query: 331 KSPANGGANKKQRKEPRCGKCSLTGHIRTQC 239
           K    GG   +  K P C  C  TGHI+  C
Sbjct: 509 KQTYGGGKGGQGSKGPVCFSCGKTGHIKRDC 539

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>POL_HV2G1 (P18042) Gag-Pol polyprotein (Pr160Gag-Pol) [Contains: Matrix|
           protein p17 (MA); Capsid protein p24 (CA); p2 spacer
           peptide; Nucleocapsid protein* (NC*); Transframe peptide
           (TF) (p6 pol); Protease (EC 3.4.23.47) (Retropepsin)
           (PR); Reverse trans
          Length = 1463

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 13/37 (35%), Positives = 18/37 (48%), Gaps = 3/37 (8%)
 Frame = -1

Query: 313 GANKKQRKEPR---CGKCSLTGHIRTQCGRGWESFFR 212
           G + +Q + PR   C KC  TGH+  +C      F R
Sbjct: 398 GHSARQCRAPRRQGCWKCGKTGHVMAKCPERQAGFLR 434

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>GAG_SIVVT (P05892) Gag polyprotein [Contains: Core protein p17; Core protein|
           p24; Core protein p15]
          Length = 519

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 11/25 (44%), Positives = 14/25 (56%)
 Frame = -1

Query: 313 GANKKQRKEPRCGKCSLTGHIRTQC 239
           G  K+QR   RC  C   GH++ QC
Sbjct: 388 GGPKRQRPPLRCYNCGKFGHMQRQC 412

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>DEVR_MYXXA (Q07765) Fruiting body developmental protein R|
          Length = 302

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = -3

Query: 323 SKRGSKQEAEEGT-QVR*VQLDRTHQDSMRPWMG 225
           S  G+KQE E+GT +VR   L+ +   S+ PW G
Sbjct: 102 SAEGAKQEKEKGTAKVRRAVLEVSRAVSLTPWSG 135

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>GAG_MMTVB (P10258) Gag polyprotein [Contains: Protein p10; Phosphorylated|
           protein pp21; Protein p3; Protein p8; Protein n; Major
           core protein p27; Nucleic acid-binding protein p14]
          Length = 590

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 12/31 (38%), Positives = 14/31 (45%)
 Frame = -1

Query: 331 KSPANGGANKKQRKEPRCGKCSLTGHIRTQC 239
           K    GG   +  + P C  C  TGHIR  C
Sbjct: 509 KQTYGGGKGGQGAEGPVCFSCGKTGHIRKDC 539

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>EXG_KLULA (Q12628) Glucan 1,3-beta-glucosidase precursor (EC 3.2.1.58)|
           (Exo-1,3-beta-glucanase)
          Length = 429

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 8/28 (28%), Positives = 18/28 (64%)
 Frame = -2

Query: 219 SFDDEVCIIFKKNAETLSQCHDNAVNDW 136
           + D+ + ++ ++  ETL++ H N V +W
Sbjct: 290 TIDEHISVVCEQGKETLTEAHWNVVGEW 317

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>GAG_SIVS4 (P12496) Gag polyprotein [Contains: Core protein p17; Core protein|
           p24]
          Length = 507

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 12/28 (42%), Positives = 16/28 (57%), Gaps = 3/28 (10%)
 Frame = -1

Query: 313 GANKKQRKEPR---CGKCSLTGHIRTQC 239
           G + KQ + PR   C KC  TGH+  +C
Sbjct: 401 GHSAKQCRAPRRQGCWKCGKTGHVMAKC 428

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>NU2M_MYXGL (O21078) NADH-ubiquinone oxidoreductase chain 2 (EC 1.6.5.3) (NADH|
           dehydrogenase subunit 2)
          Length = 348

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 24/91 (26%), Positives = 39/91 (42%), Gaps = 1/91 (1%)
 Frame = -3

Query: 272 VQLDRTH-QDSMRPWMGIILSMTRCVSFLKRMQRHYLNVMIMLLTIGGIKPFVQTIM*FS 96
           ++L+ T   D M  W     S T+C+              ++LL++GG+ PF    +  S
Sbjct: 218 IELNSTKISDLMLSWSKTSWSHTKCI--------------LVLLSLGGLPPFTGFFLKLS 263

Query: 95  VDKCMHAYEGSLICWYLILRICILWHGGLVS 3
           +         +LI   LIL   +L  G L+S
Sbjct: 264 I-------SNALISNSLILMTILLMAGSLIS 287

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>YEW7_YEAST (P40083) Hypothetical 16.6 kDa protein in GDI1-COX15 intergenic|
           region
          Length = 148

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 9/25 (36%), Positives = 15/25 (60%)
 Frame = -1

Query: 310 ANKKQRKEPRCGKCSLTGHIRTQCG 236
           ++KK++ + +C  C   GH R  CG
Sbjct: 115 SSKKKKNKIKCSFCHEAGHTRAHCG 139

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>MAB3_CAEEL (O18214) Protein male abnormal 3|
          Length = 290

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 7/37 (18%)
 Frame = -1

Query: 328 SPANGGANKKQRKEPRCGKCS-------LTGHIRTQC 239
           +PA    + K+ ++P C +CS       L GH RT C
Sbjct: 78  TPATQTRDGKRVRDPHCARCSAHGVLVPLRGHKRTMC 114

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>POLG_HCVED (O39929) Genome polyprotein [Contains: Core protein p21 (Capsid|
           protein C) (p21); Core protein p19; Envelope
           glycoprotein E1 (gp32) (gp35); Envelope glycoprotein E2
           (NS1) (gp68) (gp70); p7; Protease NS2-3 (EC 3.4.22.-)
           (p23); Serine protease/N
          Length = 3007

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 9/12 (75%), Positives = 10/12 (83%)
 Frame = -3

Query: 53  WYLILRICILWH 18
           WY IL ICI+WH
Sbjct: 765 WYAILFICIVWH 776

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>AIR1_YEAST (P40507) Protein AIR1 (Arginine methyltransferase-interacting RING|
           finger protein 1)
          Length = 360

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 8/22 (36%), Positives = 12/22 (54%)
 Frame = -1

Query: 280 CGKCSLTGHIRTQCGRGWESFF 215
           C  C+  GH ++QC   W+  F
Sbjct: 114 CTNCNANGHYKSQCPHKWKKVF 135



 Score = 27.3 bits (59), Expect = 9.7
 Identities = 8/17 (47%), Positives = 11/17 (64%)
 Frame = -1

Query: 289 EPRCGKCSLTGHIRTQC 239
           EP+C  CS  GH++  C
Sbjct: 73  EPKCNNCSQRGHLKRNC 89


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,879,770
Number of Sequences: 219361
Number of extensions: 982591
Number of successful extensions: 2280
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 2208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2280
length of database: 80,573,946
effective HSP length: 87
effective length of database: 61,489,539
effective search space used: 1475748936
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

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full-length cDNA and EST

Clone Information

BLAST Search Result