ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbastl11g02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RPB1_ARATH (P18616) DNA-directed RNA polymerase II largest subun... 37 0.013
2CUT1_SCHPO (P18296) Separin (EC 3.4.22.49) (Separase) (Cell unti... 31 0.70
3RPB1_YEAST (P04050) DNA-directed RNA polymerase II largest subun... 30 1.6
4UBP5_HUMAN (P45974) Ubiquitin carboxyl-terminal hydrolase 5 (EC ... 28 4.5
5RPB1_MOUSE (P08775) DNA-directed RNA polymerase II largest subun... 28 5.9
6RPB1_HUMAN (P24928) DNA-directed RNA polymerase II largest subun... 28 5.9
7RPB1_CRIGR (P11414) DNA-directed RNA polymerase II largest subun... 28 5.9
8LRC25_BOVIN (Q8MII8) Leucine-rich repeat-containing protein 25 p... 28 5.9
9CRIM1_CHICK (Q8AWW5) Cysteine-rich motor neuron 1 protein precur... 28 7.7
10TGBR3_RAT (P26342) TGF-beta receptor type III precursor (TGFR-3)... 28 7.7

>RPB1_ARATH (P18616) DNA-directed RNA polymerase II largest subunit (EC 2.7.7.6)|
          Length = 1840

 Score = 37.0 bits (84), Expect = 0.013
 Identities = 18/27 (66%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -2

Query: 298  YSPTAPGYSPSSTG---PQATDKDDET 227
            YSPT PGYSPSSTG   P   DK D+T
Sbjct: 1801 YSPTLPGYSPSSTGQYTPHEGDKKDKT 1827



 Score = 30.0 bits (66), Expect = 1.6
 Identities = 12/18 (66%), Positives = 14/18 (77%)
 Frame = -2

Query: 298  YSPTAPGYSPSSTGPQAT 245
            YSPT+PGYSP+S G   T
Sbjct: 1559 YSPTSPGYSPTSPGYSPT 1576



 Score = 30.0 bits (66), Expect = 1.6
 Identities = 12/18 (66%), Positives = 14/18 (77%)
 Frame = -2

Query: 298  YSPTAPGYSPSSTGPQAT 245
            YSPT+PGYSP+S G   T
Sbjct: 1552 YSPTSPGYSPTSPGYSPT 1569



 Score = 28.5 bits (62), Expect = 4.5
 Identities = 11/18 (61%), Positives = 14/18 (77%)
 Frame = -2

Query: 298  YSPTAPGYSPSSTGPQAT 245
            YSP++PGYSP+S G   T
Sbjct: 1545 YSPSSPGYSPTSPGYSPT 1562



 Score = 28.1 bits (61), Expect = 5.9
 Identities = 12/18 (66%), Positives = 13/18 (72%)
 Frame = -2

Query: 298  YSPTAPGYSPSSTGPQAT 245
            YSPT+P YSPSS G   T
Sbjct: 1573 YSPTSPTYSPSSPGYSPT 1590



 Score = 27.7 bits (60), Expect = 7.7
 Identities = 10/12 (83%), Positives = 12/12 (100%)
 Frame = -2

Query: 298  YSPTAPGYSPSS 263
            YSPT+PGYSP+S
Sbjct: 1699 YSPTSPGYSPTS 1710



 Score = 27.7 bits (60), Expect = 7.7
 Identities = 11/18 (61%), Positives = 13/18 (72%)
 Frame = -2

Query: 298  YSPTAPGYSPSSTGPQAT 245
            YSPT+P YSP+S G   T
Sbjct: 1692 YSPTSPAYSPTSPGYSPT 1709



 Score = 27.7 bits (60), Expect = 7.7
 Identities = 10/12 (83%), Positives = 12/12 (100%)
 Frame = -2

Query: 298  YSPTAPGYSPSS 263
            YSPT+PGYSP+S
Sbjct: 1566 YSPTSPGYSPTS 1577



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>CUT1_SCHPO (P18296) Separin (EC 3.4.22.49) (Separase) (Cell untimely torn|
            protein 1)
          Length = 1828

 Score = 31.2 bits (69), Expect = 0.70
 Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 3/46 (6%)
 Frame = -2

Query: 247  TDKDDETVD*KMAADRWLMQNHATLPTLVSLCV---QNRPCCKLRY 119
            TDKD +    KM     L +N A      S+C    ++R CC LRY
Sbjct: 1766 TDKDIDRFSLKMLESWGLFENKAPFVNSTSICTAVSESRSCCHLRY 1811



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>RPB1_YEAST (P04050) DNA-directed RNA polymerase II largest subunit (EC 2.7.7.6)|
            (RNA polymerase II subunit 1) (B220)
          Length = 1733

 Score = 30.0 bits (66), Expect = 1.6
 Identities = 14/23 (60%), Positives = 16/23 (69%)
 Frame = -2

Query: 298  YSPTAPGYSPSSTGPQATDKDDE 230
            YSPT+PGYSP S  P  + K DE
Sbjct: 1703 YSPTSPGYSPGS--PAYSPKQDE 1723



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>UBP5_HUMAN (P45974) Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15)|
           (Ubiquitin thioesterase 5)
           (Ubiquitin-specific-processing protease 5)
           (Deubiquitinating enzyme 5) (Isopeptidase T)
          Length = 858

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 12/37 (32%), Positives = 18/37 (48%)
 Frame = +1

Query: 154 TRKQELVMWHGSASASGQQPFSNQQSRRPCQLPADRW 264
           +RKQE+  W G      +  FS +Q   P ++P   W
Sbjct: 161 SRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGW 197



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>RPB1_MOUSE (P08775) DNA-directed RNA polymerase II largest subunit (EC 2.7.7.6)|
            (RPB1)
          Length = 1970

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 13/28 (46%), Positives = 17/28 (60%), Gaps = 5/28 (17%)
 Frame = -2

Query: 298  YSPTAPGYSPSS-----TGPQATDKDDE 230
            YSPT+PGYSP+S     T P  +  D +
Sbjct: 1940 YSPTSPGYSPTSPTYSLTSPAISPDDSD 1967



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>RPB1_HUMAN (P24928) DNA-directed RNA polymerase II largest subunit (EC 2.7.7.6)|
            (RPB1)
          Length = 1970

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 13/28 (46%), Positives = 17/28 (60%), Gaps = 5/28 (17%)
 Frame = -2

Query: 298  YSPTAPGYSPSS-----TGPQATDKDDE 230
            YSPT+PGYSP+S     T P  +  D +
Sbjct: 1940 YSPTSPGYSPTSPTYSLTSPAISPDDSD 1967



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>RPB1_CRIGR (P11414) DNA-directed RNA polymerase II largest subunit (EC|
           2.7.7.6) (RPB1) (Fragment)
          Length = 467

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 13/28 (46%), Positives = 17/28 (60%), Gaps = 5/28 (17%)
 Frame = -2

Query: 298 YSPTAPGYSPSS-----TGPQATDKDDE 230
           YSPT+PGYSP+S     T P  +  D +
Sbjct: 437 YSPTSPGYSPTSPTYSLTSPAISPDDSD 464



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>LRC25_BOVIN (Q8MII8) Leucine-rich repeat-containing protein 25 precursor|
           (Monocyte and plasmacytoid-activated protein)
          Length = 307

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 15/33 (45%), Positives = 21/33 (63%)
 Frame = +1

Query: 184 GSASASGQQPFSNQQSRRPCQLPADRWTKGSSP 282
           GS S SG+QP  + Q RRP + PA+   + S+P
Sbjct: 211 GSRSGSGRQPRYSSQGRRP-KSPANTPPRSSTP 242



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>CRIM1_CHICK (Q8AWW5) Cysteine-rich motor neuron 1 protein precursor (CRIM-1)|
          Length = 1048

 Score = 27.7 bits (60), Expect = 7.7
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -1

Query: 209 C*PLADAEPCHITNSCFLVCPE 144
           C PL  AEP ++  SC  +CPE
Sbjct: 866 CPPLPCAEPINVEGSCCPMCPE 887



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>TGBR3_RAT (P26342) TGF-beta receptor type III precursor (TGFR-3)|
           (Transforming growth factor beta receptor III)
           (Betaglycan)
          Length = 853

 Score = 27.7 bits (60), Expect = 7.7
 Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
 Frame = +2

Query: 200 AVSSHFLINSLVVLVS-CLRTGGRRGVARC 286
           AV+SH +I  +VVL+S CL T G     RC
Sbjct: 2   AVTSHHMIPVMVVLMSACLATAGPEPSTRC 31


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,436,433
Number of Sequences: 219361
Number of extensions: 645754
Number of successful extensions: 1958
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1556
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1951
length of database: 80,573,946
effective HSP length: 75
effective length of database: 64,121,871
effective search space used: 1538924904
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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