Clone Name | rbart59a07 |
---|---|
Clone Library Name | barley_pub |
>gb|AF307145.1| Hordeum vulgare subsp. vulgare glutamine-dependent asparagine synthetase 1 mRNA, complete cds Length = 1973 Score = 797 bits (402), Expect = 0.0 Identities = 426/430 (99%), Gaps = 3/430 (0%) Strand = Plus / Minus Query: 1 agaaatcatccggtctctgatttcataagaactatagaaacattaatgcacaatcttcac 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1973 agaaatcatccggtctctgatttcataagaactatagaaacattaatgcacaatcttcac 1914 Query: 61 tcaaccttaggtctgcatcactctaatggcaagactaggtacaccttatttcttatcaga 120 ||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||| Sbjct: 1913 tcaaccttaggtctgcatcactctaatggcaagactaggtacatcttatttcttatcaga 1854 Query: 121 aacggcaagcaggacaggacaccatcaactctcaattgcgacaccaggtgccgcaacctt 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1853 aacggcaagcaggacaggacaccatcaactctcaattgcgacaccaggtgccgcaacctt 1794 Query: 181 gatcatcctcggcttcttgctggttcctgccatgatggttgctgggagatgctcctgctc 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1793 gatcatcctcggcttcttgctggttcctgccatgatggttgctgggagatgctcctgctc 1734 Query: 241 ataggccgagagatggactccaagtgctgctctccctgagggatccaggttccccgacca 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1733 ataggccgagagatggactccaagtgctgctctccctgagggatccaggttccccgacca 1674 Query: 301 ccgagcatcccactctactgccttcgccgtgctgcatgcgacgcttggcccgcctggcac 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1673 ccgagcatcccactctactgccttcgccgtgctgcatgcgacgcttggcccgcctggcac 1614 Query: 361 tgtcaggattgccgagttctgggggaag-acctctc-aatatcatcctgt-acagtaggc 417 |||||||||||||||||||||||||||| ||||||| ||||||||||||| ||||||||| Sbjct: 1613 tgtcaggattgccgagttctgggggaagaacctctcaaatatcatcctgtaacagtaggc 1554 Query: 418 ctcttttgta 427 |||||||||| Sbjct: 1553 ctcttttgta 1544
>gb|BT009245.1| Triticum aestivum clone wlk1.pk0018.d4:fis, full insert mRNA sequence Length = 2064 Score = 656 bits (331), Expect = 0.0 Identities = 410/431 (95%), Gaps = 4/431 (0%) Strand = Plus / Minus Query: 1 agaaatcatccggtctctgatttcataagaactatagaaacattaatgcacaatcttcac 60 ||||||||||||||||| |||||||||||||| ||||||||||||||||||||||||||| Sbjct: 1958 agaaatcatccggtctccgatttcataagaacaatagaaacattaatgcacaatcttcac 1899 Query: 61 tcaa-ccttaggtctgcatcactctaatggcaagactaggtacaccttatttcttatcag 119 |||| ||||||||||||| ||||||||||||||||| ||||||| ||||||||||||||| Sbjct: 1898 tcaaaccttaggtctgcaccactctaatggcaagaccaggtacatcttatttcttatcag 1839 Query: 120 aaacggcaagcaggacaggacaccatcaactctcaattgcgacaccaggtgccgcaacct 179 ||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| Sbjct: 1838 aaacggcaagcaggacaggacaccatcaactctcaattgcgacaccaggcgccgcaacct 1779 Query: 180 tgatcatcctcggcttcttgctggttcctgccatgatggttgctgggagatgctcctgct 239 ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| | Sbjct: 1778 cgatcatccttggcttcttgctggttcctgccatgatggttgctgggagatgctcctgtt 1719 Query: 240 cataggccgagagatggactccaagtgctgctctccctgagggatccaggttccccgacc 299 ||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||| Sbjct: 1718 cataggccgagagatggactccaagtgctgctctccctgagggatccaggttccctgacc 1659 Query: 300 accgagcatcccactctactgccttcgccgtgctgcatgcgacgcttggcccgcctggca 359 || | |||||||||||||| |||||||||||||||||||| ||||||||||| ||||||| Sbjct: 1658 actgggcatcccactctaccgccttcgccgtgctgcatgcaacgcttggcccacctggca 1599 Query: 360 ctgtcaggattgccgagttctgggggaag-acctctc-aatatcatcctgt-acagtagg 416 | |||||||| |||||||||||||||||| ||||||| ||||||||||||| |||||||| Sbjct: 1598 ccgtcaggatcgccgagttctgggggaagaacctctcaaatatcatcctgtaacagtagg 1539 Query: 417 cctcttttgta 427 ||||||||||| Sbjct: 1538 cctcttttgta 1528
>gb|AY621539.1| Triticum aestivum glutamine-dependent asparagine synthetase (ASN1) mRNA, complete cds Length = 1815 Score = 474 bits (239), Expect = e-130 Identities = 293/307 (95%), Gaps = 3/307 (0%) Strand = Plus / Minus Query: 124 ggcaagcaggacaggacaccatcaactctcaattgcgacaccaggtgccgcaaccttgat 183 ||||||||||||||||||||||||||||||||||||||||||||| |||||||||| ||| Sbjct: 1800 ggcaagcaggacaggacaccatcaactctcaattgcgacaccaggcgccgcaacctcgat 1741 Query: 184 catcctcggcttcttgctggttcctgccatgatggttgctgggagatgctcctgctcata 243 |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| Sbjct: 1740 catcctcggcttcttgctggttcctgccatgatggttgctgggagatgctcctgttcata 1681 Query: 244 ggccgagagatggactccaagtgctgctctccctgagggatccaggttccccgaccaccg 303 ||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| | Sbjct: 1680 ggccgagagatggactccaagtgctgctctccctgagggatccaggttccctgaccactg 1621 Query: 304 agcatcccactctactgccttcgccgtgctgcatgcgacgcttggcccgcctggcactgt 363 |||||||||||||| |||||||||||||||||||| ||||||||||| |||||||| || Sbjct: 1620 ggcatcccactctaccgccttcgccgtgctgcatgcaacgcttggcccacctggcaccgt 1561 Query: 364 caggattgccgagttctgggggaag-acctctc-aatatcatcctgt-acagtaggcctc 420 |||||| |||||||||||||||||| ||||||| ||||||||||||| |||||||||||| Sbjct: 1560 caggatcgccgagttctgggggaagaacctctcaaatatcatcctgtaacagtaggcctc 1501 Query: 421 ttttgta 427 ||||||| Sbjct: 1500 ttttgta 1494
>gb|AY193714.1| Hordeum vulgare asparagine synthetase 2 (AS2) mRNA, complete cds Length = 1867 Score = 170 bits (86), Expect = 2e-39 Identities = 170/194 (87%), Gaps = 3/194 (1%) Strand = Plus / Minus Query: 233 tcctgctcataggccgagagatggactccaagtgctgctctccctgagggatccaggttc 292 |||||||| ||||| ||||||||||||||||| ||||||||||| ||||| ||||||||| Sbjct: 1670 tcctgctcgtaggctgagagatggactccaagcgctgctctcccagaggggtccaggttc 1611 Query: 293 cccgaccaccgagcatcccactctactgccttcgccgtgctgcatgcgacgcttggcccg 352 || |||||| | || |||||||||| |||||| || |||||||| || |||||||||||| Sbjct: 1610 ccagaccactgcgcgtcccactctattgccttggctgtgctgcacgccacgcttggcccg 1551 Query: 353 cctggcactgtcaggattgccgagttctgggggaag-acctctc-aatatcatcctgt-a 409 |||||||| || ||||| |||||| |||| |||||| ||||||| || |||||||||| | Sbjct: 1550 cctggcaccgtgaggatcgccgagctctgagggaagtacctctcgaagatcatcctgtaa 1491 Query: 410 cagtaggcctcttt 423 ||||||||||||| Sbjct: 1490 tagtaggcctcttt 1477
>gb|BT009049.1| Triticum aestivum clone wdk9n1.pk001.f6:fis, full insert mRNA sequence Length = 2119 Score = 170 bits (86), Expect = 2e-39 Identities = 170/194 (87%), Gaps = 3/194 (1%) Strand = Plus / Minus Query: 233 tcctgctcataggccgagagatggactccaagtgctgctctccctgagggatccaggttc 292 |||||||| ||||| ||||||||||||||||| ||||||||||| ||||| ||||||||| Sbjct: 1739 tcctgctcgtaggctgagagatggactccaagcgctgctctcccagaggggtccaggttc 1680 Query: 293 cccgaccaccgagcatcccactctactgccttcgccgtgctgcatgcgacgcttggcccg 352 || ||||| | ||||||||||||| ||||| || |||||||| || |||||||||||| Sbjct: 1679 ccagaccattgggcatcccactctatagccttggctgtgctgcacgccacgcttggcccg 1620 Query: 353 cctggcactgtcaggattgccgagttctgggggaag-acctctc-aatatcatcctgt-a 409 |||||||| || ||||| |||||| ||||||||||| ||||||| || |||||||||| | Sbjct: 1619 cctggcaccgtgaggatcgccgagctctgggggaagtacctctcaaagatcatcctgtaa 1560 Query: 410 cagtaggcctcttt 423 ||||||||||||| Sbjct: 1559 tagtaggcctcttt 1546
>gb|AF190729.1| Helianthus annuus asparagine synthetase (HAS2) mRNA, complete cds Length = 1850 Score = 58.0 bits (29), Expect = 2e-05 Identities = 47/53 (88%) Strand = Plus / Minus Query: 305 gcatcccactctactgccttcgccgtgctgcatgcgacgcttggcccgcctgg 357 |||||||||||||| ||||| |||||||||||||| || ||||| || ||||| Sbjct: 1623 gcatcccactctaccgccttagccgtgctgcatgccacacttggaccccctgg 1571
>emb|X89409.1|LJAS1GENE L.japonicus mRNA for asparagine synthetase (clone LJAS1) Length = 2053 Score = 50.1 bits (25), Expect = 0.006 Identities = 73/89 (82%) Strand = Plus / Minus Query: 251 agatggactccaagtgctgctctccctgagggatccaggttccccgaccaccgagcatcc 310 ||||| ||||| ||||||||||| ||||| ||||| ||||| ||||| ||||||| Sbjct: 1750 agatgcactcctagtgctgctctacctgaaggatcaaggttattagaccaagcagcatcc 1691 Query: 311 cactctactgccttcgccgtgctgcatgc 339 ||||| |||||||| || ||||| ||||| Sbjct: 1690 cactcaactgcctttgctgtgctacatgc 1662
>emb|Z72354.1|VFAS1 V.faba mRNA for asparagine synthetase Length = 2154 Score = 46.1 bits (23), Expect = 0.093 Identities = 104/131 (79%) Strand = Plus / Minus Query: 257 actccaagtgctgctctccctgagggatccaggttccccgaccaccgagcatcccactct 316 ||||||||||||||||| || || ||||| ||||| || ||||| ||||||||| || Sbjct: 1766 actccaagtgctgctctaccagatggatcaaggttgcctgaccaagaagcatcccattca 1707 Query: 317 actgccttcgccgtgctgcatgcgacgcttggcccgcctggcactgtcaggattgccgag 376 | || || | ||||||||||| || || || || || || |||||||| |||||||| Sbjct: 1706 atagctttttctgtgctgcatgcaacactaggtcctccaggaactgtcagccttgccgag 1647 Query: 377 ttctgggggaa 387 ||||| ||||| Sbjct: 1646 ttctgagggaa 1636
>emb|X52179.1|PSAS1 Pea AS1 nodule mRNA for asparagine synthase (EC 6.3.5.4) Length = 2200 Score = 46.1 bits (23), Expect = 0.093 Identities = 32/35 (91%) Strand = Plus / Minus Query: 257 actccaagtgctgctctccctgagggatccaggtt 291 ||||||||||||||||| || || ||||||||||| Sbjct: 1742 actccaagtgctgctctaccagaaggatccaggtt 1708
>emb|AJ133522.1|PVU133522 Phaseolus vulgaris mRNA for asparagine synthetase type-I Length = 2086 Score = 46.1 bits (23), Expect = 0.093 Identities = 32/35 (91%) Strand = Plus / Minus Query: 257 actccaagtgctgctctccctgagggatccaggtt 291 ||||||||||||||||| ||||| ||||| ||||| Sbjct: 1793 actccaagtgctgctctacctgatggatcaaggtt 1759
>emb|AJ496567.2|PSY496567 Pinus sylvestris mRNA for putative asparagine synthetase (asn1 gene) Length = 2110 Score = 46.1 bits (23), Expect = 0.093 Identities = 44/51 (86%) Strand = Plus / Minus Query: 304 agcatcccactctactgccttcgccgtgctgcatgcgacgcttggcccgcc 354 ||||||||| || |||||||| || ||||| || || |||||||||||||| Sbjct: 1691 agcatcccattccactgcctttgcggtgctacaagccacgcttggcccgcc 1641
>emb|CT573508.2| Medicago truncatula chromosome 5 clone mth2-29p15, COMPLETE SEQUENCE Length = 125647 Score = 46.1 bits (23), Expect = 0.093 Identities = 68/83 (81%) Strand = Plus / Minus Query: 257 actccaagtgctgctctccctgagggatccaggttccccgaccaccgagcatcccactct 316 ||||||||||||||||| || || ||||| |||||| ||||| |||||||||||| Sbjct: 7521 actccaagtgctgctctaccagaaggatcaaggttcttggaccaagaagcatcccactca 7462 Query: 317 actgccttcgccgtgctgcatgc 339 || ||||| || ||||| ||||| Sbjct: 7461 acagccttagctgtgctacatgc 7439
>gb|U77679.1|GMU77679 Glycine max asparagine synthetase 1 (AS1) mRNA, complete cds Length = 2108 Score = 46.1 bits (23), Expect = 0.093 Identities = 32/35 (91%) Strand = Plus / Minus Query: 257 actccaagtgctgctctccctgagggatccaggtt 291 ||||||||||||||||| ||||| ||||| ||||| Sbjct: 1796 actccaagtgctgctctacctgatggatcaaggtt 1762
>gb|U77678.1|GMU77678 Glycine max asparagine synthetase 2 (AS2) mRNA, complete cds Length = 2143 Score = 46.1 bits (23), Expect = 0.093 Identities = 32/35 (91%) Strand = Plus / Minus Query: 257 actccaagtgctgctctccctgagggatccaggtt 291 ||||||||||||||||| || |||||||| ||||| Sbjct: 1800 actccaagtgctgctctaccagagggatcaaggtt 1766
>gb|AC099500.2| Homo sapiens chromosome 19 clone RP11-209J6, complete sequence Length = 138627 Score = 42.1 bits (21), Expect = 1.5 Identities = 21/21 (100%) Strand = Plus / Minus Query: 380 tgggggaagacctctcaatat 400 ||||||||||||||||||||| Sbjct: 71555 tgggggaagacctctcaatat 71535
>gb|CP000133.1| Rhizobium etli CFN 42, complete genome Length = 4381608 Score = 42.1 bits (21), Expect = 1.5 Identities = 21/21 (100%) Strand = Plus / Minus Query: 298 ccaccgagcatcccactctac 318 ||||||||||||||||||||| Sbjct: 3292410 ccaccgagcatcccactctac 3292390
>gb|AC139769.1| Homo sapiens chromosome 19 clone RP11-255H23, complete sequence Length = 136449 Score = 42.1 bits (21), Expect = 1.5 Identities = 21/21 (100%) Strand = Plus / Minus Query: 380 tgggggaagacctctcaatat 400 ||||||||||||||||||||| Sbjct: 22597 tgggggaagacctctcaatat 22577
>gb|AC138126.1| Homo sapiens chromosome 19 clone RP11-274A19, complete sequence Length = 169063 Score = 42.1 bits (21), Expect = 1.5 Identities = 21/21 (100%) Strand = Plus / Minus Query: 380 tgggggaagacctctcaatat 400 ||||||||||||||||||||| Sbjct: 102424 tgggggaagacctctcaatat 102404
>gb|CP000031.1| Silicibacter pomeroyi DSS-3, complete genome Length = 4109442 Score = 40.1 bits (20), Expect = 5.8 Identities = 20/20 (100%) Strand = Plus / Plus Query: 228 gatgctcctgctcataggcc 247 |||||||||||||||||||| Sbjct: 2224484 gatgctcctgctcataggcc 2224503
>emb|AL139385.12| Human DNA sequence from clone RP11-90L1 on chromosome 13 Contains the 3' end of the COL4A2 gene for collagen type IV alpha 2, the RAB20 gene for a RAS oncogene family member, a novel gene (FLJ10769 FLJ25038 FLJ14198), the 5' end of a novel gene (FLJ12118) and three CpG islands, complete sequence Length = 177392 Score = 40.1 bits (20), Expect = 5.8 Identities = 23/24 (95%) Strand = Plus / Plus Query: 195 tcttgctggttcctgccatgatgg 218 |||||||| ||||||||||||||| Sbjct: 8457 tcttgctgtttcctgccatgatgg 8480
>emb|Y10196.1|HSPEX Homo sapiens PHEX gene Length = 242825 Score = 40.1 bits (20), Expect = 5.8 Identities = 20/20 (100%) Strand = Plus / Plus Query: 32 ctatagaaacattaatgcac 51 |||||||||||||||||||| Sbjct: 22051 ctatagaaacattaatgcac 22070
>emb|X99552.1|AOASPS A.officinalis gene encoding asparagine synthetase Length = 3812 Score = 40.1 bits (20), Expect = 5.8 Identities = 68/84 (80%) Strand = Plus / Minus Query: 253 atggactccaagtgctgctctccctgagggatccaggttccccgaccaccgagcatccca 312 |||||| || || ||||||||||| || ||||||| || |||||| ||||| ||||| Sbjct: 3689 atggacgccgagagctgctctccccgacggatccaaattgttcgaccagcgagcgtccca 3630 Query: 313 ctctactgccttcgccgtgctgca 336 || | ||||||||||||||||| Sbjct: 3629 ttcgatagccttcgccgtgctgca 3606
>emb|X67958.1|AOASPSYNM A.officinalis mRNA for asparagine synthetase Length = 1978 Score = 40.1 bits (20), Expect = 5.8 Identities = 68/84 (80%) Strand = Plus / Minus Query: 253 atggactccaagtgctgctctccctgagggatccaggttccccgaccaccgagcatccca 312 |||||| || || ||||||||||| || ||||||| || |||||| ||||| ||||| Sbjct: 1705 atggacgccgagagctgctctccccgacggatccaaattgttcgaccagcgagcgtccca 1646 Query: 313 ctctactgccttcgccgtgctgca 336 || | ||||||||||||||||| Sbjct: 1645 ttcgatagccttcgccgtgctgca 1622
>emb|CR932777.1| Human DNA sequence from clone XX-NCIH2171_7H20, complete sequence Length = 115459 Score = 40.1 bits (20), Expect = 5.8 Identities = 20/20 (100%) Strand = Plus / Minus Query: 56 ttcactcaaccttaggtctg 75 |||||||||||||||||||| Sbjct: 16589 ttcactcaaccttaggtctg 16570
>gb|AC087692.7| Homo sapiens chromosome 8, clone RP11-67D13, complete sequence Length = 146549 Score = 40.1 bits (20), Expect = 5.8 Identities = 23/24 (95%) Strand = Plus / Plus Query: 264 gtgctgctctccctgagggatcca 287 |||||||||||| ||||||||||| Sbjct: 3400 gtgctgctctccatgagggatcca 3423
>gb|AC084880.12| Homo sapiens 12 BAC RP11-64B16 (Roswell Park Cancer Institute Human BAC Library) complete sequence Length = 110001 Score = 40.1 bits (20), Expect = 5.8 Identities = 23/24 (95%) Strand = Plus / Minus Query: 17 ctgatttcataagaactatagaaa 40 ||||| |||||||||||||||||| Sbjct: 64664 ctgatctcataagaactatagaaa 64641
>gb|AC021523.8| Homo sapiens chromosome 8, clone RP11-336N16, complete sequence Length = 170299 Score = 40.1 bits (20), Expect = 5.8 Identities = 23/24 (95%) Strand = Plus / Plus Query: 264 gtgctgctctccctgagggatcca 287 |||||||||||| ||||||||||| Sbjct: 156531 gtgctgctctccatgagggatcca 156554
>gb|AC092755.4| Homo sapiens chromosome 15 clone RP11-361D15 map 15q22, complete sequence Length = 187429 Score = 40.1 bits (20), Expect = 5.8 Identities = 20/20 (100%) Strand = Plus / Plus Query: 65 ccttaggtctgcatcactct 84 |||||||||||||||||||| Sbjct: 14165 ccttaggtctgcatcactct 14184
>gb|AC092754.4| Homo sapiens chromosome 15 clone RP11-112N19 map 15q22, complete sequence Length = 173411 Score = 40.1 bits (20), Expect = 5.8 Identities = 20/20 (100%) Strand = Plus / Plus Query: 65 ccttaggtctgcatcactct 84 |||||||||||||||||||| Sbjct: 152367 ccttaggtctgcatcactct 152386
>gb|U73024.1|U73024 Homo sapiens cosmid clone U54B12 from Xp22.1-22.2, complete sequence Length = 38476 Score = 40.1 bits (20), Expect = 5.8 Identities = 20/20 (100%) Strand = Plus / Plus Query: 32 ctatagaaacattaatgcac 51 |||||||||||||||||||| Sbjct: 31688 ctatagaaacattaatgcac 31707
>gb|AC005344.1| Homo sapiens 12 PAC RPCI1-53O8 (Roswell Park Cancer Institute Human PAC library) complete sequence Length = 153836 Score = 40.1 bits (20), Expect = 5.8 Identities = 20/20 (100%) Strand = Plus / Minus Query: 56 ttcactcaaccttaggtctg 75 |||||||||||||||||||| Sbjct: 21723 ttcactcaaccttaggtctg 21704
>gb|AC132436.3| Mus musculus BAC clone RP23-226J18 from 1, complete sequence Length = 248067 Score = 40.1 bits (20), Expect = 5.8 Identities = 23/24 (95%) Strand = Plus / Minus Query: 264 gtgctgctctccctgagggatcca 287 ||||||||||||| |||||||||| Sbjct: 165502 gtgctgctctcccagagggatcca 165479 Database: nt Posted date: May 29, 2006 11:10 AM Number of letters in database: 3,984,495,279 Number of sequences in database: 917,343 Database: /shigen/export/home/twatanab/db/nt/nt.01 Posted date: May 29, 2006 11:16 AM Number of letters in database: 3,988,174,986 Number of sequences in database: 835,257 Database: /shigen/export/home/twatanab/db/nt/nt.02 Posted date: May 29, 2006 11:21 AM Number of letters in database: 3,991,246,324 Number of sequences in database: 771,481 Database: /shigen/export/home/twatanab/db/nt/nt.03 Posted date: May 29, 2006 11:27 AM Number of letters in database: 3,990,718,311 Number of sequences in database: 977,174 Database: /shigen/export/home/twatanab/db/nt/nt.04 Posted date: May 29, 2006 11:29 AM Number of letters in database: 1,278,410,368 Number of sequences in database: 400,813 Lambda K H 1.37 0.711 1.31 Gapped Lambda K H 1.37 0.711 1.31 Matrix: blastn matrix:1 -3 Gap Penalties: Existence: 5, Extension: 2 Number of Hits to DB: 3,411,054 Number of Sequences: 3902068 Number of extensions: 3411054 Number of successful extensions: 60500 Number of sequences better than 10.0: 32 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 60410 Number of HSP's gapped (non-prelim): 82 length of query: 429 length of database: 17,233,045,268 effective HSP length: 22 effective length of query: 407 effective length of database: 17,147,199,772 effective search space: 6978910307204 effective search space used: 6978910307204 T: 0 A: 0 X1: 6 (11.9 bits) X2: 15 (29.7 bits) S1: 12 (24.3 bits) S2: 20 (40.1 bits)