ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart55e01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1HMT3_MAIZE (Q9FUM8) Homocysteine S-methyltransferase 3 (EC 2.1.1... 65 6e-11
2HMT2_MAIZE (Q9FUM9) Homocysteine S-methyltransferase 2 (EC 2.1.1... 62 5e-10
3SMTA_ASTBI (P56707) Selenocysteine methyltransferase (EC 2.1.1.-... 49 7e-06
4HMT4_MAIZE (Q9FUM7) Homocysteine S-methyltransferase 4 (EC 2.1.1... 48 1e-05
5HMT3_ARATH (Q8LAX0) Homocysteine S-methyltransferase 3 (EC 2.1.1... 47 3e-05
6HMT1_MAIZE (Q9FUN0) Homocysteine S-methyltransferase 1 (EC 2.1.1... 46 4e-05
7HMT1_ARATH (Q9SDL7) Homocysteine S-methyltransferase 1 (EC 2.1.1... 45 8e-05
8HMT2_ARATH (Q9M1W4) Homocysteine S-methyltransferase 2 (EC 2.1.1... 43 4e-04
9YM99_YEAST (Q04898) Hypothetical protein YMR321c 38 0.013
10SAM4_YEAST (Q08985) Homocysteine S-methyltransferase 2 (EC 2.1.1... 38 0.013
11MMUM_ECOLI (Q47690) Homocysteine S-methyltransferase (EC 2.1.1.1... 36 0.037
12MHT1_YEAST (Q12525) Homocysteine S-methyltransferase 1 (EC 2.1.1... 35 0.063
13METH_VIBCH (Q9KUW9) Methionine synthase (EC 2.1.1.13) (5-methylt... 34 0.14
14METH_VIBPA (Q87L95) Methionine synthase (EC 2.1.1.13) (5-methylt... 34 0.18
15METH_ECOLI (P13009) Methionine synthase (EC 2.1.1.13) (5-methylt... 33 0.24
16METH_SALTY (P37586) Methionine synthase (EC 2.1.1.13) (5-methylt... 33 0.31
17METH_PSEPU (O33465) Methionine synthase (EC 2.1.1.13) (5-methylt... 32 0.53
18METH_VIBVU (Q8DCJ7) Methionine synthase (EC 2.1.1.13) (5-methylt... 32 0.70
19METH_VIBVY (Q7MHB1) Methionine synthase (EC 2.1.1.13) (5-methylt... 32 0.91
20METH_MYCLE (Q49775) Methionine synthase (EC 2.1.1.13) (5-methylt... 32 0.91
21METH_CAEEL (Q09582) Probable methionine synthase (EC 2.1.1.13) (... 32 0.91
22METH_VIBFI (Q9AJQ8) Methionine synthase (EC 2.1.1.13) (5-methylt... 31 1.6
23METH_VIBF1 (Q5E814) Methionine synthase (EC 2.1.1.13) (5-methylt... 31 1.6
24METH_MYCTU (O33259) Methionine synthase (EC 2.1.1.13) (5-methylt... 30 2.0
25ARLY_PROMM (Q7TV86) Argininosuccinate lyase (EC 4.3.2.1) (Argino... 29 4.5
26METH_HUMAN (Q99707) Methionine synthase (EC 2.1.1.13) (5-methylt... 29 4.5
27NUD10_ARATH (Q6NPD7) Nudix hydrolase 10 (EC 3.6.1.-) (AtNUDT10) ... 28 7.7

>HMT3_MAIZE (Q9FUM8) Homocysteine S-methyltransferase 3 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 3)
           (SMM:Hcy S-methyltransferase 3) (ZmHMT-3)
          Length = 338

 Score = 65.5 bits (158), Expect = 6e-11
 Identities = 28/30 (93%), Positives = 29/30 (96%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSLNQ 354
           EWCKDGAALIGGCCRTTPNTIRAI R+LNQ
Sbjct: 299 EWCKDGAALIGGCCRTTPNTIRAIHRTLNQ 328



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>HMT2_MAIZE (Q9FUM9) Homocysteine S-methyltransferase 2 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 2)
           (SMM:Hcy S-methyltransferase 2) (ZmHMT-2)
          Length = 339

 Score = 62.4 bits (150), Expect = 5e-10
 Identities = 26/30 (86%), Positives = 28/30 (93%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSLNQ 354
           EWCKDGA LIGGCCRTTPNTIRAI R+LN+
Sbjct: 299 EWCKDGAVLIGGCCRTTPNTIRAIHRTLNK 328



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>SMTA_ASTBI (P56707) Selenocysteine methyltransferase (EC 2.1.1.-)|
           (SECYS-methyltransferase) (SECYS-MT)
          Length = 338

 Score = 48.5 bits (114), Expect = 7e-06
 Identities = 18/29 (62%), Positives = 25/29 (86%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSLN 357
           +WC+ GA+L+GGCCRTTP+TIR I + L+
Sbjct: 300 KWCESGASLVGGCCRTTPDTIRGIYKILS 328



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>HMT4_MAIZE (Q9FUM7) Homocysteine S-methyltransferase 4 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 4)
           (SMM:Hcy S-methyltransferase 4) (ZmHMT-4)
          Length = 342

 Score = 47.8 bits (112), Expect = 1e-05
 Identities = 19/30 (63%), Positives = 26/30 (86%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSLNQ 354
           EW + GAALIGGCCRT+P T+RAI+R++ +
Sbjct: 301 EWRRAGAALIGGCCRTSPATVRAIARAVRE 330



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>HMT3_ARATH (Q8LAX0) Homocysteine S-methyltransferase 3 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 3)
           (SMM:Hcy S-methyltransferase 3) (AtHMT-3)
          Length = 347

 Score = 46.6 bits (109), Expect = 3e-05
 Identities = 21/35 (60%), Positives = 26/35 (74%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSLNQYYPAA 339
           +W   GA+L GGCCRTTPNTIRAI++ L+    AA
Sbjct: 306 KWRDAGASLFGGCCRTTPNTIRAIAKVLSDEPSAA 340



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>HMT1_MAIZE (Q9FUN0) Homocysteine S-methyltransferase 1 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 1)
           (SMM:Hcy S-methyltransferase 1) (ZmHMT-1)
          Length = 323

 Score = 46.2 bits (108), Expect = 4e-05
 Identities = 19/27 (70%), Positives = 24/27 (88%)
 Frame = -3

Query: 440 WCKDGAALIGGCCRTTPNTIRAISRSL 360
           W + GA+LIGGCCRTTP+TIRA+S+ L
Sbjct: 291 WQEAGASLIGGCCRTTPSTIRAVSKIL 317



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>HMT1_ARATH (Q9SDL7) Homocysteine S-methyltransferase 1 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 1)
           (SMM:Hcy S-methyltransferase 1) (AtHMT-1)
          Length = 326

 Score = 45.1 bits (105), Expect = 8e-05
 Identities = 20/30 (66%), Positives = 23/30 (76%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSLNQ 354
           +W   GA LIGGCCRTTP+TI AISR L +
Sbjct: 296 KWRDLGAKLIGGCCRTTPSTINAISRDLKR 325



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>HMT2_ARATH (Q9M1W4) Homocysteine S-methyltransferase 2 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 2)
           (SMM:Hcy S-methyltransferase 2) (AtHMT-2)
          Length = 333

 Score = 42.7 bits (99), Expect = 4e-04
 Identities = 17/28 (60%), Positives = 21/28 (75%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSL 360
           +W   G +L+GGCCRTTP TIRAI + L
Sbjct: 300 KWMDAGVSLLGGCCRTTPTTIRAIHKRL 327



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>YM99_YEAST (Q04898) Hypothetical protein YMR321c|
          Length = 105

 Score = 37.7 bits (86), Expect = 0.013
 Identities = 14/31 (45%), Positives = 22/31 (70%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSLNQY 351
           ++   GA +IGGCCRT+P  I+ IS ++ +Y
Sbjct: 74  QYISSGARIIGGCCRTSPKDIQEISAAVKKY 104



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>SAM4_YEAST (Q08985) Homocysteine S-methyltransferase 2 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 2)
           (SMM:Hcy S-methyltransferase 2) (S-adenosylmethionine
           metabolism protein 4)
          Length = 325

 Score = 37.7 bits (86), Expect = 0.013
 Identities = 14/31 (45%), Positives = 22/31 (70%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAISRSLNQY 351
           ++   GA +IGGCCRT+P  I+ IS ++ +Y
Sbjct: 294 QYISSGARIIGGCCRTSPKDIQEISAAVKKY 324



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>MMUM_ECOLI (Q47690) Homocysteine S-methyltransferase (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase)
          Length = 310

 Score = 36.2 bits (82), Expect = 0.037
 Identities = 15/24 (62%), Positives = 17/24 (70%)
 Frame = -3

Query: 443 EWCKDGAALIGGCCRTTPNTIRAI 372
           +W   GA LIGGCCRTTP  I A+
Sbjct: 283 QWQAAGARLIGGCCRTTPADIAAL 306



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>MHT1_YEAST (Q12525) Homocysteine S-methyltransferase 1 (EC 2.1.1.10)|
           (S-methylmethionine:homocysteine methyltransferase 1)
           (SMM:Hcy S-methyltransferase 1)
          Length = 324

 Score = 35.4 bits (80), Expect = 0.063
 Identities = 13/27 (48%), Positives = 21/27 (77%)
 Frame = -3

Query: 431 DGAALIGGCCRTTPNTIRAISRSLNQY 351
           +GA +IGGCCRT+P  I  I+ ++++Y
Sbjct: 297 NGARIIGGCCRTSPKDIAEIASAVDKY 323



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>METH_VIBCH (Q9KUW9) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1226

 Score = 34.3 bits (77), Expect = 0.14
 Identities = 16/36 (44%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAISRSLNQYYPAA 339
           EW + G   L+GGCC TTP  I AI++++    P A
Sbjct: 298 EWAQAGFLNLVGGCCGTTPEHIAAIAKAVEGVKPRA 333



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>METH_VIBPA (Q87L95) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1226

 Score = 33.9 bits (76), Expect = 0.18
 Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAISRSLNQYYPAA 339
           EW   G   LIGGCC TTP  IR +++++    P A
Sbjct: 298 EWASSGFLNLIGGCCGTTPEHIRQMAQAVEGVTPRA 333



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>METH_ECOLI (P13009) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase,
           vitamin-B12-dependent isozyme) (MS)
          Length = 1226

 Score = 33.5 bits (75), Expect = 0.24
 Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAISRSLNQYYP 345
           EW + G   ++GGCC TTP  I A+SR++    P
Sbjct: 296 EWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAP 329



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>METH_SALTY (P37586) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1226

 Score = 33.1 bits (74), Expect = 0.31
 Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAISRSLNQYYP 345
           EW + G   ++GGCC TTP  I A+SR++    P
Sbjct: 296 EWAEAGFLNIVGGCCGTTPEHIAAMSRAVAGLLP 329



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>METH_PSEPU (O33465) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS) (Fragment)
          Length = 607

 Score = 32.3 bits (72), Expect = 0.53
 Identities = 15/37 (40%), Positives = 23/37 (62%)
 Frame = -3

Query: 419 LIGGCCRTTPNTIRAISRSLNQYYPAA*VFAAQSCTL 309
           +IGGC  TTP  I+AI+ ++ +Y P      A++C L
Sbjct: 309 IIGGCSGTTPGHIQAIAEAVAKYKPREIPEIAKACRL 345



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>METH_VIBVU (Q8DCJ7) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1226

 Score = 32.0 bits (71), Expect = 0.70
 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAISRSLNQYYP 345
           EW + G   LIGGCC TTP  IR ++ ++    P
Sbjct: 298 EWAQSGFLNLIGGCCGTTPEHIRHMAMAVEGVSP 331



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>METH_VIBVY (Q7MHB1) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1226

 Score = 31.6 bits (70), Expect = 0.91
 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAISRSLNQYYP 345
           EW + G   LIGGCC TTP  IR ++ ++    P
Sbjct: 298 EWAQSGFLNLIGGCCGTTPEHIRHMAMAVEGESP 331



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>METH_MYCLE (Q49775) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1206

 Score = 31.6 bits (70), Expect = 0.91
 Identities = 11/23 (47%), Positives = 18/23 (78%)
 Frame = -3

Query: 428 GAALIGGCCRTTPNTIRAISRSL 360
           G +L+GGCC TTP+ IR ++ ++
Sbjct: 292 GLSLVGGCCGTTPDHIREVAAAV 314



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>METH_CAEEL (Q09582) Probable methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent) (MS)
          Length = 1249

 Score = 31.6 bits (70), Expect = 0.91
 Identities = 14/34 (41%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAISRSLNQYYP 345
           E+ +DG   +IGGCC TTP+ I A+ +++    P
Sbjct: 299 EFARDGLVNIIGGCCGTTPDHINAMYKAVQGITP 332



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>METH_VIBFI (Q9AJQ8) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1226

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 14/26 (53%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAIS 369
           EW   G   LIGGCC TTP  IR ++
Sbjct: 299 EWASSGFLNLIGGCCGTTPEHIRQMA 324



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>METH_VIBF1 (Q5E814) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1226

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 14/26 (53%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAIS 369
           EW   G   LIGGCC TTP  IR ++
Sbjct: 299 EWASSGFLNLIGGCCGTTPEHIRQMA 324



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>METH_MYCTU (O33259) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent isozyme) (MS)
          Length = 1192

 Score = 30.4 bits (67), Expect = 2.0
 Identities = 11/23 (47%), Positives = 17/23 (73%)
 Frame = -3

Query: 428 GAALIGGCCRTTPNTIRAISRSL 360
           G +L+GGCC TTP  IR ++ ++
Sbjct: 290 GLSLVGGCCGTTPAHIREVAAAV 312



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>ARLY_PROMM (Q7TV86) Argininosuccinate lyase (EC 4.3.2.1) (Arginosuccinase)|
           (ASAL)
          Length = 470

 Score = 29.3 bits (64), Expect = 4.5
 Identities = 14/45 (31%), Positives = 23/45 (51%)
 Frame = +3

Query: 192 RRFANVSAQLSHFAGERHAAITKSPGHVGNITPGVTSLKEGTTLC 326
           RR  ++  +L  F   +HA +T++  H   + PG T L+    LC
Sbjct: 131 RRLDDLDCELERF---QHALLTQAESHRQTLIPGYTHLQRAQPLC 172



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>METH_HUMAN (Q99707) Methionine synthase (EC 2.1.1.13)|
           (5-methyltetrahydrofolate--homocysteine
           methyltransferase) (Methionine synthase, vitamin-B12
           dependent) (MS)
          Length = 1265

 Score = 29.3 bits (64), Expect = 4.5
 Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = -3

Query: 443 EWCKDGAA-LIGGCCRTTPNTIRAISRSLNQYYP 345
           ++  DG   ++GGCC +TP+ IR I+ ++    P
Sbjct: 310 DFAMDGLVNIVGGCCGSTPDHIREIAEAVKNCKP 343



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>NUD10_ARATH (Q6NPD7) Nudix hydrolase 10 (EC 3.6.1.-) (AtNUDT10) (ADP-ribose|
           pyrophosphatase) (EC 3.6.1.13) (NADH pyrophosphatase)
           (EC 3.6.1.22)
          Length = 277

 Score = 28.5 bits (62), Expect = 7.7
 Identities = 10/26 (38%), Positives = 13/26 (50%)
 Frame = +3

Query: 360 KRSTYCSDGVWSCPAAAPDKGSPIFA 437
           K  + C  G+W  P    D+G  IFA
Sbjct: 127 KYGSLCGSGIWKIPTGVVDEGEEIFA 152


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,386,568
Number of Sequences: 219361
Number of extensions: 1127766
Number of successful extensions: 2696
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 2616
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2695
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2618960580
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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