ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart53h06
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1POT1_ARATH (O22397) Potassium transporter 1 (AtPOT1) (AtKUP1) (A... 30 1.5
2UBP35_HUMAN (Q9P2H5) Ubiquitin carboxyl-terminal hydrolase 35 (E... 30 1.5
3IFH1_YEAST (P39520) Protein IFH1 (Protein RRP3) 30 1.9
4HS90B_RAT (P34058) Heat shock protein HSP 90-beta (HSP 84) 30 1.9
5HS90B_MOUSE (P11499) Heat shock protein HSP 90-beta (HSP 84) (Tu... 30 1.9
6HS90B_HUMAN (P08238) Heat shock protein HSP 90-beta (HSP 84) (HS... 30 1.9
7HS90B_HORSE (Q9GKX8) Heat shock protein HSP 90-beta (HSP 84) 30 1.9
8TRI34_HUMAN (Q9BYJ4) Tripartite motif protein 34 (Interferon-res... 29 2.5
9SPB42_CANAL (Q59NP8) ATP-dependent rRNA helicase SPB42 (EC 3.6.1.-) 29 3.3
10SFRS4_HUMAN (Q08170) Splicing factor, arginine/serine-rich 4 (Pr... 29 3.3
11YFI6_YEAST (P43597) Hypothetical 137.7 kDa protein in UGS1-FAB1 ... 28 4.3
12MYLK_HUMAN (Q15746) Myosin light chain kinase, smooth muscle and... 28 4.3
13MYLK_RABIT (P29294) Myosin light chain kinase, smooth muscle (EC... 28 4.3
14DNAA_STRAW (Q82FD8) Chromosomal replication initiator protein dnaA 28 5.6
15YJN1_YEAST (P47015) Hypothetical 41.5 kDa protein in MRS3-URA2 i... 28 5.6
16DNL1_HUMAN (P18858) DNA ligase 1 (EC 6.5.1.1) (DNA ligase I) (Po... 28 5.6
17DNJCC_RAT (Q925T0) DnaJ homolog subfamily C member 12 (J domain-... 27 9.5
18CTL4_RAT (Q6MG71) Choline transporter-like protein 4 (Solute car... 27 9.5
19PTHR_RABIT (Q9GLC7) Parathyroid hormone-related protein precurso... 27 9.5

>POT1_ARATH (O22397) Potassium transporter 1 (AtPOT1) (AtKUP1) (AtKT1)|
          Length = 712

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 15/25 (60%), Positives = 18/25 (72%)
 Frame = +2

Query: 65  EPQKEEEETTSGKRKKEKTDEIQEA 139
           EP  EEEE +S +RKKE+  EI EA
Sbjct: 626 EPGLEEEEMSSVRRKKEECMEIMEA 650



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>UBP35_HUMAN (Q9P2H5) Ubiquitin carboxyl-terminal hydrolase 35 (EC 3.1.2.15)|
           (Ubiquitin thioesterase 35)
           (Ubiquitin-specific-processing protease 35)
           (Deubiquitinating enzyme 35)
          Length = 1017

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 13/29 (44%), Positives = 21/29 (72%)
 Frame = +2

Query: 50  KRTAIEPQKEEEETTSGKRKKEKTDEIQE 136
           +RT  E   EEEE+T G+ ++EK +E++E
Sbjct: 685 ERTEKEEVGEEEESTRGEGEREKEEEVEE 713



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>IFH1_YEAST (P39520) Protein IFH1 (Protein RRP3)|
          Length = 1085

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 13/47 (27%), Positives = 27/47 (57%)
 Frame = +2

Query: 17  LFFNQSRSILPKRTAIEPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ 157
           L+ ++  S   K   + P+KE EE    +++KEK ++ ++   N+K+
Sbjct: 192 LYSSRENSNKNKSVKLSPKKENEEEQKEEKEKEKEEQQKQQESNKKE 238



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>HS90B_RAT (P34058) Heat shock protein HSP 90-beta (HSP 84)|
          Length = 723

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = +2

Query: 50  KRTAIEPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ*ISCLDKWT 184
           ++  IE    +EE  SGK KK+KT +I+E   +Q++       WT
Sbjct: 245 EKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWT 289



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>HS90B_MOUSE (P11499) Heat shock protein HSP 90-beta (HSP 84) (Tumor-specific|
           transplantation 84 kDa antigen) (TSTA)
          Length = 723

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = +2

Query: 50  KRTAIEPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ*ISCLDKWT 184
           ++  IE    +EE  SGK KK+KT +I+E   +Q++       WT
Sbjct: 245 EKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWT 289



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>HS90B_HUMAN (P08238) Heat shock protein HSP 90-beta (HSP 84) (HSP 90)|
          Length = 723

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = +2

Query: 50  KRTAIEPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ*ISCLDKWT 184
           ++  IE    +EE  SGK KK+KT +I+E   +Q++       WT
Sbjct: 245 EKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWT 289



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>HS90B_HORSE (Q9GKX8) Heat shock protein HSP 90-beta (HSP 84)|
          Length = 723

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = +2

Query: 50  KRTAIEPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ*ISCLDKWT 184
           ++  IE    +EE  SGK KK+KT +I+E   +Q++       WT
Sbjct: 245 EKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWT 289



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>TRI34_HUMAN (Q9BYJ4) Tripartite motif protein 34 (Interferon-responsive finger|
           protein 1) (RING finger protein 21)
          Length = 488

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 21/70 (30%), Positives = 33/70 (47%), Gaps = 7/70 (10%)
 Frame = +2

Query: 23  FNQSRSILPKRTAIEPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ-------*ISCLDKW 181
           F+Q RSIL      E Q+ EEE    K+  +K  E ++ +  QKQ        + C  +W
Sbjct: 187 FDQLRSILNNEEQRELQRLEEEE---KKTLDKFAEAEDELVQQKQLVRELISDVECRSQW 243

Query: 182 TCMHMVDRWS 211
           + M ++   S
Sbjct: 244 STMELLQDMS 253



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>SPB42_CANAL (Q59NP8) ATP-dependent rRNA helicase SPB42 (EC 3.6.1.-)|
          Length = 631

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 14/29 (48%), Positives = 19/29 (65%)
 Frame = +2

Query: 50  KRTAIEPQKEEEETTSGKRKKEKTDEIQE 136
           KR AIE Q  EEE ++G   K++ DE+ E
Sbjct: 577 KREAIEKQIMEEERSNGGGNKDEEDEVVE 605



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>SFRS4_HUMAN (Q08170) Splicing factor, arginine/serine-rich 4 (Pre-mRNA-splicing|
           factor SRP75) (SRP001LB)
          Length = 494

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
 Frame = +2

Query: 26  NQSRSILPKRTAIEPQKEEEETTS---GKRKKEKTDEIQEAIQN 148
           +QSRS   K  +  P KE+  + S   GK + +  D+ +E IQN
Sbjct: 236 SQSRSRSKKEKSRSPSKEKSRSRSHSAGKSRSKSKDQAEEKIQN 279



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>YFI6_YEAST (P43597) Hypothetical 137.7 kDa protein in UGS1-FAB1 intergenic|
           region
          Length = 1233

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +2

Query: 65  EPQKEEEETTSGKRKKEKTDEIQEAIQNQK 154
           E ++EEE +T  K KKE     QEA++N +
Sbjct: 471 EEEEEEENSTFSKVKKENVTGEQEAVRNNE 500



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>MYLK_HUMAN (Q15746) Myosin light chain kinase, smooth muscle and non-muscle|
            isozymes (EC 2.7.11.18) (MLCK) [Contains: Telokin
            (Kinase-related protein) (KRP)]
          Length = 1914

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 11/31 (35%), Positives = 21/31 (67%)
 Frame = +2

Query: 65   EPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ 157
            EP +E E TT G++ +E  DE++ +  ++K+
Sbjct: 1414 EPSQESELTTVGEKPEEPKDEVEVSDDDEKE 1444



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>MYLK_RABIT (P29294) Myosin light chain kinase, smooth muscle (EC 2.7.11.18)|
           (MLCK) [Contains: Telokin]
          Length = 1147

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 11/31 (35%), Positives = 20/31 (64%)
 Frame = +2

Query: 65  EPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ 157
           EP +E E TT G++ +E  DE++E   + ++
Sbjct: 645 EPSQESELTTVGEKPEEPKDEVEEVSDDDEK 675



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>DNAA_STRAW (Q82FD8) Chromosomal replication initiator protein dnaA|
          Length = 653

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 18/62 (29%), Positives = 30/62 (48%)
 Frame = +1

Query: 70  PKGRGRNYVGQKKKREDG*DSGSYPKPKAMNFLSGQVDMHAHG*SMELEPNRIDQMSTAQ 249
           P G G+     + +  +G +   Y + +A +   G  D H  G   +L P+R DQ+ TA+
Sbjct: 112 PSGPGQGRDPYESRGREGYEG--YGRHRADDHRQGHNDRHQGGPGDQLPPSRGDQLPTAR 169

Query: 250 AA 255
            A
Sbjct: 170 PA 171



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>YJN1_YEAST (P47015) Hypothetical 41.5 kDa protein in MRS3-URA2 intergenic|
           region
          Length = 356

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 12/38 (31%), Positives = 24/38 (63%)
 Frame = +2

Query: 41  ILPKRTAIEPQKEEEETTSGKRKKEKTDEIQEAIQNQK 154
           ++PK   ++PQ  ++      RKKE+ +++Q+ IQ +K
Sbjct: 317 LIPKD--VKPQNNDKRALKELRKKERQEKLQKRIQRKK 352



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>DNL1_HUMAN (P18858) DNA ligase 1 (EC 6.5.1.1) (DNA ligase I)|
           (Polydeoxyribonucleotide synthase [ATP] 1)
          Length = 919

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
 Frame = +2

Query: 32  SRSILPKRTAIEPQKE--EEETTSGKRKKEKTDE 127
           +R  LPKRT  E  +E  E+E    KRKKE+ +E
Sbjct: 124 ARKQLPKRTIQEVLEEQSEDEDREAKRKKEEEEE 157



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>DNJCC_RAT (Q925T0) DnaJ homolog subfamily C member 12 (J domain-containing|
           protein 1)
          Length = 198

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 12/49 (24%), Positives = 25/49 (51%)
 Frame = +2

Query: 65  EPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ*ISCLDKWTCMHMVDRWS 211
           E ++ + ++T  K  +++++  ++ I  Q      L  W C H+  RWS
Sbjct: 134 ETKQGDPDSTPEKMMQKESESPEKGISPQNPDSPGLSDWNCGHLHFRWS 182



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>CTL4_RAT (Q6MG71) Choline transporter-like protein 4 (Solute carrier family|
           44 member 4)
          Length = 707

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 14/44 (31%), Positives = 21/44 (47%)
 Frame = -1

Query: 275 YFRFQQAAACAVDI*SIRFGSSSIDQPCACMSTCPDKKFIAFGF 144
           YF     AA A+++ SI         P  C+S+CP   ++  GF
Sbjct: 86  YFNILSCAA-AINVISIAENGLQCPTPQVCVSSCPQTPWVVEGF 128



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>PTHR_RABIT (Q9GLC7) Parathyroid hormone-related protein precursor (PTH-rP)|
           (PTHrP) [Contains: Osteostatin]
          Length = 177

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 12/44 (27%), Positives = 25/44 (56%)
 Frame = +2

Query: 26  NQSRSILPKRTAIEPQKEEEETTSGKRKKEKTDEIQEAIQNQKQ 157
           ++ R +  +   +EP KE+   T GK+KK K  + +E  + +++
Sbjct: 99  DEGRYLTQETNKVEPYKEQPLKTPGKKKKGKPGKRKEQEKKKRR 142


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,740,568
Number of Sequences: 219361
Number of extensions: 492894
Number of successful extensions: 2260
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 2093
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2233
length of database: 80,573,946
effective HSP length: 91
effective length of database: 60,612,095
effective search space used: 1454690280
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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