ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart53e05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1OPR1_LYCES (Q9XG54) 12-oxophytodienoate reductase 1 (EC 1.3.1.42... 112 3e-25
2OPR1_ARATH (Q8LAH7) 12-oxophytodienoate reductase 1 (EC 1.3.1.42... 110 6e-25
3OPR2_ARATH (Q8GYB8) 12-oxophytodienoate reductase 2 (EC 1.3.1.42... 109 2e-24
4OPRL_LYCES (Q9FEX0) 12-oxophytodienoate reductase-like protein (... 108 3e-24
5OPR3_LYCES (Q9FEW9) 12-oxophytodienoate reductase 3 (EC 1.3.1.42... 102 2e-22
6OPR3_ARATH (Q9FUP0) 12-oxophytodienoate reductase 3 (EC 1.3.1.42... 96 2e-20
7OPRL1_ARATH (Q8GYA3) Putative 12-oxophytodienoate reductase-like... 75 3e-14
8OYEB_SCHPO (Q09671) Putative NADPH dehydrogenase C5H10.10 (EC 1.... 66 2e-11
9OYEA_SCHPO (Q09670) Putative NADPH dehydrogenase C5H10.04 (EC 1.... 59 2e-09
10OYE3_YEAST (P41816) NADPH dehydrogenase 3 (EC 1.6.99.1) (Old yel... 57 8e-09
11OYE1_SACPS (Q02899) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yel... 54 7e-08
12OYE2_YEAST (Q03558) NADPH dehydrogenase 2 (EC 1.6.99.1) (Old yel... 54 1e-07
13KYE1_KLULA (P40952) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yel... 51 8e-07
14NEMA_ECOLI (P77258) N-ethylmaleimide reductase (EC 1.-.-.-) (N-e... 47 1e-05
15EBP1_CANAL (P43084) Probable NADPH dehydrogenase (EC 1.6.99.1) (... 39 0.004
16NADO_THEBR (P32382) NADH oxidase (EC 1.-.-.-) 33 0.22
17Y435_BUCAI (P57510) Hypothetical protein BU435 30 1.4
18MAD1_YEAST (P40957) Spindle assembly checkpoint component MAD1 (... 30 1.4
19ISPH_CLOTE (Q895G2) 4-hydroxy-3-methylbut-2-enyl diphosphate red... 29 2.4
20G3P_AERPE (Q9YFS9) Glyceraldehyde-3-phosphate dehydrogenase (EC ... 29 2.4
21DCNL2_MOUSE (Q8BZJ7) DCN1-like protein 2 (Defective in cullin ne... 29 3.1
22DLTC_LACRH (P55153) D-alanine--poly(phosphoribitol) ligase subun... 28 4.1
23Y31K_SSV1 (P20203) Hypothetical 31.5 kDa protein (ORF B-277) 28 4.1
24XIST_MOUSE (P27571) X inactive-specific transcript protein (Frag... 28 5.4
25KCNA2_XENLA (P22739) Potassium voltage-gated channel subfamily A... 28 5.4
26DCNL1_MOUSE (Q9QZ73) DCN1-like protein 1 (Defective in cullin ne... 27 9.1

>OPR1_LYCES (Q9XG54) 12-oxophytodienoate reductase 1 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 1) (OPDA-reductase
           1) (LeOPR1)
          Length = 376

 Score =  112 bits (279), Expect = 3e-25
 Identities = 47/66 (71%), Positives = 60/66 (90%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           GGYDR++GN+ + +  ADLVAYGR+F++NPDLPKRF L+APLNKY+R+TFYT DPI+GYT
Sbjct: 308 GGYDREDGNRALIEDRADLVAYGRLFISNPDLPKRFELNAPLNKYNRDTFYTSDPIVGYT 367

Query: 202 DYPFLD 185
           DYPFL+
Sbjct: 368 DYPFLE 373



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>OPR1_ARATH (Q8LAH7) 12-oxophytodienoate reductase 1 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 1) (OPDA-reductase
           1) (AtOPR1) (FS-AT-I)
          Length = 372

 Score =  110 bits (276), Expect = 6e-25
 Identities = 48/68 (70%), Positives = 58/68 (85%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           GG+ R++GN+ V+ G  DLVAYGR FLANPDLPKRF +DAPLNKYDR TFYT DP++GYT
Sbjct: 304 GGFTREDGNEAVSKGRTDLVAYGRWFLANPDLPKRFQVDAPLNKYDRPTFYTSDPVVGYT 363

Query: 202 DYPFLDGS 179
           DYPFL+ +
Sbjct: 364 DYPFLEST 371



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>OPR2_ARATH (Q8GYB8) 12-oxophytodienoate reductase 2 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 2) (OPDA-reductase
           2) (AtOPR2)
          Length = 374

 Score =  109 bits (272), Expect = 2e-24
 Identities = 48/68 (70%), Positives = 58/68 (85%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           GG+ R++GN+ VA G  DLVAYGR FLANPDLPKRF LDAPLNKY+R+TFYT DP++GYT
Sbjct: 306 GGFTREDGNEAVAKGRTDLVAYGRWFLANPDLPKRFQLDAPLNKYNRSTFYTSDPVVGYT 365

Query: 202 DYPFLDGS 179
           DYP L+ +
Sbjct: 366 DYPSLEST 373



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>OPRL_LYCES (Q9FEX0) 12-oxophytodienoate reductase-like protein (EC 1.3.1.-)|
           (LeOPR2)
          Length = 355

 Score =  108 bits (270), Expect = 3e-24
 Identities = 45/66 (68%), Positives = 58/66 (87%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           GGY + +G K + + YADL+++GR+FLANPDLPKRF ++APLNKY+R+TFYT DPIIGYT
Sbjct: 287 GGYGKSDGEKAIDENYADLISFGRMFLANPDLPKRFEVNAPLNKYNRSTFYTNDPIIGYT 346

Query: 202 DYPFLD 185
           DYPFL+
Sbjct: 347 DYPFLE 352



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>OPR3_LYCES (Q9FEW9) 12-oxophytodienoate reductase 3 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 3) (OPDA-reductase
           3) (LeOPR3)
          Length = 396

 Score =  102 bits (254), Expect = 2e-22
 Identities = 45/70 (64%), Positives = 57/70 (81%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           GGY R+ G + VA G ADLV+YGR+F++NPDL  R  L+APLNKY+R TFYTQDP++GYT
Sbjct: 320 GGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNKYNRKTFYTQDPVVGYT 379

Query: 202 DYPFLDGSNA 173
           DYPFL G+ +
Sbjct: 380 DYPFLQGNGS 389



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>OPR3_ARATH (Q9FUP0) 12-oxophytodienoate reductase 3 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 3) (OPDA-reductase
           3) (Delayed dehiscence 1) (AtOPR3)
          Length = 391

 Score = 96.3 bits (238), Expect = 2e-20
 Identities = 41/65 (63%), Positives = 53/65 (81%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           GG++++ G + V  G ADLV+YGR+F+ANPDL  RF +D  LNKY+R TFYTQDP++GYT
Sbjct: 321 GGFNKELGMQAVQQGDADLVSYGRLFIANPDLVSRFKIDGELNKYNRKTFYTQDPVVGYT 380

Query: 202 DYPFL 188
           DYPFL
Sbjct: 381 DYPFL 385



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>OPRL1_ARATH (Q8GYA3) Putative 12-oxophytodienoate reductase-like protein 1 (EC|
           1.3.1.-)
          Length = 324

 Score = 75.5 bits (184), Expect = 3e-14
 Identities = 34/44 (77%), Positives = 39/44 (88%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNK 251
           GGY R++GNK VA+G  DLVAYGR+FLANPDLPKRF L+APLNK
Sbjct: 281 GGYTREDGNKAVAEGRTDLVAYGRLFLANPDLPKRFELNAPLNK 324



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>OYEB_SCHPO (Q09671) Putative NADPH dehydrogenase C5H10.10 (EC 1.6.99.1) (Old|
           yellow enzyme homolog)
          Length = 392

 Score = 66.2 bits (160), Expect = 2e-11
 Identities = 34/64 (53%), Positives = 43/64 (67%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           GG+DR    ++ A+    LVA+GR FL+NPDLP R   + PLNK+DR TFYT+    GY 
Sbjct: 319 GGHDRDSAIQM-AEQENTLVAFGRYFLSNPDLPFRLKYNLPLNKWDRATFYTKMSPKGYI 377

Query: 202 DYPF 191
           DYPF
Sbjct: 378 DYPF 381



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>OYEA_SCHPO (Q09670) Putative NADPH dehydrogenase C5H10.04 (EC 1.6.99.1) (Old|
           yellow enzyme homolog)
          Length = 382

 Score = 59.3 bits (142), Expect = 2e-09
 Identities = 32/64 (50%), Positives = 40/64 (62%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           GGYD +   +  A+    LVA+GR F+ANPDL  R     PLNK+DR++FY      GYT
Sbjct: 312 GGYDPETAVQA-ANERGVLVAFGRNFIANPDLVFRIKHHIPLNKWDRSSFYLPKTEKGYT 370

Query: 202 DYPF 191
           DYPF
Sbjct: 371 DYPF 374



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>OYE3_YEAST (P41816) NADPH dehydrogenase 3 (EC 1.6.99.1) (Old yellow enzyme 3)|
          Length = 399

 Score = 57.4 bits (137), Expect = 8e-09
 Identities = 27/45 (60%), Positives = 31/45 (68%)
 Frame = -2

Query: 328 LVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYTDYP 194
           L+ YGR F++NPDL  R     PLNKYDR+TFYT     GYTDYP
Sbjct: 343 LIGYGRFFISNPDLVYRLEEGLPLNKYDRSTFYTMS-AEGYTDYP 386



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>OYE1_SACPS (Q02899) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yellow enzyme 1)|
          Length = 399

 Score = 54.3 bits (129), Expect = 7e-08
 Identities = 26/45 (57%), Positives = 30/45 (66%)
 Frame = -2

Query: 328 LVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYTDYP 194
           L+ YGR F++NPDL  R     PLNKYDR+TFY Q    GY DYP
Sbjct: 343 LIGYGRFFISNPDLVDRLEKGLPLNKYDRDTFY-QMSAHGYIDYP 386



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>OYE2_YEAST (Q03558) NADPH dehydrogenase 2 (EC 1.6.99.1) (Old yellow enzyme 2)|
          Length = 399

 Score = 53.5 bits (127), Expect = 1e-07
 Identities = 25/45 (55%), Positives = 29/45 (64%)
 Frame = -2

Query: 328 LVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYTDYP 194
           L+ YGR F++NPDL  R     PLNKYDR+TFY      GY DYP
Sbjct: 343 LIGYGRFFISNPDLVDRLEKGLPLNKYDRDTFYKMS-AEGYIDYP 386



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>KYE1_KLULA (P40952) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yellow enzyme 1)|
          Length = 398

 Score = 50.8 bits (120), Expect = 8e-07
 Identities = 24/45 (53%), Positives = 29/45 (64%)
 Frame = -2

Query: 328 LVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYTDYP 194
           L+ YGR F+ANPDL +R     PLN+YDR +FY      GY DYP
Sbjct: 343 LIGYGRAFIANPDLVERLEKGLPLNQYDRPSFYKMS-AEGYIDYP 386



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>NEMA_ECOLI (P77258) N-ethylmaleimide reductase (EC 1.-.-.-) (N-ethylmaleimide|
           reducing enzyme)
          Length = 365

 Score = 46.6 bits (109), Expect = 1e-05
 Identities = 26/65 (40%), Positives = 34/65 (52%)
 Frame = -2

Query: 382 GGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFYTQDPIIGYT 203
           G Y  ++   ++  G  D VA+GR ++ANPDL  R    A LN     +FY      GYT
Sbjct: 302 GAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFY-GGGAEGYT 360

Query: 202 DYPFL 188
           DYP L
Sbjct: 361 DYPTL 365



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>EBP1_CANAL (P43084) Probable NADPH dehydrogenase (EC 1.6.99.1)|
           (Estrogen-binding protein) (EBP)
          Length = 406

 Score = 38.5 bits (88), Expect = 0.004
 Identities = 19/51 (37%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
 Frame = -2

Query: 376 YDRKEGNKVVADGYAD--LVAYGRIFLANPDLPKRFGLDAPLNKYDRNTFY 230
           YD  E   ++ D   D  ++ + R F +NPDL ++  L  PLN Y+R  FY
Sbjct: 333 YDAPEFKTLINDLKNDRSIIGFSRFFTSNPDLVEKLKLGKPLNYYNREEFY 383



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>NADO_THEBR (P32382) NADH oxidase (EC 1.-.-.-)|
          Length = 651

 Score = 32.7 bits (73), Expect = 0.22
 Identities = 13/26 (50%), Positives = 20/26 (76%)
 Frame = -2

Query: 355 KVVADGYADLVAYGRIFLANPDLPKR 278
           K++A+G AD VA GR  +A+P+ PK+
Sbjct: 307 KIIAEGRADFVAVGRGLIADPEWPKK 332



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>Y435_BUCAI (P57510) Hypothetical protein BU435|
          Length = 319

 Score = 30.0 bits (66), Expect = 1.4
 Identities = 13/37 (35%), Positives = 23/37 (62%)
 Frame = +3

Query: 45  YISKLFVEMIHYTCPCIYIPEKKLLQLYKPSKSLTIV 155
           ++S LFV++ + +CP I+   K +L   KPS+   +V
Sbjct: 128 FLSSLFVKIPNQSCPVIHEDNKTILWYEKPSERFLLV 164



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>MAD1_YEAST (P40957) Spindle assembly checkpoint component MAD1 (Mitotic arrest|
           deficient protein 1)
          Length = 749

 Score = 30.0 bits (66), Expect = 1.4
 Identities = 14/43 (32%), Positives = 26/43 (60%)
 Frame = +2

Query: 176 VRAIKERIVCVANDRILCVEGVAVVLVQRSVEPKSLRQIWISK 304
           ++++KER     ND+  C+E +   L  + +E ++LRQ + SK
Sbjct: 124 LKSLKERSANSMNDKDKCIEELRTTLQNKDLEMETLRQQYDSK 166



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>ISPH_CLOTE (Q895G2) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC|
           1.17.1.2)
          Length = 635

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
 Frame = +3

Query: 30  GITWEYISKLF---VEMIHYTCPCIYIPEKKLLQLYKPSKSLTIVEQMNY 170
           G+T E I KL    + +I  TCP +   +KK  + YK   S+ IV   N+
Sbjct: 78  GVTLETIQKLKEKKLNIIDATCPYVSNIQKKAQKYYKEGYSILIVGDKNH 127



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>G3P_AERPE (Q9YFS9) Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.59)|
           (GAPDH) (NAD(P)-dependent glyceraldehyde-3-phosphate
           dehydrogenase)
          Length = 343

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 20/62 (32%), Positives = 32/62 (51%)
 Frame = +2

Query: 194 RIVCVANDRILCVEGVAVVLVQRSVEPKSLRQIWISKKDPSVSNKISIAVGHHFVAFLSI 373
           R++C  N R   VE V  VLV+R  +PK +++      D  V N  ++   HH V   ++
Sbjct: 149 RLICTLN-REFGVESVRAVLVRRGADPKEVKK---GPIDSIVLNPPTLP-SHHAVDVRTV 203

Query: 374 IP 379
           +P
Sbjct: 204 LP 205



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>DCNL2_MOUSE (Q8BZJ7) DCN1-like protein 2 (Defective in cullin neddylation|
           protein 2-like protein 2) (DCUN1 domain-containing
           protein 2)
          Length = 258

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 16/43 (37%), Positives = 23/43 (53%)
 Frame = +2

Query: 254 VQRSVEPKSLRQIWISKKDPSVSNKISIAVGHHFVAFLSIIPS 382
           ++ SV+ K L Q++   KDP   NKI I     F   LS+ P+
Sbjct: 56  MKSSVDQKKLEQLYSRYKDPQDENKIGIDGIQQFCDDLSLDPA 98



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>DLTC_LACRH (P55153) D-alanine--poly(phosphoribitol) ligase subunit 2 (EC|
           6.1.1.13) (D-alanyl carrier protein) (DCP)
          Length = 81

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 11/27 (40%), Positives = 20/27 (74%)
 Frame = -2

Query: 292 DLPKRFGLDAPLNKYDRNTFYTQDPII 212
           +L  +FG+DAP++++DR  + T + II
Sbjct: 48  ELQSQFGVDAPVSEFDRKEWDTPNKII 74



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>Y31K_SSV1 (P20203) Hypothetical 31.5 kDa protein (ORF B-277)|
          Length = 277

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 13/60 (21%), Positives = 31/60 (51%), Gaps = 10/60 (16%)
 Frame = +2

Query: 233 EGVAVVLVQRS----------VEPKSLRQIWISKKDPSVSNKISIAVGHHFVAFLSIIPS 382
           +G++++ ++R+          +E K LR+ W+   +P +  ++    G  FVA   ++P+
Sbjct: 108 DGLSILFLRRAKLIENQGYLMLEVKKLRKAWVLTAEPILLERLKFPFGKKFVAVHFVLPN 167



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>XIST_MOUSE (P27571) X inactive-specific transcript protein (Fragment)|
          Length = 298

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 13/87 (14%)
 Frame = -3

Query: 222 ILSLATQTILSLMARTLNSSSAQL*LMICLVYIIVII----FSPV---------CICTGM 82
           +LS    + +SL    + S+ + L + +C +Y++ I     F+P          C+   +
Sbjct: 112 VLSCRALSFVSLSLVPVWSALSSLAMALCFIYLVAISLSFSFTPAFRDSLWVTCCMHPSL 171

Query: 81  CSVSFPQTIYLCTPM*CQYVKIFFKLI 1
             V  P  + LC+   CQ + +FF L+
Sbjct: 172 FLVLTP--LVLCSSSLCQSISVFFLLL 196



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>KCNA2_XENLA (P22739) Potassium voltage-gated channel subfamily A member 2|
           (Voltage-gated potassium channel subunit Kv1.2) (xSHA2)
          Length = 499

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 13/30 (43%), Positives = 22/30 (73%)
 Frame = -3

Query: 195 LSLMARTLNSSSAQL*LMICLVYIIVIIFS 106
           L ++ +TLN+S  +L L+I  ++I VI+FS
Sbjct: 314 LQILGQTLNASMRELGLLIFFLFIGVILFS 343



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>DCNL1_MOUSE (Q9QZ73) DCN1-like protein 1 (Defective in cullin neddylation|
           protein 1-like protein 1) (DCUN1 domain-containing
           protein 1) (Testis-specific protein 3)
          Length = 259

 Score = 27.3 bits (59), Expect = 9.1
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = +2

Query: 254 VQRSVEPKSLRQIWISKKDPSVSNKISIAVGHHFVAFLSIIPS 382
           V+ S++ K L Q++   KDP   NKI I     F   L++ P+
Sbjct: 56  VKGSLDRKKLEQLYTRYKDPQDENKIGIDGIQQFCDDLALDPA 98


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,518,621
Number of Sequences: 219361
Number of extensions: 1136410
Number of successful extensions: 2720
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 2663
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2718
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 1391514312
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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