ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart53c01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MDAR_PEA (Q40977) Monodehydroascorbate reductase (EC 1.6.5.4) (M... 112 3e-25
2MDAR1_ARATH (Q9SR59) Probable monodehydroascorbate reductase, cy... 107 7e-24
3MDAR4_ARATH (Q93WJ8) Probable monodehydroascorbate reductase, cy... 103 1e-22
4MDARS_CUCSA (Q42711) Monodehydroascorbate reductase, seedling is... 94 6e-20
5MDAR_LYCES (Q43497) Monodehydroascorbate reductase (EC 1.6.5.4) ... 92 3e-19
6MDAR3_ARATH (Q9LFA3) Probable monodehydroascorbate reductase, cy... 92 3e-19
7MDAR2_ARATH (Q9LK94) Probable monodehydroascorbate reductase, cy... 56 2e-08
8MDARF_CUCSA (P83966) Monodehydroascorbate reductase, fruit isozy... 54 1e-07
9MDARP_ARATH (P92947) Monodehydroascorbate reductase, chloroplast... 47 9e-06
10ALG14_ASPFU (Q4WNB5) UDP-N-acetylglucosamine transferase subunit... 30 1.1
11K6PP_MOUSE (Q9WUA3) 6-phosphofructokinase type C (EC 2.7.1.11) (... 30 1.1
126PGL_PSEAE (Q9X2N2) 6-phosphogluconolactonase (EC 3.1.1.31) (6PGL) 29 2.4
13CP061_MOUSE (Q8K199) C16orf61 protein homolog 29 3.2
14PRP4B_MOUSE (Q61136) Serine/threonine-protein kinase PRP4 homolo... 28 5.4
15HTPG_RALSO (Q8Y0Q3) Chaperone protein htpG (Heat shock protein h... 28 5.4
16PYRE_PHOPR (Q6LVN7) Orotate phosphoribosyltransferase (EC 2.4.2.... 28 7.0
17NIP29_YEAST (P33419) Protein NIP29 28 7.0
18YIID_ECOLI (P0ADQ2) Hypothetical protein yiiD 28 7.0
19YIID_ECOL6 (P0ADQ3) Hypothetical protein yiiD 28 7.0
20YIID_ECO57 (P0ADQ4) Hypothetical protein yiiD 28 7.0

>MDAR_PEA (Q40977) Monodehydroascorbate reductase (EC 1.6.5.4) (MDAR)|
           (Ascorbate free radical reductase) (AFR reductase)
          Length = 433

 Score =  112 bits (279), Expect = 3e-25
 Identities = 51/68 (75%), Positives = 61/68 (89%)
 Frame = -1

Query: 381 VLFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKE 202
           VLFGDNDPA++KPKFGTYW+K+GKV+G FLEGG+ DEN+AIAKVARA+P V DV  LA+E
Sbjct: 366 VLFGDNDPASSKPKFGTYWIKEGKVVGAFLEGGTPDENKAIAKVARAKPAVEDVNQLAEE 425

Query: 201 GLDFATKI 178
           GL FA+KI
Sbjct: 426 GLSFASKI 433



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>MDAR1_ARATH (Q9SR59) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 1 (EC 1.6.5.4) (MDAR 1)
          Length = 441

 Score =  107 bits (267), Expect = 7e-24
 Identities = 49/67 (73%), Positives = 59/67 (88%)
 Frame = -1

Query: 381 VLFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKE 202
           VLFGDNDP + KPKFGTYWVKDGKV+GVFLEGG+ +E++AIAKVARAQP V  ++ L++E
Sbjct: 369 VLFGDNDPKSPKPKFGTYWVKDGKVVGVFLEGGTQEEHKAIAKVARAQPSVESLDVLSEE 428

Query: 201 GLDFATK 181
           GL FATK
Sbjct: 429 GLSFATK 435



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>MDAR4_ARATH (Q93WJ8) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 4 (EC 1.6.5.4) (MDAR 4)
          Length = 435

 Score =  103 bits (256), Expect = 1e-22
 Identities = 47/68 (69%), Positives = 56/68 (82%)
 Frame = -1

Query: 381 VLFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKE 202
           VLFGDNDP + KPKFG+YW+K+ KV+G FLEGGS +EN AIAK+ARAQP V  +E L+KE
Sbjct: 368 VLFGDNDPESPKPKFGSYWIKERKVVGAFLEGGSPEENNAIAKLARAQPSVESLEVLSKE 427

Query: 201 GLDFATKI 178
           GL FAT I
Sbjct: 428 GLSFATNI 435



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>MDARS_CUCSA (Q42711) Monodehydroascorbate reductase, seedling isozyme (EC|
           1.6.5.4) (MDAR seedling) (Ascorbate free radical
           reductase seedling) (AFR reductase seedling)
          Length = 434

 Score = 94.4 bits (233), Expect = 6e-20
 Identities = 43/68 (63%), Positives = 53/68 (77%)
 Frame = -1

Query: 381 VLFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKE 202
           VLFGDN P +A  KFG+YW+KDGKV+G FLE GS +EN+AIAKVAR QP V   + L KE
Sbjct: 367 VLFGDNSPDSATHKFGSYWIKDGKVVGAFLESGSPEENKAIAKVARIQPSVESSDLLLKE 426

Query: 201 GLDFATKI 178
           G+ FA+K+
Sbjct: 427 GISFASKV 434



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>MDAR_LYCES (Q43497) Monodehydroascorbate reductase (EC 1.6.5.4) (MDAR)|
           (Ascorbate free radical reductase) (AFR reductase)
          Length = 433

 Score = 92.0 bits (227), Expect = 3e-19
 Identities = 43/68 (63%), Positives = 55/68 (80%)
 Frame = -1

Query: 381 VLFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKE 202
           VLFGD DP +A  KFG YW+KDGK++G FLE GS +EN+AIAKVA+ QPP A ++ LA+E
Sbjct: 367 VLFGDADPNSATHKFGQYWIKDGKIVGAFLESGSPEENKAIAKVAKVQPP-ATLDQLAQE 425

Query: 201 GLDFATKI 178
           G+ FA+KI
Sbjct: 426 GISFASKI 433



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>MDAR3_ARATH (Q9LFA3) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 3 (EC 1.6.5.4) (MDAR 3)
          Length = 434

 Score = 92.0 bits (227), Expect = 3e-19
 Identities = 39/68 (57%), Positives = 54/68 (79%)
 Frame = -1

Query: 381 VLFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKE 202
           VLFGD++P+  KP+FG YWV+ GKV+G F+EGGS DEN+A+AKVA+A+P    ++ L K+
Sbjct: 367 VLFGDSNPSNPKPRFGAYWVQGGKVVGAFMEGGSGDENKALAKVAKARPSAESLDELVKQ 426

Query: 201 GLDFATKI 178
           G+ FA KI
Sbjct: 427 GISFAAKI 434



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>MDAR2_ARATH (Q9LK94) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 2 (EC 1.6.5.4) (MDAR 2)
          Length = 488

 Score = 56.2 bits (134), Expect = 2e-08
 Identities = 26/52 (50%), Positives = 36/52 (69%), Gaps = 1/52 (1%)
 Frame = -1

Query: 339 FGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVA-DVEALAKEGLDFA 187
           FG YWVK G ++G FLEGG+ +E + I+K  + +P V  D+E L +EGL FA
Sbjct: 374 FGAYWVKKGHLVGSFLEGGTKEEYETISKATQLKPAVTIDLEELEREGLGFA 425



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>MDARF_CUCSA (P83966) Monodehydroascorbate reductase, fruit isozyme (EC 1.6.5.4)|
           (MDAR fruit) (Ascorbate free radical reductase fruit)
           (AFR reductase fruit) (Fragments)
          Length = 166

 Score = 53.5 bits (127), Expect = 1e-07
 Identities = 32/59 (54%), Positives = 37/59 (62%)
 Frame = -1

Query: 381 VLFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAK 205
           VLF DN        FGTYW+K   V+GVFLEGG+ DE     KVAR QPPV  ++ LAK
Sbjct: 123 VLFPDN--------FGTYWIK---VVGVFLEGGTPDE----YKVARVQPPVESLDQLAK 166



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>MDARP_ARATH (P92947) Monodehydroascorbate reductase, chloroplast precursor (EC|
           1.6.5.4) (MDAR)
          Length = 493

 Score = 47.4 bits (111), Expect = 9e-06
 Identities = 24/71 (33%), Positives = 40/71 (56%), Gaps = 5/71 (7%)
 Frame = -1

Query: 375 FGDNDPAAAK-----PKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEAL 211
           FGDN     +     PK  T+W++ G++ GV +E GS +E Q + K+AR+QP V   +  
Sbjct: 416 FGDNVGETVEVGNFDPKIATFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLA 475

Query: 210 AKEGLDFATKI 178
           +   ++ A +I
Sbjct: 476 SASSVEEALEI 486



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>ALG14_ASPFU (Q4WNB5) UDP-N-acetylglucosamine transferase subunit alg14 (EC|
           2.4.1.-) (Asparagine linked glycosylation protein 14)
          Length = 228

 Score = 30.4 bits (67), Expect = 1.1
 Identities = 15/45 (33%), Positives = 23/45 (51%)
 Frame = -1

Query: 372 GDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQ 238
           GDN  AA   +F T W+K    +     G ++ E+ A+  V RA+
Sbjct: 25  GDNFSAARAVEFETEWLKQSPKLSFPANGSNSTESYAVVTVPRAR 69



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>K6PP_MOUSE (Q9WUA3) 6-phosphofructokinase type C (EC 2.7.1.11)|
           (Phosphofructokinase 1) (Phosphohexokinase)
           (Phosphofructo-1-kinase isozyme C) (PFK-C)
          Length = 784

 Score = 30.4 bits (67), Expect = 1.1
 Identities = 17/43 (39%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
 Frame = -1

Query: 369 DNDPAAAKPKFGTYWVKDGKVIGVFLEGGSAD-ENQAIAKVAR 244
           D+  ++A PK+  +   DGK IGV   GG A   N A+  V R
Sbjct: 5   DSSSSSAYPKYLEHLSGDGKAIGVLTSGGDAQGMNAAVRAVVR 47



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>6PGL_PSEAE (Q9X2N2) 6-phosphogluconolactonase (EC 3.1.1.31) (6PGL)|
          Length = 238

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 14/36 (38%), Positives = 24/36 (66%)
 Frame = -1

Query: 294 LEGGSADENQAIAKVARAQPPVADVEALAKEGLDFA 187
           L    A++ QA+  V+  + PVA +EAL++E LD++
Sbjct: 35  LRSALAEQGQALLVVSGGRSPVAFLEALSEEPLDWS 70



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>CP061_MOUSE (Q8K199) C16orf61 protein homolog|
          Length = 79

 Score = 28.9 bits (63), Expect = 3.2
 Identities = 16/46 (34%), Positives = 19/46 (41%), Gaps = 8/46 (17%)
 Frame = +1

Query: 13  LNLLKSCHS--------GHSNTIKLQKEKKKSNHVSELGTRKHRQG 126
           +NLLK CH         GH N +  +  K   N  SE  TR    G
Sbjct: 18  INLLKECHKNHNILKFFGHCNDLDREMRKCLKNEYSERRTRSREHG 63



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>PRP4B_MOUSE (Q61136) Serine/threonine-protein kinase PRP4 homolog (EC 2.7.11.1)|
           (PRP4 pre-mRNA-processing factor 4 homolog) (Pre-mRNA
           protein kinase)
          Length = 1007

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
 Frame = +1

Query: 34  HSGHSNTIKLQKEK-KKSNHVSELGTRKHRQGRDKHKIYTRR*IIEES 174
           HS H       + K KK  H SE    K  + + KHK + R+ +IE S
Sbjct: 41  HSRHKKKKHKHRSKHKKHKHSSEEDRDKKHKHKHKHKKHKRKEVIEAS 88



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>HTPG_RALSO (Q8Y0Q3) Chaperone protein htpG (Heat shock protein htpG) (High|
           temperature protein G)
          Length = 640

 Score = 28.1 bits (61), Expect = 5.4
 Identities = 16/42 (38%), Positives = 26/42 (61%)
 Frame = -1

Query: 369 DNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADENQAIAKVAR 244
           D+D AA K K+ T+W   G+V+   L    A++++ IAK+ R
Sbjct: 370 DSDDAAEKDKYATFWQHFGQVLKEGLGEDFANKDR-IAKLLR 410



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>PYRE_PHOPR (Q6LVN7) Orotate phosphoribosyltransferase (EC 2.4.2.10) (OPRT)|
           (OPRTase)
          Length = 213

 Score = 27.7 bits (60), Expect = 7.0
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = -1

Query: 342 KFGTYWVKDGKVIGVFLEGGSADENQAIAKVARAQPPVADVEALAKEGLDF 190
           KFG + +K G+V   F   G  +  + +A++ R        EAL   G+D+
Sbjct: 19  KFGEFTLKSGRVSPYFFNAGLFNTGRDLARLGRFY-----AEALVDAGIDY 64



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>NIP29_YEAST (P33419) Protein NIP29|
          Length = 253

 Score = 27.7 bits (60), Expect = 7.0
 Identities = 10/31 (32%), Positives = 20/31 (64%)
 Frame = +1

Query: 43  HSNTIKLQKEKKKSNHVSELGTRKHRQGRDK 135
           H   +K  +E+  S++ SELG +KH + +++
Sbjct: 26  HEEALKKLREENFSSNTSELGNKKHYRAQER 56



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>YIID_ECOLI (P0ADQ2) Hypothetical protein yiiD|
          Length = 329

 Score = 27.7 bits (60), Expect = 7.0
 Identities = 15/36 (41%), Positives = 19/36 (52%)
 Frame = -1

Query: 378 LFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADE 271
           +FGD  P A     GTY V   K  G + EGG+ +E
Sbjct: 296 IFGDETPGAVFE--GTYIVLPAKPFGPYEEGGNEEE 329



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>YIID_ECOL6 (P0ADQ3) Hypothetical protein yiiD|
          Length = 329

 Score = 27.7 bits (60), Expect = 7.0
 Identities = 15/36 (41%), Positives = 19/36 (52%)
 Frame = -1

Query: 378 LFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADE 271
           +FGD  P A     GTY V   K  G + EGG+ +E
Sbjct: 296 IFGDETPGAVFE--GTYIVLPAKPFGPYEEGGNEEE 329



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>YIID_ECO57 (P0ADQ4) Hypothetical protein yiiD|
          Length = 329

 Score = 27.7 bits (60), Expect = 7.0
 Identities = 15/36 (41%), Positives = 19/36 (52%)
 Frame = -1

Query: 378 LFGDNDPAAAKPKFGTYWVKDGKVIGVFLEGGSADE 271
           +FGD  P A     GTY V   K  G + EGG+ +E
Sbjct: 296 IFGDETPGAVFE--GTYIVLPAKPFGPYEEGGNEEE 329


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,889,383
Number of Sequences: 219361
Number of extensions: 676268
Number of successful extensions: 2408
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 2371
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2405
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 1396778976
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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