ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart52g01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1P2RX5_HUMAN (Q93086) P2X purinoceptor 5 (ATP receptor) (P2X5) (P... 30 1.1
2TRM1_MOUSE (Q3TX08) N(2),N(2)-dimethylguanosine tRNA methyltrans... 29 2.5
3MARE1_HUMAN (Q15691) Microtubule-associated protein RP/EB family... 28 4.3
4YM8C_YEAST (P38430) Hypothetical 36.1 kDa protein in TPS3-IPP2 i... 28 4.3
5DNAK_MYCPA (Q00488) Chaperone protein dnaK (Heat shock protein 7... 28 5.7
6AT10B_HUMAN (O94823) Probable phospholipid-transporting ATPase V... 28 5.7
7VGLL_PRVKA (P52512) Glycoprotein L precursor 28 5.7
8KRA53_HUMAN (Q6L8H2) Keratin-associated protein 5-3 (Keratin-ass... 28 7.4
9YIZI_SCHPO (Q9US42) Hypothetical protein C1002.18 in chromosome I 28 7.4
10FSTL1_RAT (Q62632) Follistatin-related protein 1 precursor (Foll... 28 7.4
11FSTL1_MOUSE (Q62356) Follistatin-related protein 1 precursor (Fo... 28 7.4
12KRA54_HUMAN (Q6L8H1) Keratin-associated protein 5-4 (Keratin-ass... 27 9.7
13KRA52_HUMAN (Q701N4) Keratin-associated protein 5-2 (Keratin-ass... 27 9.7
14KRA51_HUMAN (Q6L8H4) Keratin-associated protein 5-1 (Keratin-ass... 27 9.7
15CUP52_GALME (Q24998) Pupal cuticle protein PCP52 precursor (GMPC... 27 9.7
16YL185_MIMIV (Q5URB5) Hypothetical protein L185 27 9.7
17KRA58_HUMAN (O75690) Keratin-associated protein 5-8 (Keratin-ass... 27 9.7
18KDGD_PSEPK (Q88GW8) 5-dehydro-4-deoxyglucarate dehydratase (EC 4... 27 9.7
19KR511_HUMAN (Q6L8G4) Keratin-associated protein 5-11 (Keratin-as... 27 9.7

>P2RX5_HUMAN (Q93086) P2X purinoceptor 5 (ATP receptor) (P2X5) (Purinergic|
           receptor)
          Length = 421

 Score = 30.4 bits (67), Expect = 1.1
 Identities = 15/55 (27%), Positives = 25/55 (45%)
 Frame = +3

Query: 3   SQTEAITSTGKNVPQK*LTKTDAAQAGRTTRIGGAVKSGPILYRENECRANGDIY 167
           +Q + + +  + +P    +K     AG     G  VK+G  L REN  R   +I+
Sbjct: 112 NQRQNVCAENEGIPDGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIF 166



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>TRM1_MOUSE (Q3TX08) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
           2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
           (tRNA 2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase)
          Length = 663

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = +1

Query: 67  MLRKQEEQPE*VVLLNPARFCTEKTSAEPMGTFT 168
           M RK ++ P    + N  RFC E+  A+P+ T T
Sbjct: 26  MERKAQKPPSPPAMENGTRFCEERPPADPVATVT 59



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>MARE1_HUMAN (Q15691) Microtubule-associated protein RP/EB family member 1|
           (APC-binding protein EB1) (End-binding protein 1) (EB1)
          Length = 267

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
 Frame = +3

Query: 15  AITSTGKNVPQK*LTKTDAAQAG--RTTRIGGAVKSGPILYRENECRANGD 161
           ++ +   N P+K LT + AA      T R   A K+GP + R+N    NGD
Sbjct: 139 SLVAPALNKPKKPLTSSSAAPQRPISTQRTAAAPKAGPGVVRKNPGVGNGD 189



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>YM8C_YEAST (P38430) Hypothetical 36.1 kDa protein in TPS3-IPP2 intergenic|
           region (ORF3)
          Length = 313

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = +1

Query: 202 NWLVDEYCHALVDPNNRSDHPLRADDGNIEG 294
           N LVD +CH + DP    D+    DDG  +G
Sbjct: 2   NKLVDAHCHVITDP----DNTFCGDDGGSQG 28



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>DNAK_MYCPA (Q00488) Chaperone protein dnaK (Heat shock protein 70) (Heat shock|
           70 kDa protein) (HSP70) (70 kDa antigen)
          Length = 622

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 24/70 (34%), Positives = 31/70 (44%), Gaps = 8/70 (11%)
 Frame = +3

Query: 30  GKNVPQK*LTKTDAAQAGRTTRIGG----AVKSGPILYRENECRANGDIYKC----SATK 185
           G  VP++ L+K DAA A   T +GG    A+KS      +        IY+     SA  
Sbjct: 532 GSKVPEETLSKVDAAIADAKTALGGTDITAIKSAMEKLGQESQALGQAIYEATQAESAQA 591

Query: 186 GG*ARQLAGG 215
           GG     AGG
Sbjct: 592 GGPDGAAAGG 601



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>AT10B_HUMAN (O94823) Probable phospholipid-transporting ATPase VB (EC 3.6.3.1)|
          Length = 1461

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = -2

Query: 74   RSICFCKLLLWYIFACGCDS 15
            +++C+  LL WY F CG  S
Sbjct: 1119 KNVCYVNLLFWYQFFCGFSS 1138



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>VGLL_PRVKA (P52512) Glycoprotein L precursor|
          Length = 156

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 13/46 (28%), Positives = 23/46 (50%)
 Frame = -3

Query: 145 HSFSLYRIGPDLTAPPILVVLPACAASVFVSYFCGTFLPVDVIASV 8
           H +  Y + PD+TA   +VV P C+    + +  GT   V+   ++
Sbjct: 72  HPYIAYSLPPDMTAVTSVVVKPYCSPPEVILWASGTAYLVNPFVAI 117



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>KRA53_HUMAN (Q6L8H2) Keratin-associated protein 5-3 (Keratin-associated protein|
           5.3) (Ultrahigh sulfur keratin-associated protein 5.3)
           (Keratin-associated protein 5-9) (Keratin-associated
           protein 5.9) (UHS KerB-like)
          Length = 238

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 1/29 (3%)
 Frame = -2

Query: 98  YSGCSS-CLRSICFCKLLLWYIFACGCDS 15
           Y GC S C   +C CK +   + AC C S
Sbjct: 28  YGGCGSGCCVPVCCCKPVCCCVPACSCSS 56



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>YIZI_SCHPO (Q9US42) Hypothetical protein C1002.18 in chromosome I|
          Length = 399

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 14/38 (36%), Positives = 18/38 (47%)
 Frame = +2

Query: 155 WGHLQMLGN*GWLSKTIGWWMNIAMPWWIQTIDQITPF 268
           W  LQ L    W  +T     N+   W++ TID I PF
Sbjct: 227 WSELQTLLIPIWPVRTSFEGQNLGDAWYLSTIDAIQPF 264



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>FSTL1_RAT (Q62632) Follistatin-related protein 1 precursor (Follistatin-like|
           1)
          Length = 306

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 7/14 (50%), Positives = 9/14 (64%)
 Frame = -1

Query: 171 ICKCPHWLCTRFLC 130
           +C C HW+CT   C
Sbjct: 252 VCSCGHWVCTAMTC 265



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>FSTL1_MOUSE (Q62356) Follistatin-related protein 1 precursor (Follistatin-like|
           1) (TGF-beta-inducible protein TSC-36)
          Length = 306

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 7/14 (50%), Positives = 9/14 (64%)
 Frame = -1

Query: 171 ICKCPHWLCTRFLC 130
           +C C HW+CT   C
Sbjct: 252 VCSCGHWVCTAMTC 265



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>KRA54_HUMAN (Q6L8H1) Keratin-associated protein 5-4 (Keratin-associated protein|
           5.4) (Ultrahigh sulfur keratin-associated protein 5.4)
          Length = 288

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 14/31 (45%), Positives = 16/31 (51%), Gaps = 4/31 (12%)
 Frame = -2

Query: 95  SGC----SSCLRSICFCKLLLWYIFACGCDS 15
           SGC    SSC   IC CK +   + AC C S
Sbjct: 40  SGCGGCGSSCCVPICCCKPVCCCVPACSCSS 70



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>KRA52_HUMAN (Q701N4) Keratin-associated protein 5-2 (Keratin-associated protein|
           5.2) (Ultrahigh sulfur keratin-associated protein 5.2)
           (Keratin-associated protein 5-8) (Keratin-associated
           protein 5.8)
          Length = 177

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
 Frame = -2

Query: 107 STTYSGCSS-CLRSICFCKLLLWYIFACGCDS 15
           S++  GC S C   +C CK +  ++ AC C S
Sbjct: 46  SSSCGGCGSRCYVPVCCCKPVCSWVPACSCTS 77



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>KRA51_HUMAN (Q6L8H4) Keratin-associated protein 5-1 (Keratin-associated protein|
           5.1) (Ultrahigh sulfur keratin-associated protein 5.1)
          Length = 278

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 4/31 (12%)
 Frame = -2

Query: 95  SGC----SSCLRSICFCKLLLWYIFACGCDS 15
           SGC    SSC   +C CK +   + AC C S
Sbjct: 121 SGCGGCGSSCCVPVCCCKPMCCCVPACSCSS 151



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>CUP52_GALME (Q24998) Pupal cuticle protein PCP52 precursor (GMPCP52)|
          Length = 353

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
 Frame = -3

Query: 211 PANCLA*PPLVAEHL*MSPLALHSFSL---YRIGPDLTAPPILVVLPACAASVFV 56
           PA+ +A  PL A  L  SPL L + +L   +  GP    P +  + P    + FV
Sbjct: 291 PASAIAAAPLTAASLIASPLTLPTLNLEQQWVTGPVFVQPGLKAISPISLQTPFV 345



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>YL185_MIMIV (Q5URB5) Hypothetical protein L185|
          Length = 392

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 14/39 (35%), Positives = 16/39 (41%)
 Frame = -2

Query: 137 FSVQNRAGFNSTTYSGCSSCLRSICFCKLLLWYIFACGC 21
           F V  R    + T S C  C    C+CK    YIF   C
Sbjct: 63  FIVGKRIHCQTNTISNCECCTNYTCYCK----YIFQNRC 97



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>KRA58_HUMAN (O75690) Keratin-associated protein 5-8 (Keratin-associated protein|
           5.8) (Ultrahigh sulfur keratin-associated protein 5.8)
           (Keratin, ultra high-sulfur matrix protein B) (UHS
           keratin B) (UHS KerB)
          Length = 187

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 14/31 (45%), Positives = 16/31 (51%), Gaps = 4/31 (12%)
 Frame = -2

Query: 95  SGC----SSCLRSICFCKLLLWYIFACGCDS 15
           SGC    SSC   IC CK +   + AC C S
Sbjct: 19  SGCGGCGSSCCVPICCCKPVCCCVPACSCSS 49



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>KDGD_PSEPK (Q88GW8) 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41)|
           (5-keto-4-deoxy-glucarate dehydratase) (KDGDH)
          Length = 303

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 3/37 (8%)
 Frame = +3

Query: 63  TDAAQAGRTTRIGGAVKS---GPILYRENECRANGDI 164
           T+A+Q G    +    KS   G ++Y  N CR N D+
Sbjct: 116 TEASQDGVAAHVEAVCKSVNIGVVVYNRNVCRLNADL 152



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>KR511_HUMAN (Q6L8G4) Keratin-associated protein 5-11 (Keratin-associated|
           protein 5.11) (Ultrahigh sulfur keratin-associated
           protein 5.11)
          Length = 156

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 14/31 (45%), Positives = 16/31 (51%), Gaps = 4/31 (12%)
 Frame = -2

Query: 95  SGC----SSCLRSICFCKLLLWYIFACGCDS 15
           SGC    SSC   IC CK +   + AC C S
Sbjct: 26  SGCGGCGSSCCVPICCCKPVCCCVPACSCSS 56


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,241,888
Number of Sequences: 219361
Number of extensions: 1092537
Number of successful extensions: 2836
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 2780
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2835
length of database: 80,573,946
effective HSP length: 87
effective length of database: 61,489,539
effective search space used: 1475748936
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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