ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbart49d02 |
|---|---|
| Clone Library Name | barley_pub |
>ERG25_SCHPO (Q9UUH4) C-4 methylsterol oxidase (EC 1.14.13.72) (Methylsterol| monooxygenase) Length = 300 Score = 48.9 bits (115), Expect = 6e-06 Identities = 21/53 (39%), Positives = 31/53 (58%) Frame = -2 Query: 442 FPWSPSNFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGTDKGYRKTK 284 FPWS + FLP++ G D HDYHH N++ +F + D + TD+ Y + K Sbjct: 235 FPWSLNKFLPIWAGADHHDYHHMAF---KDNFSSSFRWWDAVLKTDQNYHQFK 284
>ERG25_PONPY (Q5R574) C-4 methylsterol oxidase (EC 1.14.13.72) (Methylsterol| monooxygenase) Length = 293 Score = 48.5 bits (114), Expect = 7e-06 Identities = 22/48 (45%), Positives = 27/48 (56%) Frame = -2 Query: 439 PWSPSNFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGTDKGY 296 P +P N +P Y G HD+HH GNYA TF + D +FGTD Y Sbjct: 234 PLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDRIFGTDSQY 278
>ERG25_HUMAN (Q15800) C-4 methylsterol oxidase (EC 1.14.13.72) (Methylsterol| monooxygenase) Length = 293 Score = 48.5 bits (114), Expect = 7e-06 Identities = 22/48 (45%), Positives = 27/48 (56%) Frame = -2 Query: 439 PWSPSNFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGTDKGY 296 P +P N +P Y G HD+HH GNYA TF + D +FGTD Y Sbjct: 234 PLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDRIFGTDSQY 278
>ERG25_RAT (O35532) C-4 methylsterol oxidase (EC 1.14.13.72) (Methylsterol| monooxygenase) (Neuropep 1) (RANP-1) Length = 293 Score = 48.1 bits (113), Expect = 1e-05 Identities = 22/48 (45%), Positives = 28/48 (58%) Frame = -2 Query: 439 PWSPSNFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGTDKGY 296 P +P N++P Y G HD+HH GNYA TF + D +FGTD Y Sbjct: 234 PLNPLNYIPFYTGARHHDFHHMNFI---GNYASTFTWWDRIFGTDVQY 278
>ERG25_MOUSE (Q9CRA4) C-4 methylsterol oxidase (EC 1.14.13.72) (Methylsterol| monooxygenase) Length = 293 Score = 47.8 bits (112), Expect = 1e-05 Identities = 23/48 (47%), Positives = 27/48 (56%) Frame = -2 Query: 439 PWSPSNFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGTDKGY 296 P +P N +P Y G HD+HH GNYA TF + D LFGTD Y Sbjct: 234 PLNPLNLVPFYTGARHHDFHHMNFI---GNYASTFTWWDKLFGTDAQY 278
>ERG25_CANAL (O59933) C-4 methylsterol oxidase (EC 1.14.13.72) (Methylsterol| monooxygenase) Length = 308 Score = 47.8 bits (112), Expect = 1e-05 Identities = 24/61 (39%), Positives = 32/61 (52%) Frame = -2 Query: 442 FPWSPSNFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGTDKGYRKTKTMEGEEG 263 FPWS NFLP + G D HD HH G Y+ +F + D++ T+ G K +G E Sbjct: 241 FPWSLHNFLPFWAGADHHDEHHHYFI---GGYSSSFRWWDFILDTEAG---PKAKKGRED 294 Query: 262 K 260 K Sbjct: 295 K 295
>ERG25_YEAST (P53045) C-4 methylsterol oxidase (EC 1.14.13.72) (Methylsterol| monooxygenase) Length = 309 Score = 43.1 bits (100), Expect = 3e-04 Identities = 18/48 (37%), Positives = 26/48 (54%) Frame = -2 Query: 442 FPWSPSNFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGTDKG 299 FPWS + +P + G + HD HH GNYA +F + D+ T+ G Sbjct: 241 FPWSLNKIMPFWAGAEHHDLHHHYFI---GNYASSFRWWDYCLDTESG 285
>C25HA_BRARE (Q5PRC0) Cholesterol 25-hydroxylase-like protein A (EC 1.14.99.-)| Length = 251 Score = 38.5 bits (88), Expect = 0.008 Identities = 20/46 (43%), Positives = 24/46 (52%), Gaps = 2/46 (4%) Frame = -2 Query: 439 PWSPSNFLP--LYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGT 308 PW+ +P LYGG HD HH+ NYA F + D LFGT Sbjct: 205 PWATHRLMPFGLYGGAPHHDVHHQKF---KSNYAPYFTHWDKLFGT 247
>C25HB_BRARE (Q567X1) Cholesterol 25-hydroxylase-like protein B (EC 1.14.99.-)| Length = 282 Score = 35.4 bits (80), Expect = 0.065 Identities = 22/60 (36%), Positives = 32/60 (53%), Gaps = 2/60 (3%) Frame = -2 Query: 439 PWSPSNFLP--LYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGTDKGYRKTKTMEGEE 266 P+SP + +P L GG HD HH+ S N+A F + D +FGT + T+ E E+ Sbjct: 223 PFSPGHLVPFGLLGGAMAHDMHHQ---KPSSNFAPFFSHWDKIFGTAITVKLTQKSEKEK 279
>CH25H_PIG (Q4G1G8) Cholesterol 25-hydroxylase (EC 1.14.99.38) (Cholesterol| 25-monooxygenase) Length = 270 Score = 34.3 bits (77), Expect = 0.14 Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 2/47 (4%) Frame = -2 Query: 442 FPWSPSNFLPL--YGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGT 308 FPWS +P YGG HD HH + N+A F + D + GT Sbjct: 220 FPWSAHRLVPFGWYGGVTHHDLHHSQF---NCNFAPYFTHWDKILGT 263
>CH25H_RAT (Q4QQV7) Cholesterol 25-hydroxylase (EC 1.14.99.38) (Cholesterol| 25-monooxygenase) Length = 298 Score = 33.9 bits (76), Expect = 0.19 Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 2/47 (4%) Frame = -2 Query: 442 FPWSPSNFLPL--YGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGT 308 FPWS +P YGG HD HH + N+A F + D + GT Sbjct: 220 FPWSTHRLVPFGWYGGVAHHDLHHSQF---NCNFAPYFTHWDKMLGT 263
>CH25H_MOUSE (Q9Z0F5) Cholesterol 25-hydroxylase (EC 1.14.99.38) (Cholesterol| 25-monooxygenase) (m25OH) Length = 298 Score = 33.9 bits (76), Expect = 0.19 Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 2/47 (4%) Frame = -2 Query: 442 FPWSPSNFLPL--YGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGT 308 FPWS +P YGG HD HH + N+A F + D + GT Sbjct: 220 FPWSTHRLVPFGWYGGVAHHDMHHSQF---NCNFAPYFTHWDKMLGT 263
>CH25H_HUMAN (O95992) Cholesterol 25-hydroxylase (EC 1.14.99.38) (Cholesterol| 25-monooxygenase) (h25OH) Length = 272 Score = 33.5 bits (75), Expect = 0.25 Identities = 18/48 (37%), Positives = 24/48 (50%), Gaps = 2/48 (4%) Frame = -2 Query: 445 HFPWSPSNFLPL--YGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGT 308 +FPWS +P YGG HD HH + N+A F + D + GT Sbjct: 219 NFPWSTHRLVPFGWYGGVVHHDLHHSHF---NCNFAPYFTHWDKILGT 263
>SC5D_TOBAC (Q9ZT29) Delta-7-sterol-C5(6)-desaturase (EC 1.3.3.-) (Delta-7-C-5| sterol desaturase) (Delta7-sterol-C5-desaturase) Length = 271 Score = 32.7 bits (73), Expect = 0.42 Identities = 14/32 (43%), Positives = 18/32 (56%) Frame = -2 Query: 403 GFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGT 308 G +H HHR T NY ++MDW+FGT Sbjct: 231 GAGYHTIHHR---TYRHNYGHYTIWMDWMFGT 259
>SC5D2_ARATH (Q9M883) Putative delta-7-sterol-C5(6)-desaturase 2 (EC 1.3.3.-)| (Delta-7-C-5 sterol desaturase 2) (Delta7-sterol-C5-desaturase 2) (Homolog of DWF7 protein) Length = 279 Score = 30.0 bits (66), Expect = 2.7 Identities = 14/39 (35%), Positives = 20/39 (51%) Frame = -2 Query: 424 NFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGT 308 N P+ G +H HH T NY ++MDW+FG+ Sbjct: 229 NIWPIMGA-GYHTIHHT---TYKHNYGHYTIWMDWMFGS 263
>SYE_YERPS (Q668N0) Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA| ligase) (GluRS) Length = 471 Score = 29.6 bits (65), Expect = 3.5 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -3 Query: 195 PVLVITLSSWEERRRGIDPRHGPSIVHVVEFL 100 P V SW + GID R+GP +V +V+ L Sbjct: 316 PEQVAVHLSWHVEQLGIDTRNGPELVEIVKLL 347
>SYE_YERPE (Q8ZCK0) Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA| ligase) (GluRS) Length = 471 Score = 29.6 bits (65), Expect = 3.5 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -3 Query: 195 PVLVITLSSWEERRRGIDPRHGPSIVHVVEFL 100 P V SW + GID R+GP +V +V+ L Sbjct: 316 PEQVAVHLSWHVEQLGIDTRNGPELVEIVKLL 347
>SC5D1_ARATH (Q39208) Delta-7-sterol-C5(6)-desaturase 1 (EC 1.3.3.-)| (Delta-7-C-5 sterol desaturase 1) (Delta7-sterol-C5-desaturase 1) (STEROL1 protein) (Dwarf 7 protein) Length = 281 Score = 29.6 bits (65), Expect = 3.5 Identities = 14/39 (35%), Positives = 20/39 (51%) Frame = -2 Query: 424 NFLPLYGGFDFHDYHHRVLYTKSGNYAFTFVYMDWLFGT 308 N P+ G +H HH T NY ++MDW+FG+ Sbjct: 228 NIWPVMGA-GYHTIHHT---TYKHNYGHYTIWMDWMFGS 262
>RDRP_PVXX3 (P17779) RNA replication protein (165 kDa protein) (ORF 1 protein)| [Includes: RNA-directed RNA polymerase (EC 2.7.7.48); Helicase (EC 3.6.1.-)] Length = 1456 Score = 29.3 bits (64), Expect = 4.6 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = -2 Query: 247 VEATFFFESTHQN*FSISCIGDNIVFMGG 161 VEA F ESTH N +S+ GD ++ G Sbjct: 179 VEAAFKMESTHPNIYSLKYFGDGFQYIPG 207
>RDRP_PVXHB (Q07630) RNA replication protein (165 kDa protein) (ORF 1 protein)| [Includes: RNA-directed RNA polymerase (EC 2.7.7.48); Helicase (EC 3.6.1.-)] Length = 1456 Score = 29.3 bits (64), Expect = 4.6 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = -2 Query: 247 VEATFFFESTHQN*FSISCIGDNIVFMGG 161 VEA F ESTH N +S+ GD ++ G Sbjct: 179 VEAAFKMESTHPNIYSLKYFGDGFQYIPG 207
>RDRP_PVX (P09395) RNA replication protein (165 kDa protein) (ORF 1 protein)| [Includes: RNA-directed RNA polymerase (EC 2.7.7.48); Helicase (EC 3.6.1.-)] Length = 1456 Score = 29.3 bits (64), Expect = 4.6 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = -2 Query: 247 VEATFFFESTHQN*FSISCIGDNIVFMGG 161 VEA F ESTH N +S+ GD ++ G Sbjct: 179 VEAAFKMESTHPNIYSLKYFGDGFQYIPG 207
>MEGF8_RAT (Q9QYP0) Multiple epidermal growth factor-like domains 8 (EGF-like| domain-containing protein 4) (Multiple EGF-like domain protein 4) (Fragment) Length = 874 Score = 29.3 bits (64), Expect = 4.6 Identities = 15/48 (31%), Positives = 18/48 (37%) Frame = +1 Query: 289 SCDNPYLCQTTSPCKQK*RRSSLTWCKAHDDGSHGSQSLHTVAGSLMG 432 +C C S C R C+ H GSH Q L GS +G Sbjct: 277 NCSTECRCNRHSECAGVGARDHCLLCRNHTKGSHCEQCLPLFVGSALG 324
>POLG_PEV9U (O41174) Genome polyprotein [Contains: Coat protein VP4 (P1A); Coat| protein VP2 (P1B); Coat protein VP3 (P1C); Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29) (Core protein P2A); Core protein P2B; Core protein P2C; Core protein P3A; Genome Length = 2167 Score = 29.3 bits (64), Expect = 4.6 Identities = 18/69 (26%), Positives = 29/69 (42%), Gaps = 2/69 (2%) Frame = -3 Query: 438 LGAHQTSCHCMEALTSMTTIIVCFTPSQGTTPLLLFTWT--GCLAQIRVIARQKPWKGKK 265 +GA C++ + + +V T SQG P +L T GC PW+ K Sbjct: 1017 IGADGMVERCLQTFVKVISAVVIATRSQGDVPTILATLALIGC--------DGSPWRWLK 1068 Query: 264 GSICKLWKL 238 C ++K+ Sbjct: 1069 RQFCGIFKI 1077
>MEGF8_HUMAN (Q7Z7M0) Multiple epidermal growth factor-like domains 8 (EGF-like| domain-containing protein 4) (Multiple EGF-like domain protein 4) Length = 2386 Score = 28.9 bits (63), Expect = 6.0 Identities = 15/48 (31%), Positives = 18/48 (37%) Frame = +1 Query: 289 SCDNPYLCQTTSPCKQK*RRSSLTWCKAHDDGSHGSQSLHTVAGSLMG 432 +C C S C R C+ H GSH Q L GS +G Sbjct: 1789 NCSTECRCNRHSECAGVGARDHCLLCRNHTKGSHCEQCLPLFVGSAVG 1836
>IKKB_RAT (Q9QY78) Inhibitor of nuclear factor kappa B kinase beta subunit| (EC 2.7.11.1) (I-kappa-B-kinase beta) (IkBKB) (IKK-beta) (IKK-B) (I-kappa-B kinase 2) (IKK2) (Nuclear factor NF-kappa-B inhibitor kinase beta) (NFKBIKB) Length = 757 Score = 28.9 bits (63), Expect = 6.0 Identities = 8/16 (50%), Positives = 15/16 (93%) Frame = -3 Query: 171 SWEERRRGIDPRHGPS 124 +W+ R+RG+DP++GP+ Sbjct: 282 TWQPRQRGVDPQYGPN 297 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 71,398,662 Number of Sequences: 219361 Number of extensions: 1499651 Number of successful extensions: 3479 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 3391 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3472 length of database: 80,573,946 effective HSP length: 102 effective length of database: 58,199,124 effective search space used: 2677159704 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
