ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart48c01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GEK1_ARATH (Q9ZPQ3) Ethanol tolerance protein GEKO1 109 4e-24
2PNO_EUGGR (Q94IN5) Pyruvate dehydrogenase [NADP+], mitochondrial... 30 5.6
3MRP2_ARATH (Q42093) Multidrug resistance-associated protein 2 (E... 29 7.3
4LGT_LEPIN (Q8F1X4) Prolipoprotein diacylglyceryl transferase (EC... 29 7.3
5LGT_LEPIC (Q72TF8) Prolipoprotein diacylglyceryl transferase (EC... 29 7.3
6NUD1_YEAST (P32336) Protein NUD1 29 9.5
7L_NDVB (P11205) Large structural protein (Protein L) (Transcript... 29 9.5

>GEK1_ARATH (Q9ZPQ3) Ethanol tolerance protein GEKO1|
          Length = 317

 Score =  109 bits (273), Expect = 4e-24
 Identities = 51/83 (61%), Positives = 64/83 (77%), Gaps = 2/83 (2%)
 Frame = -2

Query: 535 LLSGYSLPMDAPAQVNGKSSGE--VGGMWKHSIQVSYEAAKAGFPGGEVIAHLDQKSFKG 362
           LLSGYSLPM+ P Q    + GE  +GG W+ SI+ ++EA KA FPGGE++AHLD KSFKG
Sbjct: 235 LLSGYSLPMEDPTQTK-TTPGENYIGGNWRQSIKAAFEATKASFPGGEILAHLDHKSFKG 293

Query: 361 WQKNAITSYLQEQNIRIGKPNDF 293
           WQK AIT +L E++I +GKPNDF
Sbjct: 294 WQKKAITEFLAEESINVGKPNDF 316



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>PNO_EUGGR (Q94IN5) Pyruvate dehydrogenase [NADP+], mitochondrial precursor|
           (EC 1.2.1.51) (EC 1.16.1.5) (Pyruvate:NADP+
           oxidoreductase) (EgPNOmt) (Aquacobalamin reductase
           [NADPH])
          Length = 1803

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 30/104 (28%), Positives = 41/104 (39%), Gaps = 19/104 (18%)
 Frame = -2

Query: 421 KAGFPGGEVIAHLDQKSFKGWQKNAITSYLQEQNIRI------------GKPNDF-LCTK 281
           K G   G V+ HL    ++ W   A    L +   RI            G+P    +   
Sbjct: 356 KRGKKVGAVLVHL----YRPWSTKAFEKVLPKTVKRIAALDRCKEVTALGEPLYLDVSAT 411

Query: 280 I*LLPQRKVVKVTSSSLGLGT------HKLSRYRALCLNNPISR 167
           + L P+R+ VKV     GLG+      H L+ Y  L   NPI R
Sbjct: 412 LNLFPERQNVKVIGGRYGLGSKDFIPEHALAIYANLASENPIQR 455



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>MRP2_ARATH (Q42093) Multidrug resistance-associated protein 2 (EC 3.6.3.44)|
           (Glutathione S-conjugate transporting ATPase 2)
           (ATP-energized glutathione S-conjugate pump 2)
          Length = 1623

 Score = 29.3 bits (64), Expect = 7.3
 Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 2/58 (3%)
 Frame = +1

Query: 25  SANSWNHKHA*SGRQTCPEDTSLIYSWFFFSFGAKVSPSKTNGRKHQVLKSD--YLNT 192
           + + + ++    G+Q CPE  + I+   FFS+   ++P  T G K  + + D  YL+T
Sbjct: 210 TVDDYEYEEISDGQQICPEKHANIFDKIFFSW---MNPLMTLGSKRPLTEKDVWYLDT 264



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>LGT_LEPIN (Q8F1X4) Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-)|
          Length = 318

 Score = 29.3 bits (64), Expect = 7.3
 Identities = 11/33 (33%), Positives = 18/33 (54%)
 Frame = +3

Query: 42  PQARVVWQTNVS*RYVSHIFLVFFLFWRKSQSF 140
           P    VW T +    +S +F  +F FW ++Q+F
Sbjct: 195 PSGVPVWNTPLIESIISFLFFFYFQFWARNQNF 227



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>LGT_LEPIC (Q72TF8) Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-)|
          Length = 318

 Score = 29.3 bits (64), Expect = 7.3
 Identities = 11/33 (33%), Positives = 18/33 (54%)
 Frame = +3

Query: 42  PQARVVWQTNVS*RYVSHIFLVFFLFWRKSQSF 140
           P    VW T +    +S +F  +F FW ++Q+F
Sbjct: 195 PSGVPVWNTPLIESIISFLFFFYFQFWARNQNF 227



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>NUD1_YEAST (P32336) Protein NUD1|
          Length = 851

 Score = 28.9 bits (63), Expect = 9.5
 Identities = 21/53 (39%), Positives = 31/53 (58%), Gaps = 1/53 (1%)
 Frame = +1

Query: 40  NHKHA*SGRQTCPEDTSLIYSWFFFSFGAKVSPSKT-NGRKHQVLKSDYLNTV 195
           N+K     ++   EDTS   + F FS  + +S S+T +GRK +VLK   LNT+
Sbjct: 244 NNKDNARTKEEDEEDTS---NSFEFSSSSSMSSSQTQSGRKSKVLKKPPLNTI 293



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>L_NDVB (P11205) Large structural protein (Protein L) (Transcriptase)|
            (Replicase) [Includes: RNA-directed RNA polymerase (EC
            2.7.7.48); mRNA (guanine-N(7)-)-methyltransferase (EC
            2.1.1.56); mRNA guanylyltransferase (EC 2.7.7.-)]
          Length = 2204

 Score = 28.9 bits (63), Expect = 9.5
 Identities = 26/81 (32%), Positives = 33/81 (40%), Gaps = 1/81 (1%)
 Frame = +1

Query: 49   HA*SGRQTCPEDTSLIYSWFFFSFGAKVSPSKTNGRKHQVLKSDYL-NTVLYIYSICACQ 225
            H    R+T   DT  IYS   F  GA +S    N  K  ++  D   NTV+   +I +  
Sbjct: 794  HNLKDRETIRSDTFFIYSKRIFKDGAILSQVLKNSSKLVMVSGDLSENTVMSCANIASTV 853

Query: 226  ALGRMKSL*RLCAVGAVKFLC 288
            A        RLC  G  K  C
Sbjct: 854  A--------RLCENGLPKDFC 866


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,003,152
Number of Sequences: 219361
Number of extensions: 1732593
Number of successful extensions: 4551
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 4421
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4550
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4200495993
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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