ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart46e07
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NRL4_TOBAC (Q42965) Nitrilase 4 (EC 3.5.5.1) 144 2e-34
2NRL4_ARATH (P46011) Nitrilase 4 (EC 3.5.5.1) 140 2e-33
3NRL2_ARATH (P32962) Nitrilase 2 (EC 3.5.5.1) 112 6e-25
4NRL3_ARATH (P46010) Nitrilase 3 (EC 3.5.5.1) 104 1e-22
5NRL1_ARATH (P32961) Nitrilase 1 (EC 3.5.5.1) 100 4e-21
6YIQ5_YEAST (P40446) Hypothetical nitrilase-like protein YIL165C 53 6e-07
7NRL1_RHORH (Q02068) Aliphatic nitrilase (EC 3.5.5.7) 46 7e-05
8NRLB_KLEPO (P10045) Nitrilase, bromoxynil-specific (EC 3.5.5.1) 42 0.001
9NRL2_RHORH (Q03217) Aliphatic nitrilase (EC 3.5.5.7) 41 0.002
10CYHY_GIBBA (P32963) Cyanide hydratase (EC 4.2.1.66) (Formamide h... 39 0.009
11NRL_BACSX (P82605) Nitrilase (EC 3.5.5.1) 37 0.026
12CYHY_GLOSO (P32964) Cyanide hydratase (EC 4.2.1.66) (Formamide h... 37 0.034
13NRLA_ALCFA (P20960) Nitrilase, arylacetone-specific (EC 3.5.5.1)... 37 0.044
14ADH6_YEAST (Q04894) NADP-dependent alcohol dehydrogenase 6 (EC 1... 30 4.1
15STRN3_RAT (P58405) Striatin-3 (Cell-cycle autoantigen SG2NA) (S/... 30 5.4
16GLIP1_HUMAN (P48060) Glioma pathogenesis-related protein 1 precu... 30 5.4
17CCNB_DROME (P20439) G2/mitotic-specific cyclin-B 30 5.4
18FABH_SPIOL (Q07510) 3-oxoacyl-[acyl-carrier-protein] synthase II... 30 5.4
19YCB8_PSEDE (P29941) Hypothetical 19.2 kDa protein in cobO 3'regi... 29 7.1
20LPXH_CHRVO (Q7NT75) UDP-2,3-diacylglucosamine hydrolase (EC 3.6.... 29 7.1

>NRL4_TOBAC (Q42965) Nitrilase 4 (EC 3.5.5.1)|
          Length = 349

 Score =  144 bits (363), Expect = 2e-34
 Identities = 71/108 (65%), Positives = 80/108 (74%)
 Frame = -1

Query: 516 GMLVLTANQFCLRKDFPPPPEYTFGGHEEEPSPETAVCXXXXXXXXXXGTVLAGPNYEGE 337
           G  VL+ANQFC RKD+PPPPEY F G EE+ +P++ VC          G VLAGPNY GE
Sbjct: 233 GCFVLSANQFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGE 292

Query: 336 ALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVSFTST 193
           AL++ADLDLGEI RAKFDFDVVGHYARPEVLSL V+      VSFTST
Sbjct: 293 ALISADLDLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTST 340



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>NRL4_ARATH (P46011) Nitrilase 4 (EC 3.5.5.1)|
          Length = 355

 Score =  140 bits (353), Expect = 2e-33
 Identities = 66/108 (61%), Positives = 79/108 (73%)
 Frame = -1

Query: 516 GMLVLTANQFCLRKDFPPPPEYTFGGHEEEPSPETAVCXXXXXXXXXXGTVLAGPNYEGE 337
           G  VL+ANQFC RKD+P PPEY F G EE  +P++ VC          G VLAGPNY GE
Sbjct: 240 GCFVLSANQFCRRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGE 299

Query: 336 ALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVSFTST 193
           AL+TADLDLG+I RAKFDFDVVGHY+RPEV SL ++  P+ AVSF ++
Sbjct: 300 ALITADLDLGDIARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSFKTS 347



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>NRL2_ARATH (P32962) Nitrilase 2 (EC 3.5.5.1)|
          Length = 339

 Score =  112 bits (280), Expect = 6e-25
 Identities = 57/109 (52%), Positives = 70/109 (64%)
 Frame = -1

Query: 516 GMLVLTANQFCLRKDFPPPPEYTFGGHEEEPSPETAVCXXXXXXXXXXGTVLAGPNYEGE 337
           G  VL+A QFCLRKDFP  P+Y F    ++  P++ V           G VLAGPN+E E
Sbjct: 222 GCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESE 281

Query: 336 ALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVSFTSTV 190
            L+TADLDLG++ RAK  FD VGHY+RP+VL LTV   PK  V+F S V
Sbjct: 282 GLITADLDLGDVARAKLYFDSVGHYSRPDVLHLTVNEHPKKPVTFISKV 330



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>NRL3_ARATH (P46010) Nitrilase 3 (EC 3.5.5.1)|
          Length = 346

 Score =  104 bits (260), Expect = 1e-22
 Identities = 52/109 (47%), Positives = 68/109 (62%)
 Frame = -1

Query: 516 GMLVLTANQFCLRKDFPPPPEYTFGGHEEEPSPETAVCXXXXXXXXXXGTVLAGPNYEGE 337
           G  VL+A+QFC R++FP  P+Y F    +    +  V           G VLAGPNYE E
Sbjct: 229 GCFVLSAHQFCKRREFPEHPDYLFNDIVDTKEHDPTVSGGGSVIISPLGKVLAGPNYESE 288

Query: 336 ALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVSFTSTV 190
            L+TADLDLG+I RAK  FDVVGHY++P++ +LTV   PK  V+F + V
Sbjct: 289 GLVTADLDLGDIARAKLYFDVVGHYSKPDIFNLTVNEHPKKPVTFMTKV 337



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>NRL1_ARATH (P32961) Nitrilase 1 (EC 3.5.5.1)|
          Length = 346

 Score = 99.8 bits (247), Expect = 4e-21
 Identities = 51/109 (46%), Positives = 68/109 (62%)
 Frame = -1

Query: 516 GMLVLTANQFCLRKDFPPPPEYTFGGHEEEPSPETAVCXXXXXXXXXXGTVLAGPNYEGE 337
           G  VL+A QFC RK FP  P+Y F    ++   ++ V           G VLAGPN+E E
Sbjct: 229 GCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSIVSQGGSVIISPLGQVLAGPNFESE 288

Query: 336 ALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVSFTSTV 190
            L+TAD+DLG+I RAK  FD VG+Y+RP+VL LTV   P+ +V+F + V
Sbjct: 289 GLVTADIDLGDIARAKLYFDSVGYYSRPDVLHLTVNEHPRKSVTFVTKV 337



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>YIQ5_YEAST (P40446) Hypothetical nitrilase-like protein YIL165C|
          Length = 119

 Score = 52.8 bits (125), Expect = 6e-07
 Identities = 28/56 (50%), Positives = 35/56 (62%)
 Frame = -1

Query: 366 VLAGPNYEGEALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVSFT 199
           ++AGP    E LLTA+++   I  A+FD D VGHYAR +V  LTV  E  H V FT
Sbjct: 64  IIAGPLLGQEGLLTAEINTDLIAEARFDLDPVGHYARGDVFQLTV-NERSHDVKFT 118



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>NRL1_RHORH (Q02068) Aliphatic nitrilase (EC 3.5.5.7)|
          Length = 383

 Score = 45.8 bits (107), Expect = 7e-05
 Identities = 23/53 (43%), Positives = 32/53 (60%)
 Frame = -1

Query: 345 EGEALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVSFTSTVG 187
           + E LL A+LDL +I+ AK   D  GHY+RP+VLSL + T     V + +  G
Sbjct: 277 DAEGLLYAELDLEQIILAKAAADPAGHYSRPDVLSLKIDTRNHTPVQYITADG 329



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>NRLB_KLEPO (P10045) Nitrilase, bromoxynil-specific (EC 3.5.5.1)|
          Length = 349

 Score = 41.6 bits (96), Expect = 0.001
 Identities = 17/45 (37%), Positives = 30/45 (66%)
 Frame = -1

Query: 339 EALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVS 205
           E ++ A++DL  +  AK+  D  GHY+RP+V S+++  + + AVS
Sbjct: 265 EGIVYAEIDLSMLEAAKYSLDPTGHYSRPDVFSVSINRQRQPAVS 309



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>NRL2_RHORH (Q03217) Aliphatic nitrilase (EC 3.5.5.7)|
          Length = 365

 Score = 40.8 bits (94), Expect = 0.002
 Identities = 20/34 (58%), Positives = 24/34 (70%)
 Frame = -1

Query: 339 EALLTADLDLGEIVRAKFDFDVVGHYARPEVLSL 238
           E +L AD+DL  I  AK   D VGHY+RP+VLSL
Sbjct: 272 EGILYADIDLSAITLAKQAADPVGHYSRPDVLSL 305



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>CYHY_GIBBA (P32963) Cyanide hydratase (EC 4.2.1.66) (Formamide hydrolyase)|
          Length = 357

 Score = 38.9 bits (89), Expect = 0.009
 Identities = 22/75 (29%), Positives = 35/75 (46%)
 Frame = -1

Query: 429 EPSPETAVCXXXXXXXXXXGTVLAGPNYEGEALLTADLDLGEIVRAKFDFDVVGHYARPE 250
           EP  + +V           G+++  P  + + LL  D+DL E    K   D  GHY RP+
Sbjct: 247 EPETDPSVYNGHARIYRPDGSLVVKPEKDFDGLLFVDIDLNECHLTKVLADFAGHYMRPD 306

Query: 249 VLSLTVKTEPKHAVS 205
           ++ L V T  K  ++
Sbjct: 307 LIRLLVDTRRKKLIT 321



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>NRL_BACSX (P82605) Nitrilase (EC 3.5.5.1)|
          Length = 338

 Score = 37.4 bits (85), Expect = 0.026
 Identities = 19/45 (42%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
 Frame = -1

Query: 348 YEGEALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTV-KTEPK 217
           ++ E +  AD+DL +I+  KF  D  GHY+ P  LSL+  +TE K
Sbjct: 264 HDEEGITYADIDLEQIIPGKFLIDSAGHYSTPGFLSLSFDRTEKK 308



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>CYHY_GLOSO (P32964) Cyanide hydratase (EC 4.2.1.66) (Formamide hydrolyase)|
          Length = 368

 Score = 37.0 bits (84), Expect = 0.034
 Identities = 19/55 (34%), Positives = 29/55 (52%)
 Frame = -1

Query: 369 TVLAGPNYEGEALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTVKTEPKHAVS 205
           ++ A P  + + L+  D+DL E    K   D  GHY RP+++ L V T  K  V+
Sbjct: 266 SLYAKPAVDFDGLMYVDIDLNESHLTKALADFAGHYMRPDLIRLLVDTRRKELVT 320



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>NRLA_ALCFA (P20960) Nitrilase, arylacetone-specific (EC 3.5.5.1)|
           (Arylacetonitrilase)
          Length = 356

 Score = 36.6 bits (83), Expect = 0.044
 Identities = 17/39 (43%), Positives = 25/39 (64%)
 Frame = -1

Query: 348 YEGEALLTADLDLGEIVRAKFDFDVVGHYARPEVLSLTV 232
           ++ E L+ ADL++ EI  AK   D VGHY++PE   L +
Sbjct: 268 HDAEGLIIADLNMEEIAFAKAINDPVGHYSKPEATRLVL 306



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>ADH6_YEAST (Q04894) NADP-dependent alcohol dehydrogenase 6 (EC 1.1.1.2)|
           (NADP-dependent alcohol dehydrogenase VI) (ScADHVI)
          Length = 360

 Score = 30.0 bits (66), Expect = 4.1
 Identities = 22/76 (28%), Positives = 35/76 (46%), Gaps = 7/76 (9%)
 Frame = +1

Query: 190 DSRSERHGMFRFSLHGQAQHLRSG----VVSHDVE---IKLGSNNFSKVQVSCEEGLAFI 348
           D + E  G+    +H  A H  +     VV H++    +KLG  + S ++V    G+   
Sbjct: 37  DIKIEACGVCGSDIHCAAGHWGNMKMPLVVGHEIVGKVVKLGPKSNSGLKVGQRVGVGAQ 96

Query: 349 VGTCQHCPRWRNDGAP 396
           V +C  C R +ND  P
Sbjct: 97  VFSCLECDRCKNDNEP 112



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>STRN3_RAT (P58405) Striatin-3 (Cell-cycle autoantigen SG2NA) (S/G2 antigen)|
           (Fragment)
          Length = 461

 Score = 29.6 bits (65), Expect = 5.4
 Identities = 16/39 (41%), Positives = 20/39 (51%)
 Frame = +2

Query: 365 TVPDGEMMELPPGQTAVSGDGSSSWPPKVYSGGGGKSFL 481
           T  DGE      G+   SGDG+    P  +  GGGKSF+
Sbjct: 323 TAEDGE----GAGEARSSGDGTEWAEPITFPSGGGKSFI 357



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>GLIP1_HUMAN (P48060) Glioma pathogenesis-related protein 1 precursor (GliPR 1)|
           (RTVP-1 protein)
          Length = 266

 Score = 29.6 bits (65), Expect = 5.4
 Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = +3

Query: 348 SWDLPALSQMAK*WSSLQDKQRFLGMAPPHG-HQRCTQVGE 467
           +WD PAL+Q+AK W+S         + PPH  H   T +GE
Sbjct: 59  TWD-PALAQIAKAWASNCQFSHNTRLKPPHKLHPNFTSLGE 98



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>CCNB_DROME (P20439) G2/mitotic-specific cyclin-B|
          Length = 530

 Score = 29.6 bits (65), Expect = 5.4
 Identities = 19/48 (39%), Positives = 28/48 (58%)
 Frame = +2

Query: 374 DGEMMELPPGQTAVSGDGSSSWPPKVYSGGGGKSFLRQNWFAVRTSIP 517
           D    + P G T  +G+G+ + PPKV + GG  +FLR N  +VR  +P
Sbjct: 79  DSHWKKQPLGST--NGNGNGAVPPKV-NEGGVSAFLRSN--SVRNRVP 121



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>FABH_SPIOL (Q07510) 3-oxoacyl-[acyl-carrier-protein] synthase III, chloroplast|
           precursor (EC 2.3.1.41) (Beta-ketoacyl-ACP synthase III)
           (KAS III)
          Length = 405

 Score = 29.6 bits (65), Expect = 5.4
 Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
 Frame = +1

Query: 199 SERHGMFRFSLH---GQAQHLRSGVVSHDVEIKLGSN 300
           SE  GMF F LH   G  +HL + +++ + +  +G+N
Sbjct: 240 SEEDGMFAFDLHSDGGGGRHLNASLLNDETDAAIGNN 276



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>YCB8_PSEDE (P29941) Hypothetical 19.2 kDa protein in cobO 3'region (ORF8)|
          Length = 175

 Score = 29.3 bits (64), Expect = 7.1
 Identities = 25/85 (29%), Positives = 37/85 (43%), Gaps = 17/85 (20%)
 Frame = +1

Query: 112 LLYMTIEDYWTSGLENKIIFAPRI------LSDSRSERHGMFRFSLHGQAQHLRSG---- 261
           LL+ T+ED   + LE K  F P++      L+D  +  HG  +       QHL S     
Sbjct: 62  LLHDTVEDTDATLLEIKEAFGPKVATLVAWLTDISTPFHGNRQVRKELDRQHLASAPAAA 121

Query: 262 -------VVSHDVEIKLGSNNFSKV 315
                  ++ + + IK G  NF KV
Sbjct: 122 KTVKLADLIDNAIAIKAGDPNFWKV 146



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>LPXH_CHRVO (Q7NT75) UDP-2,3-diacylglucosamine hydrolase (EC 3.6.1.-)|
          Length = 237

 Score = 29.3 bits (64), Expect = 7.1
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = -2

Query: 464 PHLSTPLVAMRRSHPQKPLFVLEGAPSF 381
           P+L+ PL AMR    Q PL+V+ G   F
Sbjct: 54  PYLAAPLAAMRDFAAQTPLYVMRGNRDF 81


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,197,240
Number of Sequences: 219361
Number of extensions: 1698973
Number of successful extensions: 4609
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 4378
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4606
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4085413911
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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