ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart42e08
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1ADH_MYCPN (P75214) Probable NADP-dependent alcohol dehydrogenase... 32 0.51
2TBB_ENCCU (Q8SS99) Tubulin beta chain (Beta tubulin) 31 0.87
3TBB_ENCIN (Q9GSR5) Tubulin beta chain (Beta tubulin) 30 1.5
4SYR_SALTY (P74871) Arginyl-tRNA synthetase (EC 6.1.1.19) (Argini... 29 2.5
5RAD50_PYRHO (O58687) DNA double-strand break repair rad50 ATPase 29 2.5
6ENTK_HUMAN (P98073) Enteropeptidase precursor (EC 3.4.21.9) (Ent... 29 2.5
7TBB_ENCHE (Q24829) Tubulin beta chain (Beta tubulin) 29 3.3
8NIFJ_SYNY3 (P52965) Putative pyruvate-flavodoxin oxidoreductase ... 28 5.6
9RPA4_YEAST (P46669) DNA-dependent RNA polymerase 36 kDa polypept... 28 7.3
10RAD50_PYRAB (Q9UZC8) DNA double-strand break repair rad50 ATPase 28 7.3
11ENGC_PSESM (Q87VI5) Probable GTPase engC (EC 3.6.1.-) 28 7.3
12SPT6H_DROME (Q9W420) Transcription elongation factor SPT6 28 7.3
13PCLO_MOUSE (Q9QYX7) Protein piccolo (Aczonin) (Multidomain presy... 27 9.6
14PCLO_HUMAN (Q9Y6V0) Protein piccolo (Aczonin) 27 9.6
15MEF2A_MOUSE (Q60929) Myocyte-specific enhancer factor 2A 27 9.6
16PCLO_RAT (Q9JKS6) Protein piccolo (Aczonin) (Multidomain presyna... 27 9.6

>ADH_MYCPN (P75214) Probable NADP-dependent alcohol dehydrogenase (EC 1.1.1.2)|
          Length = 351

 Score = 31.6 bits (70), Expect = 0.51
 Identities = 13/43 (30%), Positives = 27/43 (62%)
 Frame = -3

Query: 305 LPKLDMSVGPLSEIVGGGLKKYKTLRVDEVAKVVFSSKLDGKK 177
           +P+++  VG   + + GGL     LR++++A+++ + KLD  K
Sbjct: 276 IPRVEWGVGMAHKAIHGGLMLGGRLRLEKLARLIMTKKLDPSK 318



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>TBB_ENCCU (Q8SS99) Tubulin beta chain (Beta tubulin)|
          Length = 439

 Score = 30.8 bits (68), Expect = 0.87
 Identities = 13/51 (25%), Positives = 27/51 (52%)
 Frame = -3

Query: 344 NPHKERMSISAFHLPKLDMSVGPLSEIVGGGLKKYKTLRVDEVAKVVFSSK 192
           N    +++++    P+L   V   + ++  G +K+KT  V E+ + +F SK
Sbjct: 247 NADLRKLAVNMIPFPRLHFFVAGFAPLIAIGTQKFKTYSVSELTQQMFDSK 297



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>TBB_ENCIN (Q9GSR5) Tubulin beta chain (Beta tubulin)|
          Length = 439

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 13/51 (25%), Positives = 27/51 (52%)
 Frame = -3

Query: 344 NPHKERMSISAFHLPKLDMSVGPLSEIVGGGLKKYKTLRVDEVAKVVFSSK 192
           N +  +++++    P+L   V   + +   G +K+KT  V E+ + +F SK
Sbjct: 247 NAYLRKLAVNMIPFPRLHFFVVGFAPLTAVGTQKFKTYSVSELTQQMFDSK 297



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>SYR_SALTY (P74871) Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine--tRNA|
           ligase) (ArgRS)
          Length = 577

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 16/60 (26%), Positives = 32/60 (53%), Gaps = 2/60 (3%)
 Frame = -1

Query: 226 WTRLLRLFSRANLTGRKPRTMPCLASESTRAKAWSRNL--AALVAIIEHIGSKHLPCSTL 53
           +TR+L +F +AN+  +   + P + SE   A+  +R L     + ++   G+ H+ C+ L
Sbjct: 464 YTRVLSVFRKANIDEQALASAPVIISEDREAQLAARLLQFEETLTVVAREGTPHVMCAYL 523



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>RAD50_PYRHO (O58687) DNA double-strand break repair rad50 ATPase|
          Length = 879

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 20/59 (33%), Positives = 33/59 (55%), Gaps = 7/59 (11%)
 Frame = -3

Query: 335 KERMSISAFHLPKLDMSVGPLSEIVGGGLKKYKTL-------RVDEVAKVVFSSKLDGK 180
           KER S +   L KL++++  + E+  G +K+YK L       ++ E+A  +FS   DGK
Sbjct: 704 KERES-AKMELEKLNIAIKRIEEL-RGKIKEYKALIKEEALNKIGEIASEIFSEFTDGK 760



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>ENTK_HUMAN (P98073) Enteropeptidase precursor (EC 3.4.21.9) (Enterokinase)|
           [Contains: Enteropeptidase non-catalytic heavy chain;
           Enteropeptidase catalytic light chain]
          Length = 1019

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 19/53 (35%), Positives = 26/53 (49%)
 Frame = -1

Query: 304 SQSWI*VWAHYLK*LEEGSRNTRP*GWTRLLRLFSRANLTGRKPRTMPCLASE 146
           S  W+   AH +       RN  P  WT +L L  ++NLT   P+T+P L  E
Sbjct: 816 SSDWLVSAAHCVY-----GRNLEPSKWTAILGLHMKSNLT--SPQTVPRLIDE 861



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>TBB_ENCHE (Q24829) Tubulin beta chain (Beta tubulin)|
          Length = 439

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 13/51 (25%), Positives = 27/51 (52%)
 Frame = -3

Query: 344 NPHKERMSISAFHLPKLDMSVGPLSEIVGGGLKKYKTLRVDEVAKVVFSSK 192
           N    +++++    P+L   V   + ++  G +K+KT  V E+ + +F SK
Sbjct: 247 NADLRKLAVNMIPFPRLHFFVVGSAPLIAIGTQKFKTYSVSELTQQMFDSK 297



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>NIFJ_SYNY3 (P52965) Putative pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-)|
          Length = 1199

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = +1

Query: 193 LLEKTTLATSSTLRVLYFLSPPPTISDSGPTL 288
           ++E    A  STL  LY +S P T+SD  P +
Sbjct: 597 IVEMNIKAVDSTLAHLYEVSVPETVSDDAPAM 628



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>RPA4_YEAST (P46669) DNA-dependent RNA polymerase 36 kDa polypeptide (EC|
           2.7.7.6) (A43)
          Length = 326

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 12/48 (25%), Positives = 25/48 (52%)
 Frame = +3

Query: 162 GIVLGFLPVKFARENNLSNLVHPQGLVFLEPSSNYFR*WAHTHIQLWE 305
           G+VLG+  +K    + LS     + L+ + P + +   W H ++ +W+
Sbjct: 79  GVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHVNLYVWQ 126



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>RAD50_PYRAB (Q9UZC8) DNA double-strand break repair rad50 ATPase|
          Length = 880

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 26/96 (27%), Positives = 41/96 (42%), Gaps = 18/96 (18%)
 Frame = -3

Query: 335 KERMSISAFHLPKLDMSVGPLSEIVGGGLKKYKTL-------RVDEVAKVVFSSKLDGK- 180
           KE    +   + KL+ ++  + ++    +K YKTL       R+ E+A  +FS   DGK 
Sbjct: 704 KEEREKAKLEIKKLEKALSKVEDL-RKKIKDYKTLAKEQALNRISEIASEIFSEFTDGKY 762

Query: 179 ----------KTKDYAMLSV*VYKSKSLEQKFSSPG 102
                     KTK +      VY+ K +   F S G
Sbjct: 763 SNVIVRAEENKTKLFV-----VYEGKEVPLTFLSGG 793



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>ENGC_PSESM (Q87VI5) Probable GTPase engC (EC 3.6.1.-)|
          Length = 343

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -3

Query: 305 LPKLDMSVGPLSEIVGGGLKKYKTLRV 225
           LP+ D  VGPLSE+ G G     T R+
Sbjct: 231 LPETDTRVGPLSEVSGQGTHTTTTARL 257



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>SPT6H_DROME (Q9W420) Transcription elongation factor SPT6|
          Length = 1831

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 12/24 (50%), Positives = 16/24 (66%)
 Frame = +1

Query: 214  ATSSTLRVLYFLSPPPTISDSGPT 285
            A++S L  L+ + PPPTIS S  T
Sbjct: 1534 ASASNLTPLHLMRPPPTISSSSQT 1557



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>PCLO_MOUSE (Q9QYX7) Protein piccolo (Aczonin) (Multidomain presynaptic|
            cytomatrix protein) (Brain-derived HLMN protein)
          Length = 5038

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = -3

Query: 260  GGGLKKYKTLRVDEVAKVVFSSKLDGKKTKDY 165
            GGGL+++KT+ ++      +S++   KKT  Y
Sbjct: 1566 GGGLRRFKTIELNSTVTDKYSAESSQKKTTLY 1597



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>PCLO_HUMAN (Q9Y6V0) Protein piccolo (Aczonin)|
          Length = 5183

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = -3

Query: 260  GGGLKKYKTLRVDEVAKVVFSSKLDGKKTKDY 165
            GGGL+++KT+ ++      +S++   KKT  Y
Sbjct: 1592 GGGLRRFKTIELNSTIADKYSAESSQKKTSLY 1623



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>MEF2A_MOUSE (Q60929) Myocyte-specific enhancer factor 2A|
          Length = 498

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 14/26 (53%), Positives = 15/26 (57%)
 Frame = +1

Query: 184 PSSLLEKTTLATSSTLRVLYFLSPPP 261
           P S L   +LA SSTL     LSPPP
Sbjct: 157 PGSSLVSPSLAASSTLAETSMLSPPP 182



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>PCLO_RAT (Q9JKS6) Protein piccolo (Aczonin) (Multidomain presynaptic|
            cytomatrix protein)
          Length = 5085

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 11/29 (37%), Positives = 19/29 (65%)
 Frame = -3

Query: 260  GGGLKKYKTLRVDEVAKVVFSSKLDGKKT 174
            GGGL+++KT+ ++      +SS+   KKT
Sbjct: 1608 GGGLRRFKTIELNSTIADKYSSESSQKKT 1636


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,157,057
Number of Sequences: 219361
Number of extensions: 922261
Number of successful extensions: 2412
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 2390
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2411
length of database: 80,573,946
effective HSP length: 90
effective length of database: 60,831,456
effective search space used: 1459954944
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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