ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart42a10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MDAR2_ARATH (Q9LK94) Probable monodehydroascorbate reductase, cy... 54 1e-07
2CAPS1_HUMAN (Q9ULU8) Calcium-dependent secretion activator 1 (Ca... 34 0.16
3HIC1_MOUSE (Q9R1Y5) Hypermethylated in cancer 1 protein (Hic-1) 32 0.46
4CAPS1_RAT (Q62717) Calcium-dependent secretion activator 1 (Calc... 32 0.46
5CAPS1_MOUSE (Q80TJ1) Calcium-dependent secretion activator 1 (Ca... 32 0.46
6YAMP_RHOCA (P14172) Hypothetical 28.2 kDa protein in ampR 5'region 32 0.79
7MDAR3_ARATH (Q9LFA3) Probable monodehydroascorbate reductase, cy... 32 0.79
8MDAR_PEA (Q40977) Monodehydroascorbate reductase (EC 1.6.5.4) (M... 31 1.0
9CAPS1_XENLA (Q6GLR7) Calcium-dependent secretion activator 1 (Ca... 31 1.4
10CFAH_MOUSE (P06909) Complement factor H precursor (Protein beta-... 30 1.8
11COBQ_METTH (O26880) Probable cobyric acid synthase 30 1.8
12HEM3_CLOPE (Q8XKG4) Porphobilinogen deaminase (EC 2.5.1.61) (PBG... 30 2.3
13VE2_HPV28 (P50771) Regulatory protein E2 30 3.0
14ATPG_BARQU (Q6FYM2) ATP synthase gamma chain (EC 3.6.3.14) (ATP ... 30 3.0
15ATG9_YEAST (Q12142) Autophagy-related protein 9 (Cytoplasm to va... 29 3.9
16TARA_HUMAN (Q9H2D6) TRIO and F-actin-binding protein (Protein Ta... 29 5.1
17NRDJ_AGRT5 (Q8UEM4) Vitamin B12-dependent ribonucleotide reducta... 29 5.1
18MOBA_PYRAB (Q9V0D0) Probable molybdopterin-guanine dinucleotide ... 29 5.1
19PPE1_MOUSE (O35655) Serine/threonine-protein phosphatase with EF... 28 6.7
20KRAF1_DROME (P11346) Raf homolog serine/threonine-protein kinase... 28 6.7
21ARGN2_XENLA (Q91554) Arginase, non-hepatic 2 (EC 3.5.3.1) 28 8.8
22ARGN1_XENLA (Q91553) Arginase, non-hepatic 1 (EC 3.5.3.1) 28 8.8

>MDAR2_ARATH (Q9LK94) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 2 (EC 1.6.5.4) (MDAR 2)
          Length = 488

 Score = 54.3 bits (129), Expect = 1e-07
 Identities = 32/87 (36%), Positives = 48/87 (55%), Gaps = 15/87 (17%)
 Frame = -1

Query: 423 GGNRDDYEALSVVVRRKTKAS-DMSELERQGLAFA----IQESKKEVPD----------S 289
           GG +++YE +S   + K   + D+ ELER+GL FA     Q+   EV D          +
Sbjct: 391 GGTKEEYETISKATQLKPAVTIDLEELEREGLGFAHTVVSQQKVPEVKDIPSAEMVKQSA 450

Query: 288 GVTLGEKPTFVWYATAGVVAAVSISAF 208
            V + +KP +VW+A  GVV A S++AF
Sbjct: 451 SVVMIKKPLYVWHAATGVVVAASVAAF 477



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>CAPS1_HUMAN (Q9ULU8) Calcium-dependent secretion activator 1 (Calcium-dependent|
           activator protein for secretion 1) (CAPS-1)
          Length = 1353

 Score = 33.9 bits (76), Expect = 0.16
 Identities = 19/47 (40%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
 Frame = -3

Query: 232 CGSVDLRVRLLVRQEAPQVVKPGIGLRAAGINCWKE-GGRFIVYIQL 95
           C   DL+++L VR + PQ +K    L A G N WK    RF V +Q+
Sbjct: 503 CPDQDLKIKLAVRMDKPQNMKHSGYLWAIGKNVWKRWKKRFFVLVQV 549



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>HIC1_MOUSE (Q9R1Y5) Hypermethylated in cancer 1 protein (Hic-1)|
          Length = 892

 Score = 32.3 bits (72), Expect = 0.46
 Identities = 27/92 (29%), Positives = 33/92 (35%), Gaps = 5/92 (5%)
 Frame = -2

Query: 350 NLKDKA*RLPSRKARKRCLTAGLPSAKSPPSYGMQRQGSLRQCRSPRSVIGTAGSAAGGE 171
           NL+D      +    + C     P+A SP      R G    CR     I   G   G E
Sbjct: 66  NLQDLLSSASASLLAQVCARGRSPAAHSPRVAARWRHGRGSVCRFGPLQIRVCGKRGGAE 125

Query: 170 TR-----DRFAGGWDKLLEGRRKIYCLHTVTW 90
           TR     D  A   D+   GRR  +C H   W
Sbjct: 126 TRPGRGEDGPARQTDRGPGGRRAAHCSHVPPW 157



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>CAPS1_RAT (Q62717) Calcium-dependent secretion activator 1 (Calcium-dependent|
           activator protein for secretion 1) (CAPS-1) (rCAPS)
          Length = 1289

 Score = 32.3 bits (72), Expect = 0.46
 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = -3

Query: 232 CGSVDLRVRLLVRQEAPQVVKPGIGLRAAGINCWKE-GGRFIVYIQL 95
           C   DL+++L VR + PQ +K    L   G N WK    RF V +Q+
Sbjct: 501 CPDQDLKIKLAVRMDKPQNMKHSGYLWTIGKNVWKRWKKRFFVLVQV 547



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>CAPS1_MOUSE (Q80TJ1) Calcium-dependent secretion activator 1 (Calcium-dependent|
           activator protein for secretion 1) (CAPS-1)
          Length = 1355

 Score = 32.3 bits (72), Expect = 0.46
 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = -3

Query: 232 CGSVDLRVRLLVRQEAPQVVKPGIGLRAAGINCWKE-GGRFIVYIQL 95
           C   DL+++L VR + PQ +K    L   G N WK    RF V +Q+
Sbjct: 501 CPDQDLKIKLAVRMDKPQNMKHSGYLWTIGKNVWKRWKKRFFVLVQV 547



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>YAMP_RHOCA (P14172) Hypothetical 28.2 kDa protein in ampR 5'region|
          Length = 260

 Score = 31.6 bits (70), Expect = 0.79
 Identities = 20/62 (32%), Positives = 29/62 (46%)
 Frame = -2

Query: 395 CRWLSGVKPRPRTCLNLKDKA*RLPSRKARKRCLTAGLPSAKSPPSYGMQRQGSLRQCRS 216
           CR ++ +KP+    +   D A + P  +  K  +  G P     PS  + RQGSL  C  
Sbjct: 155 CRLMAAIKPKYTVRMTSCDVASQAPLAQILKGMVPCGSPL----PSCRVSRQGSLSACPW 210

Query: 215 PR 210
           PR
Sbjct: 211 PR 212



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>MDAR3_ARATH (Q9LFA3) Probable monodehydroascorbate reductase, cytoplasmic|
           isoform 3 (EC 1.6.5.4) (MDAR 3)
          Length = 434

 Score = 31.6 bits (70), Expect = 0.79
 Identities = 13/34 (38%), Positives = 23/34 (67%)
 Frame = -1

Query: 423 GGNRDDYEALSVVVRRKTKASDMSELERQGLAFA 322
           GG+ D+ +AL+ V + +  A  + EL +QG++FA
Sbjct: 398 GGSGDENKALAKVAKARPSAESLDELVKQGISFA 431



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>MDAR_PEA (Q40977) Monodehydroascorbate reductase (EC 1.6.5.4) (MDAR)|
           (Ascorbate free radical reductase) (AFR reductase)
          Length = 433

 Score = 31.2 bits (69), Expect = 1.0
 Identities = 13/34 (38%), Positives = 22/34 (64%)
 Frame = -1

Query: 423 GGNRDDYEALSVVVRRKTKASDMSELERQGLAFA 322
           GG  D+ +A++ V R K    D+++L  +GL+FA
Sbjct: 397 GGTPDENKAIAKVARAKPAVEDVNQLAEEGLSFA 430



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>CAPS1_XENLA (Q6GLR7) Calcium-dependent secretion activator 1 (Calcium-dependent|
           activator protein for secretion 1) (CAPS-1)
          Length = 1299

 Score = 30.8 bits (68), Expect = 1.4
 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = -3

Query: 232 CGSVDLRVRLLVRQEAPQVVKPGIGLRAAGINCWKE-GGRFIVYIQL 95
           C   DL+++L VR + PQ +K    L   G N WK    RF V +Q+
Sbjct: 470 CPDHDLKIKLAVRMDKPQNMKHCGYLWVIGKNVWKRWKKRFFVLVQV 516



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>CFAH_MOUSE (P06909) Complement factor H precursor (Protein beta-1-H)|
          Length = 1234

 Score = 30.4 bits (67), Expect = 1.8
 Identities = 15/43 (34%), Positives = 22/43 (51%)
 Frame = +3

Query: 294 QAPLSCFPGWQTLGLVFQVQTCPRPWFYAGQPPTMPRSRPGSH 422
           QA   C PG++TLG +  V+ C    + A  P  + R +P  H
Sbjct: 47  QATYKCRPGYRTLGTI--VKVCKNGKWVASNPSRICRKKPCGH 87



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>COBQ_METTH (O26880) Probable cobyric acid synthase|
          Length = 504

 Score = 30.4 bits (67), Expect = 1.8
 Identities = 17/50 (34%), Positives = 27/50 (54%)
 Frame = -1

Query: 423 GGNRDDYEALSVVVRRKTKASDMSELERQGLAFAIQESKKEVPDSGVTLG 274
           G + DD +A+ +   R T  SD+  LE  G A  I++  +E+P  G+  G
Sbjct: 286 GDSLDDLDAIIIPGTRNT-ISDLIHLEENGFADEIRDLSREIPVFGICGG 334



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>HEM3_CLOPE (Q8XKG4) Porphobilinogen deaminase (EC 2.5.1.61) (PBG)|
           (Hydroxymethylbilane synthase) (HMBS)
           (Pre-uroporphyrinogen synthase)
          Length = 291

 Score = 30.0 bits (66), Expect = 2.3
 Identities = 17/38 (44%), Positives = 20/38 (52%)
 Frame = -3

Query: 184 PQVVKPGIGLRAAGINCWKEGGRFIVYIQLLGGNPVQT 71
           P+V  P IG  A GI C K GG F  Y + L    V+T
Sbjct: 185 PKVFLPAIGQGALGIECLK-GGEFNDYFKALDSKEVRT 221



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>VE2_HPV28 (P50771) Regulatory protein E2|
          Length = 376

 Score = 29.6 bits (65), Expect = 3.0
 Identities = 12/37 (32%), Positives = 20/37 (54%)
 Frame = -2

Query: 140 KLLEGRRKIYCLHTVTWRKPCTNLYESVIVASCVNKY 30
           +L +G+RK+YC  + TWR  C +  ++  V      Y
Sbjct: 313 RLHKGKRKLYCKTSSTWRWSCESENQAAFVTIWYTSY 349



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>ATPG_BARQU (Q6FYM2) ATP synthase gamma chain (EC 3.6.3.14) (ATP synthase F1|
           sector gamma subunit)
          Length = 303

 Score = 29.6 bits (65), Expect = 3.0
 Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
 Frame = -3

Query: 289 RGYPRRKAHLRMVCNG-RGRCGSVDLRVRLLVRQEAPQVVKPG 164
           RG  R   HL +VC   RG CG+ ++++    RQ+   ++  G
Sbjct: 70  RGTGRNDVHLLVVCTAERGLCGAFNMQIARRARQQIKALLSAG 112



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>ATG9_YEAST (Q12142) Autophagy-related protein 9 (Cytoplasm to vacuole|
           targeting protein 7)
          Length = 997

 Score = 29.3 bits (64), Expect = 3.9
 Identities = 15/34 (44%), Positives = 19/34 (55%)
 Frame = +2

Query: 68  TGLYRVSSK*LYVNNKSSSFLPAVYPSRPQTDPG 169
           TG Y++ +K  Y NN+  SFL   Y  R Q  PG
Sbjct: 846 TGKYQLPAKKGYPNNEGDSFLNNKYSWRKQFQPG 879



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>TARA_HUMAN (Q9H2D6) TRIO and F-actin-binding protein (Protein Tara)|
            (Trio-associated repeat on actin)
          Length = 2365

 Score = 28.9 bits (63), Expect = 5.1
 Identities = 17/39 (43%), Positives = 19/39 (48%)
 Frame = -2

Query: 281  PSAKSPPSYGMQRQGSLRQCRSPRSVIGTAGSAAGGETR 165
            P A SPP +       L    SP S  G+ GSA  GETR
Sbjct: 1224 PRASSPPRHPPSDLAFLAPSPSPGSSGGSRGSAPPGETR 1262



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>NRDJ_AGRT5 (Q8UEM4) Vitamin B12-dependent ribonucleotide reductase (EC 1.17.4.1)|
            (Ribonucleoside-diphosphate reductase nrdJ)
          Length = 1272

 Score = 28.9 bits (63), Expect = 5.1
 Identities = 17/48 (35%), Positives = 22/48 (45%)
 Frame = -1

Query: 399  ALSVVVRRKTKASDMSELERQGLAFAIQESKKEVPDSGVTLGEKPTFV 256
            A+S + R      D S+     L   IQE K  +  +G T G KPT V
Sbjct: 1075 AVSYLGRHDLAHVDTSDFSNTALGKGIQEGKTNLLSTGWTRGYKPTLV 1122



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>MOBA_PYRAB (Q9V0D0) Probable molybdopterin-guanine dinucleotide biosynthesis|
           protein A
          Length = 191

 Score = 28.9 bits (63), Expect = 5.1
 Identities = 22/51 (43%), Positives = 32/51 (62%), Gaps = 1/51 (1%)
 Frame = -2

Query: 182 AGGETRDRFAGGWDKLL-EGRRKIYCLHTVTWRKPCTNLYESVIVASCVNK 33
           AGG++R RF  G DKLL E   K   LHT+   + C+++ + +IVAS  N+
Sbjct: 7   AGGKSR-RF--GEDKLLFEINGKPLVLHTIERLEGCSSIKKVIIVASPQNR 54



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>PPE1_MOUSE (O35655) Serine/threonine-protein phosphatase with EF-hands 1 (EC|
           3.1.3.16) (PPEF-1) (Protein phosphatase with EF
           calcium-binding domain) (PPEF) (DRES10) (Fragment)
          Length = 270

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 16/35 (45%), Positives = 19/35 (54%)
 Frame = +1

Query: 190 PAVPITERGDRHCRNDPCRCIPYEGGLFAEGNPAV 294
           PA  IT  GD H + D    I Y+ GL +E NP V
Sbjct: 182 PAKEITICGDLHGKLDDLMLIFYKNGLPSENNPYV 216



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>KRAF1_DROME (P11346) Raf homolog serine/threonine-protein kinase dRAF-1 (EC|
           2.7.11.1) (Protein pole-hole)
          Length = 739

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 15/39 (38%), Positives = 21/39 (53%)
 Frame = -2

Query: 326 LPSRKARKRCLTAGLPSAKSPPSYGMQRQGSLRQCRSPR 210
           + SR   +RC ++G  S+  PPS      G+ RQ R PR
Sbjct: 302 MSSRSRSRRCSSSGSSSSSKPPS---SSSGNHRQGRPPR 337



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>ARGN2_XENLA (Q91554) Arginase, non-hepatic 2 (EC 3.5.3.1)|
          Length = 360

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 21/56 (37%), Positives = 30/56 (53%), Gaps = 6/56 (10%)
 Frame = +2

Query: 209 NAEIDTAATTPAVAYHTK-VGF-----SPRVTPLSGTSFLLSWMANARPCLSSSDM 358
           +A+I+T  TTP+   H + V F       +V P+ G     SW   A+PCLS SD+
Sbjct: 147 HADINTPLTTPSGNLHGQPVSFLLRELQDKVPPIPG----FSW---AKPCLSKSDI 195



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>ARGN1_XENLA (Q91553) Arginase, non-hepatic 1 (EC 3.5.3.1)|
          Length = 360

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 21/56 (37%), Positives = 30/56 (53%), Gaps = 6/56 (10%)
 Frame = +2

Query: 209 NAEIDTAATTPAVAYHTK-VGF-----SPRVTPLSGTSFLLSWMANARPCLSSSDM 358
           +A+I+T  TTP+   H + V F       +V P+ G     SW   A+PCLS SD+
Sbjct: 147 HADINTPLTTPSGNLHGQPVSFLLRELQDKVPPIPG----FSW---AKPCLSKSDI 195


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,811,208
Number of Sequences: 219361
Number of extensions: 1574069
Number of successful extensions: 4345
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 4214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4343
length of database: 80,573,946
effective HSP length: 101
effective length of database: 58,418,485
effective search space used: 2278320915
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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