ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart38g09
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RHGA_ASPAC (Q00001) Rhamnogalacturonase A precursor (EC 3.2.1.-)... 44 2e-04
2PGLR2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.... 39 0.006
3PGLR_LYCES (P05117) Polygalacturonase-2 precursor (EC 3.2.1.15) ... 39 0.006
4PGLR2_ARATH (P49063) Exopolygalacturonase clone GBGA483 precurso... 38 0.013
5PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (P... 37 0.029
6PGLR2_CHAOB (Q7M1E7) Polygalacturonase precursor (PG) (EC 3.2.1.... 36 0.065
7PGLR2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (... 35 0.14
8PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (P... 33 0.32
9PGLR3_MAIZE (P35339) Exopolygalacturonase precursor (EC 3.2.1.67... 32 0.72
10PGLR_OENOR (P24548) Exopolygalacturonase (EC 3.2.1.67) (ExoPG) (... 31 2.1
11PGLR_MEDSA (Q40312) Polygalacturonase precursor (EC 3.2.1.15) (P... 31 2.1
12PGLR1_MAIZE (P26216) Exopolygalacturonase precursor (EC 3.2.1.67... 30 2.7
13SRF_XENLA (P23790) Serum response factor (SRF) 30 3.6
14PGLR2_MAIZE (P35338) Exopolygalacturonase precursor (EC 3.2.1.67... 29 6.1
15PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (P... 29 6.1

>RHGA_ASPAC (Q00001) Rhamnogalacturonase A precursor (EC 3.2.1.-) (RGase A)|
           (RHG A)
          Length = 440

 Score = 43.9 bits (102), Expect = 2e-04
 Identities = 25/84 (29%), Positives = 38/84 (45%), Gaps = 4/84 (4%)
 Frame = -2

Query: 490 IKLACSDAVPCSDIVLSNINLLGDDGAEVQAVCNCAMGLGYDPVRPAVDCLRNNACXXXX 311
           I++ CSD  PC+D+ L +I +  + G+    +C  A G GY        CL++++     
Sbjct: 336 IRVVCSDTAPCTDLTLEDIAIWTESGSSELYLCRSAYGSGY--------CLKDSSSHTSY 387

Query: 310 XXXXXXXXEPS----TTTAAPLHT 251
                    PS    TT AA L T
Sbjct: 388 TTTSTVTAAPSGYSATTMAADLAT 411



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>PGLR2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.15) (Pectinase)|
           (Major pollen allergen Jun a 2)
          Length = 507

 Score = 39.3 bits (90), Expect = 0.006
 Identities = 22/52 (42%), Positives = 29/52 (55%)
 Frame = -2

Query: 493 AIKLACSDAVPCSDIVLSNINLLGDDGAEVQAVCNCAMGLGYDPVRPAVDCL 338
           AI+L CSD+VPCS+I LSN+ L    G     V   A G   +P+ P+   L
Sbjct: 380 AIQLMCSDSVPCSNIKLSNVFLKLTSGKVATCVNKNANGYYTNPLNPSCKSL 431



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>PGLR_LYCES (P05117) Polygalacturonase-2 precursor (EC 3.2.1.15) (PG) (PG-2A)|
           (PG-2B) (Pectinase)
          Length = 457

 Score = 39.3 bits (90), Expect = 0.006
 Identities = 16/34 (47%), Positives = 21/34 (61%)
 Frame = -2

Query: 493 AIKLACSDAVPCSDIVLSNINLLGDDGAEVQAVC 392
           AIK  CS   PC  I++ NINL+G+ G   +A C
Sbjct: 394 AIKFDCSTNFPCEGIIMENINLVGESGKPSEATC 427



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>PGLR2_ARATH (P49063) Exopolygalacturonase clone GBGA483 precursor (EC 3.2.1.67)|
           (ExoPG) (Pectinase) (Galacturan
           1,4-alpha-galacturonidase)
          Length = 444

 Score = 38.1 bits (87), Expect = 0.013
 Identities = 19/37 (51%), Positives = 25/37 (67%), Gaps = 2/37 (5%)
 Frame = -2

Query: 493 AIKLACSDAVPCSDIVLSNINLL--GDDGAEVQAVCN 389
           A+KL CS  VPC++I LS+INL+  G +G  V A  N
Sbjct: 386 AVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSN 422



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>PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)|
          Length = 462

 Score = 37.0 bits (84), Expect = 0.029
 Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = -2

Query: 493 AIKLACSDAVPCSDIVLSNINLLGDDGAEVQAVC-NCAMGL 374
           A+K  CS + PC   ++ NINL+G+ G E    C N   GL
Sbjct: 402 AVKFDCSKSSPCQGYIVGNINLVGNGGKETTMSCSNIVQGL 442



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>PGLR2_CHAOB (Q7M1E7) Polygalacturonase precursor (PG) (EC 3.2.1.15) (Pectinase)|
           (Major pollen allergen Cha o 2)
          Length = 514

 Score = 35.8 bits (81), Expect = 0.065
 Identities = 21/53 (39%), Positives = 27/53 (50%)
 Frame = -2

Query: 493 AIKLACSDAVPCSDIVLSNINLLGDDGAEVQAVCNCAMGLGYDPVRPAVDCLR 335
           AI+L CSD+VPC+ I LSN++L    G     V   A G     + P    LR
Sbjct: 379 AIQLMCSDSVPCTGIQLSNVSLKLTSGKPASCVDKNARGFYSGRLIPTCKNLR 431



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>PGLR2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)|
           (Major pollen allergen Cry j 2) (Cry j II)
          Length = 514

 Score = 34.7 bits (78), Expect = 0.14
 Identities = 22/56 (39%), Positives = 30/56 (53%)
 Frame = -2

Query: 493 AIKLACSDAVPCSDIVLSNINLLGDDGAEVQAVCNCAMGLGYDPVRPAVDCLRNNA 326
           AI+L CSD++PC DI LS+I+L    G     + + A G     V PA   L  +A
Sbjct: 379 AIQLKCSDSMPCKDIKLSDISLKLTSGKIASCLNDNANGYFSGHVIPACKNLSPSA 434



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>PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)|
          Length = 467

 Score = 33.5 bits (75), Expect = 0.32
 Identities = 15/35 (42%), Positives = 21/35 (60%)
 Frame = -2

Query: 493 AIKLACSDAVPCSDIVLSNINLLGDDGAEVQAVCN 389
           AI   CS   PC  IVL +++L  + GA  +A+CN
Sbjct: 407 AITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCN 441



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>PGLR3_MAIZE (P35339) Exopolygalacturonase precursor (EC 3.2.1.67) (ExoPG)|
           (Pectinase) (Galacturan 1,4-alpha-galacturonidase)
          Length = 410

 Score = 32.3 bits (72), Expect = 0.72
 Identities = 17/59 (28%), Positives = 28/59 (47%), Gaps = 1/59 (1%)
 Frame = -2

Query: 496 EAIKLACSDAVPCSDIVLSNINL-LGDDGAEVQAVCNCAMGLGYDPVRPAVDCLRNNAC 323
           EA+ L C+  +PC+ + + ++N+       +  AVC  A G        A  CL+  AC
Sbjct: 358 EAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKG-------SAKGCLKELAC 409



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>PGLR_OENOR (P24548) Exopolygalacturonase (EC 3.2.1.67) (ExoPG) (Pectinase)|
           (Galacturan 1,4-alpha-galacturonidase) (Fragment)
          Length = 362

 Score = 30.8 bits (68), Expect = 2.1
 Identities = 14/36 (38%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
 Frame = -2

Query: 496 EAIKLACSDAVPCSDIVLSNINL-LGDDGAEVQAVC 392
           EA+KL CS + PC+ + L++I+L     G    +VC
Sbjct: 303 EAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVC 338



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>PGLR_MEDSA (Q40312) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)|
          Length = 421

 Score = 30.8 bits (68), Expect = 2.1
 Identities = 15/35 (42%), Positives = 21/35 (60%)
 Frame = -2

Query: 496 EAIKLACSDAVPCSDIVLSNINLLGDDGAEVQAVC 392
           E + L CS AVPC  + L+N++ L  +GA   A C
Sbjct: 344 EGVVLICSSAVPCDGVELNNVD-LKFNGAPTTAKC 377



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>PGLR1_MAIZE (P26216) Exopolygalacturonase precursor (EC 3.2.1.67) (ExoPG)|
           (Pectinase) (Galacturan 1,4-alpha-galacturonidase)
          Length = 410

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 16/59 (27%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
 Frame = -2

Query: 496 EAIKLACSDAVPCSDIVLSNINL-LGDDGAEVQAVCNCAMGLGYDPVRPAVDCLRNNAC 323
           EA+ L C+  VPC+ + + ++N+       +  A+C  A G           CL+  AC
Sbjct: 358 EAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNAKG-------STKGCLKELAC 409



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>SRF_XENLA (P23790) Serum response factor (SRF)|
          Length = 448

 Score = 30.0 bits (66), Expect = 3.6
 Identities = 17/42 (40%), Positives = 25/42 (59%)
 Frame = +3

Query: 372 PSPMAQLHTACTSAPSSPSRLMLLSTMSLHGTASLQASLMAS 497
           P    Q+H+A  ++PSS S   L+ T S  GT +L A++M S
Sbjct: 301 PVQAIQVHSAAQASPSSDSSSELVQTSS-SGTVTLPAAIMTS 341



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>PGLR2_MAIZE (P35338) Exopolygalacturonase precursor (EC 3.2.1.67) (ExoPG)|
           (Pectinase) (Galacturan 1,4-alpha-galacturonidase)
          Length = 410

 Score = 29.3 bits (64), Expect = 6.1
 Identities = 17/59 (28%), Positives = 26/59 (44%), Gaps = 1/59 (1%)
 Frame = -2

Query: 496 EAIKLACSDAVPCSDIVLSNINL-LGDDGAEVQAVCNCAMGLGYDPVRPAVDCLRNNAC 323
           EAI L C+  VPC+   + ++N+       +  A+C  A G           CL+  AC
Sbjct: 358 EAISLLCTAKVPCTGATMDDVNVEYSGTNNKTMAICTNAKG-------STKGCLKELAC 409



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>PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (PG) (Pectinase)|
          Length = 460

 Score = 29.3 bits (64), Expect = 6.1
 Identities = 12/23 (52%), Positives = 16/23 (69%)
 Frame = -2

Query: 496 EAIKLACSDAVPCSDIVLSNINL 428
           +AI L CS +VPC  IVL ++ L
Sbjct: 416 DAITLNCSQSVPCQGIVLQSVQL 438


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,225,943
Number of Sequences: 219361
Number of extensions: 781970
Number of successful extensions: 2175
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 2134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2175
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3523384522
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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