ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart36g08
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TMBI1_HUMAN (Q969X1) Transmembrane BAX inhibitor motif-containin... 30 1.2
2ACTN1_HUMAN (P12814) Alpha-actinin-1 (Alpha-actinin cytoskeletal... 30 2.0
3VP13B_MOUSE (Q80TY5) Vacuolar protein sorting 13B (Cohen syndrom... 29 2.6
4ACTN1_CHICK (P05094) Alpha-actinin-1 (Alpha-actinin cytoskeletal... 29 3.4
5CYB_LEITA (P14548) Cytochrome b 29 3.4
6PK1L1_MOUSE (Q8R526) Polycystic kidney disease 1-like 1 protein ... 29 3.4
7VP13B_HUMAN (Q7Z7G8) Vacuolar protein sorting 13B (Cohen syndrom... 29 3.4
8ESCO2_MOUSE (Q8CIB9) N-acetyltransferase ESCO2 (EC 2.3.1.-) (Est... 28 4.5
9Y316_MYCPN (P75465) Hypothetical protein MG223 homolog (F10_orf419) 28 4.5
10Y1645_METJA (Q59039) Hypothetical protein MJ1645 28 5.8
11COBD_LISIN (Q92CL6) Cobalamin biosynthesis protein cobD 28 5.8
12ACTN1_RAT (Q9Z1P2) Alpha-actinin-1 (Alpha-actinin cytoskeletal i... 28 5.8
13ACTN1_MOUSE (Q7TPR4) Alpha-actinin-1 (Alpha-actinin cytoskeletal... 28 5.8
14LGR8_CANFA (Q5XM32) Relaxin receptor 2 (Leucine-rich repeat-cont... 28 5.8
15XCT_HUMAN (Q9UPY5) Cystine/glutamate transporter (Amino acid tra... 28 5.8
16CIN8_YEAST (P27895) Kinesin-like protein CIN8 (Chromosome instab... 28 7.6
17IF2_ENTFC (P18311) Translation initiation factor IF-2 28 7.6
18ROK1_EMENI (Q5BET6) ATP-dependent RNA helicase rok1 (EC 3.6.1.-) 27 10.0
19NU4C_ADICA (Q85FH5) NAD(P)H-quinone oxidoreductase chain 4, chlo... 27 10.0
20PER3_HUMAN (P56645) Period circadian protein 3 (hPER3) 27 10.0
211A12_CUCMA (Q00257) 1-aminocyclopropane-1-carboxylate synthase C... 27 10.0

>TMBI1_HUMAN (Q969X1) Transmembrane BAX inhibitor motif-containing protein 1|
           (RECS1 protein homolog)
          Length = 311

 Score = 30.4 bits (67), Expect = 1.2
 Identities = 15/39 (38%), Positives = 22/39 (56%)
 Frame = -3

Query: 232 GLF*LLALVYIGFLVVGLCRLFVVAFPFYYWLQLLYIGI 116
           GLF +L +V    LV G+    V+ F + YWL +LY  +
Sbjct: 221 GLFCVLGIV---LLVTGIVTSIVLYFQYVYWLHMLYAAL 256



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>ACTN1_HUMAN (P12814) Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform)|
           (Non-muscle alpha-actinin-1) (F-actin cross linking
           protein)
          Length = 892

 Score = 29.6 bits (65), Expect = 2.0
 Identities = 13/44 (29%), Positives = 21/44 (47%)
 Frame = +2

Query: 83  NTTWGTYREIQNAYVQELKPIIKRKSHYKQSAQTNNQKAYVHES 214
           N  WG   +++  Y + L   I+R       A+   QKA +HE+
Sbjct: 365 NNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEA 408



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>VP13B_MOUSE (Q80TY5) Vacuolar protein sorting 13B (Cohen syndrome protein 1|
           homolog) (Fragment)
          Length = 1603

 Score = 29.3 bits (64), Expect = 2.6
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -2

Query: 224 LVACSRVHRLFGCWFVQIVCSGFSF 150
           L AC+RV   F  WFV  +C  F F
Sbjct: 44  LAACTRVDSCFTPWFVPSLCMSFQF 68



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>ACTN1_CHICK (P05094) Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform)|
           (Non-muscle alpha-actinin-1) (F-actin cross linking
           protein)
          Length = 893

 Score = 28.9 bits (63), Expect = 3.4
 Identities = 14/44 (31%), Positives = 20/44 (45%)
 Frame = +2

Query: 83  NTTWGTYREIQNAYVQELKPIIKRKSHYKQSAQTNNQKAYVHES 214
           N  WG   + +  Y + L   I+R       A+   QKA +HES
Sbjct: 366 NNAWGGLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHES 409



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>CYB_LEITA (P14548) Cytochrome b|
          Length = 371

 Score = 28.9 bits (63), Expect = 3.4
 Identities = 22/84 (26%), Positives = 33/84 (39%), Gaps = 12/84 (14%)
 Frame = -1

Query: 267 CLVCLF*KKLELAFFSCLLSCT*AFWLLVCADCL*WLF------------LFIIGFNSCT 124
           CL+ ++     L FF C+         ++C  CL WLF            LF+  F+   
Sbjct: 25  CLLRVYGVGFSLGFFICMQ--------IICGVCLAWLFFSCFICTNWYFVLFLWDFDLGF 76

Query: 123 *AFWISLYVPHVVFFYLYAHSFIC 52
                 +    ++FF LY H F C
Sbjct: 77  VIRSTHICFTSLLFFLLYVHIFKC 100



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>PK1L1_MOUSE (Q8R526) Polycystic kidney disease 1-like 1 protein|
           (Polycystin-1L1) (Fragment)
          Length = 531

 Score = 28.9 bits (63), Expect = 3.4
 Identities = 18/77 (23%), Positives = 33/77 (42%), Gaps = 4/77 (5%)
 Frame = -2

Query: 260 CVCFKKS*SWPFL----VACSRVHRLFGCWFVQIVCSGFSFLLLASTPVHRHFGFPCMSP 93
           C+ +  S +W       +AC       G WFV   C  + +LLL             +S 
Sbjct: 402 CLLWSSSVAWAISGSASLACGLGTGFLGYWFVPAQCMWWLYLLL-------------LSL 448

Query: 92  MSCSFICMPILLFVVSM 42
           + C+FI  P+++ + ++
Sbjct: 449 VCCAFITQPLMICLAAL 465



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>VP13B_HUMAN (Q7Z7G8) Vacuolar protein sorting 13B (Cohen syndrome protein 1)|
          Length = 4022

 Score = 28.9 bits (63), Expect = 3.4
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -2

Query: 224  LVACSRVHRLFGCWFVQIVCSGFSF 150
            L AC+RV   F  WFV  +C  F F
Sbjct: 2461 LAACTRVDSCFTPWFVPSLCVSFQF 2485



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>ESCO2_MOUSE (Q8CIB9) N-acetyltransferase ESCO2 (EC 2.3.1.-) (Establishment of|
           cohesion 1 homolog 2) (ECO1 homolog 2)
          Length = 592

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 18/67 (26%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
 Frame = +1

Query: 115 KCLCTGVEANNKKEKPLQTICTNQQPKSLCTREQATKKGQL*LFLKQTHQAILSKEKG-K 291
           + +C   E+    +KP+ ++  N Q K +CT++   K+  L    +  ++ I SK +  K
Sbjct: 116 RSICLATESG---DKPIPSVTENIQRKPVCTKKNKKKQKSLTAKYQPNYKHIKSKSRNLK 172

Query: 292 NEAMNIV 312
           N   N V
Sbjct: 173 NSKPNQV 179



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>Y316_MYCPN (P75465) Hypothetical protein MG223 homolog (F10_orf419)|
          Length = 419

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 14/43 (32%), Positives = 20/43 (46%)
 Frame = +2

Query: 101 YREIQNAYVQELKPIIKRKSHYKQSAQTNNQKAYVHESKQLKK 229
           Y   +N+ V+ELKP +KR    K      +     H  KQ+ K
Sbjct: 160 YYTTKNSLVKELKPFLKRNIIVKIDKIVTHHMVLAHSIKQINK 202



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>Y1645_METJA (Q59039) Hypothetical protein MJ1645|
          Length = 243

 Score = 28.1 bits (61), Expect = 5.8
 Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 6/75 (8%)
 Frame = +2

Query: 23  SGNLQVAYLQQIKEWAYK*KNTTWGTYREIQNAYVQELKPI------IKRKSHYKQSAQT 184
           +G   + Y +QIKE A   K+  + T+ E    + + +         IK K H++     
Sbjct: 84  TGGEPLLYHKQIKEIAEILKDKGYRTFLESNGMFPERIFYFDIASIDIKLKEHFEYIKDE 143

Query: 185 NNQKAYVHESKQLKK 229
           + +K Y +E K +KK
Sbjct: 144 DYEKLYKNELKTIKK 158



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>COBD_LISIN (Q92CL6) Cobalamin biosynthesis protein cobD|
          Length = 315

 Score = 28.1 bits (61), Expect = 5.8
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = -1

Query: 159 LFLFIIGFNSCT*AFWISLYVPHVVFFYLYAHSFICCKYAT 37
           LF+  +G        W+ LY+   + F+LYA  FI   Y T
Sbjct: 56  LFILTVGMTGIV--SWLILYISAKIAFWLYAAVFIYLGYTT 94



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>ACTN1_RAT (Q9Z1P2) Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform)|
           (Non-muscle alpha-actinin-1) (F-actin cross linking
           protein)
          Length = 892

 Score = 28.1 bits (61), Expect = 5.8
 Identities = 13/44 (29%), Positives = 20/44 (45%)
 Frame = +2

Query: 83  NTTWGTYREIQNAYVQELKPIIKRKSHYKQSAQTNNQKAYVHES 214
           N  WG   + +  Y + L   I+R       A+   QKA +HE+
Sbjct: 365 NNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEA 408



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>ACTN1_MOUSE (Q7TPR4) Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform)|
           (Non-muscle alpha-actinin-1) (F-actin cross linking
           protein)
          Length = 892

 Score = 28.1 bits (61), Expect = 5.8
 Identities = 13/44 (29%), Positives = 20/44 (45%)
 Frame = +2

Query: 83  NTTWGTYREIQNAYVQELKPIIKRKSHYKQSAQTNNQKAYVHES 214
           N  WG   + +  Y + L   I+R       A+   QKA +HE+
Sbjct: 365 NNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEA 408



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>LGR8_CANFA (Q5XM32) Relaxin receptor 2 (Leucine-rich repeat-containing|
           G-protein coupled receptor 8) (INSL3 receptor)
          Length = 737

 Score = 28.1 bits (61), Expect = 5.8
 Identities = 11/18 (61%), Positives = 14/18 (77%)
 Frame = -1

Query: 189 LLVCADCL*WLFLFIIGF 136
           +L CADCL  ++LF IGF
Sbjct: 439 ILCCADCLMGVYLFFIGF 456



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>XCT_HUMAN (Q9UPY5) Cystine/glutamate transporter (Amino acid transport system|
           xc-) (xCT) (Calcium channel blocker resistance protein
           CCBR1)
          Length = 501

 Score = 28.1 bits (61), Expect = 5.8
 Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = -2

Query: 194 FGCW-FVQIVCSGFSFLLLASTPVHRHFGFPCMSPMSCSFICMPILLFVVSMQL 36
           F  W F+ +  +G  +L      +HR F  P   P   SF C    LF+V++ L
Sbjct: 394 FARWLFIGLAVAGLIYLRYKCPDMHRPFKVPLFIPALFSFTC----LFMVALSL 443



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>CIN8_YEAST (P27895) Kinesin-like protein CIN8 (Chromosome instability protein|
           8)
          Length = 1000

 Score = 27.7 bits (60), Expect = 7.6
 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
 Frame = +2

Query: 116 NAYVQELKPIIKRKSH-YKQSAQTNNQKAYVHESKQLKKASSNFF 247
           N  +Q+L  +++   H YK+     NQK  +H +K++KK  S  F
Sbjct: 600 NNKLQKLTEVMQMALHDYKKRELDLNQKFEMHITKEIKKLKSTLF 644



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>IF2_ENTFC (P18311) Translation initiation factor IF-2|
          Length = 784

 Score = 27.7 bits (60), Expect = 7.6
 Identities = 25/97 (25%), Positives = 42/97 (43%), Gaps = 4/97 (4%)
 Frame = +1

Query: 28  KPSSCILTTNKRMGIQIKEHDMGDI-QGNPKCLCTGVEANNKKEKPLQTIC---TNQQPK 195
           +PS  ++   +++GI +K H MG I  G+ K L    +     +KP Q         QP 
Sbjct: 15  QPSKDVVEKAQQLGINVKNH-MGTITTGDEKKLQQAFKKPQTNKKPAQQASQKPATNQPN 73

Query: 196 SLCTREQATKKGQL*LFLKQTHQAILSKEKGKNEAMN 306
               +E  TK+ +     +   Q      +GKN++ N
Sbjct: 74  EQKKQETKTKQQKNNRNYQDRGQGSGQVNQGKNQSTN 110



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>ROK1_EMENI (Q5BET6) ATP-dependent RNA helicase rok1 (EC 3.6.1.-)|
          Length = 742

 Score = 27.3 bits (59), Expect = 10.0
 Identities = 15/48 (31%), Positives = 23/48 (47%)
 Frame = +1

Query: 73  QIKEHDMGDIQGNPKCLCTGVEANNKKEKPLQTICTNQQPKSLCTREQ 216
           +IK  DM D++          E   KK++  Q     QQP++L  +EQ
Sbjct: 126 KIKVTDMRDLEELAPAQVQSEEPKKKKKRKQQEEAEKQQPQTLSKKEQ 173



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>NU4C_ADICA (Q85FH5) NAD(P)H-quinone oxidoreductase chain 4, chloroplast (EC|
           1.6.5.-) (NAD(P)H dehydrogenase, chain 4)
           (NADH-plastoquinone oxidoreductase chain 4)
          Length = 497

 Score = 27.3 bits (59), Expect = 10.0
 Identities = 13/28 (46%), Positives = 18/28 (64%)
 Frame = -3

Query: 220 LLALVYIGFLVVGLCRLFVVAFPFYYWL 137
           L  L+Y+GFLV    +L +  FPF+ WL
Sbjct: 210 LEVLIYVGFLVAYAVKLPI--FPFHTWL 235



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>PER3_HUMAN (P56645) Period circadian protein 3 (hPER3)|
          Length = 1201

 Score = 27.3 bits (59), Expect = 10.0
 Identities = 13/33 (39%), Positives = 16/33 (48%)
 Frame = -3

Query: 160 AFPFYYWLQLLYIGILDFPVCPPCRVLLFVCPF 62
           AFPF Y    + + + D PVCP        CPF
Sbjct: 838 AFPFPYLDTFMTVFLPDPPVCPLLSPSFLPCPF 870



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>1A12_CUCMA (Q00257) 1-aminocyclopropane-1-carboxylate synthase CMA101 (EC|
           4.4.1.14) (ACC synthase) (S-adenosyl-L-methionine
           methylthioadenosine-lyase)
          Length = 475

 Score = 27.3 bits (59), Expect = 10.0
 Identities = 14/50 (28%), Positives = 28/50 (56%)
 Frame = +2

Query: 47  LQQIKEWAYK*KNTTWGTYREIQNAYVQELKPIIKRKSHYKQSAQTNNQK 196
           ++++K +  + ++TT   +R   N   + +K  I  K  ++QSAQ  N+K
Sbjct: 421 VRRLKSFVTELRSTTTSNHRNHDNKICKNIKKNIFTKWVFRQSAQEANRK 470


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,648,891
Number of Sequences: 219361
Number of extensions: 974151
Number of successful extensions: 2732
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 2683
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2729
length of database: 80,573,946
effective HSP length: 79
effective length of database: 63,244,427
effective search space used: 1517866248
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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