ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart35b08
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1VPS4B_HUMAN (O75351) Vacuolar sorting protein 4b (SKD1 protein) 39 0.006
2VPS4A_MOUSE (Q8VEJ9) Vacuolar sorting protein 4a 39 0.010
3VPS4A_HUMAN (Q9UN37) Vacuolar sorting protein 4a (SKD2 protein) ... 38 0.017
4VPS4B_MOUSE (P46467) Vacuolar sorting protein 4b (SKD1 protein) 37 0.029
5CEMA_CHAGL (Q8M9X3) Chloroplast envelope membrane protein 34 0.24
6VPS4_YEAST (P52917) Vacuolar protein sorting-associated protein ... 32 0.71
7PTK6_HUMAN (Q13882) Tyrosine-protein kinase 6 (EC 2.7.10.2) (Bre... 32 0.71
8ZNHI4_MOUSE (Q99PT3) Zinc finger HIT domain-containing protein 4... 30 2.7
9ECLC_BOVIN (P54281) Epithelial chloride channel protein (Calcium... 30 2.7
10MASS1_MOUSE (Q8VHN7) Monogenic audiogenic seizure susceptibility... 30 2.7
11DYN1_HUMAN (Q05193) Dynamin-1 (EC 3.6.5.5) 30 4.6
12BAG3_MOUSE (Q9JLV1) BAG family molecular chaperone regulator 3 (... 29 6.0
13TOM40_MOUSE (Q9QYA2) Probable mitochondrial import receptor subu... 29 6.0
14VPS4_SCHPO (Q09803) Suppressor protein of bem1/bed5 double mutants 29 7.8
15WIRE_MOUSE (Q6PEV3) WIP-related protein (WASP-interacting protei... 29 7.8
16WIRE_HUMAN (Q8TF74) WIP-related protein (WASP-interacting protei... 29 7.8
17PHO4_NEUCR (P15710) Phosphate-repressible phosphate permease 29 7.8
18CEMA_MARPO (P12211) Chloroplast envelope membrane protein 29 7.8

>VPS4B_HUMAN (O75351) Vacuolar sorting protein 4b (SKD1 protein)|
          Length = 444

 Score = 39.3 bits (90), Expect = 0.006
 Identities = 19/56 (33%), Positives = 30/56 (53%)
 Frame = -1

Query: 478 DMWMPCGSKQPGAVQTTMQDLASKGLASQILPPPISKSDFEKVLARQRPTVGKKDL 311
           D+  PC    PGA++ T  D+       ++L P +S SD  + L+  +PTV + DL
Sbjct: 379 DLLTPCSPGDPGAIEMTWMDVP----GDKLLEPVVSMSDMLRSLSNTKPTVNEHDL 430



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>VPS4A_MOUSE (Q8VEJ9) Vacuolar sorting protein 4a|
          Length = 437

 Score = 38.5 bits (88), Expect = 0.010
 Identities = 20/56 (35%), Positives = 28/56 (50%)
 Frame = -1

Query: 478 DMWMPCGSKQPGAVQTTMQDLASKGLASQILPPPISKSDFEKVLARQRPTVGKKDL 311
           D+  PC    PGA++ T  D+       ++L P +  SD  + LA  RPTV   DL
Sbjct: 372 DLLTPCSPGDPGAIEMTWMDVP----GDKLLEPVVCMSDMLRSLATTRPTVNADDL 423



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>VPS4A_HUMAN (Q9UN37) Vacuolar sorting protein 4a (SKD2 protein) (hVPS4)|
           (VPS4-1)
          Length = 437

 Score = 37.7 bits (86), Expect = 0.017
 Identities = 20/56 (35%), Positives = 28/56 (50%)
 Frame = -1

Query: 478 DMWMPCGSKQPGAVQTTMQDLASKGLASQILPPPISKSDFEKVLARQRPTVGKKDL 311
           D+  PC    PGA++ T  D+       ++L P +  SD  + LA  RPTV   DL
Sbjct: 372 DLLTPCSPGDPGAMEMTWMDVP----GDKLLEPVVCMSDMLRSLATTRPTVNADDL 423



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>VPS4B_MOUSE (P46467) Vacuolar sorting protein 4b (SKD1 protein)|
          Length = 444

 Score = 37.0 bits (84), Expect = 0.029
 Identities = 18/56 (32%), Positives = 30/56 (53%)
 Frame = -1

Query: 478 DMWMPCGSKQPGAVQTTMQDLASKGLASQILPPPISKSDFEKVLARQRPTVGKKDL 311
           D+  PC    PGA++ T  D+       ++L P +S  D  + L+  +PTV ++DL
Sbjct: 379 DLLTPCSPGDPGAIEMTWMDVP----GDKLLEPVVSMWDMLRSLSSTKPTVNEQDL 430



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>CEMA_CHAGL (Q8M9X3) Chloroplast envelope membrane protein|
          Length = 472

 Score = 33.9 bits (76), Expect = 0.24
 Identities = 26/87 (29%), Positives = 40/87 (45%), Gaps = 12/87 (13%)
 Frame = -3

Query: 257 QPRELSRPMHSRTTEMYIVYNFDS----LRLGVKFADLYTLVA--------LTVLNVWRR 114
           QP++L   +H RT ++   YN DS    L L   F  ++TL+         L +LN W  
Sbjct: 323 QPQDLGALIHQRTIQLAEYYNQDSINIILHLFTDFIAIFTLIVLLILGKKRLAILNSW-- 380

Query: 113 RQPCYAGLSLCLCCQLYFTYTLLCLEF 33
            Q  +  L+  +   L   +T LC+ F
Sbjct: 381 VQEVFYSLTDTMKAFLILLFTDLCIGF 407



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>VPS4_YEAST (P52917) Vacuolar protein sorting-associated protein VPS4 (END13|
           protein) (DOA4-independent degradation protein 6)
          Length = 437

 Score = 32.3 bits (72), Expect = 0.71
 Identities = 16/52 (30%), Positives = 26/52 (50%)
 Frame = -1

Query: 466 PCGSKQPGAVQTTMQDLASKGLASQILPPPISKSDFEKVLARQRPTVGKKDL 311
           PC     GA++ +  D+     A ++  P ++  DF K +   RPTV + DL
Sbjct: 375 PCSPGDDGAIEMSWTDIE----ADELKEPDLTIKDFLKAIKSTRPTVNEDDL 422



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>PTK6_HUMAN (Q13882) Tyrosine-protein kinase 6 (EC 2.7.10.2) (Breast tumor|
           kinase) (Tyrosine-protein kinase BRK)
          Length = 451

 Score = 32.3 bits (72), Expect = 0.71
 Identities = 18/49 (36%), Positives = 23/49 (46%)
 Frame = -3

Query: 422 GSCIQRPCLTDSPTPNLEERFREGPREAETDCRQEGPGGAREVHQGVWR 276
           G  +  PC    P P       E PRE  T CR+ G G   EV +G+W+
Sbjct: 163 GLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGYFGEVFEGLWK 211



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>ZNHI4_MOUSE (Q99PT3) Zinc finger HIT domain-containing protein 4|
           (PAP-1-associated protein 1) (PAPA-1) (High mobility
           group AT-hook 1-like 4)
          Length = 345

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 20/55 (36%), Positives = 23/55 (41%)
 Frame = +1

Query: 217 VVRLCMGRESSRGCVPLSPPRQTPW*TSRAPPGPSCRQSVSASRGPSRNRSSRLG 381
           +VR C G + S    P   P  T      AP GP+ R SV     P R   SR G
Sbjct: 261 MVRYCSGAQGSTLSFPPGVPTPTAVAQRPAPSGPAPRCSVPGCPHPRRYACSRTG 315



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>ECLC_BOVIN (P54281) Epithelial chloride channel protein (Calcium-activated|
           chloride channel)
          Length = 903

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 19/51 (37%), Positives = 23/51 (45%), Gaps = 9/51 (17%)
 Frame = -3

Query: 458 IEATRCCADH---------DAGSCIQRPCLTDSPTPNLEERFREGPREAET 333
           IEATRC               GSCI RPC  DS T   E +    P +++T
Sbjct: 182 IEATRCSTHITGTNVIVKCQGGSCITRPCRRDSQTGLYEAKCTFIPEKSQT 232



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>MASS1_MOUSE (Q8VHN7) Monogenic audiogenic seizure susceptibility protein 1|
            precursor (Very large G-protein coupled receptor 1)
            (Neurepin)
          Length = 6298

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 13/32 (40%), Positives = 22/32 (68%)
 Frame = +3

Query: 219  GAAVHGSREFSRLRTTQPSSPNSLVNFSCTSR 314
            GAA++ S  F+ ++ ++  +P SLV+FS  SR
Sbjct: 5467 GAAINNSARFAPIKLSKSGAPQSLVSFSVGSR 5498



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>DYN1_HUMAN (Q05193) Dynamin-1 (EC 3.6.5.5)|
          Length = 864

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 17/38 (44%), Positives = 18/38 (47%)
 Frame = +1

Query: 262 PLSPPRQTPW*TSRAPPGPSCRQSVSASRGPSRNRSSR 375
           P  PP Q P   +RAPPG   R   S    PSR  S R
Sbjct: 825 PFGPPPQVPSRPNRAPPGVPSR---SGQASPSRPESPR 859



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>BAG3_MOUSE (Q9JLV1) BAG family molecular chaperone regulator 3 (BCL-2-binding|
           athanogene-3) (BAG-3) (Bcl-2-binding protein Bis)
          Length = 577

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 17/39 (43%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
 Frame = +1

Query: 277 RQTPW*TSRAPP-GPSCRQSVSASRGPSRNRSSRLGVGE 390
           R T W   R PP GP  + + S++ GPSRN S  L + E
Sbjct: 47  RTTTWNDPRVPPEGP--KDTASSANGPSRNGSRLLPIRE 83



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>TOM40_MOUSE (Q9QYA2) Probable mitochondrial import receptor subunit TOM40|
           homolog (Translocase of outer membrane 40 kDa subunit
           homolog)
          Length = 359

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 18/52 (34%), Positives = 21/52 (40%), Gaps = 5/52 (9%)
 Frame = +1

Query: 232 MGRESSRGCVPLSPPRQTPW*TSRAPPGPS-----CRQSVSASRGPSRNRSS 372
           MG   +    P  PP  TP      PP PS     CR+S +   G S  R S
Sbjct: 1   MGNVLAASSPPARPPPPTPSLVGLPPPPPSPPRSLCRRSAALGTGSSTGRGS 52



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>VPS4_SCHPO (Q09803) Suppressor protein of bem1/bed5 double mutants|
          Length = 432

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 14/63 (22%), Positives = 31/63 (49%)
 Frame = -1

Query: 490 KTDGDMWMPCGSKQPGAVQTTMQDLASKGLASQILPPPISKSDFEKVLARQRPTVGKKDL 311
           K++  +  PC    P A +++  ++  +     I+ P ++  DF   + + +PT+   D+
Sbjct: 363 KSNRTLVTPCSPGDPDAFESSWLEVNPE----DIMEPKLTVRDFYSAVRKVKPTLNAGDI 418

Query: 310 EVH 302
           E H
Sbjct: 419 EKH 421



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>WIRE_MOUSE (Q6PEV3) WIP-related protein (WASP-interacting protein-related|
           protein)
          Length = 440

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 20/51 (39%), Positives = 21/51 (41%), Gaps = 6/51 (11%)
 Frame = +1

Query: 223 RLCMGRESSRGCVPLSPP------RQTPW*TSRAPPGPSCRQSVSASRGPS 357
           RL  GRE      P+ PP      R  P   S APP P  RQ      GPS
Sbjct: 216 RLHPGREGHPAPPPVKPPPSPVNIRTGPSGQSLAPPPPPYRQPPGVPNGPS 266



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>WIRE_HUMAN (Q8TF74) WIP-related protein (WASP-interacting protein-related|
           protein) (WIP-and CR16-homologous protein)
          Length = 440

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 20/51 (39%), Positives = 21/51 (41%), Gaps = 6/51 (11%)
 Frame = +1

Query: 223 RLCMGRESSRGCVPLSPP------RQTPW*TSRAPPGPSCRQSVSASRGPS 357
           RL  GRE      P+ PP      R  P   S APP P  RQ      GPS
Sbjct: 216 RLHPGREGPPAPPPVKPPPSPVNIRTGPSGQSLAPPPPPYRQPPGVPNGPS 266



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>PHO4_NEUCR (P15710) Phosphate-repressible phosphate permease|
          Length = 590

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 13/25 (52%), Positives = 18/25 (72%)
 Frame = -1

Query: 124 YGDDGSLVMLGYHCASVVNSISLTL 50
           + DD +L+MLG  CA V +SI LT+
Sbjct: 80  FADDPALLMLGMVCAVVASSIYLTM 104



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>CEMA_MARPO (P12211) Chloroplast envelope membrane protein|
          Length = 434

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 22/87 (25%), Positives = 40/87 (45%), Gaps = 12/87 (13%)
 Frame = -3

Query: 257 QPRELSRPMHSRTTEMYIVYNFDSLRLGVK-FADLYTLVALT-----------VLNVWRR 114
           Q ++L++ +H +T E+  +YN DS+++ +    DL   + L+           +LN W  
Sbjct: 285 QLQDLTKEIHQQTIELVQIYNNDSIKIVLHLLTDLIWFITLSCLFILGKERLVILNSW-- 342

Query: 113 RQPCYAGLSLCLCCQLYFTYTLLCLEF 33
            Q  +  LS  +        T LC+ F
Sbjct: 343 AQELFYSLSDTMKAFFILLLTDLCIGF 369


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,590,927
Number of Sequences: 219361
Number of extensions: 1650823
Number of successful extensions: 5375
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 5078
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5369
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3478785780
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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