ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart32h02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RETO_ESCCA (P30986) Reticuline oxidase precursor (EC 1.21.3.3) (... 82 7e-16
2RETO_PAPSO (P93479) Reticuline oxidase precursor (EC 1.21.3.3) (... 76 4e-14
3MCRA_STRLA (P43485) Mitomycin radical oxidase (EC 1.5.3.-) 32 1.1
4RNH2_PSE14 (Q48F73) Ribonuclease HII (EC 3.1.26.4) (RNase HII) 29 7.3

>RETO_ESCCA (P30986) Reticuline oxidase precursor (EC 1.21.3.3)|
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase)
          Length = 538

 Score = 82.0 bits (201), Expect = 7e-16
 Identities = 41/88 (46%), Positives = 52/88 (59%), Gaps = 5/88 (5%)
 Frame = -2

Query: 449 WIREFYAFMAPFVSSSPREAYFNYRDLDLG-----ENVVVGNVSSFQAGMVWGQKYFKGN 285
           W+ + Y FM PFVS +PR  Y N+ DLDLG        VV N  + +    WG+ YF  N
Sbjct: 436 WLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNN--AIEISRSWGESYFLSN 493

Query: 284 YQRLAMAKAQIDPDDYFRNEQSIPPFAN 201
           Y+RL  AK  IDP++ F + QSIPP AN
Sbjct: 494 YERLIRAKTLIDPNNVFNHPQSIPPMAN 521



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>RETO_PAPSO (P93479) Reticuline oxidase precursor (EC 1.21.3.3)|
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase)
          Length = 535

 Score = 76.3 bits (186), Expect = 4e-14
 Identities = 37/86 (43%), Positives = 52/86 (60%), Gaps = 4/86 (4%)
 Frame = -2

Query: 455 TRWIREFYAFMAPFVSSSPREAYFNYRDLDLGENVVVGNVSS----FQAGMVWGQKYFKG 288
           + W+ +FY ++ PFVS  PR  Y N+ DLD+G  +   N SS     +    WG++YF  
Sbjct: 438 SEWLAKFYDYLEPFVSKEPRVGYVNHIDLDIG-GIDWRNKSSTTNAVEIARNWGERYFSS 496

Query: 287 NYQRLAMAKAQIDPDDYFRNEQSIPP 210
           NY+RL  AK  IDP++ F + QSIPP
Sbjct: 497 NYERLVKAKTLIDPNNVFNHPQSIPP 522



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>MCRA_STRLA (P43485) Mitomycin radical oxidase (EC 1.5.3.-)|
          Length = 447

 Score = 31.6 bits (70), Expect = 1.1
 Identities = 14/30 (46%), Positives = 18/30 (60%)
 Frame = -2

Query: 299 YFKGNYQRLAMAKAQIDPDDYFRNEQSIPP 210
           Y   ++ RL   KAQ DPD+ FR   +IPP
Sbjct: 413 YTPADFARLRAVKAQYDPDNMFRVNFNIPP 442



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>RNH2_PSE14 (Q48F73) Ribonuclease HII (EC 3.1.26.4) (RNase HII)|
          Length = 222

 Score = 28.9 bits (63), Expect = 7.3
 Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
 Frame = +3

Query: 186 VRSPAIGKWRDALLVPEVVVGVD-LGLGHRQPLVVTLEVLLAPDHP 320
           +RSP +    D  LV ++V GVD +G G     VVT  V+L P  P
Sbjct: 1   MRSPIMQTGLDFTLVEDLVAGVDEVGRGPLCGAVVTAAVILDPARP 46


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,510,751
Number of Sequences: 219361
Number of extensions: 1009589
Number of successful extensions: 2404
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 2350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2402
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3246866728
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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