ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart32a07
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GI1L3_ARATH (Q940G6) Probable gibberellin receptor GID1L3 (EC 3.... 100 2e-21
2GI1L1_ARATH (Q9MAA7) Probable gibberellin receptor GID1L1 (EC 3.... 96 6e-20
3GI1L2_ARATH (Q9LYC1) Probable gibberellin receptor GID1L2 (EC 3.... 95 1e-19
4GID1_ORYSA (Q6L545) Gibberellin receptor GID1 (EC 3.-.-.-) (Gibb... 82 6e-16
5BAH_STRHY (Q01109) Acetyl-hydrolase (EC 3.1.1.-) 39 0.008
6TRAD1_ECOLI (P09130) Protein traD 32 0.77
7RPOB_STAPU (Q32RW5) DNA-directed RNA polymerase beta chain (EC 2... 31 1.7
8BCHY_RHOCA (P26178) Chlorophyllide reductase 52.5 kDa chain (EC ... 30 2.9
9SHBG_HUMAN (P04278) Sex hormone-binding globulin precursor (SHBG... 30 2.9
10PRP28_YEAST (P23394) Pre-mRNA-splicing ATP-dependent RNA helicas... 30 3.8
11LAMA5_HUMAN (O15230) Laminin alpha-5 chain precursor 30 5.0
12CBLB_XENTR (Q6DFR2) E3 ubiquitin-protein ligase CBL-B (EC 6.3.2.... 29 6.5
13Y1014_TREPA (O83978) Hypothetical protein TP1014 29 8.5
14LRCH4_HUMAN (O75427) Leucine-rich repeats and calponin homology ... 29 8.5
15AVR2A_RAT (P38444) Activin receptor type 2A precursor (EC 2.7.11... 29 8.5
16AVR2A_MOUSE (P27038) Activin receptor type 2A precursor (EC 2.7.... 29 8.5
17LIPS_SPETR (Q9R101) Hormone-sensitive lipase (EC 3.1.1.79) (HSL) 29 8.5

>GI1L3_ARATH (Q940G6) Probable gibberellin receptor GID1L3 (EC 3.-.-.-)|
           (GID1-like protein 3)
          Length = 344

 Score =  100 bits (249), Expect = 2e-21
 Identities = 54/123 (43%), Positives = 71/123 (57%)
 Frame = -1

Query: 509 FGGEERTEAEQRLEGVAPVVNMRRSDWAWRAFLPEGADRNHPAAHVTGEAGPEPELAESF 330
           FGG ERTE+E+RL+G    V +R  DW WRAFLPEG DR HPA    G      E   SF
Sbjct: 219 FGGTERTESEKRLDGKY-FVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLE-GLSF 276

Query: 329 PPAMVAVGGLDPLQDWQRRYGAMLRRKGKAVNVLEFPDAIHAFYCFPELPDSGRLVEEMR 150
           P ++V V GLD +QDWQ +Y   L++ G+ V +L    A   FY  P       +++E+ 
Sbjct: 277 PKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIA 336

Query: 149 AFI 141
           AF+
Sbjct: 337 AFV 339



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>GI1L1_ARATH (Q9MAA7) Probable gibberellin receptor GID1L1 (EC 3.-.-.-)|
           (GID1-like protein 1)
          Length = 345

 Score = 95.9 bits (237), Expect = 6e-20
 Identities = 50/123 (40%), Positives = 70/123 (56%)
 Frame = -1

Query: 509 FGGEERTEAEQRLEGVAPVVNMRRSDWAWRAFLPEGADRNHPAAHVTGEAGPEPELAESF 330
           FGG ERTE+E+ L+G    V +R  DW W+AFLPEG DR HPA +     G   E   SF
Sbjct: 221 FGGNERTESEKSLDGKY-FVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLE-GVSF 278

Query: 329 PPAMVAVGGLDPLQDWQRRYGAMLRRKGKAVNVLEFPDAIHAFYCFPELPDSGRLVEEMR 150
           P ++V V GLD ++DWQ  Y   L++ G+ V ++    A   FY  P       +++E+ 
Sbjct: 279 PKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEIS 338

Query: 149 AFI 141
           AF+
Sbjct: 339 AFV 341



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>GI1L2_ARATH (Q9LYC1) Probable gibberellin receptor GID1L2 (EC 3.-.-.-)|
           (GID1-like protein 2)
          Length = 358

 Score = 95.1 bits (235), Expect = 1e-19
 Identities = 51/123 (41%), Positives = 72/123 (58%)
 Frame = -1

Query: 509 FGGEERTEAEQRLEGVAPVVNMRRSDWAWRAFLPEGADRNHPAAHVTGEAGPEPELAESF 330
           FGG+ERT++E+ L+G    V ++  DW WRA+LPEG DR+HPA +  G  G   +   +F
Sbjct: 221 FGGQERTQSEKTLDGKY-FVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLK-GVNF 278

Query: 329 PPAMVAVGGLDPLQDWQRRYGAMLRRKGKAVNVLEFPDAIHAFYCFPELPDSGRLVEEMR 150
           P ++V V GLD +QDWQ  Y   L++ G  VN+L    A   FY  P       L+EE+ 
Sbjct: 279 PKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELN 338

Query: 149 AFI 141
            F+
Sbjct: 339 KFV 341



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>GID1_ORYSA (Q6L545) Gibberellin receptor GID1 (EC 3.-.-.-)|
           (Gibberellin-insensitive dwarf protein 1) (Protein
           GIBBERELLIN INSENSITIVE DWARF1)
          Length = 354

 Score = 82.4 bits (202), Expect = 6e-16
 Identities = 48/126 (38%), Positives = 67/126 (53%)
 Frame = -1

Query: 509 FGGEERTEAEQRLEGVAPVVNMRRSDWAWRAFLPEGADRNHPAAHVTGEAGPEPELAESF 330
           FGG ERTE+E+RL+G    V ++  DW W+A+LPE ADR+HPA +  G  G        F
Sbjct: 228 FGGTERTESERRLDGKY-FVTLQDRDWYWKAYLPEDADRDHPACNPFGPNGRRLG-GLPF 285

Query: 329 PPAMVAVGGLDPLQDWQRRYGAMLRRKGKAVNVLEFPDAIHAFYCFPELPDSGRLVEEMR 150
             +++ V GLD   D Q  Y   LR  G  V V++  +A   FY  P       ++EE+ 
Sbjct: 286 AKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCENATVGFYLLPNTVHYHEVMEEIS 345

Query: 149 AFIGTN 132
            F+  N
Sbjct: 346 DFLNAN 351



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>BAH_STRHY (Q01109) Acetyl-hydrolase (EC 3.1.1.-)|
          Length = 299

 Score = 38.9 bits (89), Expect = 0.008
 Identities = 26/91 (28%), Positives = 38/91 (41%), Gaps = 1/91 (1%)
 Frame = -1

Query: 404 GADRNHPAAHVTGEAGPEPELAESFPPAMVAVGGLDPLQDWQRRYGAMLRRKGKAVNVLE 225
           G D  HP A       P        PP ++ VG  + L D  R       + G  V   E
Sbjct: 208 GTDPRHPLA------SPAHGDLTGLPPLLIQVGSEEVLHDDARALEQAALKAGTPVTFEE 261

Query: 224 FPDAIHAFYCF-PELPDSGRLVEEMRAFIGT 135
           +P+  H ++ + P LP+  R +E   AF+ T
Sbjct: 262 WPEMFHVWHWYHPVLPEGRRAIEVAGAFLRT 292



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>TRAD1_ECOLI (P09130) Protein traD|
          Length = 717

 Score = 32.3 bits (72), Expect = 0.77
 Identities = 28/101 (27%), Positives = 45/101 (44%), Gaps = 10/101 (9%)
 Frame = -1

Query: 503 GEERTEAEQRLEGVAPVVNMRRSDWAWRAFLPEGADRNHPAAHVTGE--AGPEPELAESF 330
           GE+ T+AEQ  + V+P +N ++SD          +  N PA  +  E    PE E+ +  
Sbjct: 613 GEDVTQAEQPQQPVSPAINDKKSD----------SGVNVPAGGIEQELKMKPEEEMEQQL 662

Query: 329 PPAMVAVG---GLDPLQDWQRR-----YGAMLRRKGKAVNV 231
           PP +   G    +   + WQ+         M RR+   +NV
Sbjct: 663 PPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINV 703



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>RPOB_STAPU (Q32RW5) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase beta subunit) (RNA
           polymerase beta subunit)
          Length = 1068

 Score = 31.2 bits (69), Expect = 1.7
 Identities = 16/44 (36%), Positives = 25/44 (56%)
 Frame = +3

Query: 117 VMSGRVGPDERPHLLHEPAGVGELREAVEGVDGVRELQHIDRLP 248
           V+ G++ P E PHLL  P   G+L +A+ GV  +   +   +LP
Sbjct: 737 VLVGKLAPLEAPHLLRSPE--GKLLQAIFGVQAITTRESCLKLP 778



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>BCHY_RHOCA (P26178) Chlorophyllide reductase 52.5 kDa chain (EC 1.18.1.-)|
           (Chlorin reductase)
          Length = 497

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 30/106 (28%), Positives = 41/106 (38%), Gaps = 2/106 (1%)
 Frame = -1

Query: 509 FGGEERTEAEQRLEGVAPVVNMRRSDWAWRAFLPEGADRNHPAAHVTGEAGPEPELA--E 336
           FG     EA+  L G A +   R+   A     P     + P   + GE  P   +    
Sbjct: 162 FGVPTHAEAKDVLSG-AMLAYARQEVMAGPVPAPISGRSDRPTVTLLGEMFPADPMVIGA 220

Query: 335 SFPPAMVAVGGLDPLQDWQRRYGAMLRRKGKAVNVLEFPDAIHAFY 198
              P  +AVG   P++DW+  Y A+     K V       AIH FY
Sbjct: 221 MLAPMGLAVGPTVPMRDWRELYAAL---DSKVV------AAIHPFY 257



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>SHBG_HUMAN (P04278) Sex hormone-binding globulin precursor (SHBG) (Sex|
           steroid-binding protein) (SBP) (Testis-specific
           androgen-binding protein) (ABP)
           (Testosterone-estrogen-binding globulin)
           (Testosterone-estradiol-binding globulin) (TeBG)
          Length = 402

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 18/71 (25%), Positives = 26/71 (36%), Gaps = 18/71 (25%)
 Frame = -1

Query: 440 RSDWAWRAFLPEGADRNHPAAHVTGEAGPEPELAESF------------------PPAMV 315
           R  WA R  LP  +  + PA H++   G EP    +F                  P  ++
Sbjct: 25  RQGWALRPVLPTQSAHDPPAVHLSNGPGQEPIAVMTFDLTKITKTSSSFEVRTWDPEGVI 84

Query: 314 AVGGLDPLQDW 282
             G  +P  DW
Sbjct: 85  FYGDTNPKDDW 95



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>PRP28_YEAST (P23394) Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 (EC|
           3.6.1.-) (Helicase CA8)
          Length = 588

 Score = 30.0 bits (66), Expect = 3.8
 Identities = 23/79 (29%), Positives = 39/79 (49%), Gaps = 9/79 (11%)
 Frame = -1

Query: 488 EAEQRLEGVAPVVNMRRSDWAWRAFLPE-GADRNHPAAHVTGEAGPEPE------LAESF 330
           E+E  L G  P+V+ R  D  W+   P+  A+ ++   H T ++  E        L E +
Sbjct: 99  ESEDTLSGYDPIVSTRAIDLLWKGKTPKNAAESSYMGKHWTEKSLHEMNERDWRILKEDY 158

Query: 329 PPAMVAVGGL--DPLQDWQ 279
             A+V  GG   +PL++W+
Sbjct: 159 --AIVTKGGTVENPLRNWE 175



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>LAMA5_HUMAN (O15230) Laminin alpha-5 chain precursor|
          Length = 3695

 Score = 29.6 bits (65), Expect = 5.0
 Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
 Frame = +3

Query: 81   LARITQIFSSIGVMSGRVGPDERPHLLHEPAGVGELREAV-EGVDGVRELQHID 239
            LAR+ +  SS+   +  +    R  L    AG+ +LREA+   VD  RE Q ++
Sbjct: 2349 LARVQEQLSSLWEENQALATQTRDRLAQHEAGLMDLREALNRAVDATREAQELN 2402



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>CBLB_XENTR (Q6DFR2) E3 ubiquitin-protein ligase CBL-B (EC 6.3.2.-) (Signal|
           transduction protein CBL-B) (SH3-binding protein CBL-B)
          Length = 982

 Score = 29.3 bits (64), Expect = 6.5
 Identities = 12/19 (63%), Positives = 14/19 (73%), Gaps = 3/19 (15%)
 Frame = +3

Query: 417 RPPRPVG---APHVHHRRH 464
           RPP+PV    AP +HHRRH
Sbjct: 904 RPPKPVPRRTAPEIHHRRH 922



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>Y1014_TREPA (O83978) Hypothetical protein TP1014|
          Length = 644

 Score = 28.9 bits (63), Expect = 8.5
 Identities = 13/35 (37%), Positives = 20/35 (57%)
 Frame = -1

Query: 341 AESFPPAMVAVGGLDPLQDWQRRYGAMLRRKGKAV 237
           AE  P A  A G     ++W +R G +L+++GK V
Sbjct: 598 AEEHPRARPAAGKAQRWREWYQRAGQLLQQQGKTV 632



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>LRCH4_HUMAN (O75427) Leucine-rich repeats and calponin homology|
           domain-containing protein 4 (Leucine-rich neuronal
           protein)
          Length = 683

 Score = 28.9 bits (63), Expect = 8.5
 Identities = 27/109 (24%), Positives = 44/109 (40%), Gaps = 1/109 (0%)
 Frame = -1

Query: 410 PEGADRNHPAAHVTGEAGPEPELAESFPPAMVAVGGLD-PLQDWQRRYGAMLRRKGKAVN 234
           P+ +     AA   G   P P   E  P A  A      PL   QR    + R   ++ +
Sbjct: 458 PKSSASQAGAAAGQGAPAPAPASQEPLPIAGPATAPAPRPLGSIQRPNSFLFRSSSQSGS 517

Query: 233 VLEFPDAIHAFYCFPELPDSGRLVEEMRAFIGTNAPTHHPDA*EDLRDA 87
               PD++     +P++PD   L+ ++R  + +      P   EDL +A
Sbjct: 518 GPSSPDSVLRPRRYPQVPDEKDLMTQLRQVLESRLQRPLP---EDLAEA 563



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>AVR2A_RAT (P38444) Activin receptor type 2A precursor (EC 2.7.11.30) (Activin|
           receptor type IIA) (ACTR-IIA)
          Length = 513

 Score = 28.9 bits (63), Expect = 8.5
 Identities = 11/27 (40%), Positives = 14/27 (51%)
 Frame = -2

Query: 307 AGWTRCRTGSGGTAPCCGGRGRRSMCW 227
           A W R RT   G  PC G + +R  C+
Sbjct: 35  ANWERDRTNQTGVEPCYGDKDKRRHCF 61



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>AVR2A_MOUSE (P27038) Activin receptor type 2A precursor (EC 2.7.11.30) (Activin|
           receptor type IIA) (ACTR-IIA)
          Length = 513

 Score = 28.9 bits (63), Expect = 8.5
 Identities = 11/27 (40%), Positives = 14/27 (51%)
 Frame = -2

Query: 307 AGWTRCRTGSGGTAPCCGGRGRRSMCW 227
           A W R RT   G  PC G + +R  C+
Sbjct: 35  ANWERDRTNQTGVEPCYGDKDKRRHCF 61



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>LIPS_SPETR (Q9R101) Hormone-sensitive lipase (EC 3.1.1.79) (HSL)|
          Length = 763

 Score = 28.9 bits (63), Expect = 8.5
 Identities = 16/64 (25%), Positives = 25/64 (39%)
 Frame = -1

Query: 344 LAESFPPAMVAVGGLDPLQDWQRRYGAMLRRKGKAVNVLEFPDAIHAFYCFPELPDSGRL 165
           + ++ PP  +    LDP+ D    +   LR  G+ V +    D  H F     L    R 
Sbjct: 677 MLKTLPPVHIVACALDPMLDDSXMFARRLRALGQPVTLRVVEDLPHGFLSLASLCRETRQ 736

Query: 164 VEEM 153
             E+
Sbjct: 737 AAEL 740


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,987,094
Number of Sequences: 219361
Number of extensions: 1019557
Number of successful extensions: 3704
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 3539
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3694
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3754426130
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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