ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart30h10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NFRKB_XENTR (Q6P4L9) Nuclear factor related to kappa-B-binding p... 33 0.46
2GUX2_TRIRE (P07987) Exoglucanase 2 precursor (EC 3.2.1.91) (Exog... 32 0.61
3FADA_ERWCT (Q6DAP6) 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (Fatty... 32 1.0
4RL172_HORVU (P35267) 60S ribosomal protein L17-2 31 1.3
5TERT_CANFA (Q6A548) Telomerase reverse transcriptase (EC 2.7.7.4... 31 1.8
6ADA1B_RAT (P15823) Alpha-1B adrenergic receptor (Alpha 1B-adreno... 30 2.3
7AP180_MOUSE (Q61548) Clathrin coat assembly protein AP180 (Clath... 30 2.3
8HE_PARLI (P22757) Hatching enzyme precursor (EC 3.4.24.12) (HE) ... 30 3.0
9RFAY_XANCP (P46358) Probable RNA polymerase sigma factor rfaY 30 3.9
10TENS_CHICK (Q04205) Tensin 29 5.1
11AP180_RAT (Q05140) Clathrin coat assembly protein AP180 (Clathri... 29 5.1
12MMP15_MOUSE (O54732) Matrix metalloproteinase-15 precursor (EC 3... 29 5.1
13CSD1_MYCLE (O32975) Probable cysteine desulfurase 1 (EC 2.8.1.7) 29 5.1
14SPEE_PROMM (Q7V3X3) Spermidine synthase (EC 2.5.1.16) (Putrescin... 29 5.1
15ADA1B_MOUSE (P97717) Alpha-1B adrenergic receptor (Alpha 1B-adre... 29 6.7
16CO1A2_BOVIN (P02465) Collagen alpha-2(I) chain precursor 28 8.7
17LACD2_ENTFA (Q833W8) Tagatose 1,6-diphosphate aldolase 2 (EC 4.1... 28 8.7
18DBP8_DEBHA (Q6BGU8) ATP-dependent RNA helicase DBP8 (EC 3.6.1.-) 28 8.7
19UL50_HCMVA (P16791) Protein UL50 (HFLF4 protein) 28 8.7

>NFRKB_XENTR (Q6P4L9) Nuclear factor related to kappa-B-binding protein|
           (DNA-binding protein R kappa-B)
          Length = 1265

 Score = 32.7 bits (73), Expect = 0.46
 Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
 Frame = +1

Query: 292 SCTNTSVAPSPGRRARATMPAV*MAPILPGASG-VHPVGASTMTRSHSTS 438
           S T +S+ P+PG     T PA+   P+ P  S  V+ + AST+T +  +S
Sbjct: 707 SLTESSMPPTPGTPGTPTPPALPSTPMSPSTSATVNKIVASTITETQKSS 756



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>GUX2_TRIRE (P07987) Exoglucanase 2 precursor (EC 3.2.1.91) (Exoglucanase II)|
           (Exocellobiohydrolase II) (CBHII)
           (1,4-beta-cellobiohydrolase)
          Length = 471

 Score = 32.3 bits (72), Expect = 0.61
 Identities = 34/110 (30%), Positives = 47/110 (42%), Gaps = 4/110 (3%)
 Frame = +1

Query: 109 LMNVSATVDDEKNY*LRGQKGVPGFSLWLGITKCLIRPTC--SARYASQRNALENLSSTV 282
           L  ++A+V  E+        G  G   W G T C    TC  S  Y SQ       SS+ 
Sbjct: 12  LATLAASVPLEERQACSSVWGQCGGQNWSGPTCCASGSTCVYSNDYYSQCLPGAASSSSS 71

Query: 283 RSTSCTNTSVAPSPGRRARATMPAV*MAPILPGA--SGVHPVGASTMTRS 426
              + T + V+P+  R + AT P        PG+  + V PVG+ T T S
Sbjct: 72  TRAASTTSRVSPTTSRSSSATPP--------PGSTTTRVPPVGSGTATYS 113



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>FADA_ERWCT (Q6DAP6) 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (Fatty oxidation|
           complex beta subunit) (Beta-ketothiolase) (Acetyl-CoA
           acyltransferase)
          Length = 387

 Score = 31.6 bits (70), Expect = 1.0
 Identities = 21/65 (32%), Positives = 33/65 (50%)
 Frame = -1

Query: 386 EAPGRMGAIYTAGMVARARRPGDGATDVFVHDVDRTVEDRFSKAFLCDAYLAEQVGRIRH 207
           +A G MG   TA M+AR            +H++ R ++D+F+      AY A Q G  RH
Sbjct: 142 KAAGMMGL--TAEMLAR------------MHNISREMQDQFAARSHQRAYHATQSGAFRH 187

Query: 206 FVIPS 192
            ++P+
Sbjct: 188 EIVPT 192



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>RL172_HORVU (P35267) 60S ribosomal protein L17-2|
          Length = 172

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 18/61 (29%), Positives = 29/61 (47%)
 Frame = -3

Query: 261 QGVPLRRVPRRAGRPDQALRNPQPQGEARHALLPSQLVIFFIIHCSTYVHQAYLKTQHIY 82
           Q +P RR  R  GR  Q ++N QP G+ R     +Q V+  + +  +      L   ++Y
Sbjct: 56  QAIPFRRYCRGVGRTAQ-VKNRQPNGQGRWPAKSAQFVLDLLKNAESNAEVKGLDVDNLY 114

Query: 81  I 79
           I
Sbjct: 115 I 115



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>TERT_CANFA (Q6A548) Telomerase reverse transcriptase (EC 2.7.7.49) (Telomerase|
           catalytic subunit)
          Length = 1123

 Score = 30.8 bits (68), Expect = 1.8
 Identities = 32/106 (30%), Positives = 41/106 (38%), Gaps = 9/106 (8%)
 Frame = +1

Query: 154 LRGQKGVPGFSLWLGITKCLIRPTCSARYASQRNALENLSSTVRSTSCTNTSVAPSPGRR 333
           L G  G+PG     G +  L RPT  A+ +  R    +  S V        SVA  P R 
Sbjct: 191 LPGLPGLPGLGAGAGASADL-RPTRQAQNSGARRRRGSPGSGVPLAKRPRRSVASEPERG 249

Query: 334 A-----RATMPAV*MAPILPGASGVHPV----GASTMTRSHSTSWN 444
           A     RA  P V  AP +  A    P     G    TR  + +W+
Sbjct: 250 AHRSFPRAQQPPVSEAPAVTPAVAASPAASWEGGPPGTRPTTPAWH 295



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>ADA1B_RAT (P15823) Alpha-1B adrenergic receptor (Alpha 1B-adrenoceptor)|
           (Alpha 1B-adrenoreceptor)
          Length = 515

 Score = 30.4 bits (67), Expect = 2.3
 Identities = 15/41 (36%), Positives = 23/41 (56%)
 Frame = +1

Query: 235 RYASQRNALENLSSTVRSTSCTNTSVAPSPGRRARATMPAV 357
           R  S++++L++  S +  T  T  S +PSPG   R T P V
Sbjct: 397 RSQSRKDSLDDSGSCMSGTQRTLPSASPSPGYLGRGTQPPV 437



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>AP180_MOUSE (Q61548) Clathrin coat assembly protein AP180 (Clathrin|
           coat-associated protein AP180) (91 kDa
           synaptosomal-associated protein) (Phosphoprotein F1-20)
          Length = 901

 Score = 30.4 bits (67), Expect = 2.3
 Identities = 16/42 (38%), Positives = 17/42 (40%), Gaps = 1/42 (2%)
 Frame = -2

Query: 370 WAPSTPPAWWRARGGPE-TAPPTCSCTTWTGRWRTGSPRRSF 248
           W P   PA W A   P+ T PPT S     G    G P   F
Sbjct: 793 WQPKVTPATWSAGVPPQGTVPPTSSVPPGAGAPSVGQPGAGF 834



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>HE_PARLI (P22757) Hatching enzyme precursor (EC 3.4.24.12) (HE) (HEZ)|
           (Envelysin) (Sea-urchin-hatching proteinase) [Contains:
           Hatching enzyme 18 kDa form]
          Length = 587

 Score = 30.0 bits (66), Expect = 3.0
 Identities = 21/62 (33%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
 Frame = +1

Query: 154 LRGQKGVPGFSLWLGITKCLIRPTCSARYASQRNALENLSSTVRSTSCTNTSVA-PSPGR 330
           + G + + G +   G T    RPT + R  + R      ++T R+T+ T TS + PSP R
Sbjct: 319 IAGIRSLYGSNSGSGTTTTTRRPT-TTRATTTRRTTTTRATTTRATTTTTTSPSRPSPPR 377

Query: 331 RA 336
           RA
Sbjct: 378 RA 379



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>RFAY_XANCP (P46358) Probable RNA polymerase sigma factor rfaY|
          Length = 400

 Score = 29.6 bits (65), Expect = 3.9
 Identities = 31/106 (29%), Positives = 42/106 (39%), Gaps = 4/106 (3%)
 Frame = +1

Query: 148 Y*LRGQKGVPGFSLWLGITKCLIRPTCSARYASQRNALENLSSTVRSTSCTNTSVAPSPG 327
           Y   GQ+     SL LG++   +R   S   A+ RN L     T    S    + A +  
Sbjct: 148 YYREGQRSQQVASL-LGLSDAAVRKRLSRARATVRNELLQRFDTFARGSAPGVAFATTVT 206

Query: 328 RRARATMPAV*MAPILPGA----SGVHPVGASTMTRSHSTSWNAAG 453
                  P    A I  G      GV  +GAS ++ S  TS +AAG
Sbjct: 207 AATMLAAPGTASAAIALGGIGSLGGVGKLGASGLSGSALTSGSAAG 252



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>TENS_CHICK (Q04205) Tensin|
          Length = 1744

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 22/50 (44%), Positives = 24/50 (48%)
 Frame = +1

Query: 253  NALENLSSTVRSTSCTNTSVAPSPGRRARATMPAV*MAPILPGASGVHPV 402
            +AL     T+  T  TNT   PSPG   RA  PAV   P  PG  G H V
Sbjct: 1260 HALPGSPRTLHRTVATNTP--PSPGFGRRAANPAVASVPGSPGL-GRHTV 1306



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>AP180_RAT (Q05140) Clathrin coat assembly protein AP180 (Clathrin|
           coat-associated protein AP180) (91 kDa
           synaptosomal-associated protein)
          Length = 915

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 15/38 (39%), Positives = 16/38 (42%), Gaps = 1/38 (2%)
 Frame = -2

Query: 370 WAPSTPPAWWRARGGPE-TAPPTCSCTTWTGRWRTGSP 260
           W P   PA W A   P+ T PPT S     G    G P
Sbjct: 807 WQPKVTPATWSAGVPPQGTVPPTSSVPPGAGAPSVGQP 844



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>MMP15_MOUSE (O54732) Matrix metalloproteinase-15 precursor (EC 3.4.24.-)|
           (MMP-15) (Membrane-type matrix metalloproteinase 2)
           (MT-MMP 2) (MTMMP2) (Membrane-type-2 matrix
           metalloproteinase) (MT2-MMP) (MT2MMP)
          Length = 657

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 18/33 (54%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
 Frame = -3

Query: 285 PDGGGQVLQGVPLRRVPRRAGRPD-QALRNPQP 190
           PDG  Q  + +P  R PRR GRPD Q  R PQP
Sbjct: 302 PDGKPQPTRPLPTVR-PRRPGRPDHQPPRPPQP 333



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>CSD1_MYCLE (O32975) Probable cysteine desulfurase 1 (EC 2.8.1.7)|
          Length = 611

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 26/96 (27%), Positives = 35/96 (36%), Gaps = 1/96 (1%)
 Frame = -1

Query: 452 PAAFHEVEWDLVMVDAPTGWTPEAPGRMGAIYTAGMVARARR-PGDGATDVFVHDVDRTV 276
           PA   ++    V V APT   PE   ++  +++ G V      P    T V V       
Sbjct: 51  PAPVAQIGVADVYVPAPTSPEPEGLPQVAPVFSRGNVPDTTAVPAAAGTTVGV------- 103

Query: 275 EDRFSKAFLCDAYLAEQVGRIRHFVIPSHREKPGTP 168
                     D YL   VG +  F +PSH   P  P
Sbjct: 104 ---------ADPYLPPGVGHLSGFAVPSHGIVPTVP 130



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>SPEE_PROMM (Q7V3X3) Spermidine synthase (EC 2.5.1.16) (Putrescine|
           aminopropyltransferase) (SPDSY)
          Length = 286

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 14/48 (29%), Positives = 18/48 (37%), Gaps = 12/48 (25%)
 Frame = -2

Query: 370 WAPSTPPAWW----RARGGPETAPP--------TCSCTTWTGRWRTGS 263
           W P  P  WW     A  GP    P        +  C  W+ RW+ G+
Sbjct: 226 WVPMYPSGWWSWTFAAIDGPRYRNPLPARAAAISAGCEIWSPRWQQGA 273



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>ADA1B_MOUSE (P97717) Alpha-1B adrenergic receptor (Alpha 1B-adrenoceptor)|
           (Alpha 1B-adrenoreceptor)
          Length = 514

 Score = 28.9 bits (63), Expect = 6.7
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = +1

Query: 235 RYASQRNALENLSSTVRSTSCTNTSVAPSPGRRARATMPAV 357
           R  S++++L++  S +  +  T  S +PSPG   R T P V
Sbjct: 396 RSQSRKDSLDDSGSCMSGSQRTLPSASPSPGYLGRGTQPPV 436



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>CO1A2_BOVIN (P02465) Collagen alpha-2(I) chain precursor|
          Length = 1364

 Score = 28.5 bits (62), Expect = 8.7
 Identities = 16/34 (47%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = +1

Query: 322 PGRRARATMPAV*MAPILPGASGV-HPVGASTMT 420
           PG +  A +P V  AP LPG  G+  PVGA+  T
Sbjct: 304 PGAKGAAGLPGVAGAPGLPGPRGIPGPVGAAGAT 337



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>LACD2_ENTFA (Q833W8) Tagatose 1,6-diphosphate aldolase 2 (EC 4.1.2.40)|
           (Tagatose-bisphosphate aldolase 2)
           (D-tagatose-1,6-bisphosphate aldolase 2)
          Length = 331

 Score = 28.5 bits (62), Expect = 8.7
 Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 2/69 (2%)
 Frame = -1

Query: 458 GLPAA-FHEVEWDLVMVDAPTGWTPEAPGRMGAIYTAGMVARARRPG-DGATDVFVHDVD 285
           GLPAA   + E  L++    TG+    PGR+  +     V R +  G D    +  +DVD
Sbjct: 74  GLPAAKARDTEAGLLLAYEKTGYDATTPGRLPDLLADWSVLRLKEEGADAIKFLLYYDVD 133

Query: 284 RTVEDRFSK 258
              E    K
Sbjct: 134 EDPEINHQK 142



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>DBP8_DEBHA (Q6BGU8) ATP-dependent RNA helicase DBP8 (EC 3.6.1.-)|
          Length = 441

 Score = 28.5 bits (62), Expect = 8.7
 Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
 Frame = -1

Query: 362 IYTAGMVARARRPGDGATDVFVHDVDR--TVEDRFSK 258
           I+  G  ARA R GD  + V   DVDR   +E+R +K
Sbjct: 338 IHRVGRTARAGRKGDAISIVAEKDVDRILAIEERINK 374



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>UL50_HCMVA (P16791) Protein UL50 (HFLF4 protein)|
          Length = 397

 Score = 28.5 bits (62), Expect = 8.7
 Identities = 11/16 (68%), Positives = 12/16 (75%)
 Frame = -2

Query: 370 WAPSTPPAWWRARGGP 323
           WAPS+   WWRAR GP
Sbjct: 338 WAPSS---WWRARSGP 350


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,529,414
Number of Sequences: 219361
Number of extensions: 1319566
Number of successful extensions: 4021
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 3872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4017
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2968155324
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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