ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart30c03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1OPR1_LYCES (Q9XG54) 12-oxophytodienoate reductase 1 (EC 1.3.1.42... 137 1e-32
2OPR1_ARATH (Q8LAH7) 12-oxophytodienoate reductase 1 (EC 1.3.1.42... 129 5e-30
3OPRL_LYCES (Q9FEX0) 12-oxophytodienoate reductase-like protein (... 129 5e-30
4OPR2_ARATH (Q8GYB8) 12-oxophytodienoate reductase 2 (EC 1.3.1.42... 127 2e-29
5OPR3_LYCES (Q9FEW9) 12-oxophytodienoate reductase 3 (EC 1.3.1.42... 109 4e-24
6OPR3_ARATH (Q9FUP0) 12-oxophytodienoate reductase 3 (EC 1.3.1.42... 102 6e-22
7OPRL1_ARATH (Q8GYA3) Putative 12-oxophytodienoate reductase-like... 97 2e-20
8OYEB_SCHPO (Q09671) Putative NADPH dehydrogenase C5H10.10 (EC 1.... 71 1e-12
9OYEA_SCHPO (Q09670) Putative NADPH dehydrogenase C5H10.04 (EC 1.... 67 3e-11
10OYE3_YEAST (P41816) NADPH dehydrogenase 3 (EC 1.6.99.1) (Old yel... 61 1e-09
11OYE2_YEAST (Q03558) NADPH dehydrogenase 2 (EC 1.6.99.1) (Old yel... 54 2e-07
12OYE1_SACPS (Q02899) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yel... 54 2e-07
13KYE1_KLULA (P40952) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yel... 52 6e-07
14NEMA_ECOLI (P77258) N-ethylmaleimide reductase (EC 1.-.-.-) (N-e... 50 2e-06
15EBP1_CANAL (P43084) Probable NADPH dehydrogenase (EC 1.6.99.1) (... 42 6e-04
16NADO_THEBR (P32382) NADH oxidase (EC 1.-.-.-) 32 0.80
17Y31K_SSV1 (P20203) Hypothetical 31.5 kDa protein (ORF B-277) 30 4.0
18ALX_ECOLI (P42601) Protein alx 29 6.8
19ALX_ECOL6 (Q8FDE1) Protein alx 29 6.8
20ALX_ECO57 (Q8XAJ0) Protein alx 29 6.8
21ALX_SHIFL (Q83Q35) Protein alx 29 6.8
22GLYA_PSYAR (Q4FUZ8) Serine hydroxymethyltransferase (EC 2.1.2.1)... 28 8.9

>OPR1_LYCES (Q9XG54) 12-oxophytodienoate reductase 1 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 1) (OPDA-reductase
           1) (LeOPR1)
          Length = 376

 Score =  137 bits (345), Expect = 1e-32
 Identities = 61/96 (63%), Positives = 76/96 (79%)
 Frame = -1

Query: 467 PRMAIVDGRTQIPHGLLPFRKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANP 288
           PRM     + +    L+P RKA+ GTFI AGGYDRE+GN+ + +D ADLVAYGR+F++NP
Sbjct: 278 PRMKTAWEKIECTESLVPMRKAYKGTFIVAGGYDREDGNRALIEDRADLVAYGRLFISNP 337

Query: 287 DFPKRFELNSALNKYDRNTFYTQDPIVGYTNYPFFE 180
           D PKRFELN+ LNKY+R+TFYT DPIVGYT+YPF E
Sbjct: 338 DLPKRFELNAPLNKYNRDTFYTSDPIVGYTDYPFLE 373



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>OPR1_ARATH (Q8LAH7) 12-oxophytodienoate reductase 1 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 1) (OPDA-reductase
           1) (AtOPR1) (FS-AT-I)
          Length = 372

 Score =  129 bits (323), Expect = 5e-30
 Identities = 57/86 (66%), Positives = 70/86 (81%)
 Frame = -1

Query: 431 PHGLLPFRKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANPDFPKRFELNSAL 252
           PH L+P RKAF GTFI+AGG+ RE+GN+ V+    DLVAYGR FLANPD PKRF++++ L
Sbjct: 286 PHTLMPMRKAFKGTFISAGGFTREDGNEAVSKGRTDLVAYGRWFLANPDLPKRFQVDAPL 345

Query: 251 NKYDRNTFYTQDPIVGYTNYPFFEGS 174
           NKYDR TFYT DP+VGYT+YPF E +
Sbjct: 346 NKYDRPTFYTSDPVVGYTDYPFLEST 371



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>OPRL_LYCES (Q9FEX0) 12-oxophytodienoate reductase-like protein (EC 1.3.1.-)|
           (LeOPR2)
          Length = 355

 Score =  129 bits (323), Expect = 5e-30
 Identities = 54/92 (58%), Positives = 74/92 (80%)
 Frame = -1

Query: 455 IVDGRTQIPHGLLPFRKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANPDFPK 276
           +++ R  +   LLP RKAF GT IA+GGY + +G K + ++YADL+++GR+FLANPD PK
Sbjct: 261 VMEPRETVNRSLLPIRKAFKGTLIASGGYGKSDGEKAIDENYADLISFGRMFLANPDLPK 320

Query: 275 RFELNSALNKYDRNTFYTQDPIVGYTNYPFFE 180
           RFE+N+ LNKY+R+TFYT DPI+GYT+YPF E
Sbjct: 321 RFEVNAPLNKYNRSTFYTNDPIIGYTDYPFLE 352



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>OPR2_ARATH (Q8GYB8) 12-oxophytodienoate reductase 2 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 2) (OPDA-reductase
           2) (AtOPR2)
          Length = 374

 Score =  127 bits (318), Expect = 2e-29
 Identities = 59/98 (60%), Positives = 73/98 (74%)
 Frame = -1

Query: 467 PRMAIVDGRTQIPHGLLPFRKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANP 288
           PRM  V       H L+P R+AF GTFI+AGG+ RE+GN+ VA    DLVAYGR FLANP
Sbjct: 276 PRMKTVGEIAACSHTLMPMREAFKGTFISAGGFTREDGNEAVAKGRTDLVAYGRWFLANP 335

Query: 287 DFPKRFELNSALNKYDRNTFYTQDPIVGYTNYPFFEGS 174
           D PKRF+L++ LNKY+R+TFYT DP+VGYT+YP  E +
Sbjct: 336 DLPKRFQLDAPLNKYNRSTFYTSDPVVGYTDYPSLEST 373



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>OPR3_LYCES (Q9FEW9) 12-oxophytodienoate reductase 3 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 3) (OPDA-reductase
           3) (LeOPR3)
          Length = 396

 Score =  109 bits (272), Expect = 4e-24
 Identities = 48/81 (59%), Positives = 63/81 (77%)
 Frame = -1

Query: 410 RKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANPDFPKRFELNSALNKYDRNT 231
           R A+ GTFI +GGY RE G + VA   ADLV+YGR+F++NPD   R +LN+ LNKY+R T
Sbjct: 309 RNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNKYNRKT 368

Query: 230 FYTQDPIVGYTNYPFFEGSNA 168
           FYTQDP+VGYT+YPF +G+ +
Sbjct: 369 FYTQDPVVGYTDYPFLQGNGS 389



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>OPR3_ARATH (Q9FUP0) 12-oxophytodienoate reductase 3 (EC 1.3.1.42)|
           (12-oxophytodienoate-10,11-reductase 3) (OPDA-reductase
           3) (Delayed dehiscence 1) (AtOPR3)
          Length = 391

 Score =  102 bits (253), Expect = 6e-22
 Identities = 43/75 (57%), Positives = 61/75 (81%)
 Frame = -1

Query: 410 RKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANPDFPKRFELNSALNKYDRNT 231
           R A++GTF+++GG+++E G + V    ADLV+YGR+F+ANPD   RF+++  LNKY+R T
Sbjct: 310 RMAYNGTFMSSGGFNKELGMQAVQQGDADLVSYGRLFIANPDLVSRFKIDGELNKYNRKT 369

Query: 230 FYTQDPIVGYTNYPF 186
           FYTQDP+VGYT+YPF
Sbjct: 370 FYTQDPVVGYTDYPF 384



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>OPRL1_ARATH (Q8GYA3) Putative 12-oxophytodienoate reductase-like protein 1 (EC|
           1.3.1.-)
          Length = 324

 Score = 97.1 bits (240), Expect = 2e-20
 Identities = 47/74 (63%), Positives = 53/74 (71%)
 Frame = -1

Query: 467 PRMAIVDGRTQIPHGLLPFRKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANP 288
           PRM  V    +    L P R AF+GTFI AGGY RE+GNK VA+   DLVAYGR+FLANP
Sbjct: 251 PRMKTVSEIFECRESLTPMRNAFNGTFIVAGGYTREDGNKAVAEGRTDLVAYGRLFLANP 310

Query: 287 DFPKRFELNSALNK 246
           D PKRFELN+ LNK
Sbjct: 311 DLPKRFELNAPLNK 324



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>OYEB_SCHPO (Q09671) Putative NADPH dehydrogenase C5H10.10 (EC 1.6.99.1) (Old|
           yellow enzyme homolog)
          Length = 392

 Score = 71.2 bits (173), Expect = 1e-12
 Identities = 36/78 (46%), Positives = 50/78 (64%)
 Frame = -1

Query: 419 LPFRKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANPDFPKRFELNSALNKYD 240
           L ++  +   FI AGG+DR+   ++ A+    LVA+GR FL+NPD P R + N  LNK+D
Sbjct: 305 LIYKNLWGDPFITAGGHDRDSAIQM-AEQENTLVAFGRYFLSNPDLPFRLKYNLPLNKWD 363

Query: 239 RNTFYTQDPIVGYTNYPF 186
           R TFYT+    GY +YPF
Sbjct: 364 RATFYTKMSPKGYIDYPF 381



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>OYEA_SCHPO (Q09670) Putative NADPH dehydrogenase C5H10.04 (EC 1.6.99.1) (Old|
           yellow enzyme homolog)
          Length = 382

 Score = 66.6 bits (161), Expect = 3e-11
 Identities = 35/75 (46%), Positives = 48/75 (64%)
 Frame = -1

Query: 410 RKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANPDFPKRFELNSALNKYDRNT 231
           +K + G FI AGGYD E   +  A++   LVA+GR F+ANPD   R + +  LNK+DR++
Sbjct: 301 QKYWKGPFITAGGYDPETAVQA-ANERGVLVAFGRNFIANPDLVFRIKHHIPLNKWDRSS 359

Query: 230 FYTQDPIVGYTNYPF 186
           FY      GYT+YPF
Sbjct: 360 FYLPKTEKGYTDYPF 374



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>OYE3_YEAST (P41816) NADPH dehydrogenase 3 (EC 1.6.99.1) (Old yellow enzyme 3)|
          Length = 399

 Score = 61.2 bits (147), Expect = 1e-09
 Identities = 38/97 (39%), Positives = 52/97 (53%), Gaps = 4/97 (4%)
 Frame = -1

Query: 458 AIVDGRTQIPHGLLPFRKA-FDGTFIAAGGYDREEGNKVVADDYAD---LVAYGRIFLAN 291
           ++V+G  +   G   F  + + G  I AG Y      +VV +   D   L+ YGR F++N
Sbjct: 296 SLVEGEGEYSEGTNDFAYSIWKGPIIRAGNYALHP--EVVREQVKDPRTLIGYGRFFISN 353

Query: 290 PDFPKRFELNSALNKYDRNTFYTQDPIVGYTNYPFFE 180
           PD   R E    LNKYDR+TFYT     GYT+YP +E
Sbjct: 354 PDLVYRLEEGLPLNKYDRSTFYTMS-AEGYTDYPTYE 389



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>OYE2_YEAST (Q03558) NADPH dehydrogenase 2 (EC 1.6.99.1) (Old yellow enzyme 2)|
          Length = 399

 Score = 54.3 bits (129), Expect = 2e-07
 Identities = 31/75 (41%), Positives = 41/75 (54%), Gaps = 3/75 (4%)
 Frame = -1

Query: 395 GTFIAAGGYDREEGNKVVADDYAD---LVAYGRIFLANPDFPKRFELNSALNKYDRNTFY 225
           G  I AG +      +VV ++  D   L+ YGR F++NPD   R E    LNKYDR+TFY
Sbjct: 318 GPIIRAGNFALHP--EVVREEVKDPRTLIGYGRFFISNPDLVDRLEKGLPLNKYDRDTFY 375

Query: 224 TQDPIVGYTNYPFFE 180
                 GY +YP +E
Sbjct: 376 KMS-AEGYIDYPTYE 389



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>OYE1_SACPS (Q02899) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yellow enzyme 1)|
          Length = 399

 Score = 54.3 bits (129), Expect = 2e-07
 Identities = 32/75 (42%), Positives = 42/75 (56%), Gaps = 3/75 (4%)
 Frame = -1

Query: 395 GTFIAAGGYDREEGNKVVADDYAD---LVAYGRIFLANPDFPKRFELNSALNKYDRNTFY 225
           G  I AG +      +VV ++  D   L+ YGR F++NPD   R E    LNKYDR+TFY
Sbjct: 318 GPVIRAGNFALHP--EVVREEVKDKRTLIGYGRFFISNPDLVDRLEKGLPLNKYDRDTFY 375

Query: 224 TQDPIVGYTNYPFFE 180
            Q    GY +YP +E
Sbjct: 376 -QMSAHGYIDYPTYE 389



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>KYE1_KLULA (P40952) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yellow enzyme 1)|
          Length = 398

 Score = 52.4 bits (124), Expect = 6e-07
 Identities = 40/119 (33%), Positives = 56/119 (47%), Gaps = 19/119 (15%)
 Frame = -1

Query: 464 RMAIVDGRTQIPHGLLPFRKAFDGTFIAAGG-----YDREEGNKVVADDYA--------- 327
           R+A VD     P    PF+  F+G +   GG     Y   +GN +   +YA         
Sbjct: 281 RLAYVD--LVEPRVTSPFQPEFEGWY--KGGTNEFVYSVWKGNVLRVGNYALDPDAAITD 336

Query: 326 -----DLVAYGRIFLANPDFPKRFELNSALNKYDRNTFYTQDPIVGYTNYPFFEGSNAE 165
                 L+ YGR F+ANPD  +R E    LN+YDR +FY      GY +YP +E + A+
Sbjct: 337 SKNPNTLIGYGRAFIANPDLVERLEKGLPLNQYDRPSFYKMS-AEGYIDYPTYEEAVAK 394



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>NEMA_ECOLI (P77258) N-ethylmaleimide reductase (EC 1.-.-.-) (N-ethylmaleimide|
           reducing enzyme)
          Length = 365

 Score = 50.4 bits (119), Expect = 2e-06
 Identities = 27/74 (36%), Positives = 37/74 (50%)
 Frame = -1

Query: 410 RKAFDGTFIAAGGYDREEGNKVVADDYADLVAYGRIFLANPDFPKRFELNSALNKYDRNT 231
           R  F G  I AG Y  E+   ++     D VA+GR ++ANPD   R +  + LN     +
Sbjct: 291 RARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAES 350

Query: 230 FYTQDPIVGYTNYP 189
           FY      GYT+YP
Sbjct: 351 FY-GGGAEGYTDYP 363



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>EBP1_CANAL (P43084) Probable NADPH dehydrogenase (EC 1.6.99.1)|
           (Estrogen-binding protein) (EBP)
          Length = 406

 Score = 42.4 bits (98), Expect = 6e-04
 Identities = 26/76 (34%), Positives = 38/76 (50%), Gaps = 4/76 (5%)
 Frame = -1

Query: 407 KAFDGTFIAAGGY--DREEGNKVVADDYAD--LVAYGRIFLANPDFPKRFELNSALNKYD 240
           K + G FI AG Y  D  E   ++ D   D  ++ + R F +NPD  ++ +L   LN Y+
Sbjct: 319 KIWKGNFIRAGNYTYDAPEFKTLINDLKNDRSIIGFSRFFTSNPDLVEKLKLGKPLNYYN 378

Query: 239 RNTFYTQDPIVGYTNY 192
           R  FY      GY +Y
Sbjct: 379 REEFYKYYN-YGYNSY 393



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>NADO_THEBR (P32382) NADH oxidase (EC 1.-.-.-)|
          Length = 651

 Score = 32.0 bits (71), Expect = 0.80
 Identities = 13/30 (43%), Positives = 21/30 (70%)
 Frame = -1

Query: 362 EEGNKVVADDYADLVAYGRIFLANPDFPKR 273
           E   K++A+  AD VA GR  +A+P++PK+
Sbjct: 303 EFAEKIIAEGRADFVAVGRGLIADPEWPKK 332



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>Y31K_SSV1 (P20203) Hypothetical 31.5 kDa protein (ORF B-277)|
          Length = 277

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 12/39 (30%), Positives = 21/39 (53%)
 Frame = +3

Query: 261 IELESLRKIWISQKDPSISNEISIVVGNHFVAFLSVIPS 377
           +E++ LRK W+   +P +   +    G  FVA   V+P+
Sbjct: 129 LEVKKLRKAWVLTAEPILLERLKFPFGKKFVAVHFVLPN 167



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>ALX_ECOLI (P42601) Protein alx|
          Length = 321

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 10/94 (10%)
 Frame = -2

Query: 400 LTALSLLLEG------MTERKATKWLPTTMLISLLMEGSFW--LIQIFLRDSSSIQP*TS 245
           + A+ LLL+G      MT ++A  W    + +SLL   +FW  L+Q   R  +  Q    
Sbjct: 20  MLAIDLLLQGRRGAHAMTMKQAAAWSLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAF 79

Query: 244 TTATLFIRKIL--SLATQIILFLKAQTPSSLSTR 149
            T  L  + +   ++   ++LF     P++L  R
Sbjct: 80  LTGYLIEKSLAVDNVFVWLMLFSYFSVPAALQRR 113



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>ALX_ECOL6 (Q8FDE1) Protein alx|
          Length = 321

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 10/94 (10%)
 Frame = -2

Query: 400 LTALSLLLEG------MTERKATKWLPTTMLISLLMEGSFW--LIQIFLRDSSSIQP*TS 245
           + A+ LLL+G      MT ++A  W    + +SLL   +FW  L+Q   R  +  Q    
Sbjct: 20  MLAIDLLLQGRRGAHAMTMKQAAAWSLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAF 79

Query: 244 TTATLFIRKIL--SLATQIILFLKAQTPSSLSTR 149
            T  L  + +   ++   ++LF     P++L  R
Sbjct: 80  LTGYLIEKSLAVDNVFVWLMLFSYFSVPAALQRR 113



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>ALX_ECO57 (Q8XAJ0) Protein alx|
          Length = 321

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 10/94 (10%)
 Frame = -2

Query: 400 LTALSLLLEG------MTERKATKWLPTTMLISLLMEGSFW--LIQIFLRDSSSIQP*TS 245
           + A+ LLL+G      MT ++A  W    + +SLL   +FW  L+Q   R  +  Q    
Sbjct: 20  MLAIDLLLQGRRGAHAMTMKQAAAWSLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAF 79

Query: 244 TTATLFIRKIL--SLATQIILFLKAQTPSSLSTR 149
            T  L  + +   ++   ++LF     P++L  R
Sbjct: 80  LTGYLIEKSLAVDNVFVWLMLFSYFSVPAALQRR 113



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>ALX_SHIFL (Q83Q35) Protein alx|
          Length = 320

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 10/94 (10%)
 Frame = -2

Query: 400 LTALSLLLEG------MTERKATKWLPTTMLISLLMEGSFW--LIQIFLRDSSSIQP*TS 245
           + A+ LLL+G      MT ++A  W    + +SLL   +FW  L+Q   R  +  Q    
Sbjct: 20  MLAIDLLLQGRRGAHAMTMKQAAAWSLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAF 79

Query: 244 TTATLFIRKIL--SLATQIILFLKAQTPSSLSTR 149
            T  L  + +   ++   ++LF     P++L  R
Sbjct: 80  LTGYLIEKSLAVDNVFVWLMLFSYFSVPAALQRR 113



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>GLYA_PSYAR (Q4FUZ8) Serine hydroxymethyltransferase (EC 2.1.2.1) (Serine|
           methylase) (SHMT)
          Length = 418

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 14/33 (42%), Positives = 20/33 (60%)
 Frame = -2

Query: 439 RKYPTGSCPSEKHLTALSLLLEGMTERKATKWL 341
           R Y   S  +E HL  +SL+ + MT ++A KWL
Sbjct: 303 RGYEIISGGTENHLMLISLVKQEMTGKEADKWL 335


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,916,652
Number of Sequences: 219361
Number of extensions: 1432795
Number of successful extensions: 3760
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 3700
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3759
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3014947676
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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